1
40
3
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Hyperlink
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URL
https://doi.org/10.3390/cells11203186
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Retinoic Acid Receptor Alpha (RARα) in Macrophages Protects from Diet-Induced Atherosclerosis in Mice
Creator
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Fathima N Cassim Bawa
Raja Gopoju
Yanyong Xu
Shuwei Hu
Yingdong Zhu
Shaoru Chen
Kavita Jadhav
Yanqiao Zhang
Date
A point or period of time associated with an event in the lifecycle of the resource
2022
Description
An account of the resource
Retinoic acid signaling plays an important role in regulating lipid metabolism and inflammation. However, the role of retinoic acid receptor alpha (RARα) in atherosclerosis remains to be determined. In the current study, we investigated the role of macrophage RARα in the development of atherosclerosis. Macrophages isolated from myeloid-specific Rarα-/- (RarαMac-/-) mice showed increased lipid accumulation and inflammation and reduced cholesterol efflux compared to Rarαfl/fl (control) mice. All-trans retinoic acid (AtRA) induced ATP-binding cassette subfamily A member 1 (Abca1) and Abcg1 expression and cholesterol efflux in both RarαMac-/- mice and Rarαfl/fl mice. In Ldlr-/- mice, myeloid ablation of RARα significantly reduced macrophage Abca1 and Abcg1 expression and cholesterol efflux, induced inflammatory genes, and aggravated Western diet-induced atherosclerosis. Our data demonstrate that macrophage RARα protects against atherosclerosis, likely via inducing cholesterol efflux and inhibiting inflammation.
Source
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Cells
. 2022 Oct 11;11(20):3186. doi: 10.3390/cells11203186.
Language
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English
2022
all-trans retinoic acid
Atherosclerosis
cholesterol efflux
Macrophages
Retinoic Acid Receptor alpha
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Text
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URL Address
<a href="http://doi.org/10.1006/abbi.2000.1836" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/abbi.2000.1836</a>
Pages
364–376
Issue
2
Volume
378
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Promoter activity and regulation of the CYP4F2 leukotriene B(4) omega-hydroxylase gene by peroxisomal proliferators and retinoic acid in HepG2 cells.
Publisher
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Archives of biochemistry and biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-06
Subject
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*Gene Expression Regulation; *Promoter Regions; Amino Acid Sequence; Base Sequence; Cell Line; Cloning; Cytochrome P-450 CYP4A; Cytochrome P-450 Enzyme System/*genetics/metabolism; Cytochrome P450 Family 4; Cytoplasmic and Nuclear/metabolism; DNA; DNA Footprinting; Enzymologic; Exons; Genes; Genetic; Genetic/drug effects; Humans; Introns; Leukotriene B4/metabolism; Mixed Function Oxygenases/metabolism; Models; Molecular; Molecular Sequence Data; Peroxisome Proliferators/*metabolism; Receptors; Reporter; Retinoic Acid Receptor alpha; Retinoic Acid/metabolism; Sequence Analysis; Transcription; Transcription Factors/metabolism; Transfection; Tretinoin/*metabolism
Creator
An entity primarily responsible for making the resource
Zhang X; Chen L; Hardwick J P
Description
An account of the resource
The human liver CYP4F2 gene (Accession No. AF221943) encodes a leukotriene B(4) omega-hydroxylase that metabolizes leukotriene B(4) (LTB(4)) to a less potent proinflammatory eicosanoid, 20-OH-LTB(4). We sequenced a 6.7-kb genomic fragment of the human CYP4F2 gene that has the first five exons and 500 bp of the 5'-flanking region. The major transcription start site was found to be 49 bp upstream of the 3' end of exon 1 and the ATG translation initiation codon was located in exon 2. Besides the TATA box at -39 bp and basal transcription factor binding sites, the promoter region and 412-bp intron 1 have several putative binding sites for nuclear factors that may mediate the inflammatory response and lipid homeostasis. We found two DR1 elements in the 5' promoter, a DR2 element in intron 1, and RXR/RAR binding sites in both intron 1 and the 5' promoter. DNase I footprinting revealed three protected sequences, with the region containing two CAATT boxes at -71 and -111 bp important in CYP4F2 gene expression. Luciferase reporter assays showed that the 500-bp upstream sequence has strong promoter activity. Transient transfection experiments identified two sites in the 5' promoter and intron 1 that cooperate in gene transcription while exon 1 and a
Identifier
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<a href="http://doi.org/10.1006/abbi.2000.1836" target="_blank" rel="noreferrer noopener">10.1006/abbi.2000.1836</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
*Promoter Regions
2000
Amino Acid Sequence
Archives of biochemistry and biophysics
Base Sequence
Cell Line
Chen L
Cloning
Cytochrome P-450 CYP4A
Cytochrome P-450 Enzyme System/*genetics/metabolism
Cytochrome P450 Family 4
Cytoplasmic and Nuclear/metabolism
Department of Integrative Medical Sciences
DNA
DNA Footprinting
Enzymologic
Exons
Genes
Genetic
Genetic/drug effects
Hardwick J P
Humans
Introns
Leukotriene B4/metabolism
Mixed Function Oxygenases/metabolism
Models
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Peroxisome Proliferators/*metabolism
Receptors
Reporter
Retinoic Acid Receptor alpha
Retinoic Acid/metabolism
Sequence Analysis
Transcription
Transcription Factors/metabolism
Transfection
Tretinoin/*metabolism
Zhang X
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/hep.26699" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/hep.26699</a>
Pages
1750–1760
Issue
5
Volume
59
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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All-trans-retinoic acid ameliorates hepatic steatosis in mice by a novel transcriptional cascade.
