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Text
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URL Address
<a href="http://doi.org/10.1074/jbc.M114.598698" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M114.598698</a>
Pages
1936–1951
Issue
4
Volume
290
Dublin Core
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Title
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Generation and characterization of ATP analog-specific protein kinase Cdelta.
Publisher
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The Journal of biological chemistry
Date
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2015
2015-01
Subject
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Adenosine Triphosphate/*analogs & derivatives/*chemistry; Amino Acid; Amino Acid Sequence; Animals; ATP; Catalysis; Cercopithecus aethiops; Chemical Biology; COS Cells; Glutamine/chemistry; Humans; Inbred C57BL; Leucine/chemistry; Lysine/chemistry; Mice; Molecular Sequence Data; Neutrophils/metabolism; Phenylalanine/chemistry; Phosphorylation; Protein Binding; Protein Kinase C (PKC); Protein Kinase C-delta/*metabolism; Protein Phosphorylation; Purines/chemistry; Sequence Homology; Signal Transduction; Stroke; Substrate Specificity; Superoxides/chemistry; Transgenic
Creator
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Kumar Varun; Weng Yi-Chinn; Geldenhuys Werner J; Wang Dan; Han Xiqian; Messing Robert O; Chou Wen-Hai
Description
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To better study the role of PKCdelta in normal function and disease, we developed an ATP analog-specific (AS) PKCdelta that is sensitive to specific kinase inhibitors and can be used to identify PKCdelta substrates. AS PKCdelta showed nearly 200 times higher affinity (Km) and 150 times higher efficiency (kcat/Km) than wild type (WT) PKCdelta toward N(6)-(benzyl)-ATP. AS PKCdelta was uniquely inhibited by 1-(tert-butyl)-3-(1-naphthyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1NA-PP1) and
Identifier
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<a href="http://doi.org/10.1074/jbc.M114.598698" target="_blank" rel="noreferrer noopener">10.1074/jbc.M114.598698</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2015
Adenosine Triphosphate/*analogs & derivatives/*chemistry
Amino Acid
Amino Acid Sequence
Animals
ATP
Catalysis
Cercopithecus aethiops
Chemical Biology
Chou Wen-Hai
COS Cells
Geldenhuys Werner J
Glutamine/chemistry
Han Xiqian
Humans
Inbred C57BL
Kumar Varun
Leucine/chemistry
Lysine/chemistry
Messing Robert O
Mice
Molecular Sequence Data
Neutrophils/metabolism
Phenylalanine/chemistry
Phosphorylation
Protein Binding
Protein Kinase C (PKC)
Protein Kinase C-delta/*metabolism
Protein Phosphorylation
Purines/chemistry
Sequence Homology
Signal Transduction
stroke
Substrate Specificity
Superoxides/chemistry
The Journal of biological chemistry
Transgenic
Wang Dan
Weng Yi-Chinn