1
40
27
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1155/2013/371813" target="_blank" rel="noreferrer noopener">http://doi.org/10.1155/2013/371813</a>
Pages
1–15
Volume
2013
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Pomegranate Bioactive Constituents Suppress Cell Proliferation and Induce Apoptosis in an Experimental Model of Hepatocellular Carcinoma: Role of Wnt/β-Catenin Signaling Pathway.
Publisher
An entity responsible for making the resource available
Evidence-based Complementary & Alternative Medicine (eCAM)
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-01
Subject
The topic of the resource
Immunohistochemistry; Gene Expression; Apoptosis; Rats; Pomegranate; Post Hoc Analysis; One-Way Analysis of Variance; Structural Equation Modeling; Experimental Studies; Protocols; Carcinoma; Blotting; Western; Hepatocellular; Animal Studies; In Vivo Studies
Creator
An entity primarily responsible for making the resource
Bhatia Deepak; Thoppil Roslin J; Mandal Animesh; Samtani Karishma A; Darvesh Altaf S; Bishayee Anupam
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1155/2013/371813" target="_blank" rel="noreferrer noopener">10.1155/2013/371813</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2013
Animal Studies
Apoptosis
Bhatia Deepak
Bishayee Anupam
Blotting
Carcinoma
Darvesh Altaf S
Department of Pharmaceutical Sciences
Evidence-based Complementary & Alternative Medicine (eCAM)
Experimental Studies
Gene Expression
Hepatocellular
Immunohistochemistry
In Vivo Studies
Mandal Animesh
NEOMED College of Pharmacy
One-Way Analysis of Variance
Pomegranate
Post Hoc Analysis
Protocols
Rats
Samtani Karishma A
STRUCTURAL equation modeling
Thoppil Roslin J
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1155/2014/769515" target="_blank" rel="noreferrer noopener">http://doi.org/10.1155/2014/769515</a>
Pages
1–9
Volume
2014
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Apocynum Tablet Protects against Cardiac Hypertrophy via Inhibiting AKT and ERK1/2 Phosphorylation after Pressure Overload.
Publisher
An entity responsible for making the resource available
Evidence-based Complementary & Alternative Medicine (eCAM)
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-01
Subject
The topic of the resource
Mice; Gene Expression Profiling; Signal Transduction; Echocardiography; Staining and Labeling; Descriptive Statistics; Funding Source; Post Hoc Analysis; One-Way Analysis of Variance; T-Tests; Blotting; Western; Animal Studies; In Vivo Studies; Vasoconstriction; Phosphorylation – Drug Effects; Aorta – Physiopathology; Heart Diseases – Prevention and Control; Hypertrophy – Prevention and Control; Plant Extracts – Therapeutic Use; Protein Kinases – Antagonists and Inhibitors
Creator
An entity primarily responsible for making the resource
Qi Jianyong; Liu Qin; Gong Kaizheng; Yu Juan; Wang Lei; Guo Liheng; Zhou Miao; Wu Jiashin; Zhang Minzhou
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1155/2014/769515" target="_blank" rel="noreferrer noopener">10.1155/2014/769515</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2014
Animal Studies
Aorta – Physiopathology
Blotting
Descriptive Statistics
Echocardiography
Evidence-based Complementary & Alternative Medicine (eCAM)
Funding Source
Gene Expression Profiling
Gong Kaizheng
Guo Liheng
Heart Diseases – Prevention and Control
Hypertrophy – Prevention and Control
In Vivo Studies
Liu Qin
Mice
One-Way Analysis of Variance
Phosphorylation – Drug Effects
Plant Extracts – Therapeutic Use
Post Hoc Analysis
Protein Kinases – Antagonists and Inhibitors
Qi Jianyong
Signal Transduction
Staining and Labeling
T-Tests
Vasoconstriction
Wang Lei
Western
Wu Jiashin
Yu Juan
Zhang Minzhou
Zhou Miao
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
19186–19191
Issue
29
Volume
266
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The expression of a catalytically active cholesterol 7 alpha-hydroxylase cytochrome P450 in Escherichia coli.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1991
1991-10
Subject
The topic of the resource
Gene Expression; Amino Acid Sequence; Base Sequence; Polymerase Chain Reaction; Liver/enzymology; Catalysis; Molecular Sequence Data; Substrate Specificity; Cholesterol/metabolism; Cholesterol 7-alpha-Hydroxylase/*genetics; DNA/genetics; Codon; Escherichia coli/*enzymology; Plasmids; Chromatography; Blotting; Western; Electrophoresis; Polyacrylamide Gel; Liquid; Microsomes
Creator
An entity primarily responsible for making the resource
Li Y C; Chiang J Y
Description
An account of the resource
We have recently cloned a full-length cDNA encoding the rat hepatic cholesterol 7 alpha-hydroxylase cytochrome P450 (P450c7) (Li, Y. C., Wang, D. P., and Chiang, J. Y. L. (1990) J. Biol. Chem. 265, 12012-12019), which catalyzes the rate-limiting reaction of bile acid synthesis in the liver. By using the polymerase chain reaction, we have designed two P450c7 cDNAs. One has the second Met codon deleted and the third Thr codon replaced with an Ala. The other lacks codons for the NH2-terminal hydrophobic sequence of amino acids 2-24 (P450c7 delta 2-24). The cDNAs were separately cloned into the expression vector pKK233-2 and transformed into Escherichia coli. After induction with isopropyl-beta-D-thiogalactopyranoside, bacteria harboring recombinant plasmids expressed a polypeptide which reacted with the antibody against cholesterol 7 alpha-hydroxylase in immunoblots. The slightly modified full-length enzyme was expressed to 0.2% of the total bacterial lysate and was located in the membrane fraction, whereas P450c7 delta 2-24 was expressed at a 10-fold higher level (2%), of which 85% was in the cytosol and the remaining associated with the membranes. We have purified P450c7 delta 2-24 which showed a typical reduced-CO difference spectrum of cytochrome P450 and reconstituted cholesterol 7 alpha-hydroxylase activity in the presence of NADPH-cytochrome P450 reductase. P450c7 delta 2-24 has a similar Km for cholesterol (24.6 microM) but a lower Vmax (0.10 nmol/min) and a lower turnover number (1.93 min-1) as compared with the enzyme isolated from rat liver microsomes. The purified P450c7 delta 2-24 has an unique hydrophilic NH2 terminus and contains monomers and dimers in equal amounts. This is the first report demonstrating that a genetically engineered cytochrome P450 enzyme lacking a typical NH2-terminal hydrophobic sequence is mainly cytosolic and catalytically active.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1991
Amino Acid Sequence
Base Sequence
Blotting
Catalysis
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cholesterol/metabolism
Chromatography
Codon
Department of Integrative Medical Sciences
DNA/genetics
Electrophoresis
Escherichia coli/*enzymology
Gene Expression
Li Y C
Liquid
Liver/enzymology
Microsomes
Molecular Sequence Data
NEOMED College of Medicine
Plasmids
Polyacrylamide Gel
Polymerase Chain Reaction
Substrate Specificity
The Journal of biological chemistry
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.2174/156800912803987968" target="_blank" rel="noreferrer noopener">http://doi.org/10.2174/156800912803987968</a>
Pages
1244–1257
Issue
9
Volume
12
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Black currant anthocyanins abrogate oxidative stress through Nrf2- mediated antioxidant mechanisms in a rat model of hepatocellular carcinoma.
Publisher
An entity responsible for making the resource available
Current cancer drug targets
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-11
Subject
The topic of the resource
Male; Animals; Rats; Diet; Signal Transduction; NF-E2-Related Factor 2/genetics/*metabolism; *Phytotherapy; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Alkylating Agents/toxicity; Anthocyanins/*therapeutic use; Antioxidants/therapeutic use; Diethylnitrosamine/toxicity; Glutathione Transferase; Immunoenzyme Techniques; Lipid Peroxidation/drug effects; Nitric Oxide Synthase Type II/genetics/metabolism; Oxidative Stress/*drug effects; Ribes/*chemistry; Tyrosine/analogs & derivatives/metabolism; Carcinoma; Sprague-Dawley; Blotting; Western; RNA; Liver Neoplasms; Messenger/genetics; Experimental/chemically induced/metabolism/*prevention & control; Hepatocellular/chemically induced/metabolism/*prevention & control
Creator
An entity primarily responsible for making the resource
Thoppil Roslin J; Bhatia Deepak; Barnes Kendra F; Haznagy-Radnai Erzsebet; Hohmann Judit; Darvesh Altaf S; Bishayee Anupam
Description
An account of the resource
Hepatocellular carcinoma (HCC), considered to be one of the most lethal cancers with almost \textgreater 1 million deaths reported annually worldwide, remains a devastating disease with no known effective cure. Hence, chemopreventive strategies come into play, offering an effective and safe mode of treatment, ideal to ward off potential cancer risks and mortality. A major predisposing condition, pertinent to the development and progression of HCC is oxidative stress. We previously reported a striking chemopreventive effect of anthocyanin-rich black currant skin extract (BCSE) against diethylnitrosamine (DENA)-initiated hepatocarcinogenesis in rats. The current study aims to elucidate the underlying antioxidant mechanisms of black currant anthocyanins implicated in the previously observed chemopreventive effects against experimental hepatocarcinogenesis. Dietary BCSE (100 and 500 mg/kg) administered four weeks before and 18 weeks after DENA challenge decreased abnormal lipid peroxidation, protein oxidation, and expression of inducible nitric oxide synthase (iNOS) and 3-nitrotyrosine (3-NT) in a dose-responsive fashion. Mechanistic studies revealed that BCSE upregulated the gene expression of a number of hepatic antioxidant and carcinogen detoxifying enzymes, such as NAD(P)H:quinone oxidoreductase, glutathione S-transferase, and uridine diphosphate-glucuronosyltransferase isoenzymes, in DENA-initiated animals. Protein and mRNA expressions of nuclear factor E2-related factor 2 (Nrf2) were substantially elevated with BCSE treatment, providing a direct evidence of a coordinated activation of the Nrf2-regulated antioxidant pathway, which led to the upregulation of a variety of housekeeping genes. The results of our study provide substantial evidence that black currant bioactive anthocyanins exert chemopreventive actions against DENA-inflicted hepatocarcinogenesis by attenuating oxidative stress through activation of Nrf2 signaling pathway.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.2174/156800912803987968" target="_blank" rel="noreferrer noopener">10.2174/156800912803987968</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Phytotherapy
2012
Alkylating Agents/toxicity
Animals
Anthocyanins/*therapeutic use
Antioxidants/therapeutic use
Barnes Kendra F
Bhatia Deepak
Bishayee Anupam
Blotting
Carcinoma
Current cancer drug targets
Darvesh Altaf S
Department of Pharmaceutical Sciences
Diet
Diethylnitrosamine/toxicity
Experimental/chemically induced/metabolism/*prevention & control
Glutathione Transferase
Haznagy-Radnai Erzsebet
Hepatocellular/chemically induced/metabolism/*prevention & control
Hohmann Judit
Immunoenzyme Techniques
Lipid Peroxidation/drug effects
Liver Neoplasms
Male
Messenger/genetics
NEOMED College of Pharmacy
NF-E2-Related Factor 2/genetics/*metabolism
Nitric Oxide Synthase Type II/genetics/metabolism
Oxidative Stress/*drug effects
Rats
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Ribes/*chemistry
RNA
Signal Transduction
Sprague-Dawley
Thoppil Roslin J
Tyrosine/analogs & derivatives/metabolism
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.18632/oncotarget.2676" target="_blank" rel="noreferrer noopener">http://doi.org/10.18632/oncotarget.2676</a>
Pages
1286–1301
Issue
2
Volume
6
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A class of genes in the HER2 regulon that is poised for transcription in breast cancer cell lines and expressed in human breast tumors.
