Norepinephrine is lateralized within the olfactory bulbs of male mice.
*Functional Laterality; *Sex Characteristics; Animals; Dopamine/metabolism; Inbred Strains; Male; Mice; Norepinephrine/*metabolism; Olfactory Bulb/*metabolism; Osmolar Concentration; Potassium/pharmacology; Tissue Distribution
Concentrations and in vitro release of norepinephrine were determined from left and right olfactory bulbs of adult male CD-1 mice. Norepinephrine concentrations from the left olfactory bulbs were significantly greater than those of the right (p \textless 0.01). No statistically significant differences were obtained for dopamine, nor were there differences in tissue weights between the left and right olfactory bulb tissue samples. To verify further this asymmetry, release rates of norepinephrine were measured from superfused left and right olfactory bulbs. Norepinephrine output from the left olfactory bulb was significantly greater than that from the right (p \textless 0.05). These results show that norepinephrine is lateralized within the olfactory bulbs of male mice. This asymmetry shows a clear catecholamine specificity with regard to concentrations and may be related to the lateralized olfactory processing that has been reported to occur in humans.
Dluzen D E; Kreutzberg J D
Journal of neurochemistry
1996
1996-03
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1046/j.1471-4159.1996.66031222.x" target="_blank" rel="noreferrer noopener">10.1046/j.1471-4159.1996.66031222.x</a>
The effect of testosterone upon methamphetamine neurotoxicity of the nigrostriatal dopaminergic system.
Animals; Corpus Striatum/drug effects/*metabolism; Dopamine/*metabolism; Drug Implants; Female; Male; Methamphetamine/*toxicity; Mice; Neuroprotective Agents/pharmacology; Neurotoxins/*toxicity; Orchiectomy; Ovariectomy; Potassium/pharmacology; Substantia Nigra/drug effects/*metabolism; Testosterone/administration & dosage/*pharmacology
The gonadal steroid hormone estrogen (E) can function as a neuroprotectant of nigrostriatal dopaminergic (NSDA) neurotoxicity, however, there exists very limited information on the role of testosterone (T) in this capacity. In the present report, the effects of T on methamphetamine (MA) induced neurotoxicity of the NSDA system were examined in gonadectomized female and male CD-1 mice. In Experiment 1, striatal dopamine (DA) concentrations and output from T-treated ovariectomized mice were not significantly different from that of non-T-treated mice following MA. These results suggest that T is not functioning as a modulator of MA-induced NSDA neurotoxicity in ovariectomized CD-1 mice. In Experiment 2, there were no significant differences in DA concentrations or output among
Gao X; Dluzen D E
Brain research
2001
2001-02
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/s0006-8993(00)03221-2" target="_blank" rel="noreferrer noopener">10.1016/s0006-8993(00)03221-2</a>
Sex differences in methamphetamine-evoked striatal dopamine of mice are reversed by nomifensine.
*Sex Characteristics; Animals; Corpus Striatum/*drug effects; Dopamine Agents/pharmacology; Dopamine Uptake Inhibitors/*pharmacology; Dopamine/*metabolism; Drug Interactions; Female; Male; Methamphetamine/pharmacology; Mice; Nomifensine/*pharmacology; Potassium/pharmacology
Male mice show more severe striatal dopamine depletions to the psychostimulant, methamphetamine (MA). To gain some understanding for this sex difference, we examined MA-evoked dopamine (DA) responses from superfused striatal tissue fragments of male and female mice under conditions of a dopamine transporter which was either unaltered (Experiment 1) or inhibited, with use of the drug, nomifensine (Experiment 2). In Experiment 1, MA-evoked DA was significantly greater in male versus female mice. In Experiment 2, diminished, albeit statistically significant, DA responses to MA infusion in the presence of nomifensine were obtained from striatal tissue of female, but not male, mice. In Experiment 3, potassium-evoked DA responses and sex differences were abolished in the presence of nomifensine. These data demonstrate a clear sex difference in DA responses to MA. Interestingly, under conditions where dopamine transporter function is inhibited, MA retains its ability to evoke DA. However, this capacity was only observed within striatal tissue fragments of female mice and not under conditions of potassium-evoked DA. These results indicate an additional component for the bases of sex differences in nigrostriatal dopaminergic function in health and in disease. In particular, the present findings have important implications in suggesting an alternative, non-traditional, mechanism for MA effects and indicate that such a function is limited to females.
