1
40
3
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M502751200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M502751200</a>
Pages
30517–30525
Issue
34
Volume
280
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bcl-2 positively regulates Sox9-dependent chondrocyte gene expression by suppressing the MEK-ERK1/2 signaling pathway.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-08
Subject
The topic of the resource
*Gene Expression Regulation; Adenoviridae/genetics; Animals; Apoptosis; beta-Galactosidase/metabolism; Blotting; Butadienes/pharmacology; Caspase Inhibitors; Cell Differentiation; Cell Line; Chondrocytes/*metabolism; Collagen Type II/metabolism; Down-Regulation; Enzyme Inhibitors/pharmacology; Fibroblasts/metabolism; Fluorescence; Genetic; High Mobility Group Proteins/*metabolism; Lac Operon; Luciferases/metabolism; MAP Kinase Kinase Kinases/*metabolism; Messenger/metabolism; Microscopy; Mitogen-Activated Protein Kinase 1/*metabolism; Mitogen-Activated Protein Kinase 3/*metabolism; NF-kappa B/metabolism; Nitriles/pharmacology; Phenotype; Phosphorylation; Promoter Regions; Protein Kinase C-alpha; Protein Kinase C/antagonists & inhibitors; Proteoglycans/metabolism; Proto-Oncogene Proteins c-bcl-2/*metabolism; Rats; Reverse Transcriptase Polymerase Chain Reaction; RNA; Signal Transduction; Small Interfering/metabolism; SOX9 Transcription Factor; Sprague-Dawley; Time Factors; Transcription; Transcription Factors/*metabolism; Transfection; Western
Creator
An entity primarily responsible for making the resource
Yagi Rieko; McBurney Denise; Horton Walter E Jr
Description
An account of the resource
Bcl-2 is an anti-apoptotic protein that has recently been shown to regulate other cellular functions. We previously reported that Bcl-2 regulates chondrocyte matrix gene expression, independent of its anti-apoptotic function. Here, we further investigate this novel function of Bcl-2 and examine three intracellular signaling pathways likely to be associated with this function. The present study demonstrates that the activity of Sox9, a master transcription factor that regulates the gene expression of chondrocyte matrix proteins, is suppressed by Bcl-2 small interference RNA in the presence of caspase inhibitors. This effect was attenuated by prior exposure of chondrocytes to an adenoviral vector expressing sense Bcl-2. In addition, the down-regulation of Bcl-2, Sox9, and chondrocyte-specific gene expression by serum withdrawal in primary chondrocytes was reversed by expressing Bcl-2. Inhibition of the protein kinase C alpha and NFkappaB pathways had no effect on the maintenance of Sox9-dependent gene expression by Bcl-2. In contrast, whereas the MEK-ERK1/2 pathway negatively regulated the differentiated phenotype in wild type chondrocytes, inhibition of this pathway reversed the loss of differentiation markers and fibroblastic phenotype in Bcl-2-deficient chondrocytes. In conclusion, the present study identifies a specific signaling pathway, namely, MEK-ERK1/2, that is downstream of Bcl-2 in the regulation of Sox9-dependent chondrocyte gene expression and phenotype.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M502751200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M502751200</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
2005
Adenoviridae/genetics
Animals
Apoptosis
beta-Galactosidase/metabolism
Blotting
Butadienes/pharmacology
Caspase Inhibitors
Cell Differentiation
Cell Line
Chondrocytes/*metabolism
Collagen Type II/metabolism
Department of Anatomy & Neurobiology
Down-Regulation
Enzyme Inhibitors/pharmacology
Fibroblasts/metabolism
Fluorescence
Genetic
High Mobility Group Proteins/*metabolism
Horton Walter E Jr
Lac Operon
Luciferases/metabolism
MAP Kinase Kinase Kinases/*metabolism
McBurney Denise
Messenger/metabolism
Microscopy
Mitogen-Activated Protein Kinase 1/*metabolism
Mitogen-Activated Protein Kinase 3/*metabolism
NEOMED College of Medicine
NF-kappa B/metabolism
Nitriles/pharmacology
Phenotype
Phosphorylation
Promoter Regions
Protein Kinase C-alpha
Protein Kinase C/antagonists & inhibitors
Proteoglycans/metabolism
Proto-Oncogene Proteins c-bcl-2/*metabolism
Rats
Reverse Transcriptase Polymerase Chain Reaction
RNA
Signal Transduction
Small Interfering/metabolism
SOX9 Transcription Factor
Sprague-Dawley
The Journal of biological chemistry
Time Factors
Transcription
Transcription Factors/*metabolism
Transfection
Western
Yagi Rieko
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.orthres.2004.12.016" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.orthres.2004.12.016</a>
Pages
1128–1138
Issue
5
Volume
23
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Intrajoint comparisons of gene expression patterns in human osteoarthritis suggest a change in chondrocyte phenotype.
