Activation Of The Interferon-inducing And Anti-viral Activities Of Polyribonucleotides By Intercalating Drugs
Biophysics
Jamison J M; Bonilla P J; Koo P H; Tsai C C
Biophysical Journal
1985
1985
Journal Article or Conference Abstract Publication
n/a
Antiviral activity of magnesium and magnesium/poly r(A-U) combinations against two RNA viruses.
Antiviral Agents/*pharmacology; HIV-1/*drug effects; Humans; Magnesium/*pharmacology; Microbial Sensitivity Tests; Poly A-U/*pharmacology; Vesicular stomatitis Indiana virus/*drug effects
Magnesium (Mg2+) potentiated the anti-vesicular stomatitis virus (VSV) activity of poly r(A-U) or poly r(G-C) and the anti-HIV-1 activity of poly r(A-U). Mg2+ did not affect the anti-VSV activity of poly (rI).poly (rC), poly (dA-dT).poly (dA-dT) or poly (dG-dC).poly (dG-dC). Modulation of one or more nuclear (nucleolar) processes of the host cell may be responsible for the synergistic antiviral activity.
Jamison J; Gilloteaux J; Nassiri M R; Tsai C C; Summers J
Nucleosides & nucleotides
1999
1999-07
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1080/07328319908044668" target="_blank" rel="noreferrer noopener">10.1080/07328319908044668</a>
Effects Of Intercalating Drugs On The Antiviral And Interferon-inducing Activities Of Polyribonucleotides
Biophysics
Jamison J M; Bonilla P J; Tsai C C
Biophysical Journal
1986
1986-02
Journal Article or Conference Abstract Publication
n/a
Enhancement Of The Antiviral Activity Of Poly (a-u) By Adriamycin And Daunomycin
Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Virology
The role of adriamycin (ADR) and daunomycin (DMN) in modulating the antiviral activity of poly (adeylate-uridylate) (poly (A-U)) was examined using a human foreskin fibroblast - vesicular stomatitis virus (HSF-VSV) bioassay in which the concentration of poly (A-U) was fixed at 0.05 mm or 0.2 mm while the ADR or DMN concentration was varied to produce ADR (or DMN)/ribonucleotide ratios ranging from 1:16 to 2:1. Poly (A-U), ADR and DMN were not efficacious antiviral agents when tested individually at the concentrations employed in the ADR (or DMN)/poly (A-U) combinations. When the ADR or DMN was combined with the poly (A-U) to produce ADR (or DMN)/poly (A-U) ratios of 1/6, the 50% effective doses (ED50) of the poly (A-U), ADR and DMN decreased 18, 104, and 185-fold, respectively. However, when ADR or DMN was combined with polyriboinosinic-polyribocytidylic acid [poly (I) . poly (C)], the ED50 of the ADR, DMN and the poly (I) . poly (C) were not affected. Interferon neutralization studies indicated that ADR, DMN, poly (A-U) and the ADR (or DMN)/poly (A-U) combination induced the production of interferon-beta (IFN-beta). The amount of IFN produced by the ADR (or DMN)/poly (A-U) combinations was equal to the sum of the IFN produced by their constituents. These results indicate that the ADR and DMN potentiate the antiviral activity of the poly (A-U) without affecting the amount of IFN induced. The direct viral inactivation study demonstrated that ADR, DMN, poly (A-U) and the ADR (or DMN)/poly (A-U) combinations do not inactivate the VSV at concentrations near the ED50.
