1
40
5
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.taap.2018.09.011" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.taap.2018.09.011</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
102-107
Volume
359
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Quinone and nitrofurantoin redox cycling by recombinant cytochrome b5 reductase.
Publisher
An entity responsible for making the resource available
Toxicology and applied pharmacology
Date
A point or period of time associated with an event in the lifecycle of the resource
2018
2018-11
Subject
The topic of the resource
Humans; Reactive Oxygen Species/metabolism; Oxidation-Reduction; Liver; *Reactive oxygen species; Kinetics; Recombinant Proteins/metabolism; Oxygen Consumption; Free Radicals/metabolism; Microsomes; *Cytochrome b5; *Free radicals; *Redox cycling; Benzoquinones/*metabolism; Cytochrome-B(5) Reductase/*metabolism; NADP/metabolism; Nitrofurantoin/*metabolism
Creator
An entity primarily responsible for making the resource
Szilagyi John T; Fussell Karma C; Wang Yun; Jan Yi-Hua; Mishin Vladimir; Richardson Jason R; Heck Diane E; Yang Shaojun; Aleksunes Lauren M; Laskin Debra L; Laskin Jeffrey D
Description
An account of the resource
NADH cytochrome b5 reductase mediates electron transfer from NADH to cytochrome b5 utilizing flavin adenine dinucleotide as a redox cofactor. Reduced cytochrome b5 is an important cofactor in many metabolic reactions including cytochrome
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.taap.2018.09.011" target="_blank" rel="noreferrer noopener">10.1016/j.taap.2018.09.011</a>
*Cytochrome b5
*Free radicals
*Reactive oxygen species
*redox cycling
2018
Aleksunes Lauren M
Benzoquinones/*metabolism
Cytochrome-B(5) Reductase/*metabolism
Free Radicals/metabolism
Fussell Karma C
Heck Diane E
Humans
Jan Yi-Hua
Kinetics
Laskin Debra L
Laskin Jeffrey D
Liver
Microsomes
Mishin Vladimir
NADP/metabolism
Nitrofurantoin/*metabolism
Oxidation-Reduction
Oxygen Consumption
Reactive Oxygen Species/metabolism
Recombinant Proteins/metabolism
Richardson Jason R
Szilagyi John T
Toxicology and applied pharmacology
Wang Yun
Yang Shaojun
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
1–11
Issue
1
Volume
41
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
HNF4 and COUP-TFII interact to modulate transcription of the cholesterol 7alpha-hydroxylase gene (CYP7A1).
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-01
Subject
The topic of the resource
Humans; Animals; Rats; Liver/metabolism; Recombinant Proteins/metabolism; Base Sequence; Mutation; DNA Primers; Cholesterol 7-alpha-Hydroxylase/*genetics; Transcription Factors/*metabolism; Hepatocyte Nuclear Factor 4; DNA-Binding Proteins/*metabolism; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Luciferases/genetics; COUP Transcription Factor II; COUP Transcription Factors; Phosphoproteins/*metabolism; Genes; Sprague-Dawley; Cultured; Genetic; Tumor Cells; Reporter; Promoter Regions; *Transcription; *Receptors; Steroid
Creator
An entity primarily responsible for making the resource
Stroup D; Chiang J Y
Description
An account of the resource
The gene for cholesterol 7alpha-hydroxylase (CYP7A1) contains a sequence at nt -149 to -118 that was found to play a large role in determining the overall transcriptional activity and regulation of the promoter. Hepatocyte nuclear factor 4 (HNF4) and chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) synergistically activate transcription of the CYP7A1 promoter. Transactivation of CYP7A1 by HNF4 in the human hepatoma cell line, HepG2, was enhanced by cotransfection with COUP-TFII or the basal transcription element binding protein (BTEB). HNF4 prepared from rat liver nuclear extracts bound to oligomers homologous to the nt -146 to -134 sequences in electrophoretic mobility shift assays (EMSA), which corresponded to a conserved region containing a direct repeat of hormone response elements spaced by one nucleotide (DR1). The sequences surrounding this DR1 were found to be essential for the HNF4 transactivation. In vitro-translated COUP-TFII was found to bind the adjacent sequences from nt -139 to -128 (DR0), but COUP-TFII interacted with this region at a much lower affinity than to the COUP-TFII-site at nt -72 to -57 (DR4). Mutations at nt -139 to -128 or nt -72 to -57 reduced the COUP-TFII and HNF4 synergy; however, these
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Receptors
*Transcription
2000
Animals
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
COUP Transcription Factor II
COUP Transcription Factors
Cultured
Department of Integrative Medical Sciences
DNA Primers
DNA-Binding Proteins/*metabolism
Genes
Genetic
Hepatocyte Nuclear Factor 4
Humans
Journal of lipid research
Liver/metabolism
Luciferases/genetics
Mutation
NEOMED College of Medicine
Phosphoproteins/*metabolism
Promoter Regions
Rats
Recombinant Proteins/metabolism
Reporter
Sprague-Dawley
Steroid
Stroup D
Transcription Factors/*metabolism
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1093/toxsci/kfw031" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/toxsci/kfw031</a>
Pages
150–159
Issue
1
Volume
151
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Selective Targeting of Heme Protein in Cytochrome P450 and Nitric Oxide Synthase by Diphenyleneiodonium.
