1
40
34
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Text
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<a href="http://doi.org/10.1002/hep.31604" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/hep.31604</a>
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ISSN
1527-3350 0270-9139
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<a href="http://neomed.idm.oclc.org/login?url=http://doi.org/10.1002/hep.31604" target="_blank" rel="noreferrer noopener">NEOMED Full-text Holding (if available) - Proxy DOI: 10.1002/hep.31604</a>
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Update Year & Number
October 2020 List
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Integrative Medical Sciences
NEOMED Postdoc Publications
Dublin Core
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Title
A name given to the resource
Hepatocyte nuclear factor 4α prevents the steatosis-to-NASH progression by regulating p53 and bile acid signaling.
Publisher
An entity responsible for making the resource available
Hepatology
Date
A point or period of time associated with an event in the lifecycle of the resource
2020
2020-10-23
Subject
The topic of the resource
steatohepatitis; apoptosis; bile acid; HNF4α; lipolysis; P53
Creator
An entity primarily responsible for making the resource
Xu Y;Zhu Y;Hu S;Xu Y;Stroup D;Pan X;Bawa FC;Chen S;Gopoju R;Yin Liya;Zhang Y
Description
An account of the resource
Hepatocyte nuclear factor 4α (HNF4α) is highly enriched in the liver, but its role in the progression of liver steatosis (NAFL) to non-alcoholic steatohepatitis (NASH) has not been elucidated. In this study, we investigated the effect of gain or loss of hepatocyte HNF4α function on the development and progression of non-alcoholic fatty liver disease (NAFLD) in mice. Over-expression of human HNF4α protected against high fat/cholesterol/fructose (HFCF) diet-induced steatohepatitis whereas loss of hepatocyte Hnf4α had opposite effects. HNF4α prevented hepatic triglyceride accumulation by promoting hepatic triglyceride lipolysis, fatty acid oxidation and VLDL secretion. Furthermore, HNF4α suppressed the progression of NAFL to NASH. Over-expression of human HNF4α inhibited HFCF diet-induced steatohepatitis in control mice but not in hepatocyte-specific p53(-/-) mice. In HFCF diet-fed mice lacking hepatic Hnf4α, recapitulation of hepatic expression of HNF4α targets cholesterol 7α-hydroxylase and sterol 12α-hydroxylase normalized hepatic triglyceride levels and attenuated steatohepatitis. CONCLUSIONS: The current study indicates that hepatocyte HNF4α protects against diet-induced development and progression of NAFLD by coordinating the regulation of lipolytic, p53 and bile acid signaling pathways. Targeting hepatic HNF4α may be useful for treatment of NASH.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/hep.31604" target="_blank" rel="noreferrer noopener">10.1002/hep.31604</a>
Format
The file format, physical medium, or dimensions of the resource
journalArticle
2020
Apoptosis
Bawa FC
bile acid
Chen S
Department of Integrative Medical Sciences
Gopoju R
Hepatology
HNF4α
Hu S
journalArticle
Lipolysis
NEOMED College of Medicine
NEOMED College of Medicine Postdoc
NEOMED Postdoc Publications
October 2020 List
p53
Pan X
steatohepatitis
Stroup D
Xu Y
Yin Liya
Zhang Y
Zhu Y
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
2865-2865
Issue
6
Volume
10
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Title
A name given to the resource
Transcriptional Regulation Of The Human Cholesterol 7 Alpha-hydroxylase Gene (cyp7a) In Transiently And Stably Transfected Hepg2 Cells
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-04
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Marrapodi M; Wang D P; Stroup D; Chiang J Y L
Identifier
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n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1996
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Faseb Journal
Life Sciences & Biomedicine - Other
Marrapodi M
Stroup D
Topics
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A1204-A1204
Issue
9
Volume
11
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Title
A name given to the resource
Identification of a retinoic acid response element in the rat cholesterol 7 alpha-hydroxylase gene (CYP7A)
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
1997-07
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Crestani M; Stroup D; Baffi G; Galli G; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1997
Baffi G
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Crestani M
Faseb Journal
Galli G
Journal Article or Conference Abstract Publication
Life Sciences & Biomedicine - Other
Stroup D
Topics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
2192-2200
Issue
11
Volume
39
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Title
A name given to the resource
Transcriptional activation of the cholesterol 7 alpha-hydroxylase gene (CYP7A) by nuclear hormone receptors
Publisher
An entity responsible for making the resource available
Journal of Lipid Research
Date
A point or period of time associated with an event in the lifecycle of the resource
1998
1998-11
Subject
The topic of the resource
rat; liver; bile acid synthesis; Biochemistry & Molecular Biology; expression; messenger-rna; elements; metabolism; promoter; nuclear; mutations; cytochrome P450; cholesterol 7; alpha-hydroxylase; bile acid response element; factor coup-tf; gene transcription and regulation; hormone receptor; retinoic acid receptors
Creator
An entity primarily responsible for making the resource
Crestani M; Sadeghpour A; Stroup D; Galli G; Chiang J Y L
Description
An account of the resource
The gene encoding cholesterol 7 alpha-hydroxylase (CYP7A), the rate-limiting enzyme in bile acid synthesis, is transcriptionally regulated by bile acids and hormones. Previously, we have identified two bile acid response elements (BARE) in the promoter of the CYP7A gene, The BARE II is located in nt -149/-118 region and contains three hormone response element (HRE)-like sequences that form two overlapping nuclear receptor binding sites. One is a direct repeat separated by one nucleotide DR1 (-146-TGGACTtAGTTCA-134) and the other is a direct repeat separated by five nucleotides DR5 (-139-AGTTCAaggccGGG TAA-123). Mutagenesis of these HRE sequences resulted in lower transcriptional activity of the CYP7A promoter/reporter genes in transient transfection assay in HepG2 cells. The orphan nuclear receptor, hepatocyte nuclear factor 4 (HNF-4)(1), binds to the DR1 sequence as assessed by electrophoretic mobility shift assay, and activates the CYP7A promoter/reporter activity by about 9-fold. Cotransfection of HNF-4 plasmid with another orphan nuclear receptor, chicken ovalbumin upstream promoter-transcription factor LI (COUP-TRI), synergistically activated the CYP7A transcription by 80-fold. The DR5 binds the RXR/RAR heterodimer, A hepatocyte nuclear factor-3 (HNF-3) binding site (-175-TGTTTGTTCT-166) was identified. HNF-3 was required for both basal transcriptional activity and stimulation of the rat CYP7A promoter activity by retinoic acid, Combinatorial interactions and binding of these transcription factors to BAREs may modulate the promoter activity and also mediate bile acid repression of CYP7A gene transcription.