Jamison J M; Bonilla P J; Tsai C C
Antiviral Chemistry & Chemotherapy
1990
1990-10
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1177/095632029000100502" target="_blank" rel="noreferrer noopener">10.1177/095632029000100502</a>
Enhancement Of The Antiviral Activity Of Poly R(a-u) By Ametantrone And Mitoxantrone
Pharmacology & Pharmacy; Research & Experimental Medicine
Jamison J M; Krabill K; Flowers D G; Tsai C C
Life Sciences
1990
1990
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0024-3205(90)90134-d" target="_blank" rel="noreferrer noopener">10.1016/0024-3205(90)90134-d</a>
Enhancement Of The Antiviral And Interferon-inducing Activities Of Poly-r(a-u) By Carminic Acid
Pharmacology & Pharmacy; Research & Experimental Medicine
Jamison J M; Flowers D G; Jamison E; Kitareewan S; Krabill K; Rosenthal K S; Tsai C C
Life Sciences
1988
1988
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0024-3205(88)90058-6" target="_blank" rel="noreferrer noopener">10.1016/0024-3205(88)90058-6</a>
In vivo and in vitro antiherpetic effects of hydroxytolans
Pharmacology & Pharmacy; Virology
Tsai C C; Lesniewski M L; Liu B; Parakulam R R; Fu M M; Docherty J J
Antiviral Research
2003
2003-02
Journal Article
n/a
In Vivo Anti-herpes Simplex Virus Activity Of Resveratrol, A Cyclin Dependent Kinase Gene Inhibitor
Pharmacology & Pharmacy; Virology
Docherty J J; Fu M M; Stoner T; Smith J; Lesniewski M; Tsai C C; Booth T
Antiviral Research
2003
2003-02
Journal Article or Conference Abstract Publication
n/a
Interferon Induction Invitro By Polynucleotides
Biophysics
Jamison J M; Koo P H; Tsai C C
Biophysical Journal
1984
1984
Journal Article or Conference Abstract Publication
n/a
Interferon Induction Invitro By The Polyribonucleotide, Poly R(a-u)
Environmental Sciences & Ecology; Zoology
Jamison J M; Koo P H; Tsai C C
Ohio Journal of Science
1984
1984
Journal Article or Conference Abstract Publication
n/a
Invitro Antiviral Activity Induced By Poly-r(a-u) And Intercalating Dyes
Biophysics
Jamison J M; Bonilla P J; Flowers D G; Kitareewan S; Tsai C C
Biophysical Journal
1987
1987-02
Journal Article or Conference Abstract Publication
n/a
Invitro Antiviral Activity Of Poly (a-u) And Ellipticines
Biochemistry & Molecular Biology
Jamison J; Krabill K; Flowers D; Tsai C C
Biochimie
1990
1990-04
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0300-9084(90)90078-u" target="_blank" rel="noreferrer noopener">10.1016/0300-9084(90)90078-u</a>
INVITRO ANTIVIRAL ACTIVITY STUDIES OF POLY-R(A-U) AND INTERCALATING AGENTS
Pharmacology & Pharmacy; Virology
Tsai C C; Jamison J M; Flowers D G; Kitareewan S
Antiviral Research
1988
1988-01
Journal Article
n/a
Modulation Of The Antiviral Activity Of Poly (a-u) By Ethidium Bromide And Propidium Iodide
Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Virology
The role of ethidium bromide (EB) and propidium iodide (PI) in modulating the antiviral and interferon-inducing activities of poly(adenylate-uridylate) (poly (A-U )) was examined using the human foreskin fibro-blast-vesicular stomatitis virus (HSF-VSV) bioassay system in which the concentration of poly (A-U) was fixed at 0.05 mM or 0.2 mM while the EB or PI concentration was varied to produce variable EB (or PI)/ribonucleotide ratios ranging from 1: 16 to 2:1. EB, PI and poly (A-U) tested individually were not efficacious antiviral agents. When poly (A-U) was combined with the ethidium bromide or propidium iodide the antiviral activity was potentiated 15- to 22-fold at EB (or Pl)/ribonucleotide ratios in the region of 1/4. The interferon-inducing activity of the EB (or Pl)/poly (A-U) combinations were equal to the sum of the interferon-inducing activity of the poly (A-U) and the EB or (PI). These results indicate that the EB and PI potentiate the antiviral activity of the poly (A-U) without superinduction of interferon. The direct viral inactivation study demonstrated that EB, PI, poly (A-U) and the EB (or Pl)/poly (A-U) combinations did not inactivate the VSV at concentrations near the 50% viral inhibitory dose.
