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Text
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Pages
2419–2432
Issue
11
Volume
36
Dublin Core
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Title
A name given to the resource
Hormonal regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7).
Publisher
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Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-11
Subject
The topic of the resource
Animals; Rats; Gene Expression Regulation; Cell Count; Transfection; Base Sequence; Molecular Sequence Data; Phorbol Esters/pharmacology; Cholesterol 7-alpha-Hydroxylase/*genetics; Luciferases/genetics; Cyclic AMP/pharmacology; Hormones/*pharmacology; Second Messenger Systems/physiology; Cultured; Genetic; Tumor Cells; Cloning; Molecular; *Promoter Regions; *Transcription; Enzymologic/*physiology
Creator
An entity primarily responsible for making the resource
Crestani M; Stroup D; Chiang J Y
Description
An account of the resource
The transcriptional regulation of the rat cholesterol 7 alpha-hydroxylase gene (CYP7) by hormones and signal transduction pathways was studied by transient transfection assay of the promoter activity. HepG2 cells were transfected with deletion mutants of the CYP7 upstream region linked to the luciferase reporter gene. The transcription of CYP7/luciferase chimeric genes was higher in confluent than in subconfluent cultures of HepG2 cells. Glucocorticoid receptors, in the presence of dexamethasone, up-regulated the CYP7 gene through two regions located between -3262 and -2803, and between -344 and -222, respectively. Thyroid hormones did not have any effect on the promoter activity. Insulin inhibited the promoter activity through sequences located between -344 and -222, and abolished the stimulation by dexamethasone. Hence, the insulin effect was dominant over that of glucocorticoids. Treatment of transfected HepG2 cells with phorbol
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
*Transcription
1995
Animals
Base Sequence
Cell Count
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cloning
Crestani M
Cultured
Cyclic AMP/pharmacology
Department of Integrative Medical Sciences
Enzymologic/*physiology
Gene Expression Regulation
Genetic
Hormones/*pharmacology
Journal of lipid research
Luciferases/genetics
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Phorbol Esters/pharmacology
Rats
Second Messenger Systems/physiology
Stroup D
Transfection
Tumor Cells