1
40
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1099/0022-1317-75-7-1743" target="_blank" rel="noreferrer noopener">http://doi.org/10.1099/0022-1317-75-7-1743</a>
Pages
1743–1747
Volume
75 ( Pt 7)
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Analysis of the thymidine kinase of a herpes simplex virus type 1 isolate that exhibits resistance to (E)-5-(2-bromovinyl)-2'-deoxyuridine.
Publisher
An entity responsible for making the resource available
The Journal of general virology
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-07
Subject
The topic of the resource
Acyclovir/pharmacology; Amino Acid Sequence; Antiviral Agents/metabolism/*pharmacology; Base Sequence; Binding Sites; Bromodeoxyuridine/*analogs & derivatives/metabolism/pharmacology; Deoxyuridine/analogs & derivatives/pharmacology; DNA; Drug Resistance; Herpesvirus 1; Human/drug effects/*enzymology/genetics; Humans; Kinetics; Microbial; Molecular Sequence Data; Phosphonoacetic Acid/pharmacology; Phosphorylation; Point Mutation/genetics; Sequence Analysis; Thymidine Kinase/genetics/metabolism; Thymidine/metabolism; Vidarabine/pharmacology
Creator
An entity primarily responsible for making the resource
Wilber B A; Docherty J J
Description
An account of the resource
The mechanism responsible for the decreased sensitivity of a clinical herpes simplex virus type 1 (HSV-1) isolate, HSV-145, to (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) was examined. Measurements of 50% inhibitory doses of several drugs demonstrated that although HSV-145 was sensitive to phosphonoacetic acid, adenine arabinoside and acyclovir, its sensitivity to BVDU and 5-(2-chloroethyl)-2'-deoxyuridine was significantly less than that normally observed for HSV-1. Analysis of the thymidylate kinase (TMP-K) activity of HSV-145 thymidine kinase (TK) demonstrated a decreased level of TMP-K activity when compared to HSV-1 TK. The TMP-K activity of HSV-145 resembled that observed for HSV-2 and the TK-deficient strain HSV-1 TK-7. When the nucleotide sequence of the HSV-145 TK gene was compared to that of the HSV-1 strains C1(101) and SC16 a single nucleotide substitution (G changed to A at base position 502) was detected which would result in the substitution of threonine at amino acid position 168 for alanine. The substitution is the same as that for the laboratory-derived BVDU-resistant virus HSV-1 SC16B3. Collectively, these studies highlight the importance of amino acid conservation at position 168 of the HSV-1 TK in conferring efficient TMP-K activity and BVDU sensitivity.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1099/0022-1317-75-7-1743" target="_blank" rel="noreferrer noopener">10.1099/0022-1317-75-7-1743</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1994
Acyclovir/pharmacology
Amino Acid Sequence
Antiviral Agents/metabolism/*pharmacology
Base Sequence
Binding Sites
Bromodeoxyuridine/*analogs & derivatives/metabolism/pharmacology
Deoxyuridine/analogs & derivatives/pharmacology
DNA
Docherty J J
Drug Resistance
Herpesvirus 1
Human/drug effects/*enzymology/genetics
Humans
Kinetics
Microbial
Molecular Sequence Data
Phosphonoacetic Acid/pharmacology
Phosphorylation
Point Mutation/genetics
Sequence Analysis
The Journal of general virology
Thymidine Kinase/genetics/metabolism
Thymidine/metabolism
Vidarabine/pharmacology
Wilber B A
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1099/0022-1317-70-4-857" target="_blank" rel="noreferrer noopener">http://doi.org/10.1099/0022-1317-70-4-857</a>
Pages
857–867
Volume
70 ( Pt 4)
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Mild acidic pH inhibition of the major pathway of herpes simplex virus entry into HEp-2 cells.
Publisher
An entity responsible for making the resource available
The Journal of general virology
Date
A point or period of time associated with an event in the lifecycle of the resource
1989
1989-04
Subject
The topic of the resource
Animals; Cell Line; Electron; Electrophoresis; Endocytosis; Glycoproteins/analysis; Glycosylation; Humans; Hydrogen-Ion Concentration; Microscopy; Polyacrylamide Gel; Simplexvirus/*physiology/ultrastructure; Temperature; Time Factors; Vero Cells; Viral Proteins/analysis/metabolism
Creator
An entity primarily responsible for making the resource
Rosenthal K S; Killius J; Hodnichak C M; Venetta T M; Gyurgyik L; Janiga K
Description
An account of the resource
Penetration of the KOS strain of herpes simplex virus type 1 (HSV-1) and the MS and 333 strains of herpes simplex virus type 2 (HSV-2) into HEp-2 cells at pH 6.3 was at least 100-fold less efficient than at pH 7.4. Penetration of two low passage clinical isolates was completely blocked at pH 6.3. The syncytium-forming
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1099/0022-1317-70-4-857" target="_blank" rel="noreferrer noopener">10.1099/0022-1317-70-4-857</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1989
Animals
Cell Line
Electron
Electrophoresis
Endocytosis
Glycoproteins/analysis
Glycosylation
Gyurgyik L
Hodnichak C M
Humans
Hydrogen-Ion Concentration
Janiga K
Killius J
Microscopy
Polyacrylamide Gel
Rosenthal K S
Simplexvirus/*physiology/ultrastructure
Temperature
The Journal of general virology
Time Factors
Venetta T M
Vero Cells
Viral Proteins/analysis/metabolism