Identification of domains of AGS1 (Dexras1) required for activation of heterotrimeric Gi
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Cismowski M J; Lizano J S; Vaidyanathan G; Yang Q; Lanier S M
Faseb Journal
2005
2005-03
Journal Article or Conference Abstract Publication
n/a
The influence of EGF, PM1 mutations and posttranslational processing on the subcellular location of AGS1/DexRas1
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Struckhoff A P; Cismowski M J; Vaidyanathan G; Lanier S M
Faseb Journal
2006
2006-03
Journal Article
n/a
The Ras-related protein AGS1/RASD1 suppresses cell growth
activation; AGS1; apoptosis; binding protein; Biochemistry & Molecular Biology; cancer; Cell Biology; coupled receptors; Dexras1; Dexras1; G protein; gene; Genetics &; Heredity; hormone; identification; Integration; Oncology; RASD1; signal-transduction
AGS1/RASD1 is a Ras-related protein identified as a dexamethasone-inducible cDNA and as a signal regulator in various functional and protein-interaction screens. As an initial approach to define the role of AGS1/RASD1 as a Ras-family member, we determined its influence on cell growth/survival. In clonogenic assays with NIH-3T3 murine fibroblast cells, the MCF-7 human breast cancer cell line and the human lung adenocarcinoma cell line A549, AGS1/RASD1 markedly diminished the number of G418-resistant colonies, whereas the Ras subgroup member K-Ras was without effect. A549 cell infection with adenovirus engineered to express AGS1/RASD1 (Ad.AGS1) inhibited log phase growth in vitro and increased the percentage of cells undergoing apoptosis. The anti-growth action was also observed in vivo as the expression of AGS1/RASD1 inhibited the subcutaneous tumor growth of A549 cells in athymic nude mice. These data indicate that AGS1/RASD1, a member of the Ras superfamily of small G-proteins that often promotes cell growth and tumor expansion, plays an active role in preventing aberrant cell growth.
Vaidyanathan G; Cismowski M J; Wang G S; Vincent T S; Brown K D; Lanier S M
Oncogene
2004
2004-07
Journal Article
<a href="http://doi.org/10.1038/sj.onc.1207774" target="_blank" rel="noreferrer noopener">10.1038/sj.onc.1207774</a>