1
40
4
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.cryobiol.2006.10.193" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.cryobiol.2006.10.193</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
36-43
Issue
1
Volume
54
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Cryopreservation Of Porcine Articular Cartilage: Mri And Biochemical Results After Different Freezing Protocols
Publisher
An entity responsible for making the resource available
Cryobiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2007
2007-02
Subject
The topic of the resource
2-step procedure; articular-cartilage; biochemical; cryopreservation; culture cells frozen; diffusion; dimethyl sulfoxide; femoral condyles; fresh; Life Sciences & Biomedicine - Other Topics; magnetic-resonance microscopy; MRI; osteoarticular allografts; osteochondral allograft transplantation; Physiology; porcine; survival; tissue
Creator
An entity primarily responsible for making the resource
Laouar L; Fishbein K; McGann L E; Horton W E; Spencer R G; Jomha N M
Description
An account of the resource
The objective of this study was to investigate the effects of cryopreservation on the components of articular cartilage (AC) matrix by utilizing magnetic resonance imaging (MRI) and biochemical assessments. Porcine AC (10 mm osteochondral dowels) was collected into four groups - (1) phosphate buffered saline (PBS) control, (2) PBS snap frozen in liquid nitrogen, (3) slow-cooled in dimethyl sulfoxide (DMSO), and (4) slow cooled in PBS (in absence of DMSO). MRI results demonstrated three distinct zones in the cartilage. After exposure to ice formation during cryopreservation procedures, alterations in MRI determined matrix fixed charged density and magnetization transfer rate were noted. In addition, biochemical assays demonstrated significant alterations in chondroitin sulfate and hydroxyproline content over time without differences in hydration or DNA content. In conclusion, MRI was able to detect some changes in the intact cartilage matrix structure consistent with biochemical assessments after ice formation during cryopreservation of intact porcine AC. Furthermore, biochemical assessments supported some of these findings and changed significantly after incubating the cartilage matrix for 36-72 h in PBS in terms of chondroitin sulfate and hydroxyproline content. (c) 2006 Elsevier Inc. All rights reserved.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.cryobiol.2006.10.193" target="_blank" rel="noreferrer noopener">10.1016/j.cryobiol.2006.10.193</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2-step procedure
2007
articular-cartilage
biochemical
Cryobiology
cryopreservation
culture cells frozen
diffusion
dimethyl sulfoxide
femoral condyles
Fishbein K
fresh
Horton W E
Jomha N M
Journal Article or Conference Abstract Publication
Laouar L
Life Sciences & Biomedicine - Other Topics
magnetic-resonance microscopy
McGann L E
MRI
osteoarticular allografts
osteochondral allograft transplantation
Physiology
porcine
Spencer R G
Survival
tissue
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1117/1.1922329" target="_blank" rel="noreferrer noopener">http://doi.org/10.1117/1.1922329</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
6-6
Issue
3
Volume
10
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Fourier Transform Infrared Imaging Spectroscopic Analysis Of Tissue Engineered Cartilage: Histologic And Biochemical Correlations
Publisher
An entity responsible for making the resource available
Journal of Biomedical Optics
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-05
Subject
The topic of the resource
Biochemistry & Molecular Biology; bioreactor; chondrocytes; collagen; Fourier transform infrared imaging spectroscopy; hollow fiber bioreactor; hollow-fiber; human articular-cartilage; magnetic-resonance microscopy; matrix; Medical Imaging; model; mri techniques; Nuclear Medicine &; Optics; osteoarthritis; proteoglycans; Radiology; tissue engineered cartilage; tissue engineering
Creator
An entity primarily responsible for making the resource
Kim M; Bi X H; Horton W E; Spencer R G; Camacho N P
Description
An account of the resource
The composition of cartilage is predictive of its in vivo performance. Therefore, the ability to assess its primary macromolecular components, proteoglycan (PG) and collagen, is of great importance. In the current study, we hypothesized that PG content and distribution in tissue engineered cartilage could be determined using Fourier-transform infrared imaging spectroscopy (FT-IRIS). The cartilage was grown from chondrocytes within a hollow fiber bioreactor (HFBR) system previously used extensively to study cartilage development. FT-IRIS analysis showed a gradient of PG content, with the highest content in the center near the nutritive fibers and the lowest near the interior surface of the HFBR. Further, we found significantly greater PG content in the region near culture medium inflow (45.0%) as compared to the outflow region (24.7%) (p < 0.001). This difference paralleled the biochemically determined glycosaminoglycan difference of 42.6% versus 27.8%. In addition, FT-IRIS-determined PG content at specific positions within the tissue sections correlated with histologically determined PG content (R=50.73, p=50.007). In summary, FT-IRIS determination of PG correlates with histological determination of PG and yields quantitatively similar results to biochemical determination of glycosaminoglycan in developing cartilage. (c) 2005 Society of Photo-Optical Instrumentation Engineers.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1117/1.1922329" target="_blank" rel="noreferrer noopener">10.1117/1.1922329</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2005
Bi X H
Biochemistry & Molecular Biology
bioreactor
Camacho N P
Chondrocytes
Collagen
Fourier transform infrared imaging spectroscopy
hollow fiber bioreactor
hollow-fiber
Horton W E
human articular-cartilage
Journal Article or Conference Abstract Publication
Journal of Biomedical Optics
Kim M
magnetic-resonance microscopy
matrix
Medical Imaging
model
mri techniques
Nuclear Medicine &
optics
Osteoarthritis
proteoglycans
Radiology
Spencer R G
tissue engineered cartilage
Tissue Engineering
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.aca.2013.03.053" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.aca.2013.03.053</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
41-49
Volume
779
