1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1007/s00011-016-1005-3" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s00011-016-1005-3</a> Pages 187–196 Issue 2 Volume 66 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Demethyleneberberine alleviates inflammatory bowel disease in mice through regulating NF-kappaB signaling and T-helper cell homeostasis. Publisher An entity responsible for making the resource available Inflammation research : official journal of the European Histamine Research Society ... [et al.] Date A point or period of time associated with an event in the lifecycle of the resource 2017 2017-02 Subject The topic of the resource Animals; Anti-Inflammatory Agents/*pharmacology/therapeutic use; Berberine/*analogs & derivatives/pharmacology/therapeutic use; Colitis; Colon/drug effects/immunology/pathology; Cytokines/immunology; Dextran Sulfate; DMB; Female; Helper-Inducer/*drug effects/immunology; Homeostasis/drug effects; Immunoglobulin G/blood; Inbred C57BL; Inflammatory Bowel Diseases/blood/chemically induced/drug therapy/*immunology; Mice; NF-kappa B/*antagonists & inhibitors/immunology; NF-kappaB; RAW 264.7 Cells; Reactive Oxygen Species/metabolism; Signal Transduction/drug effects; Spleen/cytology; T-Lymphocytes; Th cell Creator An entity primarily responsible for making the resource Chen Yingying; Li Rui-Yan; Shi Mei-Jing; Zhao Ya-Xing; Yan Yan; Xu Xin-Xin; Zhang Miao; Zhao Xiao-Tong; Zhang Yu-Bin Description An account of the resource OBJECTIVE: The activation of NF-kappaB signaling and unbalance of T-helper (Th) cells have been reported to play a key role in the pathogenesis of colitis. Cortex Phellodendri Chinensis (CPC) is commonly used to treat inflammation and diarrhea. Demethyleneberberine (DMB), a component of CPC, was reported to treat alcoholic liver disease as a novel natural mitochondria-targeted antioxidant in our previous study. In this study, we investigated whether DMB could protect against dextran sulfate sodium (DSS)-induced inflammatory colitis in mice by regulation of NF-kappaB pathway and Th cells homeostatis. METHODS: Inflammatory colitis mice were induced by 3% DSS, and DMB were orally administered on the doses of 150 and 300 mg/kg. In vitro, DMB (10, 20, 40 muM) and N-acetyl cysteine (NAC, 5 mM) were co-cultured with RAW264.7 for 2 h prior to lipopolysaccharide (LPS) stimulation, and splenocytes from the mice were cultured ex vivo for 48 h for immune response test. RESULTS: In vivo, DMB significantly alleviated the weight loss and diminished myeloperoxidase (MPO) activity, while significantly reduced the production of pro-inflammatory cytokines, such as interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-alpha), and inhibited the activation of Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1007/s00011-016-1005-3" target="_blank" rel="noreferrer noopener">10.1007/s00011-016-1005-3</a> Rights Information about rights held in and over the resource Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). 2017 Animals Anti-Inflammatory Agents/*pharmacology/therapeutic use Berberine/*analogs & derivatives/pharmacology/therapeutic use Chen Yingying Colitis Colon/drug effects/immunology/pathology Cytokines/immunology Dextran Sulfate DMB Female Helper-Inducer/*drug effects/immunology Homeostasis/drug effects Immunoglobulin G/blood Inbred C57BL Inflammation research : official journal of the European Histamine Research Society ... [et al.] Inflammatory Bowel Diseases/blood/chemically induced/drug therapy/*immunology Li Rui-Yan Mice NF-kappa B/*antagonists & inhibitors/immunology NF-kappaB RAW 264.7 Cells Reactive Oxygen Species/metabolism Shi Mei-Jing Signal Transduction/drug effects Spleen/cytology T-Lymphocytes Th cell Xu Xin-Xin Yan Yan Zhang Miao Zhang Yu-Bin Zhao Xiao-Tong Zhao Ya-Xing