1
40
9
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/geno.1994.1177" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/geno.1994.1177</a>
Pages
320–323
Issue
2
Volume
20
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Structure and nucleotide sequences of the human cholesterol 7 alpha-hydroxylase gene (CYP7).
Publisher
An entity responsible for making the resource available
Genomics
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-03
Subject
The topic of the resource
Amino Acid Sequence; Base Sequence; Cholesterol 7-alpha-Hydroxylase/chemistry/*genetics; DNA; Humans; Molecular Sequence Data; Restriction Mapping
Creator
An entity primarily responsible for making the resource
Wang D P; Chiang J Y
Description
An account of the resource
The human cholesterol 7 alpha-hydroxylase gene (CYP7) spans about 11 kb of the genome and contains six exons and five introns. Nucleotide sequences of a 5'-upstream region to exon III (5535 bp), a 5'-upstream EcoRI fragment (2575 bp), and an EcoRI fragment covering intron V to exon VI (2319 bp) have been determined. A comparison of our sequences with those reported previously unveiled numerous sequencing discrepancies that are apparently due to sequencing errors. There are only one confirmed and one possible genetic polymorphism in the promoter of this highly conserved human gene. The proximal promoter contained many consensus recognition sequences for liver-enriched transcription factors and steroid hormone receptors that may play important roles in regulation of the CYP7 gene transcription by bile acids, cholesterol, and hormones.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/geno.1994.1177" target="_blank" rel="noreferrer noopener">10.1006/geno.1994.1177</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1994
Amino Acid Sequence
Base Sequence
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/chemistry/*genetics
Department of Integrative Medical Sciences
DNA
Genomics
Humans
Molecular Sequence Data
NEOMED College of Medicine
Restriction Mapping
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
337–339
Issue
3
Volume
1132
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Cloning and 5'-flanking sequence of a rat cholesterol 7 alpha-hydroxylase gene.
Publisher
An entity responsible for making the resource available
Biochimica et biophysica acta
Date
A point or period of time associated with an event in the lifecycle of the resource
1992
1992-10
Subject
The topic of the resource
Animals; Rats; Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Cholesterol 7-alpha-Hydroxylase/*genetics; DNA; Restriction Mapping; Genetic; Cloning; Molecular; *Promoter Regions
Creator
An entity primarily responsible for making the resource
Chiang J Y; Yang T P; Wang D P
Description
An account of the resource
A cholesterol 7 alpha-hydroxylase gene containing 8 kb of the 5'-flanking region and 5 kb of the transcription region which covers exons 1 to 5 was isolated from a rat genomic library. The 2015 bp nucleotide sequence 5'-upstream from the start codon was determined. This promotor region contains many liver-enriched or -specific elements (TGT3, HNF/LF-B1), putative hormone responsive elements (TRE, GRE, RRE or RARE) and ubiquitous transcription factor binding sites (NF-1,
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
1992
Amino Acid Sequence
Animals
Base Sequence
Biochimica et biophysica acta
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cloning
Department of Integrative Medical Sciences
DNA
Genetic
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Rats
Restriction Mapping
Wang D P
Yang T P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
12012–12019
Issue
20
Volume
265
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of cholesterol 7 alpha-hydroxylase in the liver. Cloning, sequencing, and regulation of cholesterol 7 alpha-hydroxylase mRNA.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1990
1990-07
Subject
The topic of the resource
Female; Animals; Rats; Amino Acid Sequence; *Gene Expression Regulation; Kinetics; Base Sequence; Immunoblotting; Molecular Sequence Data; Steroid Hydroxylases/*genetics; Circadian Rhythm; DNA/genetics/isolation & purification; Restriction Mapping; Enzyme Induction; Liver/drug effects/*enzymology; Cell Fractionation; Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics/immunology; Cholestyramine Resin/pharmacology; Cytochrome P-450 Enzyme System/genetics; Epitopes/analysis; Polyribosomes/metabolism/ultrastructure; Inbred Strains; RNA; Enzymologic; Sequence Homology; Cloning; Nucleic Acid; Messenger/*genetics; Centrifugation; Density Gradient; Molecular/methods
Creator
An entity primarily responsible for making the resource
Li Y C; Wang D P; Chiang J Y
Description
An account of the resource
Monospecific antibody against purified rat liver cholesterol 7 alpha-hydroxylase cytochrome P-450 was used to screen a lambda gt11 cDNA library constructed from immuno-enriched polysomal RNA of cholestyramine-treated female rat liver. Two types of cDNA clones differing in the length of the 3'-untranslated region were identified, and DNA sequences were determined. The full length clone contains 3561 base pairs plus a long poly(A) tail. The amino acid sequence deduced from the open reading frame revealed a unique P-450 protein containing 503 amino acid residues which belonged to a new gene family designated family VII or CYP7. Southern blot hybridization experiments indicated that the minimal size of P-450 VII gene was 11 kilobase pairs (kb), and there was probably only one gene in this new family. Northern blot hybridization using specific cDNA probes revealed at least two major mRNA species of about 4.0 kb and 2.1 kb, respectively. These two mRNA species may be derived from the use of different polyadenylation signals and reverse-transcribed to two types of cDNA clones. Cholesterol 7 alpha-hydroxylase mRNAs were induced 2- to 3-fold in rat liver by cholestyramine treatment. The mRNA level was rapidly reduced upon the removal of the inducer. Similarly, cholesterol feeding induced enzyme activity, protein, and mRNA levels in the rat by 2-fold, suggesting that cholesterol is an important regulator of cholesterol 7 alpha-hydroxylase in the liver. On the other hand, dexamethasone and pregnenolone-16 alpha-carbonitrile drastically reduced the activity, protein, and mRNA levels. These experiments suggest that the induction of cholesterol 7 alpha-hydroxylase activity by cholestyramine or cholesterol and inhibition of cholesterol 7 alpha-hydroxylase activity by bile acid feedback are results of the rapid turnover of cholesterol 7 alpha-hydroxylase enzyme and mRNA levels.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
