1
40
2
-
Text
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URL Address
<a href="http://doi.org/10.1128/jvi.77.6.3409-3417.2003" target="_blank" rel="noreferrer noopener">http://doi.org/10.1128/jvi.77.6.3409-3417.2003</a>
Pages
3409–3417
Issue
6
Volume
77
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Strain-dependent structural variants of herpes simplex virus type 1 ICP34.5 determine viral plaque size, efficiency of glycoprotein processing, and viral release and neuroinvasive disease potential.
Publisher
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Journal of virology
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-03
Subject
The topic of the resource
*Genetic Variation; Animals; Cercopithecus aethiops; Cultured; Encephalitis; Genetic; Herpes Simplex/*physiopathology/virology; Herpesvirus 1; Human/*classification/genetics/*pathogenicity; Inbred BALB C; Male; Mice; Recombination; Transfection; Tumor Cells; Vero Cells; Viral Envelope Proteins/metabolism; Viral Plaque Assay; Viral Proteins/*chemistry/*genetics/metabolism
Creator
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Mao Hanwen; Rosenthal Ken S
Description
An account of the resource
The ability of certain strains of herpes simplex virus type 1 (HSV-1) to cause encephalitis or neuroinvasive disease in the mouse upon peripheral infection is dependent on a combination of activities of specific forms of viral proteins. The importance of specific variants of ICP34.5 to neuroinvasive disease potential and its correlation with small-plaque production, inefficient glycoprotein processing, and virus release were suggested by comparison of ICP34.5 from the SP7 virus, originally obtained from the brain of a neonate with disseminated disease, and the tissue culture-passaged progeny of SP7 (SLP5 and SLP10) and the KOS321 virus. SLP5, SLP10, and KOS321 are attenuated and exhibit a large-plaque phenotype, including efficient glycoprotein processing and viral release. We show that expression of the KOS321 ICP34.5 protein in cells infected with SP7 or ICP34.5 deletion mutants promotes large plaque formation and efficient viral glycoprotein processing, while expression of the SP7 ICP34.5 protein decreases efficiency of viral glycoprotein processing. In addition, a recombinant virus, 4hS1, with the SP7 ICP34.5 gene replacing the KOS321-like ICP34.5 gene in the SLP10a background, rescues the small-plaque phenotype and neuroinvasive disease. The major difference in the ICP34.5 gene product is the number of Pro-Ala-Thr repeats in the middle region of the protein, with 18 for SP7 and 3 for KOS321. Strain-dependent differences in the ICP34.5 protein can therefore alter the tissue culture behavior and the virulence of HSV-1.
Identifier
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<a href="http://doi.org/10.1128/jvi.77.6.3409-3417.2003" target="_blank" rel="noreferrer noopener">10.1128/jvi.77.6.3409-3417.2003</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Genetic Variation
2003
Animals
Cercopithecus aethiops
Cultured
Encephalitis
Genetic
Herpes Simplex/*physiopathology/virology
Herpesvirus 1
Human/*classification/genetics/*pathogenicity
Inbred BALB C
Journal of virology
Male
Mao Hanwen
Mice
Recombination
Rosenthal Ken S
Transfection
Tumor Cells
Vero Cells
Viral Envelope Proteins/metabolism
Viral Plaque Assay
Viral Proteins/*chemistry/*genetics/metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/viro.2000.0338" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/viro.2000.0338</a>
Pages
227–233
Issue
2
Volume
271
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Neural stem cells as tools for understanding retroviral neuropathogenesis.
Publisher
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Virology
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-06
Subject
The topic of the resource
Animals; Humans; Membrane Fusion/physiology; Microglia/virology; Neurons/*virology; Retroviridae Infections/virology; Retroviridae Proteins/metabolism; Retroviridae/*pathogenicity; Stem Cells/*virology; Viral Envelope Proteins/metabolism; Virulence
Creator
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Lynch W P; Portis J L
Description
An account of the resource
The discovery within the past decade that neural stem cells (NSCs) from the developing and adult mammalian brain can be propagated, cloned, and genetically manipulated ex vivo for ultimate transfer back into the CNS has opened the door to a novel means for modifying the CNS environment for experimental and therapeutic purposes. While a great deal of interest has been focused on the properties and promise of this new technology, especially in regard to cellular replacement and gene therapy, this minireview will focus on the recent use of NSCs to study the neuropathogenesis of the murine oncornaviruses. In brief, the use of this NSC-based approach has provided a means for selective reconstitution within the brain, of specific retroviral life cycle events, in order to consider their contribution to the induction of neurodegeneration. Furthermore, by virtue of their ability to disseminate virus within the brain, NSCs have provided a reliable means for assessing the true neurovirulence potential of murine oncornaviruses by directly circumventing a restriction to virus entry into the CNS. Importantly, these experiments have demonstrated that the neurovirulence of oncornaviruses requires late virus life cycle events occurring specifically within microglia, the resident macrophages of the brain. This initial application of NSC biology to the analysis of oncornavirus-CNS interactions may serve as an example for how other questions in viral neuropathogenesis might be addressed in the future.
Identifier
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<a href="http://doi.org/10.1006/viro.2000.0338" target="_blank" rel="noreferrer noopener">10.1006/viro.2000.0338</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2000
Animals
Humans
Lynch W P
Membrane Fusion/physiology
Microglia/virology
Neurons/*virology
Portis J L
Retroviridae Infections/virology
Retroviridae Proteins/metabolism
Retroviridae/*pathogenicity
Stem Cells/*virology
Viral Envelope Proteins/metabolism
Virology
Virulence