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Tags: Sitkovsky M V

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Purification of recombinant mouse P2X(1) receptor

Tags: 1999, Biochemistry & Molecular Biology, Cell Biology, Chen L P, Faseb Journal, Hardwick J P, Jacobson K A, Journal Article or Conference Abstract Publication, Life Sciences & Biomedicine - Other, Sitkovsky M V, Topics

Purification and recognition of recombinant mouse P2X(1) receptors expressed in a baculovirus system

The hexahistidine-tagged mouse P2X(1) receptor (H-mP2X(1)R), an ATP-gated ion channel receptor, was expressed in a baculovirus system using the pAcHLT-B transfer vector containing a hexahistidine tag. Both widely used denaturing (8M urea) acid…

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