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1998
alpha-hydroxylase
bile acid response element
Bile acid synthesis
Biochemistry & Molecular Biology
Chiang J Y L
cholesterol 7
Crestani M
cytochrome P450
elements
expression
factor coup-tf
Galli G
gene transcription and regulation
hormone receptor
Journal Article or Conference Abstract Publication
Journal of lipid research
Liver
messenger-rna
Metabolism
mutations
nuclear
promoter
rat
retinoic acid receptors
Sadeghpour A
Stroup D
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A1383-A1383
Issue
7
Volume
8
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Title
A name given to the resource
EFFECT OF BILE-ACIDS AND STEROID THYROID-HORMONES ON THE CHOLESTEROL 7-ALPHA-HYDROXYLASE GENE (CYP7) IN HEPG2 CELLS
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-04
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Crestani M; Karam W G; Stroup D; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1994
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Crestani M
Faseb Journal
Journal Article or Conference Abstract Publication
Karam W G
Life Sciences & Biomedicine - Other
Stroup D
Topics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Volume
80
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Title
A name given to the resource
Molecular mechanism of regulation of cholesterol 7 alpha-hydroxylase gene
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Stroup D; Crestani M; Wang D P
Identifier
An unambiguous reference to the resource within a given context
n/a
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The file format, physical medium, or dimensions of the resource
Book/Monograph
1995
Book/Monograph
Chiang J Y L
Crestani M
Stroup D
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Volume
108
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Title
A name given to the resource
Transcriptional regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7A) by nuclear hormone receptors bound to the bile acid response elements (BARE)
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
1999
Subject
The topic of the resource
activation; identification; promoter
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Stroup D; Crestani M; Sadeghpour A
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Book/Monograph
1999
activation
Book/Monograph
Chiang J Y L
Crestani M
identification
promoter
Sadeghpour A
Stroup D
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A543-A543
Issue
4
Volume
8
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Title
A name given to the resource
IDENTIFICATION OF A LIVER NUCLEAR-PROTEIN FACTOR-RESPONSIVE TO THE REPRESSION OF CHOLESTEROL 7-ALPHA-HYDROXYLASE GENE BY BILE-ACIDS
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-03
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Stroup D
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1994
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Faseb Journal
Journal Article or Conference Abstract Publication
Life Sciences & Biomedicine - Other
Stroup D
Topics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.275.15.10918" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.275.15.10918</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
10918-10924
Issue
15
Volume
275
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Title
A name given to the resource
Farnesoid X receptor responds to bile acids and represses cholesterol 7 alpha-hydroxylase gene (CYP7A1) transcription
Publisher
An entity responsible for making the resource available
Journal of Biological Chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-04
Subject
The topic of the resource
orphan nuclear receptor; Biochemistry & Molecular Biology; expression; pathway; activation; identification; promoter; element; metabolites; hepg2 cells; ligands
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Kimmel R; Weinberger C; Stroup D
Description
An account of the resource
Cholesterol 7 alpha-hydroxylase gene (CYP7A1) transcription is repressed by bile acids. The goal of this study is to elucidate the mechanism of CYP7A1 transcription by bile acid-activated farnesoid X receptor (FXR) in its native promoter and cellular context and to identify FXR response elements in the gene. In Chinese hamster ovary cells transfected with retinoid X receptor alpha (RXR alpha)/FXR, only chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA) were able to stimulate a heterologous promoter/reporter containing an ecdysone response element. In HepG2 cells, all bile acids (25 mu M) were able to repress CYP7A1/luciferase reporter activity, and only CDCA and DCA further repressed reporter activity when cotransfected with RXR alpha/FXR, The concentration of CDCA required to inhibit 50% of reporter activity (IC(50)) was determined to be approximately 25 mu M without FXR and 10 mu M with FXR. Deletion analysis revealed that the bile acid response element located between nucleotides -148 and -128 was the FXR response element, but RXR alpha/FXR did not bind to this sequence. These results suggest that bile acid-activated FXR exerts its inhibitory effect on CYP7A1 transcription by an indirect mechanism, in contrast to the stimulation and binding of FXR to intestinal bile acid-binding protein gene promoter. Results also reveal that bile acid receptors other than FXR are present in HepG2 cells.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.275.15.10918" target="_blank" rel="noreferrer noopener">10.1074/jbc.275.15.10918</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2000
activation
Biochemistry & Molecular Biology
Chiang J Y L
element
expression
hepg2 cells
identification
Journal Article or Conference Abstract Publication
Journal of Biological Chemistry
Kimmel R
Ligands
metabolites
orphan nuclear receptor
pathway
promoter
Stroup D
Weinberger C
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0378-1119(00)00518-7" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0378-1119(00)00518-7</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
257-265
Issue
1
Volume
262
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Title
A name given to the resource
Regulation of cholesterol 7 alpha-hydroxylase gene (CYP7A1) transcription by the liver orphan receptor (LXR alpha)
Publisher
An entity responsible for making the resource available
Gene
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-01
Subject
The topic of the resource
bile acid synthesis; expression; Signaling; dietary-cholesterol; Bile acids; pathway; nuclear receptor; nuclear receptors; promoter; Genetics & Heredity; x-receptor; cytochrome P450; gene regulation; reverse cholesterol transport; hepg2 cells; coup-tfii; ligands
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Kimmel R; Stroup D
Description
An account of the resource
The cholesterol 7 alpha -hydroxylase gene (CYP7A1) plays an important role in regulation of bile acid biosynthesis and cholesterol homeostasis. Oxysterol receptor, LXR, stimulates, whereas the bile acid receptor, FXR, inhibits CYP7A1 transcription. The goal of this study was to investigate the role of LXR alpha on the regulation of rat, human and hamster CYP7A1 transcription in its native promoter and cellular context. Cotransfection with LXR alpha and RXR alpha expression plasmids strongly stimulated rat CYP7A1/luciferase reporter activity in HepG2 cells and oxysterol was not required. However, LXR alpha had much less effect on hamster and no significant effect on human CYP7A1 promoter activity in HepG2 cells. In Chinese hamster ovary cells, cotransfection with LXR alpha stimulated reporter activity by less than 2-fold and addition of 22(R)-hydroxycholesterol caused a small but significant stimulation of rat, human and hamster CYP7A1 promoter activity. At least two direct repeats of AGGTCA-like sequences with 4-base spacing (DR4) and five-base spacing (DR5), in previously identified bile acid response elements of the rat CYP7A1 were able to bind LXR alpha /RXR alpha and confer LXR alpha stimulation. However, LXR alpha did not bind to the corresponding sequences of the human gene and bound weakly to hamster and mouse DR4 sequences. Therefore, rats and mice have the unusual capacity to convert cholesterol to bile acids by LXR alpha -mediated stimulation of CYP7A1 transcription, whereas other species do not respond to cholesterol and develop hypercholesterolemia on a diet high in cholesterol. (C) 2001 Elsevier Science B.V. All rights reserved.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0378-1119(00)00518-7" target="_blank" rel="noreferrer noopener">10.1016/s0378-1119(00)00518-7</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2001
Bile acid synthesis
BILE acids
Chiang J Y L
coup-tfii
cytochrome P450
dietary-cholesterol
expression
gene
Gene Regulation
Genetics & Heredity
hepg2 cells
Journal Article or Conference Abstract Publication
Kimmel R
Ligands
Nuclear Receptor
Nuclear Receptors
pathway
promoter
reverse cholesterol transport
Signaling
Stroup D
x-receptor
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
2866-2866
Issue
6
Volume
10
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional regulation of the rat cholesterol 7 alpha-hydroxylase gene (CYP7A) by bile acids
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-04
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Crestani M; Stroup D
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1996
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Crestani M
Faseb Journal
Journal Article or Conference Abstract Publication
Life Sciences & Biomedicine - Other
Stroup D
Topics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
319A-319A
Issue
4
Volume
30
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Farnesoid X receptor (FXR) is a bile acid receptor that mediates transcriptional regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7A1) by bile acids
Publisher
An entity responsible for making the resource available
Hepatology
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
1999-10
Subject
The topic of the resource
Gastroenterology & Hepatology
Creator
An entity primarily responsible for making the resource
Chiang J Y; Kimmel R; Weinberger C; Stroup D
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1999
Chiang J Y
Gastroenterology & Hepatology