Kunnathur Vidhya; Shemisa Kamal; Liu Bin; Salvaterra Ty J; Dluzen Dean E
Neurotoxicology and teratology
2006
2006-10
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/j.ntt.2006.07.001" target="_blank" rel="noreferrer noopener">10.1016/j.ntt.2006.07.001</a>
Aging and sex differences in striatal dopaminergic function.
3; 4-Dihydroxyphenylacetic Acid/metabolism; Aging/*physiology; Amphetamine/pharmacology; Animals; Chemical; Dopamine Uptake Inhibitors/pharmacology; Dopamine/metabolism/*physiology; Female; In Vitro Techniques; Male; Mice; Neostriatum/metabolism/*physiology; Organ Size/physiology; Potassium/pharmacology; Sex Characteristics; Stimulation; Uterus/physiology
In this report the potassium- (30 mM) and amphetamine- (10 microM) stimulated responses of dopamine (DA) and 3,4-dihydroxy phenylacetic acid (DOPAC) from superfused striatal tissue of female and male mice as sampled at 2, 6, 18 and 24 months of age were compared. When assessed relative to responses obtained from
McDermott J L; Dluzen D E
Neuroscience
2007
2007-10
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/j.neuroscience.2007.06.058" target="_blank" rel="noreferrer noopener">10.1016/j.neuroscience.2007.06.058</a>
The effect of estrogen administration in vivo upon catecholamine release in vitro from superfused hypothalamic tissue of ovariectomized pre-pubertal and adult mice.
*Ovariectomy; Aging/*metabolism; Animals; Catecholamines/*metabolism; Dopamine/metabolism; Estradiol/*pharmacology; Female; Hypothalamus/*drug effects/metabolism; Mice; Norepinephrine/metabolism; Organ Size/drug effects; Potassium/pharmacology; Uterus/drug effects/growth & development
In the present report we examined the effects of estrogen upon catecholamine release from superfused medial basal hypothalamic tissue fragments of pre-pubertal ovariectomized CD-1 mice. Prepubertal mice treated with estradiol benzoate (EB–5 micrograms x 2 days, sc), showed significantly reduced amounts of dopamine but no changes in norepinephrine release in response to a depolarizing concentration of potassium (30 mmol/L) compared with their respective groups receiving the oil vehicle. Since EB treatment reduced potassium stimulated dopamine release in these pre-pubertal mice, in a second experiment we compared the effects of EB versus oil vehicle treatment upon potassium stimulated dopamine release from the hypothalamus of the ovariectomized adult female mouse. Similar to that observed in the pre-pubertal mouse, EB treatment significantly reduced the amount of potassium stimulated dopamine release. Interestingly, the absolute amounts of potassium stimulated dopamine release was substantially greater in adult compared with pre-pubertal mice. These results demonstrate that the hypothalamic dopaminergic system of both pre-pubertal and adult mice show relatively similar responses to estrogen treatment but differ in absolute amounts of dopamine released.
Dluzen D; Attaran M; Liu B
Journal of endocrinological investigation
1994
1994-12
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1007/BF03347791" target="_blank" rel="noreferrer noopener">10.1007/BF03347791</a>
L-dopa infusion mode differentially affects corpus striatal dopamine efflux in the presence of reserpine.
Amphetamine/pharmacology; Animals; Chromatography; Corpus Striatum/*drug effects/metabolism; Dopamine/*metabolism; High Pressure Liquid; Infusions; Intravenous; Levodopa/*pharmacology; Male; Potassium/pharmacology; Rats; Reserpine/*pharmacology; Sprague-Dawley
In the present experiment we tested the effects of L-DOPA upon dopamine (DA) efflux in vitro from superfused corpus striatal tissue fragments in medium containing reserpine. The purposes of this experiment were first, to evaluate the effects of differing infusion modes of L-DOPA upon DA efflux under conditions in which DA storage capacity has been diminished, and second, to compare this L-DOPA stimulated DA efflux with that of other putative DA secretagogues such as amphetamine and potassium. No differences were obtained in stimulated DA efflux between superfusions performed in the presence or absence of reserpine (10 microM) in the medium when L-DOPA (5 microM) was infused in a continuous (70 minute) mode during the superfusion. In contrast, a continuous infusion of either amphetamine (10 microM) or high potassium (30 mM) resulted in significantly greater stimulated DA efflux in superfusions performed with reserpine in the medium. In addition, when L-DOPA (5 microM) was administered for a brief
Dluzen D E; Kratko F T Jr
Journal of neural transmission. General section
1992
1905-6
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1007/bf01250672" target="_blank" rel="noreferrer noopener">10.1007/bf01250672</a>