1
40
3
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Text
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<table width="91" style="border-collapse:collapse;width:68pt;"><colgroup><col width="91" style="width:68pt;" /></colgroup><tbody><tr style="height:15pt;"><td width="91" height="20" class="xl18" style="width:68pt;height:15pt;"><a href="http://doi.org/10.1007/s10162-020-00782-z">http://doi.org/10.1007/s10162-020-00782-z</a></td>
</tr></tbody></table>
Pages
107-126
Volume
22
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Update Year & Number
Jan to Aug list 2021
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Early physiological and cellular indicators of cisplatin-induced ototoxicity.
Creator
An entity primarily responsible for making the resource
Chen Y; Bielefeld EC; Mellott JG; Wang W; Mafi Amir M; Yamoah EN; Bao J
Publisher
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Journal of the Association for Research in Otolaryngology : JARO
Date
A point or period of time associated with an event in the lifecycle of the resource
2021
2021-01-07
Description
An account of the resource
Cisplatin chemotherapy often causes permanent hearing loss, which leads to a multifaceted decrease in quality of life. Identification of early cisplatin-induced cochlear damage would greatly improve clinical diagnosis and provide potential drug targets to prevent cisplatin’s ototoxicity. With improved functional and immunocytochemical assays, a recent seminal discovery revealed that synaptic loss between inner hair cells and spiral ganglion neurons is a major form of early cochlear damage induced by noise exposure or aging. This breakthrough discovery prompted the current study to determine early functional, cellular, and molecular changes for cisplatin-induced hearing loss, in part to determine if synapse injury is caused by cisplatin exposure. Cisplatin was delivered in one to three treatment cycles to both male and female mice. After the cisplatin treatment of three cycles, threshold shift was observed across frequencies tested like previous studies. After the treatment of two cycles, beside loss of outer hair cells and an increase in high-frequency hearing thresholds, a significant latency delay of auditory brainstem response wave 1 was observed, including at a frequency region where there were no changes in hearing thresholds. The wave 1 latency delay was detected as early cisplatin-induced ototoxicity after only one cycle of treatment, in which no significant threshold shift was found. In the same mice, mitochondrial loss in the base of the cochlea and declining mitochondrial morphometric health were observed. Thus, we have identified early spiral ganglion-associated functional and cellular changes after cisplatin treatment that precede significant threshold shift.
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<table width="91" style="border-collapse:collapse;width:68pt;"><colgroup><col width="91" style="width:68pt;" /></colgroup><tbody><tr style="height:15pt;"><td width="91" height="20" class="xl18" style="width:68pt;height:15pt;"><a href="http://doi.org/10.1007/s10162-020-00782-z">http://doi.org/10.1007/s10162-020-00782-z</a></td>
</tr></tbody></table>
Format
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Journal Article
2021
cisplatin
Hair Cells
Ototoxicity
Schwann cell
Spiral ganglion neuron
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s10162-020-00782-z" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s10162-020-00782-z</a>
ISSN
1438-7573
Search for Full-text
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<a href="http://neomed.idm.oclc.org/login?url=http://doi.org/10.1007/s10162-020-00782-z" target="_blank" rel="noreferrer noopener">NEOMED Full-text Holding (if available) - Proxy DOI: 10.1007/s10162-020-00782-z</a>
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Update Year & Number
January 2021 List
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Early physiological and cellular indicators of cisplatin-induced ototoxicity.
