Binding of sequence-specific proteins to the 3'-untranslated region of vasoactive intestinal peptide mRNA.
Animals; Anterior/*metabolism; Base Sequence; Binding; Competitive; Cytoplasm/chemistry; Gene Expression Regulation; Genomic Library; Male; Messenger/*metabolism; Molecular Sequence Data; Pituitary Gland; Post-Transcriptional; Protein Binding; Proto-Oncogene Proteins c-fos/genetics; Rats; RNA; RNA Processing; RNA-Binding Proteins/*metabolism; RNA/metabolism; Sprague-Dawley; Subcellular Fractions; Tissue Distribution; Vasoactive Intestinal Peptide/*genetics
Multimeric AUUUA elements in an AU-rich 3'-untranslated region (3'-UTR) have been shown to confer message instability to numerous ephemeral transcripts through the formation of RNA-protein complexes. We show here that the 3'-UTR of VIP mRNA, which contains 3 AUUUA motifs in an AU-rich context, forms specific complexes with cytoplasmic proteins in a concentration-dependent, tissue-specific manner. We also demonstrate that an AU-rich segment of c-fos mRNA can successfully compete with VIP mRNA for binding with cytoplasmic proteins. These studies provide the first evidence for a mechanism by which VIP is post-transcriptionally regulated through specific sequences in its 3'-UTR.
Wolford J K; Signs S A
Biochemical and biophysical research communications
1995
1995-06
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1006/bbrc.1995.1885" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1995.1885</a>
The characterization of three types of partially processed mRNA and two pseudogenes for human liver cytochrome b5.
Humans; Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Liver/*physiology; DNA/genetics; Cytochrome b Group/*genetics; Cytochromes b5; Pseudogenes; Cloning; Molecular; Post-Transcriptional; RNA Processing
We have isolated cDNA clones corresponding to partially processed human liver cytochrome b5 mRNAs. All the clones contained poly(A) sequences, and one clone had a shorter 3' non-translated sequence, indicating the use of an alternative poly(A) addition signal. In addition, all the clones contained the coding information for amino acids 87-134; however, there were two types of intron junction adjacent to the coding sequence. Detailed analysis of the Type I clones showed that the Type II intron sequence was contained within the Type I sequence, but approximately 1000 bp 5' of the Type I intron-exon junction showed alternative splicing within this intron. In addition, we have isolated two pseudogenes which lack introns, suggesting the retroviral insertion of human liver cytochrome b5 mRNA sequences into the human genome.
Yoo M; Steggles A W
Biochemical and biophysical research communications
1989
1989-08
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0006-291x(89)92092-5" target="_blank" rel="noreferrer noopener">10.1016/0006-291x(89)92092-5</a>