Hepatology
Journal Article or Conference Abstract Publication
Kimmel R
Stroup D
Weinberger C
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0016-5085(00)86175-2" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0016-5085(00)86175-2</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A1006-A1006
Issue
4
Volume
118
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bile acids and FXR represses cholesterol 7A-hydroxylase (CYP7A1), sterol 12A-hydroxylase (CYP8B1) and sterol 27-hydroxylase (CYP27A1), but not oxysterol 7A-hydroxylase (CYP7B1) gene transcription
Publisher
An entity responsible for making the resource available
Gastroenterology
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-04
Subject
The topic of the resource
Gastroenterology & Hepatology
Creator
An entity primarily responsible for making the resource
Chiang J Y; Chen W; Zheng M; Wu Z; Kimmel R; Stroup D
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0016-5085(00)86175-2" target="_blank" rel="noreferrer noopener">10.1016/s0016-5085(00)86175-2</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2000
Chen W
Chiang J Y
Gastroenterology
Gastroenterology & Hepatology
Journal Article or Conference Abstract Publication
Kimmel R
Stroup D
Wu Z
Zheng M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1402-1412
Issue
9
Volume
42
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Nuclear receptor-mediated repression of human cholesterol 7 alpha-hydroxylase gene transcription by bile acids
Publisher
An entity responsible for making the resource available
Journal of Lipid Research
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-09
Subject
The topic of the resource
liver; bile acid synthesis; Biochemistry & Molecular Biology; expression; messenger-rna; activation; identification; promoter; cytochrome P450; shp; cyp7a; hepg2 cells; mechanism of gene regulation; orphan receptor
Creator
An entity primarily responsible for making the resource
Chen W L; Owsley E; Yang Y Z; Stroup D; Chiang J Y L
Description
An account of the resource
Hydrophobic bile acids strongly repressed transcription of the human cholesterol 7 alpha -hydroxylase gene (CYP7A1) in the bile acid biosynthetic pathway in the Ever. Farnesoid X receptor (FXR) repressed CYP7A1/Luc reporter activity in a transfection assay in human liver-derived HepG2 cells, but not in human embryonic kidney (HEK) 293 cells. FXR-binding activity was required for bile acid repression of CYP7A1 transcription despite the fact that FXR did not bind to the CYP7A1 promoter. FXR-induced liver-specific factors must be required for mediating bile acid repression. Bile acids and FXR repressed endogenous CYP7A1 but stimulated a-fetoprotein transcription factor (FTF) and small heterodimer partner (SHP) mRNA expression in HepG2 cells. Feeding of rats with chenodeoxycholic acid repressed CYP7A1, induced FIT, but had no effect on SHP mRNA expression in the liver. FIT strongly repressed CYP7A1 transcription in a dose-dependent manner, and SHP further inhibited CYP7A1 in HepG2 cells, but not in HEK 293 cells. FXR only moderately stimulated SHP transcription, whereas FIT strongly inhibited SHP transcription in HepG2 cells. Results revealed that FTF was a dominant negative factor that was induced by bile acid-activated FXR to inhibit both CYP7A1 and SHP transcription. Differential regulation of FTF and SHP expression by bile acids may explain the wide variation in CYP7A1 expression and the rate of bile acid synthesis and regulation in different species.
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2001
activation
Bile acid synthesis
Biochemistry & Molecular Biology
Chen W L
Chiang J Y L
cyp7a
cytochrome P450
expression
hepg2 cells
identification
Journal Article or Conference Abstract Publication
Journal of lipid research
Liver
mechanism of gene regulation
messenger-rna
orphan receptor
Owsley E
promoter
SHP
Stroup D
Yang Y Z
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1093/nar/27.10.2219" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/nar/27.10.2219</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
2219-2226
Issue
10
Volume
27
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Oxidative damage of DNA by chromium(V) complexes: relative importance of base versus sugar oxidation
Publisher
An entity responsible for making the resource available
Nucleic Acids Research
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
1999-05
Subject
The topic of the resource
Biochemistry & Molecular Biology; in-vitro; nucleic-acids; abstraction; aqueous-solution; carcinogen chromium(vi); hydrogen; molecular-oxygen; radical formation; redox potentials; singlet oxygen; strand breaks
Creator
An entity primarily responsible for making the resource
Bose R N; Moghaddas S; Mazzer P A; Dudones L P; Joudah L; Stroup D
Description
An account of the resource
Chromium(V)-mediated oxidative damage of deoxyribonucleic acids was investigated at neutral pH in aqueous solution by utilizing bis(2-ethyl-2-hydroxybutanato)oxochromate(V) (I) and bis(hydroxyethyl)amino-tris(hydroxymethyl)methane)oxochromate(V) (II). Single-stranded and double-stranded (ds) calf thymus and human placenta DNA, as well as two oligomers, 5'-GATCTAGTAGGAGGACAAATAGTGTPTG-3' and 5'-GATCCAAGCAAACACTATTTGTCCTCCTACTA-3', were reacted with the chromium(V) complexes. Most products were separated and characterized by chromatographic and spectroscopic methods, Polyacrylamide gel electrophoresis experiments reveal more damage at G sites in comparison to other bases. Three primary oxidation products, 5-methylene-2-furanone (5-MF), furfural and 8-oxo-2'-deoxyguanosine, were characterized, A minor product, which appears to be thymine propenal, was also observed. The dsDNA produces more furfural than furanone, The formation of these two products resulted from hydrogen abstraction dr hydride transfer from C1' and C5' positions of the ribose to the oxo-chromium(V) center. Since no enhancements of these products (except propenal) were observed in the presence of oxygen, mechanisms pertaining to the participation of activated oxygen species may be ruled out. The oxidation of the G base is most likely associated with an oxygen atom transfer from the oxo-metallates to the double bond between C8 and N7 of the purine ring. The formation of the propenal may be associated with an oxygen-activated species, since a marginal enhancement of this product was observed in the presence of oxygen, The formation of furfural in higher abundance over 5-MF for dsDNA was attributed to the ease of hydrogen abstraction (or hydride transfer) from the C5' compared to C1' position of the ribose within a Cr(V)-DNA intermediate in which the metal center is bound to the phosphate diester moiety.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1093/nar/27.10.2219" target="_blank" rel="noreferrer noopener">10.1093/nar/27.10.2219</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1999
abstraction
aqueous-solution
Biochemistry & Molecular Biology
Bose R N
carcinogen chromium(vi)
Dudones L P
hydrogen
in-vitro
Joudah L
Journal Article or Conference Abstract Publication
Mazzer P A
Moghaddas S
molecular-oxygen
Nucleic acids research
nucleic-acids
radical formation
redox potentials
singlet oxygen
strand breaks
Stroup D
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A1471-A1471
Issue
7
Volume
13
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Dublin Core
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Title
A name given to the resource
The orphan nuclear hormone receptors, HNF4 and COUP-TFII, interact on the CYP7A1 promoter
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
1999-04
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Stroup D; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article
1999
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Faseb Journal
Journal Article
Life Sciences & Biomedicine - Other
Stroup D
Topics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
9833-9839
Issue
15
Volume
272
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Orphan receptors chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) and retinoid X receptor (RXR) activate and bind the rat cholesterol 7 alpha-hydroxylase gene (CYP7A)
Publisher
An entity responsible for making the resource available
Journal of Biological Chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
1997-04
Subject
The topic of the resource
arp-1; bile acids; Biochemistry & Molecular Biology; cloning; expression; liver; messenger-rna; regulatory protein-1; response elements; superfamily; thyroid-hormone
Creator
An entity primarily responsible for making the resource
Stroup D; Crestani M; Chiang J Y L
Description
An account of the resource
The cholesterol 7 alpha-hydroxylase gene (CYP7A) is transcriptionally regulated by a number of factors, including hormones, bile acids, and diurnal rhythm. Previous studies have identified a region from nucleotides (nt) -74 to -55 of the rat CYP7A promoter that enhanced bile acid repression of the SV40 early promoter, as assayed with a luciferase reporter gene in transiently transfected HepG2 cells. The rat CYP7A promoter/reporter activity was strongly stimulated by cotransfection with an expression plasmid encoding the nuclear hormone receptor chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) in a dose-dependent manner. Site-directed mutagenesis in the region of nt -74 to -55 altered this stimulation. Recombinant COUP-TFII expressed in HepG2 or COS-1 cells were found to bind to nt -74 -55 and nt -149 -128 probes by electrophoretic mobility shift assay (EMSA) and by supershifting the corresponding band with COUP-TFII-specific antibodies. The region of nt -176 -117 was previously mapped as a retinoic acid response region and was found to bind retinoid X receptor (RXR). EMSA supershift assays of wild-type and mutant oligomers using antibody against RXR revealed that the sequences between nt -145 and -134 were important for RXR binding. We conclude that COUP-TFII stimulates the transcriptional activity of the rat CYP7A promoter by binding to the sequences between nt -74 to -54 and nt -149 to -128. RXR may stimulate CYP7A gene transcription by binding to a direct repeat of the hormone response element separated by one nucleotide located at nt -146 -134.