Publisher
An entity responsible for making the resource available
Oncotarget
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015-01
Subject
The topic of the resource
Humans; Cell Line; *Gene Expression Regulation; Reverse Transcriptase Polymerase Chain Reaction; *Gene Expression Profiling; Breast Neoplasms/genetics/pathology; Gene Regulatory Networks; Homeodomain Proteins/genetics/metabolism; MCF-7 Cells; Nanog Homeobox Protein; Neoplastic Stem Cells/metabolism; Octamer Transcription Factor-3/genetics/metabolism; Regulon/*genetics; RNA Polymerase II/metabolism; SOXB1 Transcription Factors/genetics/metabolism; Tumor Microenvironment/genetics; Receptor; Blotting; Western; Tumor; Neoplastic; ErbB-2/*genetics/metabolism
Creator
An entity primarily responsible for making the resource
Rahmatpanah Farah B; Jia Zhenyu; Chen Xin; Char Jessica E; Men Bozhao; Franke Anna-Clara; Jones Frank E; McClelland Michael; Mercola Dan
Description
An account of the resource
HER2-positive breast cancer accounts for 25% of all cases and has a poor prognosis. Although progress has been made in understanding signal transduction, little is known of how HER2 achieves gene regulation. We performed whole genome expression analysis on a HER2(+) and HER2(-) breast cancer cell lines and compared these results to expression in 812 primary tumors stratified by their HER2 expression level. Chip-on-chip with anti-RNA polymerase II was compared among breast cancer cell lines to identify genes that are potentially activated by HER2. The expression levels of these HER2-dependent POL II binding genes were determined for the 812 HER2+/- breast cancer tissues. Genes differentially expressed between HER2+/- cell lines were generally regulated in the same direction as in breast cancer tissues. We identified genes that had POLII binding in HER2(+) cell lines, but without significant gene expression. Of 737 such genes "poised" for expression in cell lines, 113 genes were significantly differentially expressed in breast tumors in a HER2-dependent manner. Pathway analysis of these 113 genes revealed that a large group of genes were associated with stem cell and progenitor cell control as indicated by networks centered on NANOG, SOX2, OCT3/4. HER2 directs POL II binding to a large number of genes in breast cancer cells. A "poised" class of genes in HER2(+) cell lines with POLII binding and low RNA expression but is differentially expressed in primary tumors, strongly suggests a role of the microenvironment and further suggests a role for stem cells proliferation in HER2-regulated breast cancer tissue.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.18632/oncotarget.2676" target="_blank" rel="noreferrer noopener">10.18632/oncotarget.2676</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Profiling
*Gene Expression Regulation
2015
Blotting
Breast Neoplasms/genetics/pathology
Cell Line
Char Jessica E
Chen Xin
ErbB-2/*genetics/metabolism
Franke Anna-Clara
Gene Regulatory Networks
Homeodomain Proteins/genetics/metabolism
Humans
Jia Zhenyu
Jones Frank E
McClelland Michael
MCF-7 Cells
Men Bozhao
Mercola Dan
Nanog Homeobox Protein
Neoplastic
Neoplastic Stem Cells/metabolism
Octamer Transcription Factor-3/genetics/metabolism
Oncotarget
Rahmatpanah Farah B
Receptor
Regulon/*genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA Polymerase II/metabolism
SOXB1 Transcription Factors/genetics/metabolism
Tumor
Tumor Microenvironment/genetics
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1523/JNEUROSCI.4733-10.2011" target="_blank" rel="noreferrer noopener">http://doi.org/10.1523/JNEUROSCI.4733-10.2011</a>
Pages
6121–6131
Issue
16
Volume
31
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Ambient GABA-activated tonic inhibition sharpens auditory coincidence detection via a depolarizing shunting mechanism.
Publisher
An entity responsible for making the resource available
The Journal of neuroscience : the official journal of the Society for Neuroscience
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-04
Subject
The topic of the resource
Animals; Chick Embryo; Patch-Clamp Techniques; Electric Stimulation; Neurons/*physiology; gamma-Aminobutyric Acid/*physiology; Membrane Potentials/physiology; Auditory Pathways/*physiology; Neural Inhibition/*physiology; Inhibitory Postsynaptic Potentials; Receptors; Blotting; Western; GABA-A/*physiology
Creator
An entity primarily responsible for making the resource
Tang Zheng-Quan; Dinh Emilie Hoang; Shi Wei; Lu Yong
Description
An account of the resource
Tonic inhibition mediated by extrasynaptic GABA(A) receptors (GABA(A)Rs) has emerged as a novel form of neural inhibition in the CNS. However, little is known about its presence and function in the auditory system. Using whole-cell recordings in brain slices, we identified a tonic current mediated by GABA(A)Rs containing the delta subunit in middle/high-characteristic-frequency neurons of the chicken nucleus laminaris, the first interaural time difference encoder that computes information for sound localization. This tonic conductance was activated by ambient concentrations of GABA released from synaptic vesicles. Furthermore, pharmacological manipulations of the conductance demonstrated its essential role in coincidence detection. Remarkably, this depolarizing tonic conductance was strongly inhibitory primarily because of its shunting effect. These results demonstrate a novel role for tonic inhibition in central auditory information processing.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1523/JNEUROSCI.4733-10.2011" target="_blank" rel="noreferrer noopener">10.1523/JNEUROSCI.4733-10.2011</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2011
Animals
Auditory Pathways/*physiology
Blotting
Chick Embryo
Department of Anatomy & Neurobiology
Dinh Emilie Hoang
Electric Stimulation
GABA-A/*physiology
gamma-Aminobutyric Acid/*physiology
Inhibitory Postsynaptic Potentials
Lu Yong
Membrane Potentials/physiology
NEOMED College of Medicine
Neural Inhibition/*physiology
Neurons/*physiology
Patch-Clamp Techniques
Receptors
Shi Wei
Tang Zheng-Quan
The Journal of neuroscience : the official journal of the Society for Neuroscience
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajpendo.00327.2009" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajpendo.00327.2009</a>
Pages
E1147–1153
Issue
5
Volume
297
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Impact of type 1 diabetes on cardiac fibroblast activation: enhanced cell cycle progression and reduced myofibroblast content in diabetic myocardium.
Publisher
An entity responsible for making the resource available
American journal of physiology. Endocrinology and metabolism
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-11
Subject
The topic of the resource
Animals; Blood Glucose/metabolism; Blotting; Body Weight/physiology; Cell Cycle Proteins/biosynthesis; Cell Cycle/*physiology; Cell Differentiation/physiology; Cell Proliferation; Cell Separation; Diabetes Mellitus; Echocardiography; Experimental/pathology; Fibroblasts/*physiology; Male; Microarray Analysis; Myocardium/cytology/*pathology; Myofibroblasts/*physiology; Phenotype; Rats; RNA/biosynthesis/isolation & purification; Signal Transduction/physiology; Sprague-Dawley; Type 1/diagnostic imaging/*pathology; Western
Creator
An entity primarily responsible for making the resource
Shamhart Patricia E; Luther Daniel J; Hodson Ben R; Koshy John C; Ohanyan Vahagn; Meszaros J Gary
Description
An account of the resource
Diabetic patients are prone to developing myocardial fibrosis and suffer from decreased wound healing capabilities. The purpose of this study was to determine whether diabetes alters cardiac fibroblast activity in the myocardium in a 6-wk streptozotocin-induced type 1 diabetic model. In vivo echocardiography indicated significant dilation of the left ventricle (LV) in the diabetic animals, while cardiac function was comparable to that in the normal group. We isolated cardiac fibroblasts from diabetic and control hearts and observed increased proliferation of the diabetic fibroblasts. Microarray analysis using mRNA collected from whole LVs revealed downregulation of known inhibitors of proliferation, p53 and p21, in the diabetic group, consistent with our proliferation data. Western blot analysis confirmed a reduction in p53 protein expression in the diabetic hearts compared with control. We explored the potential signaling underlying the downregulation of these cell cycle mediators and determined that activated Akt, a signal that inhibits p53, was elevated in the diabetic group. Surprisingly, the hearts from the diabetic group contained lower levels of the myofibroblast marker alpha-smooth muscle actin (alpha-SMA) and higher levels of desmin and platelet endothelial cell adhesion molecule (PECAM). The isolated fibroblasts from the diabetic group also contained significantly less alpha-SMA. These data suggest that early-stage diabetic hearts contain highly proliferative fibroblasts, which predisposes the diabetic myocardium to fibrosis, but have fewer myofibroblasts, which may compromise wound healing.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/ajpendo.00327.2009" target="_blank" rel="noreferrer noopener">10.1152/ajpendo.00327.2009</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2009
American journal of physiology. Endocrinology and metabolism
Animals
Blood Glucose/metabolism
Blotting
Body Weight/physiology
Cell Cycle Proteins/biosynthesis
Cell Cycle/*physiology
Cell Differentiation/physiology
Cell Proliferation
Cell Separation
Department of Integrative Medical Sciences
Diabetes Mellitus
Echocardiography
Experimental/pathology
Fibroblasts/*physiology
Hodson Ben R
Koshy John C
Luther Daniel J
Male
Meszaros J Gary
Microarray Analysis
Myocardium/cytology/*pathology
Myofibroblasts/*physiology
NEOMED College of Medicine
Ohanyan Vahagn
Phenotype
Rats
RNA/biosynthesis/isolation & purification
Shamhart Patricia E
Signal Transduction/physiology
Sprague-Dawley
Type 1/diagnostic imaging/*pathology
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1124/jpet.104.073155" target="_blank" rel="noreferrer noopener">http://doi.org/10.1124/jpet.104.073155</a>
Pages
502–508
Issue
2
Volume
312
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Activation of metabotropic glutamate receptor 1 dimers requires glutamate binding in both subunits.