Publisher
An entity responsible for making the resource available
Toxicological sciences : an official journal of the Society of Toxicology
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-05
Subject
The topic of the resource
*cytochrome P450; *flavoenzymes; *heme; *nitric oxide synthase; *reactive oxygen species; Animals; Cytochrome P-450 Enzyme Inhibitors/*pharmacology; Cytochrome P-450 Enzyme System/*metabolism; Dose-Response Relationship; Drug; Heme/*antagonists & inhibitors/metabolism; Humans; Liver/drug effects/enzymology; Mice; Microsomes; Nitric Oxide Synthase Type II/*antagonists & inhibitors/metabolism; Nitric Oxide/metabolism; Onium Compounds/*pharmacology; Rats; Recombinant Proteins/metabolism; Sprague-Dawley; Time Factors
Creator
An entity primarily responsible for making the resource
Szilagyi John T; Mishin Vladimir; Heck Diane E; Jan Yi-Hua; Aleksunes Lauren M; Richardson Jason R; Heindel Ned D; Laskin Debra L; Laskin Jeffrey D
Description
An account of the resource
Cytochrome P450 (CYP) enzymes mediate mixed-function oxidation reactions important in drug metabolism. The aromatic heterocyclic cation, diphenyleneiodonium (DPI), binds flavin in cytochrome P450 reductase and inhibits
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1093/toxsci/kfw031" target="_blank" rel="noreferrer noopener">10.1093/toxsci/kfw031</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*cytochrome P450
*flavoenzymes
*heme
*nitric oxide synthase
*Reactive oxygen species
2016
Aleksunes Lauren M
Animals
Cytochrome P-450 Enzyme Inhibitors/*pharmacology
Cytochrome P-450 Enzyme System/*metabolism
Department of Pharmaceutical Sciences
Dose-Response Relationship
Drug
Heck Diane E
Heindel Ned D
Heme/*antagonists & inhibitors/metabolism
Humans
Jan Yi-Hua
Laskin Debra L
Laskin Jeffrey D
Liver/drug effects/enzymology
Mice
Microsomes
Mishin Vladimir
NEOMED College of Pharmacy
Nitric Oxide Synthase Type II/*antagonists & inhibitors/metabolism
Nitric Oxide/metabolism
Onium Compounds/*pharmacology
Rats
Recombinant Proteins/metabolism
Richardson Jason R
Sprague-Dawley
Szilagyi John T
Time Factors
Toxicological sciences : an official journal of the Society of Toxicology
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M010996200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M010996200</a>
Pages
16040–16044
Issue
19
Volume
276
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Prostate apoptosis response-4 enhances secretion of amyloid beta peptide 1-42 in human neuroblastoma IMR-32 cells by a caspase-dependent pathway.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-05
Subject
The topic of the resource
*Intracellular Signaling Peptides and Proteins; Amyloid beta-Peptides/*biosynthesis/metabolism; Apoptosis Regulatory Proteins; Apoptosis/*physiology; Carrier Proteins/genetics/*physiology; Caspases/*metabolism; Cultured; Enzyme Activation; Humans; Kinetics; Leucine Zippers; Neuroblastoma; Peptide Fragments/*biosynthesis/metabolism; Recombinant Proteins/metabolism; Time Factors; Transfection; Tumor Cells
Creator
An entity primarily responsible for making the resource
Guo Q; Xie J; Chang X; Du H
Description
An account of the resource
Prostate apoptosis response-4 (Par-4) is a leucine zipper protein that promotes neuronal cell death in Alzheimer's disease (AD). Neuronal degeneration in AD may result from extracellular accumulation of amyloid beta peptide (Abeta) 1-42. To examine the effect of Par-4 on Abeta secretion and to reconcile amyloid/apoptosis hypotheses of AD, we generated IMR-32 cell lines that overexpress Par-4 and/or its leucine zipper domain. Overexpression of Par-4 did not significantly affect levels of the endogenously expressed beta amyloid precursor protein but drastically increased the Abeta(1-42)/Abeta(total) ratio in the conditioned media about 6-8 h after trophic factor withdrawal. Time course analysis of caspase activation reveals that Par-4 overexpression exacerbated caspase activation, which is detectable within 2 h after trophic factor withdrawal. Furthermore, inhibition of caspase activity by the broad spectrum caspase inhibitor BD-fmk significantly attenuated the Par-4-induced increase in Abeta 1-42 production. In addition, the effects of Par-4 on secretion of Abeta 1-42 were consistently blocked by co-expression of the leucine zipper domain, indicating that the effect of Par-4 on Abeta secretion may require its interaction with other protein(s). These results suggest that Par-4 increases secretion of Abeta 1-42 largely through a caspase-dependent pathway after apoptotic cascades are initiated.