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article
1997
arp-1
BILE acids
Biochemistry & Molecular Biology
Chiang J Y L
Cloning
Crestani M
expression
Journal Article
Journal of Biological Chemistry
Liver
messenger-rna
regulatory protein-1
Response Elements
Stroup D
superfamily
thyroid-hormone
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A1334-A1334
Issue
6
Volume
9
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
IDENTIFICATION OF A NEGATIVE REGULATORY ELEMENT IN THE CHOLESTEROL 7-ALPHA-HYDROXYLASE GENE (CYP7) PROXIMAL PROMOTER
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-04
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Stroup D; Crestani M; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article
1995
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Crestani M
Faseb Journal
Journal Article
Life Sciences & Biomedicine - Other
Stroup D
Topics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1-11
Issue
1
Volume
41
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
HNF4 and COUP-TFII interact to modulate transcription of the cholesterol 7 alpha-hydroxylase gene (CYP7A1)
Publisher
An entity responsible for making the resource available
Journal of Lipid Research
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-01
Subject
The topic of the resource
bile-acid synthesis; bile acids; Biochemistry & Molecular Biology; chicken ovalbumin; cholesterol metabolism; dna-binding; hepatocyte nuclear factor 4; hepatocyte nuclear factor 4; hormone-receptor superfamily; messenger-rna; orphan receptors; promoter; receptors; response elements; retinoic acid; thyroid-hormone; transcriptional regulation; upstream promoter transcription factor II
Creator
An entity primarily responsible for making the resource
Stroup D; Chiang J Y L
Description
An account of the resource
The gene for cholesterol 7 alpha-hydroxylase (CYP7A1) contains a sequence at nt -149 to -118 that was found to play a large role in determining the overall transcriptional activity and regulation of the promoter. Hepatocyte nuclear factor 4 (HNF4) and chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) synergistically activate transcription of the CYP7A1 promoter, Transactivation of CYP7A1 by HNF4 in the human hepatoma cell line, HepG2, was enhanced by cotransfection with COUP-TFII or the basal transcription element binding protein (BTEB), HNF4 prepared from rat liver nuclear extracts bound to oligomers homologous to the nt -146 to -134 sequences in electrophoretic mobility shift assays (EMSA), which corresponded to a conserved region containing a direct repeat of hormone response elements spaced by one nucleotide (DR1), The sequences surrounding this DR1 were found to be essential for the HNF4 transactivation. In vitro-translated COUP-TFII was found to bind the adjacent sequences from nt -139 to -128 (DRO), but COUP-TFII interacted with this region at a much lower affinity than to the COUP-TFII-site at nt -72 to -57 (DR4), Mutations at nt -139 to -128 or nt -72 to -57 reduced the COUP-TFII and HNF4 synergy; however, these COUP-TFII-binding sequences were not absolutely required for the cooperative effect of HNF4 and COUP-TFII on transactivation. These results indicated that the observed transactivation was the result of protein/protein interactions facilitated by the juxtaposition of the binding elements.
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2000
BILE acids
bile-acid synthesis
Biochemistry & Molecular Biology
Chiang J Y L
chicken ovalbumin
cholesterol metabolism
dna-binding
Hepatocyte Nuclear Factor 4
hormone-receptor superfamily
Journal Article
Journal of lipid research
messenger-rna
orphan receptors
promoter
Receptors
Response Elements
retinoic acid
Stroup D
thyroid-hormone
transcriptional regulation
upstream promoter transcription factor II
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A1390-A1390
Issue
8
Volume
12
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Hepatocyte nuclear factor 4 binds the bile acid response element II of the rat cholesterol 7 alpha-hydroxylase gene
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1998
1998-04
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Stroup D; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article
1998
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Faseb Journal
Journal Article
Life Sciences & Biomedicine - Other
Stroup D
Topics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
76-76
Volume
8
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
THE BILE-ACID REPRESSION OF CHOLESTEROL 7-ALPHA-HYDROXYLASE GENE-TRANSCRIPTION MAY BE MEDIATED THROUGH MULTIPLE ELEMENTS IN THE PROMOTER
Publisher
An entity responsible for making the resource available
Protein Engineering
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995
Subject
The topic of the resource
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
Creator
An entity primarily responsible for making the resource
Stroup D; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article
1995
Biochemistry & Molecular Biology
Biotechnology & Applied Microbiology
Chiang J Y L
Journal Article
Protein Engineering
Stroup D
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A1250-A1250
Issue
7
Volume
8
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
THE BILE-ACID RESPONSIVE ELEMENT IS LOCATED IN THE PROXIMAL PROMOTER OF THE CHOLESTEROL 7-ALPHA-HYDROXYLASE GENE (CYP7)
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-04
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Stroup D; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article
1994
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Faseb Journal
Journal Article
Life Sciences & Biomedicine - Other
Stroup D
Topics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
1831–1841
Issue
9
Volume
37
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene (CYP7A) in HepG2 cells.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-09
Subject
The topic of the resource
Humans; Binding Sites; Gene Expression Regulation; Cell Line; Transfection; Base Sequence; Molecular Sequence Data; Phorbol Esters/pharmacology; DNA-Binding Proteins/genetics/metabolism; *Transcription Factors; Enzyme Repression; Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics; Consensus Sequence; Glucocorticoids/pharmacology; Hepatocyte Nuclear Factor 3-alpha; Insulin/pharmacology; Nuclear Proteins/genetics/metabolism; Recombinant Fusion Proteins/biosynthesis; Thyroid Hormones/pharmacology; Genes; Receptors; Enzymologic/*drug effects; Genetic; *Promoter Regions; Reporter; *Transcription; Glucocorticoid/genetics/metabolism
Creator
An entity primarily responsible for making the resource
Wang D P; Stroup D; Marrapodi M; Crestani M; Galli G; Chiang J Y
Description
An account of the resource
A stable HepG2 cell line harboring a human cholesterol 7 alpha-hydroxylase (CYP7A) minigene/luciferase reporter gene construct was selected for studying transcriptional regulation of CYP7A gene promoter. Insulin and phorbol
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
*Transcription
*Transcription Factors
1996
Base Sequence
Binding Sites
Cell Line
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics
Consensus Sequence
Crestani M
Department of Integrative Medical Sciences
DNA-Binding Proteins/genetics/metabolism
Enzyme Repression
Enzymologic/*drug effects
Galli G
Gene Expression Regulation
Genes
Genetic
Glucocorticoid/genetics/metabolism
Glucocorticoids/pharmacology
Hepatocyte Nuclear Factor 3-alpha
Humans
Insulin/pharmacology
Journal of lipid research
Marrapodi M
Molecular Sequence Data
NEOMED College of Medicine
Nuclear Proteins/genetics/metabolism
Phorbol Esters/pharmacology
Receptors
Recombinant Fusion Proteins/biosynthesis
Reporter
Stroup D
Thyroid Hormones/pharmacology
Transfection
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
9833–9839
Issue
15
Volume
272
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Orphan receptors chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) and retinoid X receptor (RXR) activate and bind the rat cholesterol 7alpha-hydroxylase gene (CYP7A).