Publisher
An entity responsible for making the resource available
The Journal of pharmacology and experimental therapeutics
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-02
Subject
The topic of the resource
Blotting; Calcium Channels/drug effects/metabolism; DNA/biosynthesis/genetics; Dose-Response Relationship; Drug; Fluorescent Antibody Technique; Genes; Glutamic Acid/*metabolism; Humans; Membrane Potentials/drug effects; Metabotropic Glutamate/genetics/*metabolism; myc/genetics; Patch-Clamp Techniques; Plasmids/genetics; Receptors; Signal Transduction/drug effects; Superior Cervical Ganglion/cytology/drug effects/metabolism; Sympathetic Nervous System/cytology/drug effects/metabolism; Western
Creator
An entity primarily responsible for making the resource
Kammermeier Paul J; Yun June
Description
An account of the resource
Group I metabotropic glutamate receptors (mGluRs) form stable, disulfide-linked homodimers. Lack of a verifiably monomeric mGluR1 mutant has led to difficulty in assessing the role of dimerization in the molecular mechanism of mGluR1 activation. The related GABA(B) receptor exhibits striking intradimer cross talk (ligand binding at one subunit effectively produces G protein activation at the other), but it is unclear whether group I mGluRs exhibit analogous cross talk. Signaling of heterologously expressed mGluR1 was examined in isolated rat sympathetic neurons by measuring glutamate-mediated inhibition of native calcium currents. To examine mGluR1 activity when only one dimer subunit has access to glutamate ligand, wildtype mGluR1 was coexpressed with mGluR1 Y74A, a mutant with impaired glutamate binding, and the activity of the heterodimer (mutant/wild type) was examined. The mGluR1 Y74A mutant alone had a dose-response curve that was shifted by about 2 orders of magnitude. The half-maximal dose of glutamate shifted from 1.3 (wild-type mGluR1) to about 450 (mGluR1 Y74A) microM. However, the maximal effect was similar. Wild-type mGluR1 was expressed with excess Y74A mGluR1 to generate a receptor population consisting largely of mutant homodimers and mutant/wild-type heterodimers but without detectable wild-type homodimers. Under these conditions, no glutamate-mediated calcium current inhibition was observed below approximately 300 microM glutamate, although wild-type mGluR1 protein was detectable with immunofluorescence. These data suggest that mutant/wild-type heterodimeric receptors are inactive at ligand concentrations favoring glutamate association with receptor dimers at only one subunit.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1124/jpet.104.073155" target="_blank" rel="noreferrer noopener">10.1124/jpet.104.073155</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2005
Blotting
Calcium Channels/drug effects/metabolism
Department of Integrative Medical Sciences
DNA/biosynthesis/genetics
Dose-Response Relationship
Drug
Fluorescent Antibody Technique
Genes
Glutamic Acid/*metabolism
Humans
Kammermeier Paul J
Membrane Potentials/drug effects
Metabotropic Glutamate/genetics/*metabolism
myc/genetics
NEOMED College of Medicine
Patch-Clamp Techniques
Plasmids/genetics
Receptors
Signal Transduction/drug effects
Superior Cervical Ganglion/cytology/drug effects/metabolism
Sympathetic Nervous System/cytology/drug effects/metabolism
The Journal of pharmacology and experimental therapeutics
Western
Yun June
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1124/dmd.105.007575" target="_blank" rel="noreferrer noopener">http://doi.org/10.1124/dmd.105.007575</a>
Pages
756–764
Issue
5
Volume
34
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Rifampicin induction of CYP3A4 requires pregnane X receptor cross talk with hepatocyte nuclear factor 4alpha and coactivators, and suppression of small heterodimer partner gene expression.
Publisher
An entity responsible for making the resource available
Drug metabolism and disposition: the biological fate of chemicals
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-05
Subject
The topic of the resource
Antibiotics; Antitubercular/*pharmacology; Blotting; Cell Line; Chromatin/metabolism; Cytochrome P-450 CYP3A; Cytochrome P-450 Enzyme System/*biosynthesis; Cytoplasmic and Nuclear/*biosynthesis/*drug effects/genetics; Electrophoretic Mobility Shift Assay; Enzyme Induction/drug effects; Glutathione Transferase/metabolism; Hepatocyte Nuclear Factor 4/genetics/*metabolism; Hepatocytes/drug effects/metabolism; Humans; Immunoprecipitation; Messenger/biosynthesis; Plasmids/genetics; Pregnane X Receptor; Receptor Cross-Talk/drug effects; Receptors; Reverse Transcriptase Polymerase Chain Reaction; Rifampin/*pharmacology; RNA; Steroid/*drug effects/genetics; Transfection; Tumor; Western
Creator
An entity primarily responsible for making the resource
Li Tiangang; Chiang John Y L
Description
An account of the resource
Bile acids and drugs activate pregnane X receptor (PXR) to induce CYP3A4, which is the predominant cytochrome P450 enzyme expressed in the liver and intestine and plays a critical role in detoxifying bile acids and drugs, and protecting against cholestasis. The aim of this study is to investigate the molecular mechanism of PXR cross talk with other nuclear receptors and coactivators in regulating human CYP3A4 gene transcription. Rifampicin dose dependently induced the CYP3A4 but inhibited small heterodimer partner (SHP) mRNA expression levels in primary human hepatocytes. Rifampicin strongly stimulated PXR and hepatocyte nuclear factor 4alpha (HNF4alpha) interaction, and CYP3A4 reporter activity, which was further stimulated by peroxisome proliferators-activated receptorgamma co-activator 1alpha (PGC-1alpha) and steroid receptor coactivator-1 (SRC-1) but inhibited by SHP. Mutation of the putative HNF4alpha binding site in the distal xenobiotic responsive element module did not affect CYP3A4 basal promoter activity and synergistic stimulation by PXR and HNF4alpha. Chromatin immunoprecipitation assays revealed that rifampicin-activated PXR recruited HNF4alpha and SRC-1 to the CYP3A4 chromatin. On the other hand, SHP reduced PXR recruitment of HNF4alpha and SRC-1 to the CYP3A4 chromatin. The human SHP promoter was stimulated by HNF4alpha and PGC-1alpha. Upon activation by rifampicin, PXR inhibited SHP promoter activity. Results suggest that PXR strongly induces CYP3A4 gene transcription by interacting with HNF4alpha, SRC-1, and PGC-1alpha. PXR concomitantly inhibits SHP gene transcription and maximizes the PXR induction of the CYP3A4 gene in human livers. Drugs targeted to PXR may be developed for treating cholestatic liver diseases induced by bile acids and drugs.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1124/dmd.105.007575" target="_blank" rel="noreferrer noopener">10.1124/dmd.105.007575</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2006
Antibiotics
Antitubercular/*pharmacology
Blotting
Cell Line
Chiang John Y L
Chromatin/metabolism
Cytochrome P-450 CYP3A
Cytochrome P-450 Enzyme System/*biosynthesis
Cytoplasmic and Nuclear/*biosynthesis/*drug effects/genetics
Department of Integrative Medical Sciences
Drug metabolism and disposition: the biological fate of chemicals
Electrophoretic Mobility Shift Assay
Enzyme Induction/drug effects
Glutathione Transferase/metabolism
Hepatocyte Nuclear Factor 4/genetics/*metabolism
Hepatocytes/drug effects/metabolism
Humans
Immunoprecipitation
Li Tiangang
Messenger/biosynthesis
NEOMED College of Medicine
Plasmids/genetics
Pregnane X Receptor
Receptor Cross-Talk/drug effects
Receptors
Reverse Transcriptase Polymerase Chain Reaction
Rifampin/*pharmacology
RNA
Steroid/*drug effects/genetics
Transfection
Tumor
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1093/carcin/bgr045" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/carcin/bgr045</a>
Pages
888–896
Issue
6
Volume
32
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Pomegranate-mediated chemoprevention of experimental hepatocarcinogenesis involves Nrf2-regulated antioxidant mechanisms.
Publisher
An entity responsible for making the resource available
Carcinogenesis
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-06
Subject
The topic of the resource
*Phytotherapy; Alkylating Agents/toxicity; Animals; Antioxidants/*therapeutic use; Blotting; Carcinoma; Diethylnitrosamine/toxicity; Experimental/chemically induced/*drug therapy/metabolism; GA-Binding Protein Transcription Factor/*metabolism; gamma-Glutamyltransferase/metabolism; Hepatocellular/chemically induced/*drug therapy/metabolism; Immunoenzyme Techniques; Lipid Peroxidation/drug effects; Liver Neoplasms; Male; Oxidative Stress; Plant Extracts/*therapeutic use; Punicaceae/*chemistry; Rats; Sprague-Dawley; Western
Creator
An entity primarily responsible for making the resource
Bishayee Anupam; Bhatia Deepak; Thoppil Roslin J; Darvesh Altaf S; Nevo Eviatar; Lansky Ephraim P
Description
An account of the resource
Hepatocellular carcinoma (HCC), one of the most prevalent and lethal cancers, has shown an alarming rise in the USA. Without effective therapy for HCC, novel chemopreventive strategies may effectively circumvent the current morbidity and mortality. Oxidative stress predisposes to hepatocarcinogenesis and is the major driving force of HCC. Pomegranate, an ancient fruit, is gaining tremendous attention due to its powerful antioxidant properties. Here, we examined mechanism-based chemopreventive potential of a pomegranate emulsion (PE) against dietary carcinogen diethylnitrosamine (DENA)-induced rat hepatocarcinogenesis that mimics human HCC. PE treatment (1 or 10 g/kg), started 4 weeks prior to the DENA challenge and continued for 18 weeks thereafter, showed striking chemopreventive activity demonstrated by reduced incidence, number, multiplicity, size and volume of hepatic nodules, precursors of HCC. Both doses of PE significantly attenuated the number and area of gamma-glutamyl transpeptidase-positive hepatic foci compared with the DENA control. PE also attenuated DENA-induced hepatic lipid peroxidation and protein oxidation. Mechanistic studies revealed that PE elevated gene expression of an array of hepatic antioxidant and carcinogen detoxifying enzymes in DENA-exposed animals. PE elevated protein and messenger RNA expression of the hepatic nuclear factor
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1093/carcin/bgr045" target="_blank" rel="noreferrer noopener">10.1093/carcin/bgr045</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Phytotherapy
2011
Alkylating Agents/toxicity
Animals
Antioxidants/*therapeutic use
Bhatia Deepak
Bishayee Anupam
Blotting
Carcinogenesis
Carcinoma
Darvesh Altaf S
Department of Pharmaceutical Sciences
Diethylnitrosamine/toxicity
Experimental/chemically induced/*drug therapy/metabolism
GA-Binding Protein Transcription Factor/*metabolism
gamma-Glutamyltransferase/metabolism
Hepatocellular/chemically induced/*drug therapy/metabolism
Immunoenzyme Techniques
Lansky Ephraim P
Lipid Peroxidation/drug effects
Liver Neoplasms
Male
NEOMED College of Pharmacy
Nevo Eviatar
Oxidative Stress
Plant Extracts/*therapeutic use
Punicaceae/*chemistry
Rats
Sprague-Dawley
Thoppil Roslin J
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1089/neu.2015.3970" target="_blank" rel="noreferrer noopener">http://doi.org/10.1089/neu.2015.3970</a>
Pages
625–640
Issue
7
Volume
33
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Altered Neuroinflammation and Behavior after Traumatic Brain Injury in a Mouse Model of Alzheimer's Disease.
