1
40
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajpendo.00457.2017" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajpendo.00457.2017</a>
Pages
E416–E424
Issue
3
Volume
315
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS.
Publisher
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American journal of physiology. Endocrinology and metabolism
Date
A point or period of time associated with an event in the lifecycle of the resource
2018
2018-09
Subject
The topic of the resource
dyslipidemia; insulin resistance; kinetics; lipid turnover; mass spectrometry
Creator
An entity primarily responsible for making the resource
Chen Ying; Berejnaia Olga; Liu Jinqi; Wang Sheng-Ping; Daurio Natalie A; Yin Wu; Mayoral Rafael; Petrov Aleksandr; Kasumov Takhar; Zhang Guo-Fang; Previs Stephen F; Kelley David E; McLaren David G
Description
An account of the resource
Numerous studies have implicated dyslipidemia as a key factor in mediating insulin resistance. Ceramides have received special attention since their levels are inversely associated with normal insulin signaling and positively associated with factors that are involved in cardiometabolic disease. Despite the growing literature surrounding ceramide biology, there are limited data regarding the activity of ceramide synthesis and turnover in vivo. Herein, we demonstrate the ability to measure ceramide kinetics by coupling the administration of [(2)H]water with LC-MS/MS analyses. As a "proof-of-concept" we determined the effect of a diet-induced alteration on ceramide flux; studies also examined the effect of myriocin (a known inhibitor of serine palmitoyltransferase, the first step in sphingosine biosynthesis). Our data suggest that one can estimate ceramide synthesis and draw conclusions regarding the source of fatty acids; we discuss caveats in regards to method development in this area.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/ajpendo.00457.2017" target="_blank" rel="noreferrer noopener">10.1152/ajpendo.00457.2017</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2018
American journal of physiology. Endocrinology and metabolism
Berejnaia Olga
Chen Ying
Daurio Natalie A
Department of Pharmaceutical Sciences
dyslipidemia
Insulin Resistance
Kasumov Takhar
Kelley David E
Kinetics
lipid turnover
Liu Jinqi
Mass spectrometry
Mayoral Rafael
McLaren David G
NEOMED College of Pharmacy
Petrov Aleksandr
Previs Stephen F
Wang Sheng-Ping
Yin Wu
Zhang Guo-Fang
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1124/jpet.117.241091" target="_blank" rel="noreferrer noopener">http://doi.org/10.1124/jpet.117.241091</a>
Pages
80–91
Issue
1
Volume
363
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Enhancing Studies of Pharmacodynamic Mechanisms via Measurements of Metabolic Flux: Fundamental Concepts and Guiding Principles for Using Stable Isotope Tracers.
Publisher
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The Journal of pharmacology and experimental therapeutics
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-10
Subject
The topic of the resource
Animals; Drug Discovery/*methods; Humans; Isotope Labeling; Isotopes/chemistry; Metabolic Flux Analysis/*methods; Water/chemistry/metabolism
Creator
An entity primarily responsible for making the resource
Daurio Natalie A; Wang Sheng-Ping; Chen Ying; Zhou Haihong; McLaren David G; Roddy Thomas P; Johns Douglas G; Milot Denise; Kasumov Takhar; Erion Mark D; Kelley David E; Previs Stephen F
Description
An account of the resource
Drug discovery and development efforts are largely based around a common expectation, namely, that direct or indirect action on a cellular process (e.g., statin-mediated enzyme inhibition or insulin-stimulated receptor activation) will have a beneficial impact on physiologic homeostasis. To expand on this, one could argue that virtually all pharmacologic interventions attempt to influence the flow of "traffic" in a biochemical network, irrespective of disease or modality. Since stable isotope tracer kinetic methods provide a measure of traffic flow (i.e., metabolic flux), their inclusion in study designs can yield novel information regarding pathway biology; the application of such methods requires the integration of knowledge in physiology, analytical chemistry, and mathematical modeling. Herein, we review the fundamental concepts that surround the use of tracer kinetics, define basic terms, and outline guiding principles via theoretical and experimental problems. Specifically, one needs to 1) recognize the types of biochemical events that change isotopic enrichments, 2) appreciate the distinction between fractional turnover and flux rate, and 3) be aware of the subtle differences between tracer kinetics and pharmacokinetics. We hope investigators can use the framework presented here to develop applications that address their specific questions surrounding biochemical flux, and thereby gain insight into the pathophysiology of disease states, and examine pharmacodynamic mechanisms.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1124/jpet.117.241091" target="_blank" rel="noreferrer noopener">10.1124/jpet.117.241091</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Animals
Chen Ying
Daurio Natalie A
Department of Pharmaceutical Sciences
Drug Discovery/*methods
Erion Mark D
Humans
Isotope Labeling
Isotopes/chemistry
Johns Douglas G
Kasumov Takhar
Kelley David E
McLaren David G
Metabolic Flux Analysis/*methods
Milot Denise
NEOMED College of Pharmacy
Previs Stephen F
Roddy Thomas P
The Journal of pharmacology and experimental therapeutics
Wang Sheng-Ping
Water/chemistry/metabolism
Zhou Haihong