Publisher
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Hepatology (Baltimore, Md.)
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-05
Subject
The topic of the resource
Animals; Basic Helix-Loop-Helix Transcription Factors/genetics; Blood Glucose/analysis; Cytoplasmic and Nuclear/*physiology; Fatty Liver/*drug therapy/metabolism; Gene Expression Regulation; Genetic; Inbred C57BL; Lipid Metabolism; Liver/metabolism; Male; Mice; Non-alcoholic Fatty Liver Disease; PPAR gamma/*genetics; Receptors; Repressor Proteins/genetics; Retinoic Acid Receptor alpha; Retinoic Acid/physiology; Transcription; Tretinoin/pharmacology/*therapeutic use
Creator
An entity primarily responsible for making the resource
Kim Seong-Chul; Kim Chun-Ki; Axe David; Cook Aaron; Lee Mikang; Li Tiangang; Smallwood Nicole; Chiang John Y L; Hardwick James P; Moore David D; Lee Yoon-Kwang
Description
An account of the resource
UNLABELLED: Mice deficient in small heterodimer partner (SHP) are protected from diet-induced hepatic steatosis resulting from increased fatty acid oxidation and decreased lipogenesis. The decreased lipogenesis appears to be a direct consequence of very low expression of peroxisome proliferator-activated receptor gamma 2 (PPAR-gamma2), a potent lipogenic transcription factor, in the SHP(-/-) liver. The current study focused on the identification of a SHP-dependent regulatory cascade that controls PPAR-gamma2 gene expression, thereby regulating hepatic fat accumulation. Illumina BeadChip array (Illumina, Inc., San Diego, CA) and real-time polymerase chain reaction were used to identify genes responsible for the linkage between SHP and PPAR-gamma2 using hepatic RNAs isolated from SHP(-/-) and SHP-overexpressing mice. The initial efforts identify that hairy and enhancer of split 6 (Hes6), a novel transcriptional repressor, is an important mediator of the regulation of PPAR-gamma2 transcription by SHP. The Hes6 promoter is specifically activated by the retinoic acid receptor (RAR) in response to its natural agonist ligand, all-trans retinoic acid (atRA), and is repressed by SHP. Hes6 subsequently represses hepatocyte nuclear factor 4 alpha (HNF-4alpha)-activated PPAR-gamma2 gene expression by direct inhibition of
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/hep.26699" target="_blank" rel="noreferrer noopener">10.1002/hep.26699</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2014
Animals
Axe David
Basic Helix-Loop-Helix Transcription Factors/genetics
Blood Glucose/analysis
Chiang John Y L
Cook Aaron
Cytoplasmic and Nuclear/*physiology
Department of Integrative Medical Sciences
Department of Pharmaceutical Sciences
Fatty Liver/*drug therapy/metabolism
Gene Expression Regulation
Genetic
Hardwick James P
Hepatology (Baltimore, Md.)
Inbred C57BL
Kim Chun-Ki
Kim Seong-Chul
Lee Mikang
Lee Yoon-Kwang
Li Tiangang
Lipid Metabolism
Liver/metabolism
Male
Mice
Moore David D
NEOMED College of Medicine
NEOMED College of Pharmacy
Non-alcoholic Fatty Liver Disease
PPAR gamma/*genetics
Receptors
Repressor Proteins/genetics
Retinoic Acid Receptor alpha
Retinoic Acid/physiology
Smallwood Nicole
Transcription
Tretinoin/pharmacology/*therapeutic use