Publisher
An entity responsible for making the resource available
Journal of neurotrauma
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-04
Subject
The topic of the resource
Alzheimer Disease/etiology/*metabolism/pathology; Alzheimer's disease; Amyloid beta-Peptides/*metabolism; Animal; Animal/physiology; Animals; Behavior; Blotting; Brain Injuries; Brain/*metabolism/pathology; Disease Models; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Humans; Immunohistochemistry; Inbred C57BL; Inflammation/*metabolism/pathology; macrophage; Mice; neuroinflammation; Transgenic; traumatic brain injury; Traumatic/complications/*metabolism/*pathology; Western
Creator
An entity primarily responsible for making the resource
Kokiko-Cochran Olga N; Ransohoff Lena; Veenstra Mike; Lee Sungho; Saber Maha; Sikora Matt; Teknipp Ryan; Xu Guixiang; Bemiller Shane M; Wilson Gina; Crish Samuel; Bhaskar Kiran; Lee Yu-Shang; Ransohoff Richard M; Lamb Bruce T
Description
An account of the resource
Traumatic brain injury (TBI) has acute and chronic sequelae, including an increased risk for the development of Alzheimer's disease (AD). TBI-associated neuroinflammation is characterized by activation of brain-resident microglia and infiltration of monocytes; however, recent studies have implicated beta-amyloid as a major manipulator of the inflammatory response. To examine neuroinflammation after TBI and development of AD-like features, these studies examined the effects of TBI in the presence and absence of beta-amyloid. The R1.40 mouse model of cerebral amyloidosis was used, with a focus on time points well before robust AD pathologies. Unexpectedly, in R1.40 mice, the acute neuroinflammatory response to TBI was strikingly muted, with reduced numbers of CNS myeloid cells acquiring a macrophage phenotype and decreased expression of inflammatory cytokines. At chronic time points, macrophage activation substantially declined in non-Tg TBI mice; however, it was relatively unchanged in R1.40 TBI mice. The persistent inflammatory response coincided with significant tissue loss between 3 and 120 days post-injury in R1.40 TBI mice, which was not observed in non-Tg TBI mice. Surprisingly, inflammatory cytokine expression was enhanced in R1.40 mice compared with non-Tg mice, regardless of injury group. Although R1.40 TBI mice demonstrated task-specific deficits in cognition, overall functional recovery was similar to non-Tg TBI mice. These findings suggest that accumulating beta-amyloid leads to an altered post-injury macrophage response at acute and chronic time points. Together, these studies emphasize the role of post-injury neuroinflammation in regulating long-term sequelae after TBI and also support recent studies implicating beta-amyloid as an immunomodulator.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1089/neu.2015.3970" target="_blank" rel="noreferrer noopener">10.1089/neu.2015.3970</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2016
Alzheimer Disease/etiology/*metabolism/pathology
Alzheimer's disease
Amyloid beta-Peptides/*metabolism
Animal
Animal/physiology
Animals
Behavior
Bemiller Shane M
Bhaskar Kiran
Blotting
Brain Injuries
Brain/*metabolism/pathology
Crish Samuel
Department of Pharmaceutical Sciences
Disease Models
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Humans
Immunohistochemistry
Inbred C57BL
Inflammation/*metabolism/pathology
Journal of neurotrauma
Kokiko-Cochran Olga N
Lamb Bruce T
Lee Sungho
Lee Yu-Shang
macrophage
Mice
NEOMED College of Pharmacy
Neuroinflammation
Ransohoff Lena
Ransohoff Richard M
Saber Maha
Sikora Matt
Teknipp Ryan
Transgenic
Traumatic brain injury
Traumatic/complications/*metabolism/*pathology
Veenstra Mike
Western
Wilson Gina
Xu Guixiang
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M502751200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M502751200</a>
Pages
30517–30525
Issue
34
Volume
280
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bcl-2 positively regulates Sox9-dependent chondrocyte gene expression by suppressing the MEK-ERK1/2 signaling pathway.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-08
Subject
The topic of the resource
*Gene Expression Regulation; Adenoviridae/genetics; Animals; Apoptosis; beta-Galactosidase/metabolism; Blotting; Butadienes/pharmacology; Caspase Inhibitors; Cell Differentiation; Cell Line; Chondrocytes/*metabolism; Collagen Type II/metabolism; Down-Regulation; Enzyme Inhibitors/pharmacology; Fibroblasts/metabolism; Fluorescence; Genetic; High Mobility Group Proteins/*metabolism; Lac Operon; Luciferases/metabolism; MAP Kinase Kinase Kinases/*metabolism; Messenger/metabolism; Microscopy; Mitogen-Activated Protein Kinase 1/*metabolism; Mitogen-Activated Protein Kinase 3/*metabolism; NF-kappa B/metabolism; Nitriles/pharmacology; Phenotype; Phosphorylation; Promoter Regions; Protein Kinase C-alpha; Protein Kinase C/antagonists & inhibitors; Proteoglycans/metabolism; Proto-Oncogene Proteins c-bcl-2/*metabolism; Rats; Reverse Transcriptase Polymerase Chain Reaction; RNA; Signal Transduction; Small Interfering/metabolism; SOX9 Transcription Factor; Sprague-Dawley; Time Factors; Transcription; Transcription Factors/*metabolism; Transfection; Western
Creator
An entity primarily responsible for making the resource
Yagi Rieko; McBurney Denise; Horton Walter E Jr
Description
An account of the resource
Bcl-2 is an anti-apoptotic protein that has recently been shown to regulate other cellular functions. We previously reported that Bcl-2 regulates chondrocyte matrix gene expression, independent of its anti-apoptotic function. Here, we further investigate this novel function of Bcl-2 and examine three intracellular signaling pathways likely to be associated with this function. The present study demonstrates that the activity of Sox9, a master transcription factor that regulates the gene expression of chondrocyte matrix proteins, is suppressed by Bcl-2 small interference RNA in the presence of caspase inhibitors. This effect was attenuated by prior exposure of chondrocytes to an adenoviral vector expressing sense Bcl-2. In addition, the down-regulation of Bcl-2, Sox9, and chondrocyte-specific gene expression by serum withdrawal in primary chondrocytes was reversed by expressing Bcl-2. Inhibition of the protein kinase C alpha and NFkappaB pathways had no effect on the maintenance of Sox9-dependent gene expression by Bcl-2. In contrast, whereas the MEK-ERK1/2 pathway negatively regulated the differentiated phenotype in wild type chondrocytes, inhibition of this pathway reversed the loss of differentiation markers and fibroblastic phenotype in Bcl-2-deficient chondrocytes. In conclusion, the present study identifies a specific signaling pathway, namely, MEK-ERK1/2, that is downstream of Bcl-2 in the regulation of Sox9-dependent chondrocyte gene expression and phenotype.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M502751200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M502751200</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
2005
Adenoviridae/genetics
Animals
Apoptosis
beta-Galactosidase/metabolism
Blotting
Butadienes/pharmacology
Caspase Inhibitors
Cell Differentiation
Cell Line
Chondrocytes/*metabolism
Collagen Type II/metabolism
Department of Anatomy & Neurobiology
Down-Regulation
Enzyme Inhibitors/pharmacology
Fibroblasts/metabolism
Fluorescence
Genetic
High Mobility Group Proteins/*metabolism
Horton Walter E Jr
Lac Operon
Luciferases/metabolism
MAP Kinase Kinase Kinases/*metabolism
McBurney Denise
Messenger/metabolism
Microscopy
Mitogen-Activated Protein Kinase 1/*metabolism
Mitogen-Activated Protein Kinase 3/*metabolism
NEOMED College of Medicine
NF-kappa B/metabolism
Nitriles/pharmacology
Phenotype
Phosphorylation
Promoter Regions
Protein Kinase C-alpha
Protein Kinase C/antagonists & inhibitors
Proteoglycans/metabolism
Proto-Oncogene Proteins c-bcl-2/*metabolism
Rats
Reverse Transcriptase Polymerase Chain Reaction
RNA
Signal Transduction
Small Interfering/metabolism
SOX9 Transcription Factor
Sprague-Dawley
The Journal of biological chemistry
Time Factors
Transcription
Transcription Factors/*metabolism
Transfection
Western
Yagi Rieko
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/S0003-9861(02)00066-8" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/S0003-9861(02)00066-8</a>
Pages
84–93
Issue
1
Volume
402
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Phosphatidylinositide 3-kinase regulates angiotensin II-induced cytosolic phospholipase A2 activity and growth in vascular smooth muscle cells.
Publisher
An entity responsible for making the resource available
Archives of biochemistry and biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-06
Subject
The topic of the resource
Angiotensin II/*metabolism; Animals; Arachidonic Acid/metabolism; Arachidonic Acids/pharmacology; Blotting; Cells; Chromones/pharmacology; Cultured; Enzyme Inhibitors/pharmacology; Flavonoids/pharmacology; Group IV Phospholipases A2; Male; Mitogen-Activated Protein Kinases/metabolism; Morpholines/pharmacology; Muscle; Phosphatidylinositol 3-Kinases/*physiology; Phospholipases A/*metabolism; Phospholipases A2; Phosphorylation; Rats; Smooth; Sprague-Dawley; Vascular/drug effects/*growth & development; Western
Creator
An entity primarily responsible for making the resource
Silfani Tonous N; Freeman Ernest J
Description
An account of the resource
Angiotensin (Ang) II via the AT(1) receptor acts as a mitogen in vascular smooth muscle cells (VSMC) through stimulation of multiple signaling mechanisms, including tyrosine kinases and mitogen-activated protein kinase (MAPK). In addition, cytosolic phospholipase A(2)(cPLA(2))-dependent release of arachidonic acid (AA) is linked to VSMC growth and we have reported that Ang II stimulates cPLA(2) activity via the AT(1) receptor. The coupling of Ang II to the activation of cPLA(2) appears to involve mechanisms both upstream and downstream of MAPK such that AA stimulates MAPK activity which phosphorylates cPLA(2) to further enhance AA release. However, the upstream mechanisms responsible for activation of cPLA(2) are not well-defined. One possibility includes phosphatidylinositide
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/S0003-9861(02)00066-8" target="_blank" rel="noreferrer noopener">10.1016/S0003-9861(02)00066-8</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2002
Angiotensin II/*metabolism
Animals
Arachidonic Acid/metabolism
Arachidonic Acids/pharmacology
Archives of biochemistry and biophysics
Blotting
Cells
Chromones/pharmacology
Cultured
Enzyme Inhibitors/pharmacology
Flavonoids/pharmacology
Freeman Ernest J
Group IV Phospholipases A2
Male
Mitogen-Activated Protein Kinases/metabolism
Morpholines/pharmacology
Muscle
Phosphatidylinositol 3-Kinases/*physiology
Phospholipases A/*metabolism
Phospholipases A2
Phosphorylation
Rats
Silfani Tonous N
Smooth
Sprague-Dawley
Vascular/drug effects/*growth & development
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.ntt.2012.03.003" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.ntt.2012.03.003</a>
Pages
338–343
Issue
3
Volume
34
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Markers associated with testosterone enhancement of methamphetamine-induced striatal dopaminergic neurotoxicity.
