1
40
2
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.dib.2017.03.054" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.dib.2017.03.054</a>
Pages
150–155
Volume
12
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Dataset of effect of Wogonin, a natural flavonoid, on the viability and activation of NF-kappaB and MAPKs in IL-1beta-stimulated human OA chondrocytes.
Publisher
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Data in brief
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-06
Subject
The topic of the resource
MAPK; Mass-spectrometry; NF-kappaB; Nrf2; Osteoarthritis; Wogonin
Creator
An entity primarily responsible for making the resource
Khan Nazir M; Haseeb Abdul; Ansari Mohammad Y; Devarapalli Pratap; Haynie Sara; Haqqi Tariq M
Description
An account of the resource
This article contains data related to the article "Wogonin, a plant derived small molecule exerts potent anti-inflammatory and chondroprotective effects through activation of ROS/ERK/Nrf2 signaling pathways in human Osteoarthritis chondrocytes" (Khan et al. 2017) [1]. The data are related to effects of Wogonin on the viability and IL-1beta-stimulated activation of NF-kappaB and ERK1/2, JNK1/2 and p38 MAPKs in human OA chondrocytes. Gene expression data representing the chondrogenic phenotype and the efficiency of Nrf2 knockdown in monolayer culture of human OA chondrocytes were shown. Moreover, mass spectrometric calibration curve of Wogonin used to quantify the intracellular uptake were also presented. The data are presented in the form of figures and significance of these has been given in the research article (Khan et al. 2017) [1].
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.dib.2017.03.054" target="_blank" rel="noreferrer noopener">10.1016/j.dib.2017.03.054</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Ansari Mohammad Y
Data in brief
Department of Anatomy & Neurobiology
Devarapalli Pratap
Haqqi Tariq M
Haseeb Abdul
Haynie Sara
Khan Nazir M
MAPK
Mass-spectrometry
NEOMED College of Medicine
NF-kappaB
Nrf2
Osteoarthritis
Wogonin
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.cbi.2017.06.025" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.cbi.2017.06.025</a>
Pages
13–23
Volume
274
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Wogonin, a natural flavonoid, intercalates with genomic DNA and exhibits protective effects in IL-1beta stimulated osteoarthritis chondrocytes.
Publisher
An entity responsible for making the resource available
Chemico-biological interactions
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-08
Subject
The topic of the resource
Apoptosis/drug effects; Binding Sites; Cells; Chondrocytes/cytology/*drug effects/metabolism; Chondroprotective effects; Cultured; Denaturation; DNA binding; DNA/chemistry/*metabolism; Flavanones/chemistry/metabolism/*pharmacology; Flavonoids/chemistry/pharmacology; Fluorescence Resonance Energy Transfer; Humans; Intercalating Agents/chemistry/metabolism/*pharmacology; Interleukin-1beta/*pharmacology; Molecular Docking Simulation; Nucleic Acid Conformation; Osteoarthritis; Osteoarthritis/metabolism/pathology; Protective Agents/chemistry/metabolism/*pharmacology; Reactive Oxygen Species/metabolism; Up-Regulation/drug effects; Wogonin
Creator
An entity primarily responsible for making the resource
Khan Nazir M; Ahmad Imran; Ansari Mohammad Y; Haqqi Tariq M
Description
An account of the resource
Wogonin has recently been shown to possess anti-inflammatory and chondroprotective properties and is of considerable interest due to its broad pharmacological activities. The present study highlights that Wogonin binds DNA and exerts chondroprotective effects in vitro. Wogonin showed strong binding with chondrocytes genomic DNA in vitro. The mode of binding of Wogonin to genomic-DNA was assessed by competing Wogonin with EtBr or DAPI, known DNA intercalator and a minor groove binder, respectively. EtBr fluorescence reduced significantly with increase in Wogonin concentration suggesting possible DNA intercalation of Wogonin. Further, in silico molecular docking of Wogonin on mammalian DNA also indicated possible intercalation of Wogonin with DNA. The denaturation and FRET studies revealed that Wogonin prevents denaturation of DNA strands and provide stability to genomic DNA against a variety of chemical denaturants. The cellular uptake study showed that Wogonin enters osteoarthritis chondrocytes and was mainly localized in the nucleus. Wogonin treatment to OA chondrocytes protects the fragmentation of genomic DNA in response to IL-1beta as evaluated by DNA ladder and TUNEL assay. Treatment of chondrocytes with Wogonin resulted in significant suppression of IL-1beta-mediated induction of ROS. Further, Wogonin exhibited protective potential through potent suppression of extrinsic and intrinsic apoptotic pathways and induction of anti-apoptotic proteins in
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.cbi.2017.06.025" target="_blank" rel="noreferrer noopener">10.1016/j.cbi.2017.06.025</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Ahmad Imran
Ansari Mohammad Y
Apoptosis/drug effects
Binding Sites
Cells
Chemico-biological interactions
Chondrocytes/cytology/*drug effects/metabolism
Chondroprotective effects
Cultured
Denaturation
Department of Anatomy & Neurobiology
DNA binding
DNA/chemistry/*metabolism
Flavanones/chemistry/metabolism/*pharmacology
Flavonoids/chemistry/pharmacology
Fluorescence Resonance Energy Transfer
Haqqi Tariq M
Humans
Intercalating Agents/chemistry/metabolism/*pharmacology
Interleukin-1beta/*pharmacology
Khan Nazir M
Molecular Docking Simulation
NEOMED College of Medicine
Nucleic Acid Conformation
Osteoarthritis
Osteoarthritis/metabolism/pathology
Protective Agents/chemistry/metabolism/*pharmacology
Reactive Oxygen Species/metabolism
Up-Regulation/drug effects
Wogonin