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Text
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URL Address
<a href="http://doi.org/10.1007/978-1-4939-7407-8_15" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/978-1-4939-7407-8_15</a>
Pages
171–185
Volume
1695
Dublin Core
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Title
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Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma.
Publisher
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Methods in molecular biology (Clifton, N.J.)
Date
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2018
1905-07
Subject
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*Axonal transport; *Axonopathy; *Neuronal tracing; *Optic nerve; *Superior colliculus; Animal; Animals; Axonal Transport; Axons/metabolism/*pathology/physiology; Cholera Toxin/*metabolism; Confocal; Disease Models; Glaucoma/*diagnostic imaging/metabolism/physiopathology; Humans; Mice; Microscopy; Rats; Visual Pathways
Creator
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Crish Samuel D; Schofield Brett R
Description
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Whether to stage degeneration or investigate early pathology in glaucoma, examination of axonal structure and function is essential. There are a wide variety of methods available to investigators using animal models of glaucoma, with varying utilities depending on the questions asked. Here, we describe the use of anterograde neuronal tract tracing using cholera toxin B (CTB) for the determination of axon transport integrity of the retinofugal projection. This method reveals the structure of the retinal axons as well as the functional integrity of anterograde transport systems.
Identifier
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<a href="http://doi.org/10.1007/978-1-4939-7407-8_15" target="_blank" rel="noreferrer noopener">10.1007/978-1-4939-7407-8_15</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Axonal transport
*Axonopathy
*Neuronal tracing
*Optic nerve
*superior colliculus
2018
Animal
Animals
Axonal Transport
Axons/metabolism/*pathology/physiology
Cholera Toxin/*metabolism
Confocal
Crish Samuel D
Department of Anatomy & Neurobiology
Department of Pharmaceutical Sciences
Disease Models
Glaucoma/*diagnostic imaging/metabolism/physiopathology
Humans
Methods in molecular biology (Clifton, N.J.)
Mice
Microscopy
NEOMED College of Medicine
NEOMED College of Pharmacy
Rats
Schofield Brett R
Visual Pathways