Publisher
An entity responsible for making the resource available
Neurotoxicology and teratology
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-06
Subject
The topic of the resource
Animals; Biomarkers/metabolism; Blotting; Body Temperature/drug effects; Body Weight/drug effects; Corpus Striatum/*drug effects/metabolism; Dopamine Plasma Membrane Transport Proteins/metabolism; Dopamine/*metabolism; Drug Synergism; HSP70 Heat-Shock Proteins/metabolism; Inbred Strains; Male; Methamphetamine/*toxicity; Mice; Neurotoxicity Syndromes/*etiology/metabolism; Oxidative Stress/drug effects; Testosterone Propionate/*pharmacology; Vesicular Monoamine Transport Proteins/metabolism; Western
Creator
An entity primarily responsible for making the resource
Buletko A Blake; Dluzen Dean E; McDermott Janet L; Darvesh Altaf S; Geldenhuys Werner J
Description
An account of the resource
Intact male CD-1 mice received an injection of testosterone propionate (TP–5 ug), progesterone (P–5 mg), the oil vehicle or remained untreated (control). At 24 hours after hormonal treatments the mice received an injection of methamphetamine (MA–40 mg/kg) and rectal temperatures were measured. At 5 days post-MA, assays were performed to assess effects of these treatments. Maximal increases in body temperatures, that were significantly greater than oil-treated controls, were obtained in TP-treated mice. At 5 days post-MA, maximal weight reductions were obtained with TP-treated mice, while P-treated mice showed no significant decrease between the pre- versus post-MA determinations. Striatal dopamine concentrations showed maximal reductions and heat-shock protein-70 maximal increases in the TP group, with both differing significantly as compared with all other groups. Protein levels of dopamine transporters were significantly decreased in P-treated mice, while vesicular monoamine transporter-2 was significantly decreased in TP-treated mice. Taken together, these results suggest that testosterone exacerbates the deleterious effects of MA within male mice as indicated by a number of markers related to neurotoxicity. The changes in markers as associated with this enhanced neurotoxicity suggest that TP may increase thermal/energy responses and/or oxidative stress to produce this effect.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.ntt.2012.03.003" target="_blank" rel="noreferrer noopener">10.1016/j.ntt.2012.03.003</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2012
Animals
Biomarkers/metabolism
Blotting
Body Temperature/drug effects
Body Weight/drug effects
Buletko A Blake
Corpus Striatum/*drug effects/metabolism
Darvesh Altaf S
Department of Pharmaceutical Sciences
Dluzen Dean E
Dopamine Plasma Membrane Transport Proteins/metabolism
Dopamine/*metabolism
Drug Synergism
Geldenhuys Werner J
HSP70 Heat-Shock Proteins/metabolism
Inbred Strains
Male
McDermott Janet L
Methamphetamine/*toxicity
Mice
NEOMED College of Pharmacy
Neurotoxicity Syndromes/*etiology/metabolism
Neurotoxicology and teratology
Oxidative Stress/drug effects
Testosterone Propionate/*pharmacology
Vesicular Monoamine Transport Proteins/metabolism
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.antiviral.2006.07.004" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.antiviral.2006.07.004</a>
Pages
171–177
Issue
3
Volume
72
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Resveratrol inhibition of varicella-zoster virus replication in vitro.
Publisher
An entity responsible for making the resource available
Antiviral research
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-12
Subject
The topic of the resource
Antiviral Agents/*pharmacology; Blotting; Cell Line; Dose-Response Relationship; Drug; Fibroblasts/virology; Herpesvirus 3; Human/*drug effects/physiology; Humans; Immediate-Early Proteins/biosynthesis; Messenger/biosynthesis; Resveratrol; Reverse Transcriptase Polymerase Chain Reaction; RNA; Stilbenes/*pharmacology; Trans-Activators/biosynthesis; Viral Envelope Proteins/biosynthesis; Viral Plaque Assay; Viral/biosynthesis; Virus Attachment/drug effects; Virus Inactivation/drug effects; Virus Replication/*drug effects; Western
Creator
An entity primarily responsible for making the resource
Docherty John J; Sweet Thomas J; Bailey Erin; Faith Seth A; Booth Tristan
Description
An account of the resource
Resveratrol was found to inhibit varicella-zoster virus (VZV) replication in a dose-dependent and reversible manner. This decrease in virus production in the presence of resveratrol was not caused by direct inactivation of VZV or inhibition of virus attachment to MRC-5 cells. The drug effectively limited VZV replication if added during the first 30 h of infection. Western blot analysis and real-time RT-PCR studies demonstrated that protein and mRNA levels of IE62, an essential immediate early viral protein, were reduced when compared to controls. These results demonstrate that VZV replication is adversely affected by resveratrol which is negatively impacting IE62 synthesis.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.antiviral.2006.07.004" target="_blank" rel="noreferrer noopener">10.1016/j.antiviral.2006.07.004</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2006
Antiviral Agents/*pharmacology
Antiviral research
Bailey Erin
Blotting
Booth Tristan
Cell Line
Docherty John J
Dose-Response Relationship
Drug
Faith Seth A
Fibroblasts/virology
Herpesvirus 3
Human/*drug effects/physiology
Humans
Immediate-Early Proteins/biosynthesis
Messenger/biosynthesis
Resveratrol
Reverse Transcriptase Polymerase Chain Reaction
RNA
Stilbenes/*pharmacology
Sweet Thomas J
Trans-Activators/biosynthesis
Viral Envelope Proteins/biosynthesis
Viral Plaque Assay
Viral/biosynthesis
Virus Attachment/drug effects
Virus Inactivation/drug effects
Virus Replication/*drug effects
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.ajpath.2016.06.010" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.ajpath.2016.06.010</a>
Pages
2701–2708
Issue
10
Volume
186
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Histone Deacetylase Inhibitor Vorinostat (SAHA) Suppresses IL-1beta-Induced Matrix Metallopeptidase-13 Expression by Inhibiting IL-6 in Osteoarthritis Chondrocyte.
Publisher
An entity responsible for making the resource available
The American journal of pathology
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-10
Subject
The topic of the resource
Aged; Articular/drug effects/metabolism; Blotting; Cartilage; Chondrocytes/drug effects; Collagen Type II/drug effects/metabolism; Down-Regulation/drug effects; Female; Gene Expression Regulation/*drug effects; Histone Deacetylase Inhibitors/*pharmacology; Humans; Hydroxamic Acids/*pharmacology; Interleukin-1beta/*antagonists & inhibitors/genetics/metabolism; Knee Joint/metabolism; Male; Matrix Metalloproteinase 13/*drug effects/metabolism; Middle Aged; Osteoarthritis/*drug therapy; Proteoglycans/metabolism; Tumor Necrosis Factor-alpha/drug effects/metabolism; Vorinostat; Western
Creator
An entity primarily responsible for making the resource
Makki Mohammad Shahidul; Haqqi Tariq M
Description
An account of the resource
Osteoarthritis (OA) is the most common whole-joint disease and is characterized by progressive loss of the cartilage matrix. Matrix metallopeptidase-13 (MMP-13) is a highly active and an abundantly expressed protease in OA cartilage and chondrocytes and degrades type II collagen and proteoglycans. We investigated the mechanism of MMP-13 suppression by histone deacetylase inhibitor vorinostat (SAHA). OA chondrocytes were obtained from knee cartilage after enzymatic digestion and treated with IL-1beta in the absence or presence of various histone deacetylase inhibitors. Gene expression was quantified using quantitative RT-PCR. Protein expression and chromatin modifications were determined by Western immunoblotting using specific antibodies. The effect of IL-6 on the expression of
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.ajpath.2016.06.010" target="_blank" rel="noreferrer noopener">10.1016/j.ajpath.2016.06.010</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2016
Aged
Articular/drug effects/metabolism
Blotting
Cartilage
Chondrocytes/drug effects
Collagen Type II/drug effects/metabolism
Department of Anatomy & Neurobiology
Down-Regulation/drug effects
Female
Gene Expression Regulation/*drug effects
Haqqi Tariq M
Histone Deacetylase Inhibitors/*pharmacology
Humans
Hydroxamic Acids/*pharmacology
Interleukin-1beta/*antagonists & inhibitors/genetics/metabolism
Knee Joint/metabolism
Makki Mohammad Shahidul
Male
Matrix Metalloproteinase 13/*drug effects/metabolism
Middle Aged
NEOMED College of Medicine
Osteoarthritis/*drug therapy
Proteoglycans/metabolism
The American journal of pathology
Tumor Necrosis Factor-alpha/drug effects/metabolism
Vorinostat
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.ajpath.2016.05.006" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.ajpath.2016.05.006</a>
Pages
2417–2428
Issue
9
Volume
186
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The Detrimental Role Played by Lipocalin-2 in Alcoholic Fatty Liver in Mice.
Publisher
An entity responsible for making the resource available
The American journal of pathology
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-09
Subject
The topic of the resource
Alcoholic/*metabolism; Animal; Animals; Blotting; Disease Models; Fatty Liver; Humans; Inbred C57BL; Knockout; Lipocalin-2/*metabolism; Mice; Polymerase Chain Reaction; Western
Creator
An entity primarily responsible for making the resource
Cai Yan; Jogasuria Alvin; Yin Huquan; Xu Ming-Jiang; Hu Xudong; Wang Jiayou; Kim Chunki; Wu Jiashin; Lee Kwangwon; Gao Bin; You Min
Description
An account of the resource
We have previously shown that the ethanol-mediated elevation of lipocaline-2 (LCN2) is closely associated with the development of alcoholic fatty liver disease (AFLD) in mice. Herein, we aimed to understand the functional significance of LCN2 induction by ethanol and to explore its underlying mechanisms. We evaluated the effects of LCN2 in an in vitro cellular alcoholic steatosis model and in an animal study using wild-type and LCN2 knockout mice fed for 4 weeks with an ethanol-supplemented Lieber-DeCarli diet. In the cellular model of alcoholic steatosis, recombinant LCN2 or overexpression of LCN2 exacerbated ethanol-induced fat accumulation, whereas knocking down LCN2 prevented steatosis in hepatocytes exposed to ethanol. Consistently, removal of LCN2 partially but significantly alleviated alcoholic fatty liver injury in mice. Mechanistically, LCN2 mediates detrimental effects of ethanol in the liver via disrupted multiple signaling pathways, including aberrant nicotinamide phosphoribosyltransferase-sirtuin 1 axis, perturbed endocrine metabolic regulatory fibroblast growth factor 15/19 signaling, and impaired chaperone-mediated autophagy. Finally, compared with healthy human livers, liver samples from patients with AFLD had lower gene expression of several
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.ajpath.2016.05.006" target="_blank" rel="noreferrer noopener">10.1016/j.ajpath.2016.05.006</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2016
Alcoholic/*metabolism
Animal
Animals
Blotting
Cai Yan
Department of Pharmaceutical Sciences
Disease Models
Fatty Liver
Gao Bin
Hu Xudong
Humans
Inbred C57BL
Jogasuria Alvin
Kim Chunki
Knockout
Lee Kwangwon
Lipocalin-2/*metabolism
Mice
NEOMED College of Pharmacy
Polymerase Chain Reaction
The American journal of pathology
Wang Jiayou
Western
Wu Jiashin
Xu Ming-Jiang
Yin Huquan
You Min
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s11655-016-2621-z" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s11655-016-2621-z</a>
Pages
40–47
Issue
1
Volume
23
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Salvianolate reduces murine myocardial ischemia and reperfusion injury via ERK1/2 signaling pathways in vivo.
