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40
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Text
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Pages
13–17
Volume
5
Dublin Core
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Title
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Identification and nucleotide sequence of the leukocyte and reticulocyte forms of rabbit cytochrome b5 mRNA.
Publisher
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SAAS bulletin, biochemistry and biotechnology
Date
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1992
1992-01
Subject
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Animals; Amino Acid Sequence; Rabbits; Base Sequence; Polymerase Chain Reaction; Molecular Sequence Data; Nucleic Acid Hybridization; Cytochromes b5/chemistry/*genetics; Leukocytes/*chemistry; Liver/chemistry; Reticulocytes/*chemistry; RNA; Sequence Homology; Nucleic Acid; Messenger/*chemistry
Creator
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Giordano S J; Steggles A W
Description
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RNA extracted from rabbit leukocytes and reticulocytes was reverse transcribed and used in the Polymerase Chain Reaction technique along with primers designed to amplify the coding sequence of rabbit cytochrome b5. The resultant amplified products were subcloned and analyzed. Sequencing confirmed that leukocyte and liver cDNAs are homologous and encode the membrane-bound form of the protein. In contrast, reticulocytes exhibit a highly similar, but different mRNA which encodes the smaller, soluble cytochrome b5. This is the first example of a cytochrome b5 sequence from a tissue other than liver, erythrocyte or reticulocyte.
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1992
Amino Acid Sequence
Animals
Base Sequence
Cytochromes b5/chemistry/*genetics
Giordano S J
Leukocytes/*chemistry
Liver/chemistry
Messenger/*chemistry
Molecular Sequence Data
Nucleic Acid
Nucleic Acid Hybridization
Polymerase Chain Reaction
Rabbits
Reticulocytes/*chemistry
RNA
SAAS bulletin, biochemistry and biotechnology
Sequence Homology
Steggles A W