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
1997-04
Subject
The topic of the resource
Animals; Rats; *Gene Expression Regulation; Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism; Nuclear Proteins/*metabolism; Chickens; Transcription Factors/*metabolism; DNA-Binding Proteins/*metabolism; Retinoid X Receptors; COUP Transcription Factor II; COUP Transcription Factors; Circadian Rhythm; COUP Transcription Factor I; Recombinant Proteins/genetics/metabolism; Receptors; Models; Genetic; Enzymologic; Molecular; Electrophoresis; Polyacrylamide Gel; Promoter Regions; *Receptors; Steroid; Glucocorticoid/*genetics; Retinoic Acid/*metabolism
Creator
An entity primarily responsible for making the resource
Stroup D; Crestani M; Chiang J Y
Description
An account of the resource
The cholesterol 7alpha-hydroxylase gene (CYP7A) is transcriptionally regulated by a number of factors, including hormones, bile acids, and diurnal rhythm. Previous studies have identified a region from nucleotides (nt) -74 to -55 of the rat CYP7A promoter that enhanced bile acid repression of the SV40 early promoter, as assayed with a luciferase reporter gene in transiently transfected HepG2 cells. The rat CYP7A promoter/reporter activity was strongly stimulated by cotransfection with an expression plasmid encoding the nuclear hormone receptor chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) in a dose-dependent manner. Site-directed mutagenesis in the region of nt -74 to -55 altered this stimulation. Recombinant COUP-TFII expressed in HepG2 or COS-1 cells were found to bind to nt -74 -55 and nt -149 -128 probes by electrophoretic mobility shift assay (EMSA) and by supershifting the corresponding band with
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
*Receptors
1997
Animals
Chiang J Y
Chickens
Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism
Circadian Rhythm
COUP Transcription Factor I
COUP Transcription Factor II
COUP Transcription Factors
Crestani M
Department of Integrative Medical Sciences
DNA-Binding Proteins/*metabolism
Electrophoresis
Enzymologic
Genetic
Glucocorticoid/*genetics
Models
Molecular
NEOMED College of Medicine
Nuclear Proteins/*metabolism
Polyacrylamide Gel
Promoter Regions
Rats
Receptors
Recombinant Proteins/genetics/metabolism
Retinoic Acid/*metabolism
Retinoid X Receptors
Steroid
Stroup D
The Journal of biological chemistry
Transcription Factors/*metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
1–11
Issue
1
Volume
41
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
HNF4 and COUP-TFII interact to modulate transcription of the cholesterol 7alpha-hydroxylase gene (CYP7A1).
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-01
Subject
The topic of the resource
Humans; Animals; Rats; Liver/metabolism; Recombinant Proteins/metabolism; Base Sequence; Mutation; DNA Primers; Cholesterol 7-alpha-Hydroxylase/*genetics; Transcription Factors/*metabolism; Hepatocyte Nuclear Factor 4; DNA-Binding Proteins/*metabolism; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Luciferases/genetics; COUP Transcription Factor II; COUP Transcription Factors; Phosphoproteins/*metabolism; Genes; Sprague-Dawley; Cultured; Genetic; Tumor Cells; Reporter; Promoter Regions; *Transcription; *Receptors; Steroid
Creator
An entity primarily responsible for making the resource
Stroup D; Chiang J Y
Description
An account of the resource
The gene for cholesterol 7alpha-hydroxylase (CYP7A1) contains a sequence at nt -149 to -118 that was found to play a large role in determining the overall transcriptional activity and regulation of the promoter. Hepatocyte nuclear factor 4 (HNF4) and chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) synergistically activate transcription of the CYP7A1 promoter. Transactivation of CYP7A1 by HNF4 in the human hepatoma cell line, HepG2, was enhanced by cotransfection with COUP-TFII or the basal transcription element binding protein (BTEB). HNF4 prepared from rat liver nuclear extracts bound to oligomers homologous to the nt -146 to -134 sequences in electrophoretic mobility shift assays (EMSA), which corresponded to a conserved region containing a direct repeat of hormone response elements spaced by one nucleotide (DR1). The sequences surrounding this DR1 were found to be essential for the HNF4 transactivation. In vitro-translated COUP-TFII was found to bind the adjacent sequences from nt -139 to -128 (DR0), but COUP-TFII interacted with this region at a much lower affinity than to the COUP-TFII-site at nt -72 to -57 (DR4). Mutations at nt -139 to -128 or nt -72 to -57 reduced the COUP-TFII and HNF4 synergy; however, these
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Receptors
*Transcription
2000
Animals
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
COUP Transcription Factor II
COUP Transcription Factors
Cultured
Department of Integrative Medical Sciences
DNA Primers
DNA-Binding Proteins/*metabolism
Genes
Genetic
Hepatocyte Nuclear Factor 4
Humans
Journal of lipid research
Liver/metabolism
Luciferases/genetics
Mutation
NEOMED College of Medicine
Phosphoproteins/*metabolism
Promoter Regions
Rats
Recombinant Proteins/metabolism
Reporter
Sprague-Dawley
Steroid
Stroup D
Transcription Factors/*metabolism
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
2192–2200
Issue
11
Volume
39
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional activation of the cholesterol 7alpha-hydroxylase gene (CYP7A) by nuclear hormone receptors.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1998
1998-11
Subject
The topic of the resource
Animals; Rats; Transcription Factors/metabolism; Base Sequence; Molecular Sequence Data; DNA/metabolism; Cholesterol 7-alpha-Hydroxylase/*genetics; Hepatocyte Nuclear Factor 4; Mutagenesis; Luciferases/genetics; Retinoid X Receptors; Phosphoproteins/metabolism; COUP Transcription Factor II; COUP Transcription Factors; *Transcriptional Activation; Bile Acids and Salts/biosynthesis; DNA-Binding Proteins/metabolism; Hormones/*physiology; Oligonucleotide Probes/metabolism; Genes; Cultured; Receptors; Genetic; Cytoplasmic and Nuclear/*physiology; Tumor Cells; Reporter; Retinoic Acid/metabolism; Promoter Regions; Nucleic Acid; Site-Directed; *Receptors; Repetitive Sequences; Steroid
Creator
An entity primarily responsible for making the resource
Crestani M; Sadeghpour A; Stroup D; Galli G; Chiang J Y
Description
An account of the resource
The gene encoding cholesterol 7alpha-hydroxylase (CYP7A), the rate-limiting enzyme in bile acid synthesis, is transcriptionally regulated by bile acids and hormones. Previously, we have identified two bile acid response elements (BARE) in the promoter of the CYP7A gene. The BARE II is located in nt -149/-118 region and contains three hormone response element (HRE)-like sequences that form two overlapping nuclear receptor binding sites. One is a direct repeat separated by one nucleotide DR1 (-146- TGGACTtAGTTCA-134) and the other is a direct repeat separated by five nucleotides DR5 (-139-AGTTCAaggccGGG TAA-123). Mutagenesis of these HRE sequences resulted in lower transcriptional activity of the CYP7A promoter/reporter genes in transient transfection assay in HepG2 cells. The orphan nuclear receptor, hepatocyte nuclear factor 4 (HNF-4)1, binds to the DR1 sequence as assessed by electrophoretic mobility shift assay, and activates the CYP7A promoter/reporter activity by about 9-fold. Cotransfection of HNF-4 plasmid with another orphan nuclear receptor, chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII), synergistically activated the CYP7A transcription by 80-fold. The DR5 binds the RXR/RAR heterodimer. A hepatocyte nuclear factor-3 (HNF-3) binding site (-175-TGTTTGTTCT-166) was identified. HNF-3 was required for both basal transcriptional activity and stimulation of the rat CYP7A promoter activity by retinoic acid. Combinatorial interactions and binding of these transcription factors to BAREs may modulate the promoter activity and also mediate bile acid repression of CYP7A gene transcription.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Receptors
*Transcriptional Activation
1998
Animals
Base Sequence
Bile Acids and Salts/biosynthesis
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
COUP Transcription Factor II
COUP Transcription Factors
Crestani M
Cultured
Cytoplasmic and Nuclear/*physiology
Department of Integrative Medical Sciences
DNA-Binding Proteins/metabolism
DNA/metabolism
Galli G
Genes
Genetic
Hepatocyte Nuclear Factor 4
Hormones/*physiology
Journal of lipid research
Luciferases/genetics
Molecular Sequence Data
Mutagenesis
NEOMED College of Medicine
Nucleic Acid
Oligonucleotide Probes/metabolism
Phosphoproteins/metabolism
Promoter Regions
Rats
Receptors
Repetitive Sequences
Reporter
Retinoic Acid/metabolism
Retinoid X Receptors
Sadeghpour A
Site-Directed
Steroid
Stroup D
Transcription Factors/metabolism
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
2419–2432
Issue
11
Volume
36
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Hormonal regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7).
