Description
We hypothesized that congenital diaphragmatic hernia (CDH) may decrease distal air space fluid absorption due to immaturity of alveolar epithelial cells from a loss of the normal epithelial Na+ transport, as assessed by amiloride and epithelial Na+ channel (ENaC) and Na-K-ATPase expression, as well as failure to respond to endogenous epinephrine as assessed by propranolol. Timed-pregnant dams were gavage fed 100 mg of nitrofen at 9.5-day gestation to induce CDH in the fetuses, and distal air space fluid absorption experiments were carried out on
Subject
*Gestational Age; *Hernias; Absorption; Amiloride/pharmacology; Animals; Body Fluids/*metabolism; Congenital; Diaphragmatic; Diaphragmatic/*metabolism; Epinephrine/metabolism; Epithelial Sodium Channels; Female; Fetal Organ Maturity; Fetus/*metabolism; Hernia; Male; Newborn; Phenyl Ethers/administration & dosage; Pregnancy; Propranolol/pharmacology; Rats; Respiratory System Abnormalities; Sodium Channels/metabolism; Sodium-Potassium-Exchanging ATPase/metabolism; Sprague-Dawley