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Text
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<a href="http://doi.org/10.3390/ijms21165675" target="_blank" rel="noreferrer noopener">http://doi.org/10.3390/ijms21165675</a>
Issue
16
Volume
21
ISSN
1422-0067 1422-0067
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Update Year & Number
August 2020 List
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
NEOMED Postdoc Publications
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Title
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Small non-coding RNAome of ageing chondrocytes.
Publisher
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International Journal of Molecular Sciences
Date
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2020
2020-08-07
Subject
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chondrocyte; ageing; equine; small non-coding RNA
Creator
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Balaskas P; Green JA; Haqqi TM; Dyer P; Kharaz YA; Fang Y; Liu X; Welting TJM; Peffers MJ
Description
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Ageing is a leading risk factor predisposing cartilage to osteoarthritis. However, little research has been conducted on the effect of ageing on the expression of small non-coding RNAs (sncRNAs). RNA from young and old chondrocytes from macroscopically normal equine metacarpophalangeal joints was extracted and subjected to small RNA sequencing (RNA-seq). Differential expression analysis was performed in R using package DESeq2. For transfer RNA (tRNA) fragment analysis, tRNA reads were aligned to horse tRNA sequences using Bowtie2 version 2.2.5. Selected microRNA (miRNAs or miRs) and small nucleolar RNA (snoRNA) findings were validated using real-time quantitative Polymerase Chain Reaction (qRT-PCR) in an extended cohort of equine chondrocytes. tRNA fragments were further investigated in low- and high-grade OA human cartilage tissue. In total, 83 sncRNAs were differentially expressed between young and old equine chondrocytes, including miRNAs, snoRNAs, small nuclear RNAs (snRNAs), and tRNAs. qRT-PCR analysis confirmed findings. tRNA fragment analysis revealed that tRNA halves (tiRNAs), tiRNA-5035-GluCTC and tiRNA-5031-GluCTC-1 were reduced in both high grade OA human cartilage and old equine chondrocytes. For the first time, we have measured the effect of ageing on the expression of sncRNAs in equine chondrocytes. Changes were detected in a number of different sncRNA species. This study supports a role for sncRNAs in ageing cartilage and their potential involvement in age-related cartilage diseases.
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<a href="http://doi.org/10.3390/ijms21165675" target="_blank" rel="noreferrer noopener">10.3390/ijms21165675</a>
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journalArticle
2020
ageing
August 2020 List
Balaskas P
chondrocyte
Department of Anatomy & Neurobiology
Dyer P
equine
Fang Y
Green JA
Haqqi TM
International journal of molecular sciences
journalArticle
Kharaz YA
Liu X
NEOMED College of Medicine
NEOMED College of Medicine Postdoc
NEOMED Postdoc Publications
Peffers MJ
small non-coding RNA
Welting TJM