1
40
1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1159/000324642" target="_blank" rel="noreferrer noopener">http://doi.org/10.1159/000324642</a>
Pages
320–325
Issue
2
Volume
194
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The nature and role of periosteum in bone and cartilage regeneration.
Publisher
An entity responsible for making the resource available
Cells, tissues, organs
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011
Subject
The topic of the resource
Animals; Bone Regeneration/genetics/*physiology; Cartilage/*physiology; Cattle; Collagen Type I/genetics/metabolism; Collagen Type II/genetics/metabolism; Core Binding Factor Alpha 1 Subunit/genetics/metabolism; Gene Expression Regulation; Integrin-Binding Sialoprotein/genetics/metabolism; Mice; Nude; Periosteum/anatomy & histology/diagnostic imaging/*physiology; Prosthesis Implantation; Radiography; Tissue Engineering; Tissue Scaffolds
Creator
An entity primarily responsible for making the resource
Matsushima Seika; Isogai Noritaka; Jacquet Robin; Lowder Elizabeth; Tokui Taku; Landis William J
Description
An account of the resource
This study was undertaken to determine whether periosteum from different bone sources in a donor results in the same formation of bone and cartilage. In this case, periosteum obtained from the cranium and mandible (examples of tissue supporting intramembranous ossification) and the radius and ilium (examples of tissues supporting endochondral ossification) of individual calves was used to produce tissue-engineered constructs that were implanted in nude mice and then retrieved after 10 and 20 weeks. Specimens were compared in terms of their osteogenic and chondrogenic potential by radiography, histology, and gene expression levels. By 10 weeks of implantation and more so by 20 weeks, constructs with cranial periosteum had developed to the greatest extent, followed in order by ilium, radius, and mandible periosteum. All constructs, particularly with cranial tissue although minimally with mandibular periosteum, had mineralized by 10 weeks on radiography and stained for proteoglycans with safranin-O red (cranial tissue most intensely and mandibular tissue least intensely). Gene expression of type I collagen, type II collagen, runx2, and bone sialoprotein (BSP) was detectable on QRT-PCR for all specimens at 10 and 20 weeks. By 20 weeks, the relative gene levels were: type I collagen, ilium \textgreater\textgreater radial \textgreater/= cranial \textgreater/= mandibular; type II collagen, radial \textgreater ilium \textgreater cranial \textgreater/= mandibular; runx2, cranial \textgreater\textgreater\textgreater radial \textgreater mandibular \textgreater/= ilium; and BSP, ilium \textgreater/= radial \textgreater cranial \textgreater mandibular. These data demonstrate that the osteogenic and chondrogenic capacity of the various constructs is not identical and depends on the periosteal source regardless of intramembranous or endochondral ossification. Based on these results, cranial and mandibular periosteal tissues appear to enhance bone formation most and least prominently, respectively. The appropriate periosteal choice for bone and cartilage tissue engineering and regeneration should be a function of its immediate application as well as other factors besides growth rate.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1159/000324642" target="_blank" rel="noreferrer noopener">10.1159/000324642</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2011
Animals
Bone Regeneration/genetics/*physiology
Cartilage/*physiology
Cattle
Cells, tissues, organs
Collagen Type I/genetics/metabolism
Collagen Type II/genetics/metabolism
Core Binding Factor Alpha 1 Subunit/genetics/metabolism
Gene Expression Regulation
Integrin-Binding Sialoprotein/genetics/metabolism
Isogai Noritaka
Jacquet Robin
Landis William J
Lowder Elizabeth
Matsushima Seika
Mice
Nude
Periosteum/anatomy & histology/diagnostic imaging/*physiology
Prosthesis Implantation
Radiography
Tissue Engineering
Tissue Scaffolds
Tokui Taku