1
40
4
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jbm.a.30731" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jbm.a.30731</a>
Pages
808–822
Issue
4
Volume
78
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Modified aminosilane substrates to evaluate osteoblast attachment, growth, and gene expression in vitro.
Publisher
An entity responsible for making the resource available
Journal of biomedical materials research. Part A
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-09
Subject
The topic of the resource
*Cell Adhesion; *Cell Division; *Gene Expression; *Silanes/chemistry; Alkaline Phosphatase/metabolism; Animals; Base Sequence; Chick Embryo; Collagen/metabolism; DNA Primers; Osteoblasts/*cytology/enzymology/metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA/isolation & purification/metabolism
Creator
An entity primarily responsible for making the resource
Siperko L M; Jacquet R; Landis W J
Description
An account of the resource
Bone cell-substrate interactions are important to understand in the design, selection, and surface modification of bone implants. To gain insight into such interactions, substrates designed with surface species approximating the physiological environment of bone matrix were studied. Osteoblasts (Ob) grown on three such surfaces were used to evaluate cell-substrate effects on attachment, growth, and gene expression as compared with controls. Initial surface preparation consisted of coating glass slides with aminopropyltriethoxy silane (APTES), after which the coated slides were modified with collagen-rich extracellular matrix components obtained from normally mineralizing avian tendon: the tripeptide arginine-glycine-aspartic acid (arg-gly-asp), or a precipitate formed from a metastable solution containing inorganic ions normally found in blood (simulated body fluid). Each of the modified substrates, as well as the nonmodified (APTES) control, provided distinctly different physical (evidenced by differences in rms roughness) and chemical surfaces for seeding primary osteoblasts obtained from 14-day-old normal embryonic chickens. Cell responses to each of the substrates were evaluated over a 21-day period in terms of Ob growth and growth rate, alkaline phosphatase (ALP) activity, and gene expression of type I collagen (COL I), osteopontin (OPN), osteocalcin (OC), and bone sialoprotein (BSP). From these preliminary experiments, indications are that cell attachment and growth in this study possibly are independent processes, an assumption that compels the need for further studies. Collagen-rich matrix-modified substrates had a distinct advantage over others when cell growth rate, ALP activity, and gene expression were considered; cells on these substrates exhibited increased ALP activity and enhanced expression of BSP, OPN, and OC when compared with those of cells on APTES controls or other modified substrates. These results indicate that matrix-modified substrates such as those used in this study provide favorable templates for tissue generation, suggesting their potential in the design of surfaces for bone implants.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/jbm.a.30731" target="_blank" rel="noreferrer noopener">10.1002/jbm.a.30731</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Cell Adhesion
*Cell Division
*Gene Expression
*Silanes/chemistry
2006
Alkaline Phosphatase/metabolism
Animals
Base Sequence
Chick Embryo
Collagen/metabolism
DNA Primers
Jacquet R
Journal of biomedical materials research. Part A
Landis W J
Osteoblasts/*cytology/enzymology/metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA/isolation & purification/metabolism
Siperko L M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/jsbi.2001.4414" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/jsbi.2001.4414</a>
Pages
313–320
Issue
3
Volume
135
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Aspects of mineral structure in normally calcifying avian tendon.
Publisher
An entity responsible for making the resource available
Journal of structural biology
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-09
Subject
The topic of the resource
Anatomic; Animals; Atomic Force/methods; Calcification; Collagen/chemistry/ultrastructure; Microscopy; Minerals/*chemistry; Models; Molecular; Non-programmatic; Physiologic; Tendons/*chemistry/*ultrastructure; Turkeys
Creator
An entity primarily responsible for making the resource
Siperko L M; Landis W J
Description
An account of the resource
Structural characteristics of normally calcifying leg tendons of the domestic turkey Meleagris gallopavo have been observed for the first time by tapping mode atomic force microscopy (TMAFM), and phase as well as corresponding topographic images were acquired to gain insight into the features of mineralizing collagen fibrils and fibers. Analysis of different regions of the tendon has yielded new information concerning the structural interrelationships in vivo between collagen fibrils and fibers and mineral crystals appearing in the form of plates and plate aggregates. TMAFM images show numerous mineralized collagen structures exhibiting characteristic periodicity (54-70 nm), organized with their respective long axes parallel to each other. In some instances, mineral plates (30-40 nm thick) are found interspersed between and in intimate contact with the mineralized collagen. The edges of such plates lie parallel to the neighboring collagen. Many of these plates appear to be aligned to form larger aggregates (475-600 nm long x 75-90 nm thick) that also retain collagen periodicity along their exposed edges. Intrinsic structural properties of the mineralizing avian tendon have not previously been described on the scale reported in this study. These data provide the first visual evidence supporting the concept that larger plates form from parallel association of smaller ones, and the data fill a gap in knowledge between macromolecular- and anatomic-scale studies of the mineralization of avian tendon and connective tissues in general. The observed organization of mineralized collagen, plates, and plate aggregates maintaining a consistently parallel nature demonstrates the means by which increasing structural complexity may be achieved in a calcified tissue over greater levels of hierarchical order.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/jsbi.2001.4414" target="_blank" rel="noreferrer noopener">10.1006/jsbi.2001.4414</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2001
Anatomic
Animals
Atomic Force/methods
Calcification
Collagen/chemistry/ultrastructure
Journal of structural biology