Publisher
An entity responsible for making the resource available
Journal of the Association for Research in Otolaryngology : JARO
Date
A point or period of time associated with an event in the lifecycle of the resource
2021
2021-01-07
Subject
The topic of the resource
cisplatin; hair cells; ototoxicity; Schwann cell; spiral ganglion neuron
Creator
An entity primarily responsible for making the resource
Chen Y; Bielefeld EC; Mellott JG; Wang W; Mafi Amir M; Yamoah EN; Bao J
Description
An account of the resource
Cisplatin chemotherapy often causes permanent hearing loss, which leads to a multifaceted decrease in quality of life. Identification of early cisplatin-induced cochlear damage would greatly improve clinical diagnosis and provide potential drug targets to prevent cisplatin's ototoxicity. With improved functional and immunocytochemical assays, a recent seminal discovery revealed that synaptic loss between inner hair cells and spiral ganglion neurons is a major form of early cochlear damage induced by noise exposure or aging. This breakthrough discovery prompted the current study to determine early functional, cellular, and molecular changes for cisplatin-induced hearing loss, in part to determine if synapse injury is caused by cisplatin exposure. Cisplatin was delivered in one to three treatment cycles to both male and female mice. After the cisplatin treatment of three cycles, threshold shift was observed across frequencies tested like previous studies. After the treatment of two cycles, beside loss of outer hair cells and an increase in high-frequency hearing thresholds, a significant latency delay of auditory brainstem response wave 1 was observed, including at a frequency region where there were no changes in hearing thresholds. The wave 1 latency delay was detected as early cisplatin-induced ototoxicity after only one cycle of treatment, in which no significant threshold shift was found. In the same mice, mitochondrial loss in the base of the cochlea and declining mitochondrial morphometric health were observed. Thus, we have identified early spiral ganglion-associated functional and cellular changes after cisplatin treatment that precede significant threshold shift.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/s10162-020-00782-z" target="_blank" rel="noreferrer noopener">10.1007/s10162-020-00782-z</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Format
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journalArticle
2021
Bao J
Bielefeld EC
Chen Y
cisplatin
Department of Anatomy & Neurobiology
Hair Cells
January 2021 List
Journal of the Association for Research in Otolaryngology : JARO
journalArticle
Mafi Amir M
Mellott JG
NEOMED College of Medicine
Ototoxicity
Schwann cell
Spiral ganglion neuron
Wang W
Yamoah EN
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.heares.2018.07.008" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.heares.2018.07.008</a>
Pages
88–96
Volume
367
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Detection of single mRNAs in individual cells of the auditory system.
Publisher
An entity responsible for making the resource available
Hearing research
Date
A point or period of time associated with an event in the lifecycle of the resource
2018
2018-09
Subject
The topic of the resource
Cochlea; Immunohistochemistry; Inner hair cell; Outer hair cell; Single-molecule fluorescence in situ hybridization; Spiral ganglion neuron
Creator
An entity primarily responsible for making the resource
Salehi Pezhman; Nelson Charlie N; Chen Yingying; Lei Debin; Crish Samuel D; Nelson Jovitha; Zuo Hongyan; Bao Jianxin
Description
An account of the resource
Gene expression analysis is essential for understanding the rich repertoire of cellular functions. With the development of sensitive molecular tools such as single-cell RNA sequencing, extensive gene expression data can be obtained and analyzed from various tissues. Single-molecule fluorescence in situ hybridization (smFISH) has emerged as a powerful complementary tool for single-cell genomics studies because of its ability to map and quantify the spatial distributions of single mRNAs at the subcellular level in their native tissue. Here, we present a detailed method to study the copy numbers and spatial localizations of single mRNAs in the cochlea and inferior colliculus. First, we demonstrate that smFISH can be performed successfully in adult cochlear tissue after decalcification. Second, we show that the smFISH signals can be detected with high specificity. Third, we adapt an automated transcript analysis pipeline to quantify and identify single mRNAs in a cell-specific manner. Lastly, we show that our method can be used to study possible correlations between transcriptional and translational activities of single genes. Thus, we have developed a detailed smFISH protocol that can be used to study the expression of single mRNAs in specific cell types of the peripheral and central auditory systems.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.heares.2018.07.008" target="_blank" rel="noreferrer noopener">10.1016/j.heares.2018.07.008</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2018
Bao Jianxin
Chen Yingying
Cochlea
Crish Samuel D
Department of Anatomy & Neurobiology
Department of Pharmaceutical Sciences
Hearing research
Immunohistochemistry
Inner hair cell
Lei Debin
Nelson Charlie N
Nelson Jovitha
NEOMED College of Medicine
NEOMED College of Pharmacy
Outer hair cell
Salehi Pezhman
Single-molecule fluorescence in situ hybridization
Spiral ganglion neuron
Zuo Hongyan