1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1097/ALN.0b013e31820dee67" target="_blank" rel="noreferrer noopener">http://doi.org/10.1097/ALN.0b013e31820dee67</a> Pages 1169–1179 Issue 5 Volume 114 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Propofol restores transient receptor potential vanilloid receptor subtype-1 sensitivity via activation of transient receptor potential ankyrin receptor subtype-1 in sensory neurons. Publisher An entity responsible for making the resource available Anesthesiology Date A point or period of time associated with an event in the lifecycle of the resource 2011 2011-05 Subject The topic of the resource Male; Mice; Capsaicin; Cells; Anesthetics; Animal Studies; Genes – Drug Effects; Carrier Proteins – Drug Effects; Carrier Proteins – Metabolism; Intravenous – Pharmacodynamics; Propofol – Pharmacodynamics; Sensory Receptor Cells – Drug Effects Creator An entity primarily responsible for making the resource Zhang H; Wickley PJ; Sinha S; Bratz IN; Damron DS; Zhang Hongyu; Wickley Peter J; Sinha Sayantani; Bratz Ian N; Damron Derek S Description An account of the resource Background: Cross talk between peripheral nociceptors belonging to the transient receptor potential vanilloid receptor subtype-1 (TRPV1) and ankyrin subtype-1 (TRPA1) family has been demonstrated recently. Moreover, the intravenous anesthetic propofol has directly activates TRPA1 receptors and indirectly restores sensitivity of TRPV1 receptors in dorsal root ganglion (DRG) sensory neurons. Our objective was to determine the extent to which TRPA1 activation is involved in mediating the propofol-induced restoration of TRPV1 sensitivity.Methods: Mouse DRG neurons were isolated by enzymatic dissociation and grown for 24 h. F-11 cells were transfected with complementary DNA for both TRPV1 and TRPA1 or TRPV1 only. The intracellular Ca concentration was measured in individual cells via fluorescence microscopy. After TRPV1 desensitization with capsaicin (100 nM), cells were treated with propofol (1, 5, and 10 μM) alone or with propofol in the presence of the TRPA1 antagonist, HC-030031 (0.5 μM), or the TRPA1 agonist, allyl isothiocyanate (AITC; 100 μM); capsaicin was then reapplied.Results: In DRG neurons that contain both TRPV1 and TRPA1, propofol and AITC restored TRPV1 sensitivity. However, in DRG neurons containing only TRPV1 receptors, exposure to propofol or AITC after desensitization did not restore capsaicin-induced TRPV1 sensitivity. Similarly, in F-11 cells transfected with both TRPV1 and TRPA1, propofol and AITC restored TRPV1 sensitivity. However, in F-11 cells transfected with TRPV1 only, neither propofol nor AITC was capable of restoring TRPV1 sensitivity.Conclusions: These data demonstrate that propofol restores TRPV1 sensitivity in primary DRG neurons and in cultured F-11 cells transfected with both the TRPV1 and TRPA1 receptors via a TRPA1-dependent process. Propofol's effects on sensory neurons may be clinically important and may contribute to peripheral sensitization to nociceptive stimuli in traumatized tissue. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1097/ALN.0b013e31820dee67" target="_blank" rel="noreferrer noopener">10.1097/ALN.0b013e31820dee67</a> Rights Information about rights held in and over the resource Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). 2011 Anesthesiology Anesthetics Animal Studies Bratz Ian N Bratz IN Capsaicin Carrier Proteins – Drug Effects Carrier Proteins – Metabolism Cells Damron Derek S Damron DS Genes – Drug Effects Intravenous – Pharmacodynamics Male Mice Propofol – Pharmacodynamics Sensory Receptor Cells – Drug Effects Sinha S Sinha Sayantani Wickley Peter J Wickley PJ Zhang H Zhang Hongyu