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Differences In Infrared Spectroscopic Data Of Connective Tissues In Transflectance And Transmittance Modes
Publisher
An entity responsible for making the resource available
Analytica Chimica Acta
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-05
Subject
The topic of the resource
BaF2 window; bone; cartilage; Chemistry; collagen orientation; consequences; Fourier transform infrared imaging spectroscopy; Low-e; proteoglycan; scattering; slide; Transflectance; Transmittance; variance
Creator
An entity primarily responsible for making the resource
Hanifi A; McGoverin C; Ou Y T; Safadi F; Spencer R G; Pleshko N
Description
An account of the resource
Fourier transform infrared imaging spectroscopy (FT-IRIS) has been used extensively to characterize the composition and orientation of macromolecules in thin tissue sections. Earlier and current studies of normal and polarized FT-IRIS data have primarily used tissues sectioned onto infrared transmissive substrates, such as salt windows. Recently, the use of low-emissivity ("low-e") substrates has become of great interest because of their low cost and favorable infrared optical properties. However, data are collected in transflectance mode when using low-e slides and in transmittance mode using salt windows. In the current study we investigated the comparability of these two modes for assessment of the composition of connective tissues. FT-IRIS data were obtained in transflectance and transmittance modes from serial sections of cartilage, bone and tendon, and from a standard polymer, polymethylmethacrylate. Both non-polarized and polarized FTIR data differed in absorbance, and in some cases peak position, between transflectance and transmittance modes. However, the FT-IRIS analysis of the collagen fibril orientation in cartilage resulted in the expected zonal arrangement of fibrils in both transmittance and transflectance. We conclude that numerical comparison of FT-IRIS-derived parameters of tissue composition should account for substrate type and data collection mode, while analysis of overall tissue architecture may be more invariant between modes. (c) 2013 Elsevier B.V. All rights reserved.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.aca.2013.03.053" target="_blank" rel="noreferrer noopener">10.1016/j.aca.2013.03.053</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2013
Analytica Chimica Acta
BaF2 window
Bone
Cartilage
Chemistry
collagen orientation
consequences
Fourier transform infrared imaging spectroscopy
Hanifi A
Journal Article or Conference Abstract Publication
Low-e
McGoverin C
Ou Y T
Pleshko N
proteoglycan
Safadi F
Scattering
slide
Spencer R G
Transflectance
Transmittance
variance
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.joca.2010.01.006" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.joca.2010.01.006</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
724-733
Issue
5
Volume
18
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Modification Of Osteoarthritis In The Guinea Pig With Pulsed Low-intensity Ultrasound Treatment
Publisher
An entity responsible for making the resource available
Osteoarthritis and Cartilage
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
2010-05
Subject
The topic of the resource
aggrecan; articular-cartilage; cartilage; Cartilage repair; degeneration; double-blind trial; electromagnetic-fields; gene-expression; in-vitro; knee osteoarthritis; matrix production; Orthopedics; osteoarthritis; placebo-controlled trials; Rheumatology; therapeutic ultrasound; tissue engineered cartilage; Ultrasound
Creator
An entity primarily responsible for making the resource
Gurkan I; Ranganathan A; Yang X; Horton W E; Todman M; Huckle J; Pleshko N; Spencer R G
Description
An account of the resource
Objective: The Hartley guinea pig develops articular cartilage degeneration similar to that seen in idiopathic human osteoarthritis (OA). We investigated whether the application of pulsed low-intensity ultrasound (PLIUS) to the Hartley guinea pig joint would prevent or attenuate the progression of this degenerative process. Methods: Treatment of male Hartley guinea pigs was initiated at the onset of degeneration (8 weeks of age) to assess the ability of PLIUS to prevent OA, or at a later age (12 months) to assess the degree to which PLIUS acted to attenuate the progression of established disease. PLIUS (30 mW/cm(2)) was applied to stifle joints for 20 min/day over periods ranging from 3 to 10 months, with contralateral limbs serving as controls. Joint cartilage histology was graded according to a modified Mankin scale to evaluate treatment effect. Immunohistochemical staining for interleukin-1 receptor antagonist (IL-1ra), matrix metalloproteinase (MMP)-3, MMP-13, and transforming growth factor (TGF)-beta 1 was performed on the cartilage to evaluate patterns of expression of these proteins. Results: PLIUS did not fully prevent cartilage degeneration in the prevention groups, but diminished the severity of the disease, with the treated joints showing markedly decreased surface irregularities and a much smaller degree of loss of matrix staining as compared to controls. PLIUS also attenuated disease progression in the groups with established disease, although to a somewhat lesser extent as compared to the prevention groups. Immunohistochemical staining demonstrated a markedly decreased degree of TGF-beta 1 production in the PLIUS-treated joints. This indicates less active endogenous repair, consistent with the marked reduction in cartilage degradation. Conclusions: PLIUS exhibits the ability to attenuate the progression of cartilage degeneration in an animal model of idiopathic human OA. The effect was greater in the treatment of early, rather than established, degeneration. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.joca.2010.01.006" target="_blank" rel="noreferrer noopener">10.1016/j.joca.2010.01.006</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2010
aggrecan
articular-cartilage
Cartilage
Cartilage repair
degeneration
double-blind trial
electromagnetic-fields
gene-expression
Gurkan I
Horton W E
Huckle J
in-vitro
Journal Article or Conference Abstract Publication
knee osteoarthritis
matrix production
Orthopedics
Osteoarthritis
Osteoarthritis and cartilage
placebo-controlled trials
Pleshko N
Ranganathan A
Rheumatology
Spencer R G
therapeutic ultrasound
tissue engineered cartilage
Todman M
Ultrasound
Yang X