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-11
Subject
The topic of the resource
Animals; Rats; Gene Expression Regulation; Cell Count; Transfection; Base Sequence; Molecular Sequence Data; Phorbol Esters/pharmacology; Cholesterol 7-alpha-Hydroxylase/*genetics; Luciferases/genetics; Cyclic AMP/pharmacology; Hormones/*pharmacology; Second Messenger Systems/physiology; Cultured; Genetic; Tumor Cells; Cloning; Molecular; *Promoter Regions; *Transcription; Enzymologic/*physiology
Creator
An entity primarily responsible for making the resource
Crestani M; Stroup D; Chiang J Y
Description
An account of the resource
The transcriptional regulation of the rat cholesterol 7 alpha-hydroxylase gene (CYP7) by hormones and signal transduction pathways was studied by transient transfection assay of the promoter activity. HepG2 cells were transfected with deletion mutants of the CYP7 upstream region linked to the luciferase reporter gene. The transcription of CYP7/luciferase chimeric genes was higher in confluent than in subconfluent cultures of HepG2 cells. Glucocorticoid receptors, in the presence of dexamethasone, up-regulated the CYP7 gene through two regions located between -3262 and -2803, and between -344 and -222, respectively. Thyroid hormones did not have any effect on the promoter activity. Insulin inhibited the promoter activity through sequences located between -344 and -222, and abolished the stimulation by dexamethasone. Hence, the insulin effect was dominant over that of glucocorticoids. Treatment of transfected HepG2 cells with phorbol
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
*Transcription
1995
Animals
Base Sequence
Cell Count
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cloning
Crestani M
Cultured
Cyclic AMP/pharmacology
Department of Integrative Medical Sciences
Enzymologic/*physiology
Gene Expression Regulation
Genetic
Hormones/*pharmacology
Journal of lipid research
Luciferases/genetics
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Phorbol Esters/pharmacology
Rats
Second Messenger Systems/physiology
Stroup D
Transfection
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
257–265
Issue
1
Volume
262
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription by the liver orphan receptor (LXRalpha).
Publisher
An entity responsible for making the resource available
Gene
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-01
Subject
The topic of the resource
Humans; Animals; Binding Sites; Rats; Species Specificity; Transfection; Gene Expression Regulation/drug effects; Organ Specificity; Transcription Factors/genetics/metabolism; Cricetinae; Response Elements; Luciferases/genetics/metabolism; Retinoid X Receptors; Cholesterol 7-alpha-Hydroxylase/drug effects/*genetics/metabolism; Hydroxycholesterols; Liver/physiology; Lovastatin/pharmacology; Mevalonic Acid/metabolism/pharmacology; Nicotinic Acids/pharmacology; Polyisoprenyl Phosphates/pharmacology; Tetrahydronaphthalenes/pharmacology; Cells; Cultured; Receptors; Transcription; Genetic; Retinoic Acid/genetics/metabolism; Steroid/genetics/*metabolism
Creator
An entity primarily responsible for making the resource
Chiang J Y; Kimmel R; Stroup D
Description
An account of the resource
The cholesterol 7alpha-hydroxylase gene (CYP7A1) plays an important role in regulation of bile acid biosynthesis and cholesterol homeostasis. Oxysterol receptor, LXR, stimulates, whereas the bile acid receptor, FXR, inhibits CYP7A1 transcription. The goal of this study was to investigate the role of LXRalpha on the regulation of rat, human and hamster CYP7A1 transcription in its native promoter and cellular context. Cotransfection with LXRalpha and RXRalpha expression plasmids strongly stimulated rat CYP7A1/luciferase reporter activity in HepG2 cells and oxysterol was not required. However, LXRalpha had much less effect on hamster and no significant effect on human CYP7A1 promoter activity in HepG2 cells. In Chinese hamster ovary cells, cotransfection with LXRalpha stimulated reporter activity by less than 2-fold and addition of 22(R)-hydroxycholesterol caused a small but significant stimulation of rat, human and hamster CYP7A1 promoter activity. At least two direct repeats of AGGTCA-like sequences with 4-base spacing (DR4) and five-base spacing (DR5), in previously identified bile acid response elements of the rat CYP7A1 were able to bind LXRalpha/RXRalpha and confer LXRalpha stimulation. However, LXRalpha did not bind to the corresponding sequences of the human gene and bound weakly to hamster and mouse DR4 sequences. Therefore, rats and mice have the unusual capacity to convert cholesterol to bile acids by LXRalpha-mediated stimulation of CYP7A1 transcription, whereas other species do not respond to cholesterol and develop hypercholesterolemia on a diet high in cholesterol.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2001
Animals
Binding Sites
Cells
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/drug effects/*genetics/metabolism
Cricetinae
Cultured
Department of Integrative Medical Sciences
gene
Gene Expression Regulation/drug effects
Genetic
Humans
Hydroxycholesterols
Kimmel R
Liver/physiology
Lovastatin/pharmacology
Luciferases/genetics/metabolism
Mevalonic Acid/metabolism/pharmacology
NEOMED College of Medicine
Nicotinic Acids/pharmacology
Organ Specificity
Polyisoprenyl Phosphates/pharmacology
Rats
Receptors
Response Elements
Retinoic Acid/genetics/metabolism
Retinoid X Receptors
Species Specificity
Steroid/genetics/*metabolism
Stroup D
Tetrahydronaphthalenes/pharmacology
Transcription
Transcription Factors/genetics/metabolism
Transfection
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
17502–17507
Issue
26
Volume
269
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identification and characterization of a putative bile acid-responsive element in cholesterol 7 alpha-hydroxylase gene promoter.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-07
Subject
The topic of the resource
Humans; Animals; Rats; Gene Expression Regulation; Base Sequence; Bile Acids and Salts/*metabolism; Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism; Molecular Sequence Data; Recombinant Fusion Proteins/genetics/metabolism; Luciferases/genetics; DNA-Binding Proteins/metabolism; Deoxyribonuclease I; Nuclear Proteins/metabolism; Oligodeoxyribonucleotides; Thyroid Hormones/genetics; Sprague-Dawley; Genetic; Enzymologic; Cloning; Molecular; *Promoter Regions; Antigens; Polyomavirus Transforming/genetics
Creator
An entity primarily responsible for making the resource
Chiang J Y; Stroup D
Description
An account of the resource
Nucleotide sequences of a 7997-base pair SacI fragment spanning 3643 base pairs of the upstream promoter region to exon 4 of the rat cholesterol 7 alpha-hydroxylase gene (CYP7) have been determined. DNase I footprinting and electrophoretic mobility shift assay of the proximal promoter from nucleotides -346 to +36 revealed two protected regions which specifically shifted proteins in rat liver nuclear extracts. Footprint A (nucleotides -81 to -35) contained a cluster of overlapping sequence motifs of TGT3, steroid/thyroid hormone response elements (7 alpha TRE), hepatocyte nuclear factors 1 and 4, and CAAT/enhancer-binding protein alpha and has been shown to confer bile acid repression of the CYP7 gene promoter activity. Footprint B (nucleotides -148 to -129) contained a sequence motif HNF4. When footprint A (-101 to -49) or 7 alpha TRE (-73 to -55) sequence was linked upstream to a heterologous SV40 promoter/luciferase plasmid and transiently transfected into HepG2 cells, taurodeoxycholate suppressed the SV40 promoter activity. Electrophoretic mobility shift assays revealed that one or two bands shifted by the 7 alpha TRE or by a direct repeat sequence in 7 alpha TRE were absent when liver nuclear extracts of deoxycholic acid-treated rats were used. Similar gel shift patterns were also observed when human 7 alpha TRE or human liver nuclear extracts were used. The rat direct repeat sequence interacted with two polypeptides (M(r) = 57,000 and 116,000) in both rat and human liver nuclear extracts. These results suggest that hydrophobic bile acids may suppress the CYP7 gene expression by binding to a bile acid receptor which interacts with and prevents the binding of liver nuclear protein(s) to a bile acid-responsive element and that the core of bile acid-responsive element is a direct repeat.
