1
40
1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.3109/10837450.2015.1041043" target="_blank" rel="noreferrer noopener">http://doi.org/10.3109/10837450.2015.1041043</a>
Pages
647–654
Issue
6
Volume
21
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Gelucire-stabilized nanoparticles as a potential DNA delivery system.
Publisher
An entity responsible for making the resource available
Pharmaceutical development and technology
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-09
Subject
The topic of the resource
Humans; Animals; Mice; nanoparticles; Cell Line; Hep G2 Cells; gene therapy; *Gene Transfer Techniques; Cationic lipids; Cell Survival/drug effects/physiology; DNA/*administration & dosage/chemistry; macrophage activation; Macrophages/drug effects/physiology; Nanoparticles/*administration & dosage/chemistry; Polyethylene Glycols/*administration & dosage/chemistry; transfection
Creator
An entity primarily responsible for making the resource
Oyewumi Moses O; Wehrung Daniel; Sadana Prabodh
Description
An account of the resource
Clinical viability of gene delivery systems has been greatly impacted by potential toxicity of the delivery systems. Recently, we reported the nanoparticle (NP) preparation process that employs biocompatible materials such as Gelucire(R) 44/14 and cetyl alcohol as matrix materials. In the current study, the NP preparation was modified for pDNA loading through: (i) inclusion of cationic lipids (DOTAP or DDAB) with NP matrix materials; or (ii) application of cationic surfactants (CTAB) to generate NPs with desired surface charges for pDNA complexation. Colloidal stability and efficiency of loading pGL3-DR4X2-luciferase plasmid DNA in NPs were verified by gel permeation chromatography. Compared to pDNA alone, all the NPs were effective in preserving pDNA from digestion by DNase. While pDNA loading using CTAB-NPs involved fewer steps compared to
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.3109/10837450.2015.1041043" target="_blank" rel="noreferrer noopener">10.3109/10837450.2015.1041043</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Transfer Techniques
2016
Animals
Cationic lipids
Cell Line
Cell Survival/drug effects/physiology
Department of Pharmaceutical Sciences
Department of Pharmacy Practice
DNA/*administration & dosage/chemistry
gene therapy
Hep G2 Cells
Humans
macrophage activation
Macrophages/drug effects/physiology
Mice
Nanoparticles
Nanoparticles/*administration & dosage/chemistry
NEOMED College of Graduate Studies
NEOMED College of Pharmacy
Oyewumi Moses O
Pharmaceutical development and technology
Polyethylene Glycols/*administration & dosage/chemistry
Sadana Prabodh
Transfection
Wehrung Daniel