Publisher
An entity responsible for making the resource available
Chinese journal of integrative medicine
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-01
Subject
The topic of the resource
*MAP Kinase Signaling System/drug effects; Animal Studies; Animals; Blotting; Cardiotonic Agents/pharmacology/therapeutic use; Chinese medicine; Descriptive Statistics; extracellular signal-regulated kinase 1/2; Flavonoids/pharmacology; Heart Ventricle; Heart Ventricles/drug effects/pathology; In Vivo Studies; Inbred C57BL; ischemia and reperfusion injury; Left; Male; Mice; Mitogen-Activated Protein Kinase 1/*metabolism; Mitogen-Activated Protein Kinase 3/*metabolism; Molecular Structure; Myocardial Ischemia – Drug Therapy; Myocardial Reperfusion Injury – Drug Therapy; Myocardial Reperfusion Injury/*drug therapy/enzymology/pathology; Organ Size/drug effects; P-Value; Phosphorylation; Phosphorylation/drug effects; Plant Extracts – Administration and Dosage; Plant Extracts/chemistry/pharmacology/*therapeutic use; protein kinase B; Protein Kinase Inhibitors – Administration and Dosage; Protein Kinase Inhibitors/pharmacology; Protein Kinases – Analysis; Protein Kinases – Drug Effects; salvianolate; Signal Transduction; Staining and Labeling; Western
Creator
An entity primarily responsible for making the resource
Qi Jianyong; Yu Juan; Huang Dong-Hui; Guo Liheng; Wang Lei; Huang Xin; Huang Hai-Ding; Zhou Miao; Zhang Minzhou; Wu Jiashin
Description
An account of the resource
OBJECTIVE: To analyze the effects of salvianolate on myocardial infarction in a murine in vivo model of ischemia and reperfusion (I/R) injury. METHODS: Myocardial I/R injury model was constructed in mice by 30 min of coronary occlusion followed by 24 h of reperfusion and pretreated with salvianolate 30 min before I/R (SAL group). The SAL group was compared with SHAM (no I/R and no salvianolate), I/R (no salvianolate), and ischemia preconditioning (IPC) groups. Furthermore, an ERK1/2 inhibitor PD98059 (1 mg/kg), and a phosphatidylinositol-3-kinase (PI3-K) inhibitor, LY294002 (7.5 mg/kg), were administered intraperitoneal injection (i.p) for 30 min prior to salvianolate, followed by I/R surgery in LY and PD groups. By using a double staining method, the ratio of the infarct size (IS) to left ventricle (LV) and of risk region (RR) to LV were compared among the groups. Correlations between IS and RR were analyzed. Western-blot was used to detect the extracellular signal-regulated kinase 1/2 (ERK1/2) and protein kinase B (AKT) phosphorylation changes. RESULTS: There were no significant differences between RR to LV ratio among the SHAM, I/R, IPC and SAL groups (P\textgreater0.05). The SAL and IPC groups had IS of 26.1%+/-1.4% and 22.3%+/-2.9% of RR, respectively, both of which were significantly smaller than the I/R group (38.5%+/-2.9% of RR, P\textless0.05, P\textless0.01, respectively). Moreover, the phosphorylation of ERK1/2 was increased in SAL group (P\textless0.05), while AKT had no significant change. LY294002 further reduced IS, whereas the protective role of salvianolate could be attenuated by PD98059, which increased the IS. Additionally, the IS was not linearly related to the RR (r=0.23, 0.45, 0.62, 0.17, and 0.52 in the SHAM, I/R, SAL, LY and PD groups, respectively). CONCLUSION: Salvianolate could reduce myocardial I/R injury in mice in vivo, which involves an ERK1/2 pathway, but not a PI3-K signaling pathway.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/s11655-016-2621-z" target="_blank" rel="noreferrer noopener">10.1007/s11655-016-2621-z</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*MAP Kinase Signaling System/drug effects
2017
Animal Studies
Animals
Blotting
Cardiotonic Agents/pharmacology/therapeutic use
Chinese journal of integrative medicine
Chinese medicine
Descriptive Statistics
extracellular signal-regulated kinase 1/2
Flavonoids/pharmacology
Guo Liheng
Heart Ventricle
Heart Ventricles/drug effects/pathology
Huang Dong-Hui
Huang Hai-Ding
Huang Xin
In Vivo Studies
Inbred C57BL
ischemia and reperfusion injury
Left
Male
Mice
Mitogen-Activated Protein Kinase 1/*metabolism
Mitogen-Activated Protein Kinase 3/*metabolism
Molecular Structure
Myocardial Ischemia – Drug Therapy
Myocardial Reperfusion Injury – Drug Therapy
Myocardial Reperfusion Injury/*drug therapy/enzymology/pathology
Organ Size/drug effects
P-Value
Phosphorylation
Phosphorylation/drug effects
Plant Extracts – Administration and Dosage
Plant Extracts/chemistry/pharmacology/*therapeutic use
protein kinase B
Protein Kinase Inhibitors – Administration and Dosage
Protein Kinase Inhibitors/pharmacology
Protein Kinases – Analysis
Protein Kinases – Drug Effects
Qi Jianyong
salvianolate
Signal Transduction
Staining and Labeling
Wang Lei
Western
Wu Jiashin
Yu Juan
Zhang Minzhou
Zhou Miao
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s10637-009-9332-7" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s10637-009-9332-7</a>
Pages
380–391
Issue
2
Volume
29
Dublin Core
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Title
A name given to the resource
Chemopreventive doses of resveratrol do not produce cardiotoxicity in a rodent model of hepatocellular carcinoma.
Publisher
An entity responsible for making the resource available
Investigational new drugs
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-04
Subject
The topic of the resource
*Chemoprevention; Analysis of Variance; Animal; Animal Studies; Animal/drug effects; Animals; Antioxidants; Behavior; Blotting; Carcinoma; Cardiotoxicity; Cardiotoxins/*toxicity; Chemoprevention; Data Analysis Software; Descriptive Statistics; Disease Models; Doppler; Dose-Response Relationship; Drug; Echocardiography; Feeding Behavior/drug effects; Female; Fisher's Exact Test; Funding Source; Heart – Drug Effects; Heart/drug effects/physiopathology; Hepatocellular – Prevention and Control; Hepatocellular/*drug therapy/pathology/physiopathology; Hepatocytes/drug effects/pathology; Humans; Liver Neoplasms/*drug therapy/pathology/physiopathology; Liver/drug effects/pathology/physiopathology; Polyphenols – Therapeutic Use; Rats; Resveratrol; Sprague-Dawley; Stilbenes/*therapeutic use; Systole/drug effects; Western
Creator
An entity primarily responsible for making the resource
Luther Daniel J; Ohanyan Vahagn; Shamhart Patricia E; Hodnichak Cheryl M; Sisakian Hamayak; Booth Tristan D; Meszaros J Gary; Bishayee Anupam
Description
An account of the resource
Hepatocellular carcinoma (HCC), one of the most lethal cancers, results in more than one million fatalities worldwide every year. In view of the limited therapeutic alternatives and poor prognosis of liver cancer, preventive control approaches, notably chemoprevention, have been considered to be the best strategy in lowering the present prevalence of the disease. Resveratrol, a naturally occurring antioxidant and antiinflammatory agent found in grapes and red wine, inhibits carcinogenesis with a pleiotropic mode of action. Recently, we have reported that dietary resveratrol significantly prevents chemically-induced liver tumorigenesis in rats. One of the mechanisms of resveratrol-mediated chemoprevention of hepatocarcinogenesis could be related to its antiinflammatory action through hepatic cyclooxygenase (COX-2) inhibition. Although several COX-2 inhibitors are known to exert chemopreventive efficacy, not all are considered ideal candidates for chemoprevention due to the risk of adverse cardiovascular events. Accordingly, the objective of the present study was to evaluate the role of resveratrol on cardiac performance during experimental hepatocarcinogenesis initiated with diethylnitrosamine and promoted by phenobarbital. Rats had free access to diet supplemented with resveratrol four weeks before the carcinogen injection and 14 weeks thereafter. The cardiotoxicity of resveratrol was assessed by monitoring the cardiac function using transthoracic echocardiography as well as Western blot analysis of cardiac tissue. Long-term dietary administration of resveratrol dose-dependently suppressed hepatic tumor multiplicity, the principal endpoint for evaluating the chemopreventive potential of a candidate agent. The chemopreventive effects of resveratrol were also reflected in histopathological assessment of hepatic tissues. Resveratrol did not exhibit any cardiotoxicity but rather improved the cardiac function in a dose-responsive fashion. Our results indicate that resveratrol-mediated chemoprevention of rat liver carcinogenesis is devoid of any adverse cardiovascular events. Resveratrol may be developed as a chemopreventive as well as therapeutic drug for human HCC.
Identifier
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<a href="http://doi.org/10.1007/s10637-009-9332-7" target="_blank" rel="noreferrer noopener">10.1007/s10637-009-9332-7</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Chemoprevention
2011
Analysis of Variance
Animal
Animal Studies
Animal/drug effects
Animals
Antioxidants
Behavior
Bishayee Anupam
Blotting
Booth Tristan D
Carcinoma
Cardiotoxicity
Cardiotoxins/*toxicity
Chemoprevention
Data Analysis Software
Department of Integrative Medical Sciences
Descriptive Statistics
Disease Models
Doppler
Dose-Response Relationship
Drug
Echocardiography
Feeding Behavior/drug effects
Female
Fisher's Exact Test
Funding Source
Heart – Drug Effects
Heart/drug effects/physiopathology
Hepatocellular – Prevention and Control
Hepatocellular/*drug therapy/pathology/physiopathology
Hepatocytes/drug effects/pathology
Hodnichak Cheryl M
Humans
Investigational new drugs
Liver Neoplasms/*drug therapy/pathology/physiopathology
Liver/drug effects/pathology/physiopathology
Luther Daniel J
Meszaros J Gary
NEOMED College of Medicine
Ohanyan Vahagn
Polyphenols – Therapeutic Use
Rats
Resveratrol
Shamhart Patricia E
Sisakian Hamayak
Sprague-Dawley
Stilbenes/*therapeutic use
Systole/drug effects
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s00441-005-1114-8" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s00441-005-1114-8</a>
Pages
35–44
Issue
1
Volume
321
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
P2X receptors in the rat uterine cervix, lumbosacral dorsal root ganglia, and spinal cord during pregnancy.
