1
40
2
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.18632/genesandcancer.62" target="_blank" rel="noreferrer noopener">http://doi.org/10.18632/genesandcancer.62</a>
Pages
220–230
Issue
5
Volume
6
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
CArG-driven GADD45alpha activated by resveratrol inhibits lung cancer cells.
Publisher
An entity responsible for making the resource available
Genes & cancer
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015-05
Subject
The topic of the resource
CArG elements; Egr-1; GADD45a; gene therapy; resveratrol; synthetic promoters
Creator
An entity primarily responsible for making the resource
Shi Qiwen; Geldenhuys Werner; Sutariya Vijaykumar; Bishayee Anupam; Patel Isha; Bhatia Deepak
Description
An account of the resource
We report anticarcinogenic effects of suicide gene therapy that relies on the use of resveratrol-responsive CArG elements from the Egr-1 promoter to induce GADD45alpha. In A549 lung cancer cells, endogenous GADD45alpha was not induced upon resveratrol treatment. Therefore, induction of exogenous GADD45alpha resulted in growth inhibition. Resveratrol transiently induced Egr-1 through ERK/JNK-ElK-1. Hence, we cloned natural or synthetic Egr-1 promoter upstream of GADD45alpha cDNA to create a suicide gene therapy vector. Since natural promoter may have antagonized effects, we tested synthetic promoter that contains either five, six or nine repeats of CArG elements essential in the Egr-1 promoter to drive the expression of GADD45alpha upon resveratrol treatment. Further analysis confirmed that both synthetic promoter and natural Egr-1 promoter were able to "turn on" the expression of GADD45alpha when combined with resveratrol, and subsequently led to suppression of cell proliferation and apoptosis.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.18632/genesandcancer.62" target="_blank" rel="noreferrer noopener">10.18632/genesandcancer.62</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2015
Bhatia Deepak
Bishayee Anupam
CArG elements
Egr-1
GADD45a
Geldenhuys Werner
gene therapy
Genes & cancer
Patel Isha
Resveratrol
Shi Qiwen
Sutariya Vijaykumar
synthetic promoters
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.18632/oncotarget.1995" target="_blank" rel="noreferrer noopener">http://doi.org/10.18632/oncotarget.1995</a>
Pages
3862–3870
Issue
11
Volume
5
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Sequential activation of Elk-1/Egr-1/GADD45alpha by arsenic.
Publisher
An entity responsible for making the resource available
Oncotarget
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-06
Subject
The topic of the resource
Humans; Cell Line; Proto-Oncogene Proteins c-akt/metabolism; MAP Kinase Signaling System; Arsenic/*pharmacology; Bronchi/cytology/drug effects/metabolism; Cell Cycle Proteins/biosynthesis/genetics/*metabolism; Early Growth Response Protein 1/biosynthesis/genetics/*metabolism; Epithelial Cells/drug effects/metabolism; ets-Domain Protein Elk-1/biosynthesis/genetics/*metabolism; Nuclear Proteins/biosynthesis/genetics/*metabolism; RNA; Genetic; Promoter Regions; Messenger/biosynthesis/genetics
Creator
An entity primarily responsible for making the resource
Shi Qiwen; Sutariya Vijaykumar; Bishayee Anupam; Bhatia Deepak
Description
An account of the resource
Long-term exposure to arsenic, an environmental contaminant, leads to increased risks of cancers. In the present study, we investigated the sequential regulation of Elk-1 and Egr-1 on As3+-induced GADD45alpha, an effector of G2/M checkpoint. We found that As3+ transcriptionally induced both Elk-1 and Egr-1, and NF-kappaB binding site was necessary for As3+-induced Egr-1 promoter activity. However, specific inhibition of JNK, ERK, and Elk-1 inhibited Egr-1 induction. Furthermore, silencing of Egr-1 downregulated As3+-induced expression of GADD45alpha and ChIP assay confirmed the direct binding of Egr-1 to GADD45alpha promoter. Taken together, our data indicated that the increase of GADD45alpha in response to As3+ was mediated sequentially by Elk-1 and Egr-1.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.18632/oncotarget.1995" target="_blank" rel="noreferrer noopener">10.18632/oncotarget.1995</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2014
Arsenic/*pharmacology
Bhatia Deepak
Bishayee Anupam
Bronchi/cytology/drug effects/metabolism
Cell Cycle Proteins/biosynthesis/genetics/*metabolism
Cell Line
Early Growth Response Protein 1/biosynthesis/genetics/*metabolism
Epithelial Cells/drug effects/metabolism
ets-Domain Protein Elk-1/biosynthesis/genetics/*metabolism
Genetic
Humans
MAP Kinase Signaling System
Messenger/biosynthesis/genetics
Nuclear Proteins/biosynthesis/genetics/*metabolism
Oncotarget
Promoter Regions
Proto-Oncogene Proteins c-akt/metabolism
RNA
Shi Qiwen
Sutariya Vijaykumar