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Text
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URL Address
<a href="http://doi.org/10.1186/1465-9921-9-55" target="_blank" rel="noreferrer noopener">http://doi.org/10.1186/1465-9921-9-55</a>
Pages
55–55
Volume
9
Dublin Core
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Title
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RNA interference for CFTR attenuates lung fluid absorption at birth in rats.
Publisher
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Respiratory research
Date
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2008
2008-01
Subject
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Female; Pregnancy; Rats; Genes; Cells; Animal Population Groups; Animal Studies; Lung; Respiratory Mucosa; Genes – Drug Effects; Biological Transport – Drug Effects; Lung – Drug Effects; Lung – Metabolism; Membrane Proteins – Metabolism; Respiratory Mucosa – Drug Effects; Respiratory Mucosa – Metabolism; RNA – Metabolism; RNA – Pharmacodynamics; Water – Metabolism
Creator
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Li T; Koshy S; Folkesson H G
Description
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Background: Small interfering RNA (siRNA) against αENaC (α-subunit of the epithelial Na channel) and CFTR (cystic fibrosis transmembrane conductance regulator) was used to explore ENaC and CTFR function in newborn rat lungs. Methods: Twenty-four hours after trans-thoracic intrapulmonary (ttip) injection of siRNAgenerating plasmid DNA (pSi-0, pSi-4, or pSi-C2), we measured CFTR and ENaC expression, extravascular lung water, and mortality. Results: αENaC and CFTR mRNA and protein decreased by \textasciitilde80% and \textasciitilde85%, respectively, following αENaC and CFTR silencing. Extravascular lung water and mortality increased after αENaC and CFTR-silencing. In pSi-C2-transfected isolated DLE cells there were attenuated CFTR mRNA and protein. In pSi-4-transfected DLE cells αENaC mRNA and protein were both reduced. Interestingly, CFTR-silencing also reduced αENaC mRNA and protein. αENaC silencing, on the other hand, only slightly reduced CFTR mRNA and protein. Conclusion: Thus, ENaC and CFTR are both involved in the fluid secretion to absorption conversion around at birth.
Identifier
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<a href="http://doi.org/10.1186/1465-9921-9-55" target="_blank" rel="noreferrer noopener">10.1186/1465-9921-9-55</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2008
Animal Population Groups
Animal Studies
Biological Transport – Drug Effects
Cells
Female
Folkesson H G
Genes
Genes – Drug Effects
Koshy S
Li T
Lung
Lung – Drug Effects
Lung – Metabolism
Membrane Proteins – Metabolism
Pregnancy
Rats
Respiratory Mucosa
Respiratory Mucosa – Drug Effects
Respiratory Mucosa – Metabolism
Respiratory research
RNA – Metabolism
RNA – Pharmacodynamics
Water – Metabolism