Publisher
An entity responsible for making the resource available
Cell and tissue research
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-07
Subject
The topic of the resource
Animals; Blotting; Cervix Uteri/*metabolism; Estrogen Receptor alpha/metabolism; Female; Frozen Sections; Ganglia; Immunohistochemistry; Lumbosacral Region; Pregnancy; Purinergic P2/*metabolism; Purinergic P2X; Rats; Receptors; Spinal Cord/cytology/*metabolism; Spinal/cytology/*metabolism; Sprague-Dawley; Western
Creator
An entity primarily responsible for making the resource
Papka Raymond E; Hafemeister Jen; Storey-Workley Megan
Description
An account of the resource
ATP, an intracellular energy source, is released from cells during tissue stress, damage, or inflammation. The P2X subtype of the ATP receptor is expressed in rat dorsal root ganglion (DRG) cells, spinal cord dorsal horn, and axons in peripheral tissues. ATP binding to P2X receptors on nociceptors generates signals that can be interpreted as pain from damaged tissue. We have hypothesized that tissue stress or damage in the uterine cervix during late pregnancy and parturition can lead to ATP release and sensory signaling via P2X receptors. Consequently, we have examined sensory pathways from the cervix in nonpregnant and pregnant rats for the presence of purinoceptors. Antiserum against the
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/s00441-005-1114-8" target="_blank" rel="noreferrer noopener">10.1007/s00441-005-1114-8</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2005
Animals
Blotting
Cell and tissue research
Cervix Uteri/*metabolism
Estrogen Receptor alpha/metabolism
Female
Frozen Sections
Ganglia
Hafemeister Jen
Immunohistochemistry
Lumbosacral Region
Papka Raymond E
Pregnancy
Purinergic P2/*metabolism
Purinergic P2X
Rats
Receptors
Spinal Cord/cytology/*metabolism
Spinal/cytology/*metabolism
Sprague-Dawley
Storey-Workley Megan
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/abbi.1993.1003" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/abbi.1993.1003</a>
Pages
18–23
Issue
1
Volume
300
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identification of a new P450 subfamily, CYP4F1, expressed in rat hepatic tumors.
Publisher
An entity responsible for making the resource available
Archives of biochemistry and biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-01
Subject
The topic of the resource
2-Acetylaminofluorene; Aflatoxin B1; Amino Acid; Amino Acid Sequence; Animals; Antisense Elements (Genetics); Base Sequence; Blotting; Clofibrate; Cytochrome P-450 Enzyme System/analysis/*genetics; Humans; Liver Neoplasms/chemically induced/*enzymology/genetics; Male; Microsomes/enzymology; Molecular Sequence Data; Multigene Family; Northern; Rats; Sequence Homology; Western
Creator
An entity primarily responsible for making the resource
Chen L; Hardwick J P
Description
An account of the resource
The expression of the rat cytochrome P450 CYP4 family was studied in hepatic tumors. In most of the primary and transplantable hepatic tumors studied, lauric acid omega-hydroxylase activity associated with the CYP4A subfamily enzymes decreased. The expression of CYP4A proteins and mRNAs in these tumors as assessed by Western and Northern blot was undetectable. However, while RNA analysis revealed the absence of 4A1, 4A2, and 4A3 mRNAs, the expression of CYP4 gene(s) was detected. A Uni-ZAP cDNA library was constructed from a
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/abbi.1993.1003" target="_blank" rel="noreferrer noopener">10.1006/abbi.1993.1003</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1993
2-Acetylaminofluorene
Aflatoxin B1
Amino Acid
Amino Acid Sequence
Animals
Antisense Elements (Genetics)
Archives of biochemistry and biophysics
Base Sequence
Blotting
Chen L
Clofibrate
Cytochrome P-450 Enzyme System/analysis/*genetics
Department of Integrative Medical Sciences
Hardwick J P
Humans
Liver Neoplasms/chemically induced/*enzymology/genetics
Male
Microsomes/enzymology
Molecular Sequence Data
Multigene Family
NEOMED College of Medicine
Northern
Rats
Sequence Homology
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jnr.10421" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jnr.10421</a>
Pages
808–816
Issue
6
Volume
70
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Estrogen receptor-alpha and neural circuits to the spinal cord during pregnancy.
Publisher
An entity responsible for making the resource available
Journal of neuroscience research
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-12
Subject
The topic of the resource
Afferent/*metabolism; Animal/*physiology; Animals; Autonomic/anatomy & histology/metabolism; Blotting; Estrogen Receptor alpha; Estrogen/*biosynthesis; Estrogens/*physiology; Female; Ganglia; Immunohistochemistry; Neurons; Parturition/physiology; Pregnancy; Proto-Oncogene Proteins c-fos/*biosynthesis; Rats; Receptors; Sensory/anatomy & histology/metabolism; Spinal Cord/anatomy & histology/metabolism; Sprague-Dawley; Time Factors; Uterus/innervation; Western
Creator
An entity primarily responsible for making the resource
Papka R E; Hafemeister J; Puder B A; Usip S; Storey-Workley M
Description
An account of the resource
Estrogen receptor immunoreactivity and mRNAs are present in spinal cord neurons in locations that are associated with sensory and autonomic innervation of female reproductive organs. The present study was undertaken to examine the expression of estrogen receptor-alpha in the spinal cord during different stages of pregnancy and to determine whether estrogen receptor-alpha-expressing neurons are related to uterine afferent nerves bringing information to the spinal cord at parturition. Immunohistochemistry showed estrogen receptor-alpha-immunoreactive neurons in the dorsal one-half of the spinal cord, i.e., dorsal horn, dorsal intermediate gray areas (dorsal commissural nucleus), and around the central canal and sacral parasympathetic autonomic nucleus of the lumbosacral spinal cord. Neurons in these areas corresponded topographically to the distribution of central processes of visceral primary afferent neurons (e.g., containing calcitonin gene-related peptide and substance P) that innervate and activate second-order spinal cord neurons (evidenced by their expression of Fos) at parturition. Western blots showed that estrogen receptor-alpha increases in the spinal cord, with a peak at day 20 of gestation, followed by a slight decrease by 2 days postpartum. These studies show that estrogen receptor-alpha is expressed by neurons in autonomic and sensory areas of the lumbosacral spinal cord that have connections with the female reproductive system and that the level of estrogen receptor-alpha changes over the course of pregnancy, which may follow profiles of steroid hormones. Many of these neurons may be involved in processing information related to reproductive organ function, changes during pregnancy, and relays to other CNS centers.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/jnr.10421" target="_blank" rel="noreferrer noopener">10.1002/jnr.10421</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2002
Afferent/*metabolism
Animal/*physiology
Animals
Autonomic/anatomy & histology/metabolism
Blotting
Department of Anatomy & Neurobiology
Estrogen Receptor alpha
Estrogen/*biosynthesis
Estrogens/*physiology
Female
Ganglia
Hafemeister J
Immunohistochemistry
Journal of neuroscience research
NEOMED College of Medicine
Neurons
Papka R E
Parturition/physiology
Pregnancy
Proto-Oncogene Proteins c-fos/*biosynthesis
Puder B A
Rats
Receptors
Sensory/anatomy & histology/metabolism
Spinal Cord/anatomy & histology/metabolism
Sprague-Dawley
Storey-Workley M
Time Factors
Usip S
Uterus/innervation
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jnr.10097" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jnr.10097</a>
Pages
346–353
Issue
3
Volume
67
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Comparative binding of biotinylated neurotrophins to alpha(2)-macroglobulin family of proteins: relationship between cytokine-binding and neuro-modulatory activities of the macroglobulins.
Publisher
An entity responsible for making the resource available
Journal of neuroscience research
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-02
Subject
The topic of the resource
*Biotinylation; *Blotting; alpha-Macroglobulins/chemistry/*metabolism/pharmacology; Animals; Cytokines/chemistry/*metabolism; Humans; Iodine Radioisotopes/chemistry; Nerve Growth Factor/chemistry/metabolism; Nerve Growth Factors/chemistry/*metabolism; Neurons/drug effects; Pregnancy Proteins/chemistry/metabolism; Protein Binding/physiology; Rats; Species Specificity; Western
Creator
An entity primarily responsible for making the resource
Skornicka Erin L; Shi Xiaoqing; Koo Peter H
Description
An account of the resource
Human alpha(2)-macroglobulin (alpha(2)M), pregnancy zone protein (PZP), rat alpha(1)M and acute-phase rat alpha(2)M belong to the alpha(2)M gene family of proteins, which can react covalently with nucleophilic monoamines to yield monoamine-activated (MA) macroglobulins. The MA forms of human alpha(2)M, PZP and rat alpha(2)M have been demonstrated previously to inhibit various neurotrophin-promoted neuronal activities, whereas MA-alpha(1)M is neurostimulatory and all native macroglobulins are generally inactive. The mechanism of neuromodulation is unknown, but it has been postulated that MA macroglobulins might inhibit neurons via their binding and sequestration of neurotrophins. This study employed a novel biotinylation-Western blot technique to compare the neurotrophin-binding properties of the four macroglobulins, and to correlate their binding activities with their known neuro-modulatory activities. In comparison with their respective native counterparts, human and rat
Identifier
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<a href="http://doi.org/10.1002/jnr.10097" target="_blank" rel="noreferrer noopener">10.1002/jnr.10097</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Biotinylation
*Blotting
2002
alpha-Macroglobulins/chemistry/*metabolism/pharmacology
Animals
Cytokines/chemistry/*metabolism
Humans
Iodine Radioisotopes/chemistry
Journal of neuroscience research
Koo Peter H
Nerve Growth Factor/chemistry/metabolism
Nerve Growth Factors/chemistry/*metabolism
Neurons/drug effects
Pregnancy Proteins/chemistry/metabolism
Protein Binding/physiology
Rats
Shi Xiaoqing
Skornicka Erin L
Species Specificity
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jcp.22704" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jcp.22704</a>
Pages
70–76
Issue
1
Volume
227
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Primary cilia regulates the directional migration and barrier integrity of endothelial cells through the modulation of hsp27 dependent actin cytoskeletal organization.
Publisher
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Journal of cellular physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-01
Subject
The topic of the resource
*Cell Movement; Actin Cytoskeleton/*metabolism; Animals; Blotting; Capillary Permeability/*physiology; Cell Adhesion; Cilia/metabolism; Endothelial Cells/cytology/*metabolism; Fluorescent Antibody Technique; Focal Adhesions/metabolism; HSP27 Heat-Shock Proteins/*metabolism; Mice; Polycystic Kidney Diseases/physiopathology; Signal Transduction/physiology; Transgenic; Western
Creator
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Jones Thomas J; Adapala Ravi K; Geldenhuys Werner J; Bursley Chris; AbouAlaiwi Wissam A; Nauli Surya M; Thodeti Charles K
Description
An account of the resource
Cilia are mechanosensing organelles that communicate extracellular signals into intracellular responses. Altered functions of primary cilia play a key role in the development of various diseases including polycystic kidney disease. Here, we show that endothelial cells from the oak ridge polycystic kidney (Tg737(orpk/orpk) ) mouse, with impaired cilia assembly, exhibit a reduction in the actin stress fibers and focal adhesions compared to wild-type (WT). In contrast, endothelial cells from polycystin-1 deficient mice (pkd1(null/null) ), with impaired cilia function, display robust stress fibers, and focal adhesion assembly. We found that the Tg737(orpk/orpk) cells exhibit impaired directional migration and endothelial cell monolayer permeability compared to the WT and pkd1(null/null) cells. Finally, we found that the expression of heat shock protein 27 (hsp27) and the phosphorylation of focal adhesion kinase (FAK) are downregulated in the Tg737(orpk/orpk) cells and overexpression of hsp27 restored both FAK phosphorylation and cell migration. Taken together, these results demonstrate that disruption of the primary cilia structure or function compromises the endothelium through the suppression of hsp27 dependent actin organization and focal adhesion formation, which may contribute to the vascular dysfunction in ciliopathies.