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M010996200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M010996200</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Intracellular Signaling Peptides and Proteins
2001
Amyloid beta-Peptides/*biosynthesis/metabolism
Apoptosis Regulatory Proteins
Apoptosis/*physiology
Carrier Proteins/genetics/*physiology
Caspases/*metabolism
Chang X
Cultured
Du H
Enzyme Activation
Guo Q
Humans
Kinetics
Leucine Zippers
Neuroblastoma
Peptide Fragments/*biosynthesis/metabolism
Recombinant Proteins/metabolism
The Journal of biological chemistry
Time Factors
Transfection
Tumor Cells
Xie J
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1038/emm.2016.78" target="_blank" rel="noreferrer noopener">http://doi.org/10.1038/emm.2016.78</a>
Pages
e257–e257
Issue
9
Volume
48
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Osteoactivin inhibition of osteoclastogenesis is mediated through CD44-ERK signaling.
Publisher
An entity responsible for making the resource available
Experimental & molecular medicine
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-09
Subject
The topic of the resource
*MAP Kinase Signaling System; *Signal Transduction; Animals; Cell Differentiation; Cells; Cultured; Eye Proteins/*metabolism; Hyaluronan Receptors/*metabolism; Inbred C57BL; Male; Membrane Glycoproteins/*metabolism; Mice; Osteoclasts/*cytology/metabolism; RANK Ligand/metabolism; Recombinant Proteins/metabolism
Creator
An entity primarily responsible for making the resource
Sondag Gregory R; Mbimba Thomas S; Moussa Fouad M; Novak Kimberly; Yu Bing; Jaber Fatima A; Abdelmagid Samir M; Geldenhuys Werner J; Safadi Fayez F
Description
An account of the resource
Osteoactivin is a heavily glycosylated protein shown to have a role in bone remodeling. Previous studies from our lab have shown that mutation in Osteoactivin enhances osteoclast differentiation but inhibits their function. To date, a classical receptor and a signaling pathway for Osteoactivin-mediated osteoclast inhibition has not yet been characterized. In this study, we examined the role of Osteoactivin treatment on osteoclastogenesis using bone marrow-derived osteoclast progenitor cells and identify a signaling pathway relating to Osteoactivin function. We reveal that recombinant Osteoactivin treatment inhibited osteoclast differentiation in a dose-dependent manner shown by qPCR, TRAP staining, activity and count. Using several approaches, we show that Osteoactivin binds CD44 in osteoclasts. Furthermore, recombinant Osteoactivin treatment inhibited ERK phosphorylation in a CD44-dependent manner. Finally, we examined the role of Osteoactivin on receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteolysis in vivo. Our data indicate that recombinant Osteoactivin inhibits RANKL-induced osteolysis in vivo and this effect is CD44-dependent. Overall, our data indicate that Osteoactivin is a negative regulator of osteoclastogenesis in vitro and in vivo and that this process is regulated through CD44 and ERK activation.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1038/emm.2016.78" target="_blank" rel="noreferrer noopener">10.1038/emm.2016.78</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*MAP Kinase Signaling System
*Signal Transduction
2016
Abdelmagid Samir M
Animals
Cell Differentiation
Cells
Cultured
Department of Anatomy & Neurobiology
Experimental & molecular medicine
Eye Proteins/*metabolism
Geldenhuys Werner J
Hyaluronan Receptors/*metabolism
Inbred C57BL
Jaber Fatima A
Male
Mbimba Thomas S
Membrane Glycoproteins/*metabolism
Mice
Moussa Fouad M
NEOMED College of Medicine
Novak Kimberly
Osteoclasts/*cytology/metabolism
RANK Ligand/metabolism
Recombinant Proteins/metabolism
Safadi Fayez F
Sondag Gregory R
Yu Bing