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
1994
Animals
Antigens
Base Sequence
Bile Acids and Salts/*metabolism
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism
Cloning
Deoxyribonuclease I
Department of Integrative Medical Sciences
DNA-Binding Proteins/metabolism
Enzymologic
Gene Expression Regulation
Genetic
Humans
Luciferases/genetics
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Nuclear Proteins/metabolism
Oligodeoxyribonucleotides
Polyomavirus Transforming/genetics
Rats
Recombinant Fusion Proteins/genetics/metabolism
Sprague-Dawley
Stroup D
The Journal of biological chemistry
Thyroid Hormones/genetics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
10918–10924
Issue
15
Volume
275
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Farnesoid X receptor responds to bile acids and represses cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-04
Subject
The topic of the resource
Humans; Animals; Cricetinae; Cholesterol 7-alpha-Hydroxylase/*genetics; Response Elements; Bile Acids and Salts/*pharmacology; Transcription Factors/genetics/*physiology; Retinoid X Receptors; DNA-Binding Proteins/*physiology; Repressor Proteins/*physiology; Cultured; Receptors; Genetic; Tumor Cells; Promoter Regions; *Transcription; Cytoplasmic and Nuclear; Retinoic Acid/genetics
Creator
An entity primarily responsible for making the resource
Chiang J Y; Kimmel R; Weinberger C; Stroup D
Description
An account of the resource
Cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription is repressed by bile acids. The goal of this study is to elucidate the mechanism of CYP7A1 transcription by bile acid-activated farnesoid X receptor (FXR) in its native promoter and cellular context and to identify FXR response elements in the gene. In Chinese hamster ovary cells transfected with retinoid X receptor alpha (RXRalpha)/FXR, only chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA) were able to stimulate a heterologous promoter/reporter containing an ecdysone response element. In HepG2 cells, all bile acids (25 microM) were able to repress CYP7A1/luciferase reporter activity, and only CDCA and DCA further repressed reporter activity when cotransfected with RXRalpha/FXR. The concentration of CDCA required to inhibit 50% of reporter activity (IC(50)) was determined to be approximately 25 microM without FXR and 10 microM with FXR. Deletion analysis revealed that the bile acid response element located between nucleotides -148 and -128 was the FXR response element, but RXRalpha/FXR did not bind to this sequence. These results suggest that bile acid-activated FXR exerts its inhibitory effect on CYP7A1 transcription by an indirect mechanism, in contrast to the stimulation and binding of FXR to intestinal bile acid-binding protein gene promoter. Results also reveal that bile acid receptors other than FXR are present in HepG2 cells.
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Transcription
2000
Animals
Bile Acids and Salts/*pharmacology
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cricetinae
Cultured
Cytoplasmic and Nuclear
Department of Integrative Medical Sciences
DNA-Binding Proteins/*physiology
Genetic
Humans
Kimmel R
NEOMED College of Medicine
Promoter Regions
Receptors
Repressor Proteins/*physiology
Response Elements
Retinoic Acid/genetics
Retinoid X Receptors
Stroup D
The Journal of biological chemistry
Transcription Factors/genetics/*physiology
Tumor Cells
Weinberger C
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
1402–1412
Issue
9
Volume
42
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Nuclear receptor-mediated repression of human cholesterol 7alpha-hydroxylase gene transcription by bile acids.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-09
Subject
The topic of the resource
Humans; Animals; Rats; Cell Line; Transfection; Liver/metabolism; Reverse Transcriptase Polymerase Chain Reaction; DNA/metabolism; CHO Cells; Cricetinae; Cholesterol 7-alpha-Hydroxylase/*genetics; Bile Acids and Salts/*pharmacology; *Membrane Glycoproteins; *Hydroxysteroid Dehydrogenases; Caco-2 Cells; Carrier Proteins/genetics/physiology; DNA-Binding Proteins/drug effects/genetics/physiology; Gene Expression/*drug effects; Kidney; Luciferases/genetics; Recombinant Fusion Proteins/metabolism; Retinoid X Receptors; Taurocholic Acid/pharmacology; Transcription Factors/drug effects/genetics/physiology; Cultured; Receptors; RNA; Genetic/drug effects; Messenger/analysis; Transcription; Genetic; Tumor Cells; Promoter Regions; Embryo; Cytoplasmic and Nuclear/genetics/*physiology; Mammalian; Retinoic Acid/genetics/physiology
Creator
An entity primarily responsible for making the resource
Chen W; Owsley E; Yang Y; Stroup D; Chiang J Y
Description
An account of the resource
Hydrophobic bile acids strongly repressed transcription of the human cholesterol 7alpha-hydroxylase gene (CYP7A1) in the bile acid biosynthetic pathway in the liver. Farnesoid X receptor (FXR) repressed CYP7A1/Luc reporter activity in a transfection assay in human liver-derived HepG2 cells, but not in human embryonic kidney (HEK) 293 cells. FXR-binding activity was required for bile acid repression of CYP7A1 transcription despite the fact that FXR did not bind to the CYP7A1 promoter. FXR-induced liver-specific factors must be required for mediating bile acid repression. Bile acids and FXR repressed endogenous CYP7A1 but stimulated alpha-fetoprotein transcription factor (FTF) and small heterodimer partner (SHP) mRNA expression in HepG2 cells. Feeding of rats with chenodeoxycholic acid repressed CYP7A1, induced FTF, but had no effect on SHP mRNA expression in the liver. FTF strongly repressed CYP7A1 transcription in a dose-dependent manner, and SHP further inhibited CYP7A1 in HepG2 cells, but not in HEK 293 cells. FXR only moderately stimulated SHP transcription, whereas FTF strongly inhibited SHP transcription in HepG2 cells. Results revealed that FTF was a dominant negative factor that was induced by bile acid-activated FXR to inhibit both CYP7A1 and SHP transcription. Differential regulation of FTF and SHP expression by bile acids may explain the wide variation in CYP7A1 expression and the rate of bile acid synthesis and regulation in different species.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Hydroxysteroid Dehydrogenases
*Membrane Glycoproteins
2001
Animals
Bile Acids and Salts/*pharmacology
Caco-2 Cells
Carrier Proteins/genetics/physiology
Cell Line
Chen W
Chiang J Y
CHO Cells
Cholesterol 7-alpha-Hydroxylase/*genetics
Cricetinae
Cultured
Cytoplasmic and Nuclear/genetics/*physiology
Department of Integrative Medical Sciences
DNA-Binding Proteins/drug effects/genetics/physiology
DNA/metabolism
Embryo
Gene Expression/*drug effects
Genetic
Genetic/drug effects
Humans
Journal of lipid research
Kidney
Liver/metabolism
Luciferases/genetics
Mammalian
Messenger/analysis
NEOMED College of Medicine
Owsley E
Promoter Regions
Rats
Receptors
Recombinant Fusion Proteins/metabolism
Retinoic Acid/genetics/physiology
Retinoid X Receptors
Reverse Transcriptase Polymerase Chain Reaction
RNA
Stroup D
Taurocholic Acid/pharmacology
Transcription
Transcription Factors/drug effects/genetics/physiology
Transfection
Tumor Cells
Yang Y
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajpgi.1997.273.2.G508" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajpgi.1997.273.2.G508</a>
Pages
G508–517
Issue
2
Volume
273
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identification of a bile acid response element in the cholesterol 7 alpha-hydroxylase gene CYP7A.