Jamison J M; Bonilla P J; Tsai C C
Antiviral Chemistry & Chemotherapy
1990
1990-02
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1177/095632029000100109" target="_blank" rel="noreferrer noopener">10.1177/095632029000100109</a>
Polyribonucleotide-anthraquinone Interactions - Invitro Antiviral Activity Studies
Cell Biology
Jamison J M; Krabill K; Flowers D G; Tsai C C
Cell Biology International Reports
1990
1990-03
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/s0309-1651(05)80004-9" target="_blank" rel="noreferrer noopener">10.1016/s0309-1651(05)80004-9</a>
Potentiation Of The Antiviral Activity Of Poly R(a-u) By Riboflavin, Fad And Fmn
Cell Biology
Jamison J M; Flowers D G; Kitareewan S; Krabill K; Tsai C C
Cell Biology International Reports
1989
1989-02
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0309-1651(89)90068-4" target="_blank" rel="noreferrer noopener">10.1016/0309-1651(89)90068-4</a>
Potentiation Of The Antiviral Activity Of Poly R(a-u) By Xanthene Dyes
Cell Biology; flow-cytometry; inducers; inhibition; interferon; living cells; Localization; Mitochondria; nucleic-acids; protein-synthesis; rhodamine-123
Jamison J M; Krabill K; Hatwalkar A; Jamison E; Tsai C C
Cell Biology International Reports
1990
1990-12
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0309-1651(90)90015-q" target="_blank" rel="noreferrer noopener">10.1016/0309-1651(90)90015-q</a>
Rna-intercalating Agent Interactions: In Vitro Antiviral Activity Studies
Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Virology
Twenty intercalating agents were tested to examine the effects of intercalating dye-induced perturbations upon the antiviral activity of poly (adenylate-uridylate) [poly (A-U)]. Neither poly (A-U) alone nor each intercalative dye was an efficacious antiviral agent. When poly (A-U) was combined with major groove intercalating dyes (acridine orange or proflavine), no synergism was observed. When poly (A-U) was combined with minor groove intercalating dyes [ethidium (EB), propidium (PI), adriamycin (ADR) or daunomycin (DMN)] or minor/major groove intercalating dyes [9-aminoacridine (9-AA), N-2-methyl-9-hydroxy-ellipticine (NMHE) or N-2,N-6-dimethyl-9-hydroxy-ellipticine (DMHE)] the 50% effective doses (ED50) of the poly (A-U), 9-AA, ADR, DMHE, DMN, EB, NMHE and PI decreased 18-, 22-, 60-, 274-, 61-, 154-, 113- and 299-fold, respectively. When poly (A-U) was combined individually with 11 dyes whose mode of intercalation was not known, the ED50 of ametantrone (HAQ), chloroquine (CHL), mitoxantrone (DHAQ) and quinine (QUI) decreased 125-, 65-, 251- and 32-fold, respectively. These results suggest that the four dyes may intercalate into poly (A-U) from the minor groove. Ten (ADR, CHL, DMN, DHAQ, DMHE, EB, HAQ, NMHE, PI, QUI) of the 20 dyes evaluated exhibited significant synergism with poly (A-U), as quantified by the fractional inhibitory concentration index. Interferon (IFN) neutralization assays demonstrated that the IFN-inducing capability of the dye/poly (A-U) combinations approximated the sum of the capabilities of the poly (A-U) and the dyes employed. These results suggest that the majority of the dyes tested potentiate the antiviral activity of poly (A-U) without affecting the amount of IFN induced.
Jamison J M; Krabill K; Allen K A; Stuart S H; Tsai C C
Antiviral Chemistry & Chemotherapy
1990
1990-12
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1177/095632029000100601" target="_blank" rel="noreferrer noopener">10.1177/095632029000100601</a>
The activity of Mg2+ and poly r(A-U) against HIV-I
Pharmacology & Pharmacy; Virology
Nassiri M R; Jamison J M; Gilloteaux J; Tsai C C; Summers J L
Antiviral Research
2000
2000-04
Journal Article
n/a