1
40
3
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Text
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<a href="http://doi.org/10.7150/thno.30204" target="_blank" rel="noreferrer noopener">http://doi.org/10.7150/thno.30204</a>
Pages
898-909
Issue
2
Volume
10
ISSN
1838-7640
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<a href="http://neomed.idm.oclc.org/login?url=http://doi.org/10.7150/thno.30204" target="_blank" rel="noreferrer noopener">NEOMED Full-text Holding (if available) - Proxy DOI: 10.7150/thno.30204</a>
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Update Year & Number
June 2020 Update I
NEOMED College
NEOMED College of Medicine
NEOMED Department
NEOMED Student Publications
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Polymerase chain reaction - surface-enhanced Raman spectroscopy (PCR-SERS) method for gene methylation level detection in plasma
Publisher
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Theranostics
Date
A point or period of time associated with an event in the lifecycle of the resource
2020
2020
Subject
The topic of the resource
3-methyladenine; 5-methylcytosine; aberrant promoter methylation; classification; dna methylation; hypermethylation; methylation level; multiplex detection; pcr; plasma; point mutations; profile; scattering; sers; surface-enhanced Raman spectroscopy
Creator
An entity primarily responsible for making the resource
Li Xiaozhou; Yang Tianyue; Li Caesar Siqi; Song Youtao; Wang Deli; Jin Lili; Lou Hong; Li Wei
Description
An account of the resource
Gene promoter hypermethylation is a vital step in tumorigenesis. This paper set out to explore the use of polymerase chain reaction - surface-enhanced Raman spectroscopy (PCR-SERS) for the detection of gene methylation levels, with a focus on cancer diagnosis. Methods: PCR with methylation independent primers were used on DNA samples to amplify target genes regardless of their methylation states. SERS was used on the obtained PCR products to generate spectra that contained peak changes belonging to CG and AT base pairs. Multiple linear regression (MLR) was then used to deconvolute the SERS spectra so that the CG/AT ratios of the sample could be obtained. These MLR results were used to calculate methylation levels of the target genes. For protocol verification, three sets of seven reference DNA solutions with known methylation levels (0%, 1%, 5%, 25%, 50%, 75%, and 100%) were analysed. Clinically, blood plasma samples were taken from 48 non-small-cell lung cancer (NSCLC) patients and 51 healthy controls. The methylation levels of the genes p16, MGMT, and RASSF1 were determined for each patient using this method. Results: Verification experiment on the mixtures with known methylation levels resulted in an error of less than 6% from the actual levels. When applied to our clinical samples, the frequency of methylation in at least one of the three target genes among the NSCLC patients was 87.5%, but this percentage decreased to 11.8% for the control group. The methylation levels of p16 were found to be significantly higher in NSCLC patients with more pack-years smoked (p=0.04), later cancer stages (p=0.03), and cancer types of squamous cell and large cell versus adenocarcinoma (p=0.03). Prediction accuracy of 88% was achieved from classification and regression trees (CART) based on methylation levels and states, respectively. Conclusion: This research showed that the PCR-SERS protocol could quantitatively measure the methylation levels of genes in plasma. The methylation levels of the genes p16, MGMT, and RASSF1 were higher in NSCLC patients than in controls.