Identifier
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<a href="http://doi.org/10.1002/jcp.22704" target="_blank" rel="noreferrer noopener">10.1002/jcp.22704</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Cell Movement
2012
AbouAlaiwi Wissam A
Actin Cytoskeleton/*metabolism
Adapala Ravi K
Animals
Blotting
Bursley Chris
Capillary Permeability/*physiology
Cell Adhesion
Cilia/metabolism
Department of Integrative Medical Sciences
Endothelial Cells/cytology/*metabolism
Fluorescent Antibody Technique
Focal Adhesions/metabolism
Geldenhuys Werner J
HSP27 Heat-Shock Proteins/*metabolism
Jones Thomas J
Journal of cellular physiology
Mice
Nauli Surya M
NEOMED College of Medicine
Polycystic Kidney Diseases/physiopathology
Signal Transduction/physiology
Thodeti Charles K
Transgenic
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jcb.23025" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jcb.23025</a>
Pages
1118–1129
Issue
4
Volume
112
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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The presence of extracellular matrix alters the chondrocyte response to endoplasmic reticulum stress.
Publisher
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Journal of cellular biochemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-04
Subject
The topic of the resource
*Stress; Animals; Apoptosis/drug effects; Articular/cytology; Blotting; Cartilage; Cattle; Cells; Chondrocytes/cytology/drug effects/*metabolism; Cultured; DNA-Binding Proteins/genetics/metabolism; Dose-Response Relationship; Drug; Endoplasmic Reticulum/*metabolism; Extracellular Matrix/*metabolism; Glucose/pharmacology; Heat-Shock Proteins/genetics/metabolism; Physiological; Proto-Oncogene Proteins c-bcl-2/genetics/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Thapsigargin/pharmacology; Time Factors; Transcription Factors/genetics/metabolism; Tunicamycin/pharmacology; Western
Creator
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Nugent Ashleigh E; McBurney Denise L; Horton Walter E Jr
Description
An account of the resource
The objective of this study was to test the hypothesis that extracellular matrix (ECM) would alter the endoplasmic reticulum (ER) stress response of chondrocytes. Chondrocytes were isolated from calf knees and maintained in monolayer culture or suspended in collagen I to form spot cultures (SCs). Our laboratory has shown that bovine chondrocytes form cartilage with properties similar to native cartilage after 2-4 weeks in SCs. Monolayer cultures treated with ER stressors glucose withdrawal (-Glu), tunicamycin (TN), or thapsigargin (TG) up-regulated Grp78 and Gadd153, demonstrating a complete ER stress response. SCs were grown at specific times from 1 day to 6 weeks before treatment with ER stressors. Additionally, SCs grown for 1, 2, or 6 weeks were treated with increasing concentrations of TN or TG. Western blotting of SCs for Grp78 indicated that increased ECM accumulation results in delayed expression; however, Grp78 mRNA is up-regulated in response to ER stressors even after 6 weeks in culture. SCs treated with ER stressors did not up-regulate Gadd153, suggesting that the cells experienced ER stress but would not undergo apoptosis. In fact, SCs undergo apoptosis upon ER stress treatment after 0-1 day of growth; however, after 4 days and to 6 weeks, apoptosis in treated samples was not different than controls. Pro-survival molecules Bcl-2 and Bag-1 were up-regulated upon ER stress in SCs. These results suggest that presence of ECM confers protection from ER stressors. Future studies involving chondrocyte physiology should focus on responses in conditions more closely mimicking the in vivo cartilage environment.
Identifier
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<a href="http://doi.org/10.1002/jcb.23025" target="_blank" rel="noreferrer noopener">10.1002/jcb.23025</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Stress
2011
Animals
Apoptosis/drug effects
Articular/cytology
Blotting
Cartilage
Cattle
Cells
Chondrocytes/cytology/drug effects/*metabolism
Cultured
Department of Anatomy & Neurobiology
DNA-Binding Proteins/genetics/metabolism
Dose-Response Relationship
Drug
Endoplasmic Reticulum/*metabolism
Extracellular Matrix/*metabolism
Glucose/pharmacology
Heat-Shock Proteins/genetics/metabolism
Horton Walter E Jr
Journal of cellular biochemistry
McBurney Denise L
NEOMED College of Medicine
Nugent Ashleigh E
Physiological
Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
Reverse Transcriptase Polymerase Chain Reaction
Thapsigargin/pharmacology
Time Factors
Transcription Factors/genetics/metabolism
Tunicamycin/pharmacology
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/art.38952" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/art.38952</a>
Pages
423–434
Issue
2
Volume
67
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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MicroRNA-602 and microRNA-608 regulate sonic hedgehog expression via target sites in the coding region in human chondrocytes.
Publisher
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Arthritis & rheumatology (Hoboken, N.J.)
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015-02
Subject
The topic of the resource
Academic Medical Centers; Aged; Analysis of Variance; Animal; Animals; Anterior Cruciate Ligament/surgery; Blotting; Cells; Chondrocytes – Physiology; Chondrocytes/drug effects/*metabolism/pathology; Cultured; Disease Models; Female; Funding Source; Gene Expression Regulation/genetics/*physiology; Hedgehog Proteins/genetics/*metabolism; HEK293 Cells; Human; Humans; Immunohistochemistry; In Vitro Techniques; Interleukin-1beta/pharmacology; Knee/etiology/*metabolism/pathology; Male; Matrix Metalloproteinase 13/metabolism; MicroRNAs/genetics/*metabolism; Middle Age; Middle Aged; Ohio; Open Reading Frames/genetics/*physiology; Osteoarthritis; Osteoarthritis – Epidemiology; Osteoarthritis – Physiopathology; Polymerase Chain Reaction; Rabbits; Signal Transduction/genetics/physiology; T-Tests; Transfection; Up-Regulation/drug effects/genetics/physiology; Western
Creator
An entity primarily responsible for making the resource
Akhtar Nahid; Makki Mohammad S; Haqqi Tariq M
Description
An account of the resource
OBJECTIVE: Hedgehog (HH) signaling has recently been associated with cartilage degradation in osteoarthritis (OA). Because interleukin-1beta (IL-1beta) has been implicated as a principal instigator of OA, we sought to determine whether
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/art.38952" target="_blank" rel="noreferrer noopener">10.1002/art.38952</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2015
Academic Medical Centers
Aged
Akhtar Nahid
Analysis of Variance
Animal
Animals
Anterior Cruciate Ligament/surgery
Arthritis & rheumatology (Hoboken, N.J.)
Blotting
Cells
Chondrocytes – Physiology
Chondrocytes/drug effects/*metabolism/pathology
Cultured
Department of Anatomy & Neurobiology
Disease Models
Female
Funding Source
Gene Expression Regulation/genetics/*physiology
Haqqi Tariq M
Hedgehog Proteins/genetics/*metabolism
HEK293 Cells
Human
Humans
Immunohistochemistry
In Vitro Techniques
Interleukin-1beta/pharmacology
Knee/etiology/*metabolism/pathology
Makki Mohammad S
Male
Matrix Metalloproteinase 13/metabolism
MicroRNAs/genetics/*metabolism
Middle Age
Middle Aged
NEOMED College of Medicine
Ohio
Open Reading Frames/genetics/*physiology
Osteoarthritis
Osteoarthritis – Epidemiology
Osteoarthritis – Physiopathology
Polymerase Chain Reaction
Rabbits
Signal Transduction/genetics/physiology
T-Tests
Transfection
Up-Regulation/drug effects/genetics/physiology
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/ar.21363" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/ar.21363</a>
Pages
1461–1471
Issue
9
Volume
294
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Fetal lung epithelial ion channels relocate in the cell membrane during late gestation.
Publisher
An entity responsible for making the resource available
Anatomical record (Hoboken, N.J. : 2007)
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-09
Subject
The topic of the resource
Animals; Blotting; Caveolin 1/*metabolism; Cell Membrane/*metabolism; Cl transport; Cystic Fibrosis Transmembrane Conductance Regulator/*metabolism; distal lung fluid absorption; Epithelial Sodium Channels/*metabolism; Epithelium/embryology/*metabolism; Female; fetal lung development; Fetus/*metabolism; Gestational Age; Guinea Pigs; IL-1beta; Immunoprecipitation; Interleukin-1beta/metabolism; Ion Channels/metabolism; Lung/embryology/*metabolism; Na transport; Western
Creator
An entity primarily responsible for making the resource
Beard LaMonta L; Li Tianbo; Hu Yang; Folkesson Hans G
Description
An account of the resource
Near the end of gestation, the direction of ion and fluid flow across the alveolar epithelium rapidly changes from secretion to absorption. Thus, the relative cell membrane location of epithelial Na channels (ENaCs) and cystic fibrosis transmembrane regulator (CFTR) Cl channels during late fetal lung development and after maternal interleukin-1beta (IL-1beta) pretreatment was the focus of our study. Western blot analysis after sucrose gradient separation of caveolin-1-(Cav-1)-rich membrane regions (CRR) and Cav-1-poor membrane (non-CRR) revealed primary CRR ENaC localization at gestation day (GD) 61 in guinea pigs. Correlating with the natural induction of distal lung fluid absorption, ENaC appeared in the non-CRR cell membrane regions at GD68. Conversely, CFTR was present in the non-CRR cell membrane regions at GD61 and in the CRRs at GD68.
Identifier
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<a href="http://doi.org/10.1002/ar.21363" target="_blank" rel="noreferrer noopener">10.1002/ar.21363</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2011
Anatomical record (Hoboken, N.J. : 2007)
Animals
Beard LaMonta L
Blotting
Caveolin 1/*metabolism
Cell Membrane/*metabolism
Cl transport
Cystic Fibrosis Transmembrane Conductance Regulator/*metabolism
distal lung fluid absorption
Epithelial Sodium Channels/*metabolism
Epithelium/embryology/*metabolism
Female
fetal lung development
Fetus/*metabolism
Folkesson Hans G
Gestational Age
Guinea Pigs
Hu Yang
IL-1beta
Immunoprecipitation
Interleukin-1beta/metabolism
Ion Channels/metabolism
Li Tianbo
Lung/embryology/*metabolism
Na transport
Western