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40
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jnr.21225" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jnr.21225</a>
Pages
1318–1335
Issue
6
Volume
85
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Glutamate and metabotropic glutamate receptors associated with innervation of the uterine cervix during pregnancy: receptor antagonism inhibits c-Fos expression in rat lumbosacral spinal cord at parturition.
Publisher
An entity responsible for making the resource available
Journal of neuroscience research
Date
A point or period of time associated with an event in the lifecycle of the resource
2007
2007-05
Subject
The topic of the resource
Animals; Cervix Uteri/*innervation; Excitatory Amino Acid Antagonists/pharmacology; Female; Ganglia; Gene Expression Regulation/drug effects/*physiology; Glutamates/*metabolism; Lumbosacral Region; Messenger/biosynthesis; Metabotropic Glutamate 5; Metabotropic Glutamate/*metabolism; Neurons/drug effects; Oncogene Proteins v-fos/metabolism; Parturition/*metabolism; Pregnancy/*metabolism; Pyridines/pharmacology; Rats; Receptor; Receptors; Reverse Transcriptase Polymerase Chain Reaction/methods; RNA; Spinal Cord/drug effects/*physiology; Spinal/cytology; Sprague-Dawley; Vesicular Glutamate Transport Protein 1/metabolism
Creator
An entity primarily responsible for making the resource
Ghosh Chaitali; Storey-Workley Megan; Usip Sharon; Hafemeister Jen; Miller Kenneth E; Papka Raymond E
Description
An account of the resource
Dorsal root ganglia (DRG) neurons connect the spinal cord and uterine cervix, and are activated at parturition with subsequent stimulation of secondary neurons in the spinal dorsal horn and autonomic areas. Neuropeptide neurotransmitters and receptors have been studied in these areas, but amino acid transmitters, e.g., glutamate, an excitatory neurotransmitter involved in sensory and nociceptive processing, have not been characterized. To determine if glutamate is involved in innervation of the cervix, rats were examined for markers of glutamatergic neurons in the L6-S1 spinal cord, DRG and cervix. Metabotropic glutamate receptors mGluR5 in the spinal dorsal horn and their expression over pregnancy were examined in pregnant rats and pregnant rats treated continuously with an antagonist of mGluR5, 2-methyl-6-(phenylethynyl) pyridine (MPEP). Rats were allowed to deliver pups to determine if the antagonist altered the expression of an early response gene protein, Fos, in the L6-S1 cord. Immunohistochemistry showed glutamate- and vesicular glutamate transporter1 (VGluT1)-positive fibers in the cervix, glutamate- and VGluT1-expressing neurons in the DRG, some of which also exhibited retrograde tracer from cervical injections, and VGluT1 and mGluR5 immunoreactivities in the L6-S1 spinal dorsal horns. Expression of mGluR5 receptors increased over pregnancy. Fos-positive neurons were present among mGluR5-immunoreactivity in the spinal dorsal horn. Parturition-induced Fos-positive neurons in the spinal cords were abundant in control rats, but were reduced by 70% in MPEP-treated animals. These results suggest that glutamate is likely involved in the transmission of sensory signals, possibly pain, from the cervix to the spinal cord at parturition.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/jnr.21225" target="_blank" rel="noreferrer noopener">10.1002/jnr.21225</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2007
Animals
Cervix Uteri/*innervation
Department of Anatomy & Neurobiology
Excitatory Amino Acid Antagonists/pharmacology
Female
Ganglia
Gene Expression Regulation/drug effects/*physiology
Ghosh Chaitali
Glutamates/*metabolism
Hafemeister Jen
Journal of neuroscience research
Lumbosacral Region
Messenger/biosynthesis
Metabotropic Glutamate 5
Metabotropic Glutamate/*metabolism
Miller Kenneth E
NEOMED College of Medicine
Neurons/drug effects
Oncogene Proteins v-fos/metabolism
Papka Raymond E
Parturition/*metabolism
Pregnancy/*metabolism
Pyridines/pharmacology
Rats
Receptor
Receptors
Reverse Transcriptase Polymerase Chain Reaction/methods
RNA
Spinal Cord/drug effects/*physiology
Spinal/cytology
Sprague-Dawley
Storey-Workley Megan
Usip Sharon
Vesicular Glutamate Transport Protein 1/metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0196-9781(03)00120-7" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0196-9781(03)00120-7</a>
Pages
761–771
Issue
5
Volume
24
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Substance P in the uterine cervix, dorsal root ganglia and spinal cord during pregnancy and the effect of estrogen on SP synthesis.
Publisher
An entity responsible for making the resource available
Peptides
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-05
Subject
The topic of the resource
Afferent/metabolism; Animals; Cervix Uteri/cytology/*metabolism; Down-Regulation; Estradiol/*analogs & derivatives/pharmacology; Estrogen/antagonists & inhibitors; Estrogens/*pharmacology; Female; Fulvestrant; Ganglia; Immunohistochemistry; In Situ Hybridization; Messenger/genetics/metabolism; Neurons; Postpartum Period; Pregnancy/*metabolism; Radioimmunoassay; Rats; Receptors; Reverse Transcriptase Polymerase Chain Reaction; RNA; Spinal Cord/cytology/*metabolism; Spinal/cytology/*metabolism; Sprague-Dawley; Substance P/*biosynthesis/genetics/metabolism; Up-Regulation
Creator
An entity primarily responsible for making the resource
Mowa C N; Usip S; Storey-Workley M; Amann R; Papka R
Description
An account of the resource
Prior to parturition the non-pliable uterine cervix undergoes a ripening process ("softens" and dilates) to allow a timely passage of the fetus at term. The exact mechanism(s) triggering and involved in cervical ripening are unknown, though evidence for a role for sensory neurons and their contained neuropeptides is emerging. Moreover, an apparent increase in neuropeptide immunoreactive nerves occurs in the cervix during pregnancy, maternal serum estrogen levels rise at term and uterine cervix-related L6-S1 dorsal root ganglia (DRG) sensory neurons express estrogen receptor (ER) and neuropeptides. Thus, we sought to test the hypothesis that the neuropeptide substance P (SP) changes biosynthesis and release over pregnancy, that estrogen, acting via the ER pathway, increases synthesis of SP in DRG, and that SP is utilized in cervical ripening at late pregnancy. Using immunohistochemistry, in situ hybridization, reverse transcriptase-polymerase chain reaction (RT-PCR) and radioimmunoassay (RIA), we investigated coexpression of ER-alpha/beta and SP; differential expression of
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0196-9781(03)00120-7" target="_blank" rel="noreferrer noopener">10.1016/s0196-9781(03)00120-7</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2003
Afferent/metabolism
Amann R
Animals
Cervix Uteri/cytology/*metabolism
Department of Anatomy & Neurobiology
Down-Regulation
Estradiol/*analogs & derivatives/pharmacology
Estrogen/antagonists & inhibitors
Estrogens/*pharmacology
Female
Fulvestrant
Ganglia
Immunohistochemistry
In Situ Hybridization
Messenger/genetics/metabolism
Mowa C N
NEOMED College of Medicine
Neurons
Papka R
Peptides
Postpartum Period
Pregnancy/*metabolism
Radioimmunoassay
Rats
Receptors
Reverse Transcriptase Polymerase Chain Reaction
RNA
Spinal Cord/cytology/*metabolism
Spinal/cytology/*metabolism
Sprague-Dawley
Storey-Workley M
Substance P/*biosynthesis/genetics/metabolism
Up-Regulation
Usip S