1
40
2
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
3889–3897
Issue
7
Volume
265
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of cholesterol 7 alpha-hydroxylase in the liver. Purification of cholesterol 7 alpha-hydroxylase and the immunochemical evidence for the induction of cholesterol 7 alpha-hydroxylase by cholestyramine and circadian rhythm.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1990
1990-03
Subject
The topic of the resource
Animals; Rats; Organ Specificity; Kinetics; *Circadian Rhythm; Enzyme Induction; Liver/drug effects/*enzymology; Cholesterol 7-alpha-Hydroxylase/*biosynthesis/isolation & purification/metabolism; Cholestyramine Resin/*pharmacology; Chromatography; Durapatite; Hydroxyapatites; Isoenzymes/*biosynthesis/isolation & purification; Obesity/enzymology; Polyethylene Glycols; Steroid Hydroxylases/*biosynthesis; Inbred Strains; Zucker; Microsomes; Ion Exchange
Creator
An entity primarily responsible for making the resource
Chiang J Y; Miller W F; Lin G M
Description
An account of the resource
Two cholesterol 7 alpha-hydroxylase isozymes were purified from liver microsomes of cholestyramine-treated female rats by using anion exchange high performance liquid chromatography. These two cytochrome P-450 isozymes were similar in electrophoretic mobility, immunocross-reactivity, and Vmax but differed in Km for cholesterol, turnover number, and charges. Antibody against the major isozyme was raised in rabbit. This antibody specifically inhibited microsomal cholesterol 7 alpha-hydroxylase activity. Immunoblot of microsomal polypeptides indicated that microsomal cholesterol 7 alpha-hydroxylase enzyme levels were increased in parallel with cholesterol 7 alpha-hydroxylase activity upon the treatment of rats with diet supplemented with cholestyramine. Both cholesterol 7 alpha-hydroxylase activity and enzyme levels were drastically reduced immediately after the removal of cholestyramine from the diet. Cholesterol 7 alpha-hydroxylase activity was also detected in the microsomes of kidney, heart, and lung in about 7-27% of the level found in the liver. 3-Methylcholanthrene treatment induced cholesterol 7 alpha-hydroxylase activity and enzyme level. In contrast, pregnenolone-16 alpha-carbonitrile or dexamethasone treatment greatly depressed enzyme and activity in rats. Cholesterol 7 alpha-hydroxylase enzyme level was 2-3-fold higher in liver microsomes of rats maintained under the reversed light cycle than under the normal light cycle. In genetically obese Zucker rats, cholesterol 7 alpha-hydroxylase activity and enzyme level did not respond to the change in the light cycle, however, were induced to the same levels as in the lean rats by cholestyramine treatment. This study provided the first direct evidence that the bile acid feedback regulation and circadian rhythm of microsomal cholesterol 7 alpha-hydroxylase activity involved the induction of cholesterol 7 alpha-hydroxylase enzyme level.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Circadian Rhythm
1990
Animals
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*biosynthesis/isolation & purification/metabolism
Cholestyramine Resin/*pharmacology
Chromatography
Department of Integrative Medical Sciences
Durapatite
Enzyme Induction
Hydroxyapatites
Inbred Strains
Ion Exchange
Isoenzymes/*biosynthesis/isolation & purification
Kinetics
Lin G M
Liver/drug effects/*enzymology
Microsomes
Miller W F
NEOMED College of Medicine
Obesity/enzymology
Organ Specificity
Polyethylene Glycols
Rats
Steroid Hydroxylases/*biosynthesis
The Journal of biological chemistry
Zucker
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.3109/10611861003639796" target="_blank" rel="noreferrer noopener">http://doi.org/10.3109/10611861003639796</a>
Pages
665–674
Issue
9
Volume
18
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Brain-targeted delivery of Tempol-loaded nanoparticles for neurological disorders.
Publisher
An entity responsible for making the resource available
Journal of drug targeting
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
2010-11
Subject
The topic of the resource
Animals; Rats; Cell Line; Nanoparticles; Polylactic Acid-Polyglycolic Acid Copolymer; Particle Size; Delayed-Action Preparations; Antibodies; Polyethylene Glycols; Blood-Brain Barrier/metabolism; *Lactic Acid; *Polyglycolic Acid; Antioxidants/chemistry/*metabolism; Cross-Linking Reagents/chemistry; Cyclic N-Oxides/chemistry/*metabolism; Free Radical Scavengers/chemistry/*metabolism; Maleimides/chemistry; Spin Labels; Transferrin/*immunology; Tumor; Monoclonal/chemistry/*metabolism
Creator
An entity primarily responsible for making the resource
Carroll Richard T; Bhatia Deepak; Geldenhuys Werner; Bhatia Ruchi; Miladore Nicholas; Bishayee Anupam; Sutariya Vijaykumar
Description
An account of the resource
Brain-targeted Tempol-loaded poly-(lactide-co-glycolide) (PLGA) nanoparticles (NPs) conjugated with a transferrin antibody (OX 26) were developed using the nanoprecipitation method. These NPs may have utility in treating neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Central to these diseases is an increased production of reactive oxygen and nitrogen species which may take part in the development of these conditions. As proof of principle, the NPs were loaded with Tempol, a free radical scavenger that has been shown to be protective against oxidative insults. To enhance the delivery of NPs to the central nervous system (CNS), we conjugated the transferrin receptor antibody covalently to PLGA NPs using the
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.3109/10611861003639796" target="_blank" rel="noreferrer noopener">10.3109/10611861003639796</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Lactic Acid
*Polyglycolic Acid
2010
Animals
Antibodies
Antioxidants/chemistry/*metabolism
Bhatia Deepak
Bhatia Ruchi
Bishayee Anupam
Blood-Brain Barrier/metabolism
Carroll Richard T
Cell Line
Cross-Linking Reagents/chemistry
Cyclic N-Oxides/chemistry/*metabolism
Delayed-Action Preparations
Free Radical Scavengers/chemistry/*metabolism
Geldenhuys Werner
Journal of drug targeting
Maleimides/chemistry
Miladore Nicholas
Monoclonal/chemistry/*metabolism
Nanoparticles
Particle Size
Polyethylene Glycols
Polylactic Acid-Polyglycolic Acid Copolymer
Rats
Spin Labels
Sutariya Vijaykumar
Transferrin/*immunology
Tumor