Cloning and 5'-flanking sequence of a rat cholesterol 7 alpha-hydroxylase gene.
Creator
Chiang J Y; Yang T P; Wang D P
Publisher
Biochimica et biophysica acta
Date
1992
1992-10
Description
A cholesterol 7 alpha-hydroxylase gene containing 8 kb of the 5'-flanking region and 5 kb of the transcription region which covers exons 1 to 5 was isolated from a rat genomic library. The 2015 bp nucleotide sequence 5'-upstream from the start codon was determined. This promotor region contains many liver-enriched or -specific elements (TGT3, HNF/LF-B1), putative hormone responsive elements (TRE, GRE, RRE or RARE) and ubiquitous transcription factor binding sites (NF-1,
Subject
Animals; Rats; Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Cholesterol 7-alpha-Hydroxylase/*genetics; DNA; Restriction Mapping; Genetic; Cloning; Molecular; *Promoter Regions
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Nucleotide sequence of a region duplicated in Escherichia coli toc mutants.
Creator
Yang T P; Depew R E
Publisher
Biochimica et biophysica acta
Date
1992
1992-03
Description
We have sequenced about 5 kb of the Escherichia coli chromosome downstream from the tolC gene, looking for a topoisomerase gene. This region does not contain a topoisomerase gene.
Subject
*Genes; *Multigene Family; Bacterial; Base Sequence; DNA Topoisomerases; Escherichia coli/*genetics; Molecular Sequence Data; Type I/genetics