1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1210/endo.131.2.1639033" target="_blank" rel="noreferrer noopener">http://doi.org/10.1210/endo.131.2.1639033</a> Pages 964–969 Issue 2 Volume 131 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Quantification of vasoactive intestinal peptide immunoreactivity in the anterior pituitary glands of intact male and female, ovariectomized, and estradiol benzoate-treated rats. Publisher An entity responsible for making the resource available Endocrinology Date A point or period of time associated with an event in the lifecycle of the resource 1992 1992-08 Subject The topic of the resource *Ovariectomy; Animals; Anterior/*chemistry/drug effects/metabolism; Diestrus; Estradiol/*pharmacology; Female; Immunohistochemistry; Inbred Strains; Male; Pituitary Gland; Prolactin/blood/metabolism; Rats; Vasoactive Intestinal Peptide/*analysis/metabolism Creator An entity primarily responsible for making the resource Carrillo A J; Phelps C J Description An account of the resource There are considerable data suggesting that vasoactive intestinal peptide (VIP) is involved in the regulation of PRL secretion; however, the role and cell of origin of anterior pituitary VIP remain to be determined. Immunocytochemical (ICC) studies have generally failed to detect VIP-immunoreactive (IR) cells in the pituitary of the untreated rat, although VIP-IR cells have been observed in the pituitaries of hypothyroid or estrogen-treated rats. This study was designed to examine the cellular distribution and tissue content of VIP in the anterior pituitary gland of rats under selected endocrine conditions known to alter the rates of PRL and VIP synthesis and secretion. To this end, anterior pituitary VIP and PRL content (ICC and RIA) and serum PRL levels were determined in ovariectomized (OVX) and OVX rats 3 days after treatment with 7 or 70 micrograms estradiol benzoate (EB). For comparison, pituitary VIP and PRL content (ICC and RIA) and serum PRL levels in untreated male and diestrous female rats were determined. Immunostaining for VIP was accomplished using a newly developed primary antiserum. Significant numbers of VIP-IR cells per 5-microns section were found in the anterior pituitary glands of all animals examined (275 +/- 33 in diestrous to 481 +/- 103 cells in male rats). VIP was not colocalized with PRL in any of the pituitaries regardless of steroid treatment or sex. Furthermore, the number of VIP-IR cells per pituitary gland was not significantly correlated with sex or EB treatment. Treatment with 70 micrograms, but not 7 micrograms, EB significantly increased the pituitary content of VIP and serum PRL levels compared to those after ovariectomy. However, both EB treatments resulted in a significant increase in pituitary PRL content compared to that in untreated OVX rats. Pituitaries from male rats had several-fold more VIP and less PRL content than pituitaries from diestrous rats. These data show that 1) in contrast to previous ICC studies, VIP-IR cells are readily detected in the anterior pituitary of intact male and female and OVX as well as EB-treated rats; 2) VIP is localized to cells other than lactotrophs, regardless of the steroid background; and 3) marked changes in anterior pituitary VIP content are not accompanied by changes in VIP-IR cell number. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1210/endo.131.2.1639033" target="_blank" rel="noreferrer noopener">10.1210/endo.131.2.1639033</a> Rights Information about rights held in and over the resource Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). *Ovariectomy 1992 Animals Anterior/*chemistry/drug effects/metabolism Carrillo A J Diestrus Endocrinology Estradiol/*pharmacology Female Immunohistochemistry Inbred Strains Male Phelps C J Pituitary Gland Prolactin/blood/metabolism Rats Vasoactive Intestinal Peptide/*analysis/metabolism