Landis W J
Microscopy
Minerals/*chemistry
Models
Molecular
Non-programmatic
Physiologic
Siperko L M
Tendons/*chemistry/*ultrastructure
Turkeys
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/japplphysiol.00429.2003" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/japplphysiol.00429.2003</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
2134-2141
Issue
5
Volume
95
Search for Full-text
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Invited review: Role of mechanophysiology in aging of ECM: effects of changes in mechanochemical transduction
Publisher
An entity responsible for making the resource available
Journal of Applied Physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-11
Subject
The topic of the resource
age; apoptosis; collagen; connective tissue; dermal fibroblasts; expansion; extracellular-matrix; gene-expression; growth-factor responsiveness; guinea-pig; human articular chondrocytes; mechanical forces; mechanical strain; phosphorelay system; Physiology; silicone implant; skin; Sport Sciences; tissue
Creator
An entity primarily responsible for making the resource
Silver F H; DeVore D; Siperko L M
Description
An account of the resource
Mechanical forces play a role in the development and evolution of extracellular matrices (ECMs) found in connective tissue. Gravitational forces acting on mammalian tissues increase the net muscle forces required for movement of vertebrates. As body mass increases during development, musculoskeletal tissues and other ECMs are able to adapt their size to meet the increased mechanical requirements. However, the control mechanisms that allow for rapid growth in tissue size during development are altered during maturation and aging. The purpose of this mini-review is to examine the relationship between mechanical loading and cellular events that are associated with downregulation of mechanochemical transduction, which appears to contribute to aging of connective tissue. These changes result from decreases in growth factor and hormone levels, as well as decreased activation of the phosphorelay system that controls cell division, gene expression, and protein synthesis. Studies pertaining to the interactions among mechanical forces, growth factors, hormones, and their receptors will better define the relationship between mechanochemical transduction processes and cellular behavior in aging tissues.
Identifier
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<a href="http://doi.org/10.1152/japplphysiol.00429.2003" target="_blank" rel="noreferrer noopener">10.1152/japplphysiol.00429.2003</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2003
Age
Apoptosis
Collagen
connective tissue
dermal fibroblasts
DeVore D
expansion
extracellular-matrix
gene-expression
growth-factor responsiveness
guinea-pig
human articular chondrocytes
Journal Article
Journal of Applied Physiology
mechanical forces
mechanical strain
phosphorelay system
Physiology
silicone implant
Silver F H
Siperko L M
Skin
Sport Sciences
tissue
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1034/j.1600-0846.2003.00358.x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1034/j.1600-0846.2003.00358.x</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
3-23
Issue
1
Volume
9
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Mechanobiology of force transduction in dermal tissue
Publisher
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Skin Research and Technology
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-02
Subject
The topic of the resource
3-dimensional collagen lattices; age-related-changes; bullous pemphigoid antigen; collagen; Dermatology; epidermal growth-factor; extracellular-matrix; fibrils; gap junctions; guinea-pig skin; human-skin fibroblasts; increases inositol trisphosphate; integrins; mechanical properties; mechanochemical transduction; protein-kinase-c; secondary messengers; skin; smooth-muscle cells
Creator
An entity primarily responsible for making the resource
Silver F H; Siperko L M; Seehra G P
Description
An account of the resource
Background/aims: The influence of mechanical forces on skin has been examined since 1861 when Langer first reported the existence of lines of tension in cadaver skin. Internal tension in the dermis is not only passively transferred to the epidermis but also gives rise to active cell-extracellular matrix and cell-cell mechanical interactions that may be an important part of the homeostatic processes that are involved in normal skin metabolism. The purpose of this review is to analyse how internal and external mechanical loads are applied at the macromolecular and cellular levels in the epidermis and dermis. Methods: A review of the literature suggests that internal and external forces applied to dermal cells appear to be involved in mechanochemical transduction processes involving both cell-cell and cell-extra-cellular matrix (ECM) interactions. Internal forces present in dermis are the result of passive tension that is incorporated into the collagen fiber network during development. Active tension generated by fibroblasts involves specific interactions between cell membrane integrins and macromolecules found in the ECM, especially collagen fibrils. Forces appear to be transduced at the cell-ECM interface via re-arrangement of cytoskeletal elements, activation of stretch-induced changes in ion channels, cell contraction at adherens junctions, activation of cell membrane-associated secondary messenger pathways and through growth factor-like activities that influence cellular proliferation and protein synthesis. Conclusions: Internal and external mechanical loading appears to affect skin biology through mechanochemical transduction processes. Further studies are needed to understand how mechanical forces, energy storage and conversion of mechanical energy into changes in chemical potential of small and large macromolecules may occur and influence the metabolism of dermal cells.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1034/j.1600-0846.2003.00358.x" target="_blank" rel="noreferrer noopener">10.1034/j.1600-0846.2003.00358.x</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2003
3-dimensional collagen lattices
age-related-changes
bullous pemphigoid antigen
Collagen
Dermatology
epidermal growth-factor
extracellular-matrix
fibrils
gap junctions
guinea-pig skin
human-skin fibroblasts
increases inositol trisphosphate
integrins
Journal Article
mechanical properties
mechanochemical transduction
protein-kinase-c
secondary messengers
Seehra G P
Silver F H
Siperko L M
Skin
Skin Research and Technology
smooth-muscle cells