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Text
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URL Address
<a href="http://doi.org/10.1007/bf00420948" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/bf00420948</a>
Pages
615–618
Issue
6
Volume
92
Dublin Core
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Title
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The human cytochrome b5 gene and two of its pseudogenes are located on chromosomes 18q23, 14q31-32.1 and 20p11.2, respectively.
Publisher
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Human genetics
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-12
Subject
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*Chromosomes; Animals; Blotting; Cell Line; Chromosome Mapping; Cricetinae; Cytochromes b5/*genetics; DNA/analysis; Genetic/genetics; Human; Humans; Hybrid Cells; In Situ Hybridization; Molecular Sequence Data; Pair 14; Pair 18; Pair 20; Polymerase Chain Reaction; Pseudogenes/*genetics; Southern; Translocation
Creator
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Giordano S J; Yoo M; Ward D C; Bhatt M; Overhauser J; Steggles A W
Description
An account of the resource
Using very high stringency hybridization conditions for the Southern blot hybridization analysis of hamster-human cell hybrid DNA, we were able to map the human cytochrome b5 gene and two of its pseudogenes (psgb(5)1 and psgb(5)2) unambiguously to chromosomes 18, 14, and 20. These localizations were confirmed and extended to 18q23, 14q31-32.1, and 20p11.2 by using a combination of nonisotopic in situ hybridization of chromosomal spreads and the polymerase chain reaction analysis of DNA samples isolated from somatic cell hybrids retaining deletions or translocations of chromosome 18.
Identifier
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<a href="http://doi.org/10.1007/bf00420948" target="_blank" rel="noreferrer noopener">10.1007/bf00420948</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Chromosomes
1993
Animals
Bhatt M
Blotting
Cell Line
Chromosome Mapping
Cricetinae
Cytochromes b5/*genetics
DNA/analysis
Genetic/genetics
Giordano S J
Human
Human genetics
Humans
Hybrid Cells
In Situ Hybridization
Molecular Sequence Data
Overhauser J
Pair 14
Pair 18
Pair 20
Polymerase Chain Reaction
Pseudogenes/*genetics
Southern
Steggles A W
Translocation
Ward D C
Yoo M