Publisher
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The American journal of physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
1997-08
Subject
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Animals; Base Sequence; Bile Acids and Salts/*pharmacology; Cholesterol 7-alpha-Hydroxylase/*genetics; Cultured; DNA-Binding Proteins/metabolism; Feedback; Genes; Genes/*drug effects; Luciferases/genetics; Rats; Reporter; Tumor Cells
Creator
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Stroup D; Crestani M; Chiang J Y
Description
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The transcriptional activity of the cholesterol 7 alpha-hydroxylase gene CYP7A is repressed by bile acids. Taurine conjugates of chenodeoxycholate and deoxycholate, but not cholate and ursodeoxycholate, inhibited the CYP7A promoter/luciferase reporter activity in transient transfection assays in Hep G2 cells. A region from nucleotide (nt) -74 to -55 was found to mediate bile acid response. However, deletion of this bile acid response element (BARE-I) enhanced reporter activity but did not eliminate the bile acid response. This is due to the presence of another BARE-II located in a conserved region between nt -149 and -128. Deletion or mutations of these sequences reduced promoter activity and abolished bile acid repression. This BARE-II shares an identical AGTTCAAG core sequence with BARE-I. Electrophoretic mobility shift assays of BARE-I and BARE-II probes using Hep G2 nuclear extract and the partially purified binding activity of nt -65/-54 DNA-affinity column revealed that the same or a similar nuclear protein might bind to both BAREs. BARE-II is the major BARE involved in the transcriptional repression of the CYP7A gene by hydrophobic bile acids.
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<a href="http://doi.org/10.1152/ajpgi.1997.273.2.G508" target="_blank" rel="noreferrer noopener">10.1152/ajpgi.1997.273.2.G508</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1997
Animals
Base Sequence
Bile Acids and Salts/*pharmacology
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Crestani M
Cultured
Department of Integrative Medical Sciences
DNA-Binding Proteins/metabolism
Feedback
Genes
Genes/*drug effects
Luciferases/genetics
NEOMED College of Medicine
Rats
Reporter
Stroup D
The American journal of physiology
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/bbrc.1998.9759" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/bbrc.1998.9759</a>
Pages
109–113
Issue
1
Volume
253
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Basic transcription element binding protein (BTEB) transactivates the cholesterol 7 alpha-hydroxylase gene (CYP7A).
Publisher
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Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1998
1998-12
Subject
The topic of the resource
*Gene Expression Regulation; Animals; Bile Acids and Salts/genetics; Binding Sites/genetics; Carcinoma; Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism; Complementary/analysis; Cultured; DNA; DNA-Binding Proteins/metabolism/*physiology; Electrophoresis; Enzyme Activation/genetics; Genetic; Hepatocellular; Humans; Kruppel-Like Transcription Factors; Liver/enzymology; Podophyllin/analogs & derivatives/metabolism; Podophyllotoxin/analogs & derivatives; Polyacrylamide Gel; Promoter Regions; Protein Binding/genetics; Rats; Transcription Factors/metabolism/*physiology; Tumor Cells
Creator
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Foti D; Stroup D; Chiang J Y
Description
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Cholesterol 7 alpha-hydroxylase catalyzes the first and rate-limiting step in the conversion of cholesterol to bile acids in the liver. Previously, we have identified two bile acid response elements located in nt -74 to -54 (BARE-I) and -148 to -118 (BARE-II) regions. The nucleotide sequences in these BAREs are highly conserved and shared a novel sequence, AGTTCAAG. To identify and isolate nuclear protein factors that bind to these BAREs, we have screened a human liver cDNA expression library with oligonucleotide probes containing the sequence from nt -149 to -127. Twenty positive clones were selected and purified. Partial nucleotide sequences of these clones were determined. Nucleotide homology search of DNA databases of the sequences of these clones revealed that sequence of one clone, G13, is identical to basic transcription element binding protein (BTEB), a GC box-binding protein of Sp1 family transcription factors known to regulate many cytochrome P450 genes. Electrophoretic mobility shift assays have identified a basic transcription element (BTE) in BARE-II and a Sp1 binding site located in the nt -100/-82 region of the CYP7A promoter. Transient transfection assays have confirmed that BTEB was able to transactivate the CYP7A promoter/luciferase chimergic gene.
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<a href="http://doi.org/10.1006/bbrc.1998.9759" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1998.9759</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
1998
Animals
Bile Acids and Salts/genetics
Binding Sites/genetics
Biochemical and biophysical research communications
Carcinoma
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism
Complementary/analysis
Cultured
Department of Integrative Medical Sciences
DNA
DNA-Binding Proteins/metabolism/*physiology
Electrophoresis
Enzyme Activation/genetics
Foti D
Genetic
Hepatocellular
Humans
Kruppel-Like Transcription Factors
Liver/enzymology
NEOMED College of Medicine
Podophyllin/analogs & derivatives/metabolism
Podophyllotoxin/analogs & derivatives
Polyacrylamide Gel
Promoter Regions
Protein Binding/genetics
Rats
Stroup D
Transcription Factors/metabolism/*physiology
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/bbrc.1996.1215" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/bbrc.1996.1215</a>
Pages
585–592
Issue
2
Volume
225
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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The opposing effects of retinoic acid and phorbol esters converge to a common response element in the promoter of the rat cholesterol 7 alpha-hydroxylase gene (CYP7A).
Publisher
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Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-08
Subject
The topic of the resource
*Promoter Regions; Animals; Base Sequence; Cell Line; Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism; DNA Primers; Genetic; Molecular Sequence Data; Mutagenesis; Rats; Signal Transduction; Tetradecanoylphorbol Acetate/*pharmacology; Tretinoin/*pharmacology
Creator
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Crestani M; Sadeghpour A; Stroup D; Galli G; Chiang J Y
Description
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The activity of the rat CYP7A/luciferase reporter gene was increased five-fold by all-trans retinoic acid (atRA) or 9-cis retinoic acid (9cRA) in transient transfection assay in HepG2 cells. Cotransfection with retinoid X receptor (RXR) stimulated the promoter activity in the absence of ligand, however, addition of atRA inhibited the transcriptional activity. Cotransfection with retinoic acid receptor (RAR) did not have much effect on CYP7A promoter activity. The CYP7A promoter, when linked upstream to the SV40/ luciferase reporter gene, strongly repressed the phorbol 12-myristate 13-acetate (PMA)-stimulated SV40/ luciferase reporter gene activity. The regions conferring the effects of RA and PMA were mapped to nt-176/ -117 and nt-148/-129, respectively. Several direct repeats of hormone response element (AGTTCA) in this region are required for RA response.
Identifier
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<a href="http://doi.org/10.1006/bbrc.1996.1215" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1996.1215</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
1996
Animals
Base Sequence
Biochemical and biophysical research communications
Cell Line
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism
Crestani M
Department of Integrative Medical Sciences
DNA Primers
Galli G
Genetic
Molecular Sequence Data
Mutagenesis
NEOMED College of Medicine
Rats
Sadeghpour A
Signal Transduction
Stroup D
Tetradecanoylphorbol Acetate/*pharmacology
Tretinoin/*pharmacology