1990
Amino Acid Sequence
Animals
Base Sequence
Cell Fractionation
Centrifugation
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics/immunology
Cholestyramine Resin/pharmacology
Circadian Rhythm
Cloning
Cytochrome P-450 Enzyme System/genetics
Density Gradient
Department of Integrative Medical Sciences
DNA/genetics/isolation & purification
Enzyme Induction
Enzymologic
Epitopes/analysis
Female
Immunoblotting
Inbred Strains
Kinetics
Li Y C
Liver/drug effects/*enzymology
Messenger/*genetics
Molecular Sequence Data
Molecular/methods
NEOMED College of Medicine
Nucleic Acid
Polyribosomes/metabolism/ultrastructure
Rats
Restriction Mapping
RNA
Sequence Homology
Steroid Hydroxylases/*genetics
The Journal of biological chemistry
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
1831–1841
Issue
9
Volume
37
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene (CYP7A) in HepG2 cells.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-09
Subject
The topic of the resource
Humans; Binding Sites; Gene Expression Regulation; Cell Line; Transfection; Base Sequence; Molecular Sequence Data; Phorbol Esters/pharmacology; DNA-Binding Proteins/genetics/metabolism; *Transcription Factors; Enzyme Repression; Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics; Consensus Sequence; Glucocorticoids/pharmacology; Hepatocyte Nuclear Factor 3-alpha; Insulin/pharmacology; Nuclear Proteins/genetics/metabolism; Recombinant Fusion Proteins/biosynthesis; Thyroid Hormones/pharmacology; Genes; Receptors; Enzymologic/*drug effects; Genetic; *Promoter Regions; Reporter; *Transcription; Glucocorticoid/genetics/metabolism
Creator
An entity primarily responsible for making the resource
Wang D P; Stroup D; Marrapodi M; Crestani M; Galli G; Chiang J Y
Description
An account of the resource
A stable HepG2 cell line harboring a human cholesterol 7 alpha-hydroxylase (CYP7A) minigene/luciferase reporter gene construct was selected for studying transcriptional regulation of CYP7A gene promoter. Insulin and phorbol
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
*Transcription
*Transcription Factors
1996
Base Sequence
Binding Sites
Cell Line
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics
Consensus Sequence
Crestani M
Department of Integrative Medical Sciences
DNA-Binding Proteins/genetics/metabolism
Enzyme Repression
Enzymologic/*drug effects
Galli G
Gene Expression Regulation
Genes
Genetic
Glucocorticoid/genetics/metabolism
Glucocorticoids/pharmacology
Hepatocyte Nuclear Factor 3-alpha
Humans
Insulin/pharmacology
Journal of lipid research
Marrapodi M
Molecular Sequence Data
NEOMED College of Medicine
Nuclear Proteins/genetics/metabolism
Phorbol Esters/pharmacology
Receptors
Recombinant Fusion Proteins/biosynthesis
Reporter
Stroup D
Thyroid Hormones/pharmacology
Transfection
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A1383-A1383
Issue
7
Volume
8
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
TRANSCRIPTIONAL REGULATION OF HUMAN CHOLESTEROL 7-ALPHA-HYDROXYLASE GENE IN HEPG2 CELLS
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-04
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Wang D P; Karam W G; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article
1994
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Faseb Journal
Journal Article
Karam W G
Life Sciences & Biomedicine - Other
Topics
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Volume
2
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
THE EXPRESSION AND REGULATION OF CHOLESTEROL 7-ALPHA-HYDROXYLASE
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Karam W G; Yang T P; Wang D P
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Book/Monograph
1993
Book/Monograph
Chiang J Y L
Karam W G
Wang D P
Yang T P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Volume
80
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Molecular mechanism of regulation of cholesterol 7 alpha-hydroxylase gene
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Stroup D; Crestani M; Wang D P
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Book/Monograph
1995
Book/Monograph
Chiang J Y L
Crestani M
Stroup D
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
2865-2865
Issue
6
Volume
10
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional Regulation Of The Human Cholesterol 7 Alpha-hydroxylase Gene (cyp7a) In Transiently And Stably Transfected Hepg2 Cells
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-04
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Marrapodi M; Wang D P; Stroup D; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1996
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Faseb Journal
Life Sciences & Biomedicine - Other
Marrapodi M
Stroup D
Topics
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
12012-12019
Issue
20
Volume
265
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation Of Cholesterol 7-alpha-hydroxylase In The Liver - Cloning, Sequencing, And Regulation Of Cholesterol 7-alpha-hydroxylase Messenger-rna
Publisher
An entity responsible for making the resource available
Journal of Biological Chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1990
1990-07
Subject
The topic of the resource
Biochemistry & Molecular Biology
Creator
An entity primarily responsible for making the resource
Li Y C; Wang D P; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1990
Biochemistry & Molecular Biology
Chiang J Y L
Journal Article or Conference Abstract Publication
Journal of Biological Chemistry
Li Y C
Wang D P