Identifier
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<a href="http://doi.org/10.7150/thno.30204" target="_blank" rel="noreferrer noopener">10.7150/thno.30204</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
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journalArticle
2020
3-methyladenine
5-methylcytosine
aberrant promoter methylation
classification
DNA Methylation
hypermethylation
Jin Lili
Journal Article
journalArticle
June 2020 Update I
Li Caesar Siqi
Li Wei
Li Xiaozhou
Lou Hong
methylation level
multiplex detection
NEOMED College of Medicine Student
NEOMED Student Publications
PCR
Plasma
point mutations
profile
Scattering
SERS
Song Youtao
surface-enhanced Raman spectroscopy
Theranostics
Wang Deli
Yang Tianyue
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1208/s12249-014-0097-8" target="_blank" rel="noreferrer noopener">http://doi.org/10.1208/s12249-014-0097-8</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
741-752
Issue
3
Volume
15
Search for Full-text
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<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Light-Activatable Gold Nanoshells for Drug Delivery Applications
Publisher
An entity responsible for making the resource available
Aaps Pharmscitech
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-06
Subject
The topic of the resource
cancer nanotheranostics; drug delivery; gold nanoshells; growth-factor receptor; in-vitro; nanocarrier; nanoparticles; nf-kappa-b; Pharmacology & Pharmacy; photothermal-chemotherapy; release; surface-plasmon resonance; targeted delivery; theranostics; thermal therapy; triggered; triggered release
Creator
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Singhana B; Slattery P; Chen A; Wallace M; Melancon M P
Description
An account of the resource
Gold nanoshells (AuNSs) are currently being investigated as nanocarriers for drug delivery systems and have both diagnostic and therapeutic applications, including photothermal ablation, hyperthermia, drug delivery, and diagnostic imaging, particularly in oncology. AuNSs are valuable for their localized surface plasmon resonance, biocompatibility, low immunogenicity, and facile functionalization. AuNSs used for drug delivery can be spatially and temporally triggered to release controlled quantities of drugs inside the target cells when illuminated with a near-infrared (NIR) laser. Recently, many research groups have demonstrated that these AuNS complexes are able to deliver antitumor drugs (e.g., doxorubicin, paclitaxel, small interfering RNA, and single-stranded DNA) into cancer cells, which enhances the efficacy of treatment. AuNSs can also be functionalized with active targeting ligands such as antibodies, aptamers, and peptides to increase the particles' specific binding to the desired targets. This article reviews the current research on NIR light-activatable AuNSs used as nanocarriers for drug delivery systems and cancer theranostics.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1208/s12249-014-0097-8" target="_blank" rel="noreferrer noopener">10.1208/s12249-014-0097-8</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2014
AAPS PharmSciTech
cancer nanotheranostics
Chen A
Drug delivery
gold nanoshells
growth-factor receptor
in-vitro
Journal Article
Melancon M P
nanocarrier
Nanoparticles
nf-kappa-b
Pharmacology & Pharmacy
photothermal-chemotherapy
release
Singhana B
Slattery P
surface-plasmon resonance
targeted delivery
Theranostics
thermal therapy
triggered
triggered release
Wallace M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.7150/thno.22502" target="_blank" rel="noreferrer noopener">http://doi.org/10.7150/thno.22502</a>
Pages
1678–1689
Issue
6
Volume
8
Dublin Core
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Title
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Surface Enhanced Raman Spectroscopy (SERS) for the Multiplex Detection of Braf, Kras, and Pik3ca Mutations in Plasma of Colorectal Cancer Patients.
Publisher
An entity responsible for making the resource available
Theranostics
Date
A point or period of time associated with an event in the lifecycle of the resource
2018
1905-07
Subject
The topic of the resource
Adult; Female; Humans; Male; Middle Aged; Aged; *colorectal cancer; *gene; *mutation; *Mutation; *PCR; *surface enhanced Raman scattering; Class I Phosphatidylinositol 3-Kinases/blood/*genetics; Cluster Analysis; Colorectal Neoplasms/blood/*diagnosis/genetics/pathology; DNA Primers/chemistry; Exons; Multiplex Polymerase Chain Reaction/methods/standards; Neoplasm Staging; Proto-Oncogene Proteins B-raf/blood/*genetics; Proto-Oncogene Proteins p21(ras)/blood/*genetics; Biomarkers; Spectrum Analysis; Raman/methods/*standards; Tumor/blood/*genetics
Creator
An entity primarily responsible for making the resource
Li Xiaozhou; Yang Tianyue; Li Caesar Siqi; Song Youtao; Lou Hong; Guan Dagang; Jin Lili
Description
An account of the resource
In this paper, we discuss the use of a procedure based on polymerase chain reaction (PCR) and surface enhanced Raman spectroscopy (SERS) (PCR-SERS) to detect DNA mutations. Methods: This method was implemented by first amplifying
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.7150/thno.22502" target="_blank" rel="noreferrer noopener">10.7150/thno.22502</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*colorectal cancer
*gene
*Mutation
*PCR
*surface enhanced Raman scattering
2018
Adult
Aged
Biomarkers
Class I Phosphatidylinositol 3-Kinases/blood/*genetics
Cluster Analysis
Colorectal Neoplasms/blood/*diagnosis/genetics/pathology
DNA Primers/chemistry
Exons
Female
Guan Dagang
Humans
Jin Lili
Li Caesar Siqi
Li Xiaozhou
Lou Hong
Male
Middle Aged
Multiplex Polymerase Chain Reaction/methods/standards
Neoplasm Staging
Proto-Oncogene Proteins B-raf/blood/*genetics
Proto-Oncogene Proteins p21(ras)/blood/*genetics
Raman/methods/*standards
Song Youtao
Spectrum Analysis
Theranostics
Tumor/blood/*genetics
Yang Tianyue