1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1002/jcb.29168" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jcb.29168</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A novel regulatory role of TRAPPC9 in L-plastin-mediated osteoclast actin ring formation Publisher An entity responsible for making the resource available Journal of Cellular Biochemistry Date A point or period of time associated with an event in the lifecycle of the resource 2019 2019-08 Subject The topic of the resource actin ring; cytoskeleton; L-plastin; osteoclast; protein trafficking; TRAPPC9; TRAPPII Creator An entity primarily responsible for making the resource Hussein Nazar J; Mbimba Thomas; Al-Adlaan Asaad A; Ansari Mohammad Y; Jaber Fatima A; McDermott Scott; Kasumov Takhar; Safadi Fayez F Description An account of the resource Trafficking protein particle complex 9 (TRAPPC9) is a major subunit of the TRAPPII complex. TRAPPC9 has been reported to bind nuclear factor κB kinase subunit β (IKKβ) and NF-kB-inducing kinase (NIK) where it plays a role in the canonical and noncanonical of nuclear factor-κB (NF-kB) signaling pathways, receptively. The role of TRAPPC9 in protein trafficking and cytoskeleton organization in osteoclast (OC) has not been studied yet. In this study, we examined the mRNA expression of TRAPPC9 during OC differentiation. Next, we examined the colocalization of TRAPPC9 with cathepsin-K, known to mediate OC resorption suggesting that TRAPPC9 mediates the trafficking pathway within OC. To identify TRAPPC9 protein partners important for OC-mediated cytoskeleton re-organization, we conducted immunoprecipitation of TRAPPC9 in mature OCs followed by mass spectrometry analysis. Our data showed that TRAPPC9 binds various protein partners. One protein with high recovery rate is L-plastin (LPL). LPL localizes at the podosomes and reported to play a crucial role in actin aggregation thereby actin ring formation and OC function. Although the role of LPL in OC-mediated bone resorption has not fully reported in detail. Here, first, we confirmed the binding of LPL to TRAPPC9 and, then, we investigated the potential regulatory role of TRAPPC9 in LPL-mediated OC cytoskeleton reorganization. We assessed the localization of TRAPPC9 and LPL in OC and found that TRAPPC9 is colocalized with LPL at the periphery of OC. Next, we determined the effect of TRAPPC9 overexpression on LPL recruitment to the actin ring using a viral system. Interestingly, our data showed that TRAPPC9 overexpression promotes the recruitment of LPL to the actin ring when compared with control cultures. In addition, we observed that TRAPPC9 overexpression reorganizes actin clusters/aggregates and regulates vinculin recruitment into the OC periphery to initiate podosome formation. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1002/jcb.29168" target="_blank" rel="noreferrer noopener">10.1002/jcb.29168</a> 2019 actin ring Al-Adlaan Asaad A Ansari Mohammad Y cytoskeleton Department of Anatomy & Neurobiology Hussein Nazar J Jaber Fatima A Journal of cellular biochemistry Kasumov Takhar L-plastin Mbimba Thomas McDermott Scott NEOMED College of Medicine osteoclast protein trafficking Safadi Fayez F September 2019 Update TRAPPC9 TRAPPII