Publisher
An entity responsible for making the resource available
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-09
Subject
The topic of the resource
*Gene Expression Profiling; 80 and over; Aged; Articular/*metabolism; Cartilage; Chondrocytes/*metabolism; Collagen Type II/genetics; Glycosaminoglycans/analysis; High Mobility Group Proteins/genetics; Humans; Matrix Metalloproteinase 3/genetics; Matrix Metalloproteinase 9/genetics; Messenger/analysis; Osteoarthritis/*metabolism; Phenotype; Proto-Oncogene Proteins c-bcl-2/genetics; RNA; SOX9 Transcription Factor; Transcription Factors/genetics
Creator
An entity primarily responsible for making the resource
Yagi Rieko; McBurney Denise; Laverty David; Weiner Scott; Horton Walter E Jr
Description
An account of the resource
Osteoarthritis (OA) is a degenerative cartilage disease with varying degrees of severity within a given joint. The purpose of this study was to define a sampling procedure for comparing human minimal and advanced OA cartilage in the same patient and to determine basic patterns of gene expression in these regions. A specific hypothesis under study was that the expression level of Bcl-2 would correlate with Sox9 and aggrecan mRNA expression in vivo as has been demonstrated in vitro. Femoral condylar advanced OA cartilage was located within 1cm of overt lesions, and minimal cartilage was taken from areas with no obvious surface defects. Histological sections were scored for disease severity and chondroitin sulfate and hydroxyproline content was determined. The expression level of nine specific genes (aggrecan, collagen type II, Bcl-2, Sox9, Link protein, osteopontin, and MMP-13, -3, and -9) was determined by quantitative real time PCR. The scores for fibrillation, chondrocyte cloning, and proteoglycan depletion were significantly different between advanced and minimal OA cartilage. The advanced OA cartilage had significantly less chondroitin sulfate than the minimal OA cartilage. Osteopontin mRNA expression showed a 3.6-fold increase in advanced compared to minimal OA cartilage. In contrast, the level of mRNA coding for aggrecan, link protein, Bcl-2, Sox9 and MMP-3, -9, -13 were all decreased in advanced compared to minimal cartilage in the majority of the patients studied. Collagen type II mRNA expression displayed a wide-range of variation. A statistically significant correlation was observed both between Bcl-2 and Sox9 mRNA level, and between Bcl-2 and aggrecan mRNA expression. The patient matched comparison of minimal and advanced OA cartilage revealed differences in cellular and tissue characteristics, and changes in gene expression that may be involved in OA progression. In addition, Bcl-2 may also play a role in regulating the expression of aggrecan through Sox9 in vivo as well as in vitro.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.orthres.2004.12.016" target="_blank" rel="noreferrer noopener">10.1016/j.orthres.2004.12.016</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Profiling
2005
80 and over
Aged
Articular/*metabolism
Cartilage
Chondrocytes/*metabolism
Collagen Type II/genetics
Department of Anatomy & Neurobiology
Glycosaminoglycans/analysis
High Mobility Group Proteins/genetics
Horton Walter E Jr
Humans
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Laverty David
Matrix Metalloproteinase 3/genetics
Matrix Metalloproteinase 9/genetics
McBurney Denise
Messenger/analysis
NEOMED College of Medicine
Osteoarthritis/*metabolism
Phenotype
Proto-Oncogene Proteins c-bcl-2/genetics
RNA
SOX9 Transcription Factor
Transcription Factors/genetics
Weiner Scott
Yagi Rieko
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/ar.a.20063" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/ar.a.20063</a>
Pages
720–728
Issue
2
Volume
279
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Age-related expression patterns of Bag-1 and Bcl-2 in growth plate and articular chondrocytes.
Publisher
An entity responsible for making the resource available
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-08
Subject
The topic of the resource
Aging/*metabolism; Animals; Articular/cytology/*metabolism; Carrier Proteins/genetics/*metabolism; Cartilage; Cell Division/physiology; Cells; Chondrocytes/cytology/*metabolism; Cultured; DNA-Binding Proteins; Gene Expression Regulation; Growth Plate/*metabolism; Inbred C57BL; Mice; Proto-Oncogene Proteins c-bcl-2/*metabolism; Tissue Distribution; Transcription Factors
Creator
An entity primarily responsible for making the resource
Kinkel Mary D; Yagi Rieko; McBurney Denise; Nugent Ashleigh; Horton Walter E Jr
Description
An account of the resource
Aging cartilage displays increased chondrocyte apoptosis and decreased responsiveness of chondrocytes to growth factors. The molecular mechanisms responsible for these changes have not been identified. Bag-1 is a Bcl-2-binding protein that promotes cell survival, interacts with a diverse group of cellular proteins, and may integrate multiple pathways involved in controlling cell survival, growth, and phenotype. Bcl-2 is important for maintaining chondrocyte phenotype and delaying terminal differentiation and apoptosis of chondrocytes. Comparatively little is known about the role of Bag-1 in cartilage. Here we show that both growth plate and articular chondrocytes in the mouse express the Bag-1 protein. In the growth plate, Bag-1 expression is prominent in the late proliferative and prehypertrophic chondrocytes, displaying a pattern similar to what has been reported for Bcl-2. Further, the expression of both Bcl-2 and Bag-1 declines with age in the articular cartilage. Growth assays demonstrate that knocking down Bag-1 expression causes a decrease in growth rate. These results suggest that Bag-1 is involved in the regulation of chondrocyte phenotype and cartilage aging.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/ar.a.20063" target="_blank" rel="noreferrer noopener">10.1002/ar.a.20063</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2004
Aging/*metabolism
Animals
Articular/cytology/*metabolism
Carrier Proteins/genetics/*metabolism
Cartilage
Cell Division/physiology
Cells
Chondrocytes/cytology/*metabolism
Cultured
Department of Anatomy & Neurobiology
DNA-Binding Proteins
Gene Expression Regulation
Growth Plate/*metabolism
Horton Walter E Jr
Inbred C57BL
Kinkel Mary D
McBurney Denise
Mice
NEOMED College of Medicine
Nugent Ashleigh
Proto-Oncogene Proteins c-bcl-2/*metabolism
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
Tissue Distribution
Transcription Factors
Yagi Rieko