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Text
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URL Address
<a href="http://doi.org/10.1016/j.scr.2016.05.009" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.scr.2016.05.009</a>
Pages
54–61
Issue
1
Volume
17
Dublin Core
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Title
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Methods and insights from the characterization of osteoprogenitor cells of bats (Mammalia: Chiroptera).
Publisher
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Stem cell research
Date
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2016
2016-07
Subject
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Animals; Bone Marrow Cells/cytology; Cell Differentiation/genetics; Cell Proliferation; Cells; Cellular Reprogramming; Chiroptera; Core Binding Factor Alpha 1 Subunit/genetics/metabolism; Cultured; Inbred C57BL; Mice; Osteoblasts/cytology/*metabolism; Osteocalcin/genetics/metabolism; Osteogenesis/*genetics; Real-Time Polymerase Chain Reaction; Stem Cells/*cytology/metabolism; Transcription Factors/genetics/metabolism
Creator
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Ball H C; Moussa F M; Mbimba T; Orman R; Safadi F F; Cooper L N
Description
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Osteoprogenitor cells contribute to the development and maintenance of skeletal tissues. Bats are unique model taxa whose cellular processes are poorly understood, especially in regards to skeletal biology. Forelimb bones of bats, unlike those of terrestrial mammals, bend during flight and function in controlled deformation. As a first step towards understanding the molecular processes governing deposition of this flexible bone matrix, we provide the first method for isolation and differentiation of cell populations derived from the bone marrow and cortical bone of bats, and compare results with those harvested from C57BL/6J mice. Osteogenic capacity of these cells was assessed via absolute quantitative real-time PCR (qPCR) and through quantification of in vitro mineral deposition. Results indicate the differentiated bone cells of bats display significantly lower gene expression of known osteogenic markers (Runt-related transcription factor (RUNX2), osteocalcin (BGLAP) and osterix (SP7)), and deposit a less-mineralized matrix compared with murine controls. By characterizing the in vitro performance of osteoprogenitor cells throughout differentiation and matrix production, this study lays the ground work for in vitro manipulations of bat stem and osteoprogenitor cells and extends our understanding of the cellular diversity across mammals that occupy different habitats.
Identifier
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<a href="http://doi.org/10.1016/j.scr.2016.05.009" target="_blank" rel="noreferrer noopener">10.1016/j.scr.2016.05.009</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2016
Animals
Ball H C
Bone Marrow Cells/cytology
Cell Differentiation/genetics
Cell Proliferation
Cells
Cellular Reprogramming
Chiroptera
Cooper L N
Core Binding Factor Alpha 1 Subunit/genetics/metabolism
Cultured
Department of Anatomy & Neurobiology
Inbred C57BL
Mbimba T
Mice
Moussa F M
NEOMED College of Medicine
Orman R
Osteoblasts/cytology/*metabolism
Osteocalcin/genetics/metabolism
Osteogenesis/*genetics
Real-Time Polymerase Chain Reaction
Safadi F F
Stem cell research
Stem Cells/*cytology/metabolism
Transcription Factors/genetics/metabolism