The Fibre Dimensions Of Uterine Smooth Muscle Of The Rabbit Following Treatment By Female Sex Steroids
Anatomy & Morphology; Cell Biology; estradiol; estrogen; gap-junctions; human myometrium; medroxyprogesterone; myometrium; parturition; Pregnancy; progesterone; rabbit; rat myometrium; receptors; smooth-muscle; uterus
Gilloteaux J; Szczepanski M
Tissue & Cell
2000
2000-06
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1054/tice.2000.0112" target="_blank" rel="noreferrer noopener">10.1054/tice.2000.0112</a>
Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis
cells; Endocrinology & Metabolism; endothelial growth-factor; estrogen; factor expression; nitric-oxide; parturition; rat; Reproductive Biology; tissue; tumor angiogenesis; vascular-permeability
Background: VEGF-regulated genes in the cervices of pregnant and non-pregnant rodents (rats and mice) were delineated by DNA microarray and Real Time PCR, after locally altering levels of or action of VEGF using VEGF agents, namely siRNA, VEGF receptor antagonist and mouse VEGF recombinant protein. Methods: Tissues were analyzed by genome-wide DNA microarray analysis, Real-time and gel-based PCR, and SEM, to decipher VEGF function during cervical remodeling. Data were analyzed by EASE score (microarray) and ANOVA (Real Time PCR) followed by Scheffe's F-test for multiple comparisons. Results: Of the 30,000 genes analyzed, about 4,200 genes were altered in expression by VEGF, i.e., expression of about 2,400 and 1,700 genes were down- and up-regulated, respectively. Based on EASE score, i.e., grouping of genes according to their biological process, cell component and molecular functions, a number of vascular- and non-vascular-related processes were found to be regulated by VEGF in the cervix, including immune response (including inflammatory), cell proliferation, protein kinase activity, and cell adhesion molecule activity. Of interest, mRNA levels of a select group of genes, known to or with potential to influence cervical remodeling were altered. For example, real time PCR analysis showed that levels of VCAM-1, a key molecule in leukocyte recruitment, endothelial adhesion, and subsequent trans-endothelial migration, were elevated about 10 folds by VEGF. Further, VEGF agents also altered mRNA levels of decorin, which is involved in cervical collagen fibrillogenesis, and expression of eNO, PLC and PKC mRNA, critical downstream mediators of VEGF. Of note, we show that VEGF may regulate cervical epithelial proliferation, as revealed by SEM. Conclusion: These data are important in that they shed new insights in VEGF's possible roles and mechanisms in cervical events near-term, including cervical remodeling.
Mowa C N; Li T B; Jesmin S; Folkesson H G; Usip S E; Papka R E; Hou G C
Reproductive Biology and Endocrinology
2008
2008-12
Journal Article
<a href="http://doi.org/10.1186/1477-7827-6-64" target="_blank" rel="noreferrer noopener">10.1186/1477-7827-6-64</a>
Mesenchymal stem cells and their secretome partially restore nerve and urethral function in a dual muscle and nerve injury stress urinary incontinence model.
*Mesenchymal Stem Cell Transplantation; Animals; Conditioned; Culture Media; elastin; external urethral sphincter; Female; Injections; Intraperitoneal; Intravenous; Mesenchymal Stem Cells/metabolism; paracrine action; Parturition; pudendal nerve; Pudendal Nerve/injuries/*physiology; Rats; Sprague-Dawley; Stress/etiology/*prevention & control; Urethra/injuries/*physiology; urinary incontinence; Urinary Incontinence
Childbirth injures muscles and nerves responsible for urinary continence. Mesenchymal stem cells (MSCs) or their secretome given systemically could provide therapeutic benefit for this complex multisite injury. We investigated whether MSCs or their secretome, as collected from cell culture, facilitate recovery from simulated childbirth injury. Age-matched female Sprague-Dawley rats received pudendal nerve crush and vaginal distension (PNC+VD) and a single intravenous (iv) injection of 2 million MSCs or saline. Controls received sham injury and iv saline. Additional rats received PNC+VD and a single intraperitoneal (ip) injection of concentrated media conditioned by MSCs (CCM) or concentrated control media (CM). Controls received a sham injury and ip CM. Urethral and nerve function were assessed with leak point pressure (LPP) and pudendal nerve sensory branch potential (PNSBP) recordings 3 wk after injury. Urethral and pudendal nerve anatomy were assessed qualitatively by blinded investigators. Quantitative data were analyzed using one-way ANOVA and Holm-Sidak post hoc tests with P \textless 0.05 indicating significant differences. Both LPP and PNSBP were significantly decreased 3 wk after PNC+VD with saline or CM compared with sham-injured rats, but not with MSC or CCM. Elastic fiber density in the urethra increased and changed in orientation after PNC+VD, with a greater increase in elastic fibers with MSC or CCM. Pudendal nerve fascicles were less dense and irregularly shaped after PNC+VD and had reduced pathology with MSC or CCM. MSC and CCM provide similar protective effects after PNC+VD, suggesting that MSCs act via their secretions in this dual muscle and nerve injury.
Deng Kangli; Lin Dan Li; Hanzlicek Brett; Balog Brian; Penn Marc S; Kiedrowski Matthew J; Hu Zhiquan; Ye Zhangqun; Zhu Hui; Damaser Margot S
American journal of physiology. Renal physiology
2015
2015-01
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1152/ajprenal.00510.2014" target="_blank" rel="noreferrer noopener">10.1152/ajprenal.00510.2014</a>