Isolation and characterization of cDNA clones for human, bovine and rabbit liver cytochrome b5 mRNA's.
Humans; Animals; Amino Acid Sequence; Rabbits; Base Sequence; Molecular Sequence Data; Liver/*enzymology; Cattle; Chickens; Cytochromes b5/blood/*genetics; DNA/*isolation & purification; Erythrocytes/enzymology; RNA; Sequence Homology; Cloning; Molecular; Nucleic Acid; Messenger/*genetics
Cristiano R J; Yoo M; Dariush N; Steggles A W
Progress in clinical and biological research
1989
1905-06
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
THE CHARACTERIZATION OF 3 TYPES OF PARTIALLY PROCESSED MESSENGER-RNA AND 2 PSEUDOGENES FOR HUMAN-LIVER CYTOCHROME-B5
Biochemistry & Molecular Biology; Biophysics
Yoo M; Steggles A W
Biochemical and Biophysical Research Communications
1989
1989-08
Journal Article
<a href="http://doi.org/10.1016/0006-291x(89)92092-5" target="_blank" rel="noreferrer noopener">10.1016/0006-291x(89)92092-5</a>
The characterization of three types of partially processed mRNA and two pseudogenes for human liver cytochrome b5.
Humans; Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Liver/*physiology; DNA/genetics; Cytochrome b Group/*genetics; Cytochromes b5; Pseudogenes; Cloning; Molecular; Post-Transcriptional; RNA Processing
We have isolated cDNA clones corresponding to partially processed human liver cytochrome b5 mRNAs. All the clones contained poly(A) sequences, and one clone had a shorter 3' non-translated sequence, indicating the use of an alternative poly(A) addition signal. In addition, all the clones contained the coding information for amino acids 87-134; however, there were two types of intron junction adjacent to the coding sequence. Detailed analysis of the Type I clones showed that the Type II intron sequence was contained within the Type I sequence, but approximately 1000 bp 5' of the Type I intron-exon junction showed alternative splicing within this intron. In addition, we have isolated two pseudogenes which lack introns, suggesting the retroviral insertion of human liver cytochrome b5 mRNA sequences into the human genome.
Yoo M; Steggles A W
Biochemical and biophysical research communications
1989
1989-08
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0006-291x(89)92092-5" target="_blank" rel="noreferrer noopener">10.1016/0006-291x(89)92092-5</a>
The complete nucleotide sequence of human liver cytochrome b5 mRNA.
Humans; Amino Acid Sequence; Liver/*metabolism; Base Sequence; Molecular Sequence Data; DNA/genetics; Cytochrome b Group/*genetics; Cytochromes b5; RNA; Cloning; Molecular; Messenger/*genetics
We have isolated and sequenced a cDNA clone corresponding to human liver cytochrome b5 mRNA. The 760 base pair (bp) sequence contains the complete coding and 3' non-translated regions plus 52 bp of 5' non-translated sequence. The derived amino acid sequence showed that the previous assignment of several amino acids was in error. In addition, the sequence of the previously unknown COOH hydrophobic region has been obtained.
Yoo M; Steggles A W
Biochemical and biophysical research communications
1988
1988-10
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/s0006-291x(88)80881-7" target="_blank" rel="noreferrer noopener">10.1016/s0006-291x(88)80881-7</a>
THE COMPLETE NUCLEOTIDE-SEQUENCE OF HUMAN-LIVER CYTOCHROME-B5 MESSENGER-RNA
Biochemistry & Molecular Biology; Biophysics
Yoo M; Steggles A W
Biochemical and Biophysical Research Communications
1988
1988-10
Journal Article
<a href="http://doi.org/10.1016/s0006-291x(88)80881-7" target="_blank" rel="noreferrer noopener">10.1016/s0006-291x(88)80881-7</a>
The human cytochrome b5 gene and two of its pseudogenes are located on chromosomes 18q23, 14q31-32.1 and 20p11.2, respectively.
*Chromosomes; Animals; Blotting; Cell Line; Chromosome Mapping; Cricetinae; Cytochromes b5/*genetics; DNA/analysis; Genetic/genetics; Human; Humans; Hybrid Cells; In Situ Hybridization; Molecular Sequence Data; Pair 14; Pair 18; Pair 20; Polymerase Chain Reaction; Pseudogenes/*genetics; Southern; Translocation
Using very high stringency hybridization conditions for the Southern blot hybridization analysis of hamster-human cell hybrid DNA, we were able to map the human cytochrome b5 gene and two of its pseudogenes (psgb(5)1 and psgb(5)2) unambiguously to chromosomes 18, 14, and 20. These localizations were confirmed and extended to 18q23, 14q31-32.1, and 20p11.2 by using a combination of nonisotopic in situ hybridization of chromosomal spreads and the polymerase chain reaction analysis of DNA samples isolated from somatic cell hybrids retaining deletions or translocations of chromosome 18.
Giordano S J; Yoo M; Ward D C; Bhatt M; Overhauser J; Steggles A W
Human genetics
1993
1993-12
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1007/bf00420948" target="_blank" rel="noreferrer noopener">10.1007/bf00420948</a>
THE HUMAN CYTOCHROME-B-5 GENE AND 4 OF ITS PSEUDOGENES ARE LOCATED ON CHROMOSOME 18Q23, 14Q31-32.1, 20P11.2, 14Q11.2-13 AND 14, RESPECTIVELY
Genetics & Heredity
Steggles A W; Giordano S J; Yoo M; Overhauser J; Bhatt M; Ward D
American Journal of Human Genetics
1993
1993-09
Journal Article
n/a
The isolation and characterization of the human cytochrome b5 gene.
Animals; Base Sequence; Cattle; Cell Line; Cloning; Cosmids; Cultured; Cytochromes b5/biosynthesis/*genetics; DNA Primers; DNA/genetics/isolation & purification; Gene Library; Genetic; Hominidae/*genetics; Humans; Introns; Luciferases/biosynthesis; Messenger/analysis/biosynthesis; Molecular; Molecular Sequence Data; Polymerase Chain Reaction; Promoter Regions; Rabbits; RNA; Transfection; Tumor Cells
From a series of lambda and cosmid libraries, we isolated DNA sequences corresponding to the complete coding region for the human cytochrome b5 (CYB5) gene. The overall gene organization was the same as the bovine and rabbit CYB5 genes. One cosmid clone containing exon I plus the 5' flanking region was extensively characterized. From this clone we obtained 2000 bp of 5' flanking sequence, containing several distinctive GC rich regions, potential trans-acting factor binding sites and a 74 bp direct repeat. A series of deletion constructs were made in the pGL2 luciferase vector and then used to transfect HepG2 and K562 cells. The data obtained suggest the presence of two promotors and one silencer region in the analyzed 5' sequence.
Li X R; Giordano S J; Yoo M; Steggles A W
Biochemical and biophysical research communications
1995
1995-04
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1006/bbrc.1995.1582" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1995.1582</a>
The Isolation And Characterization Of The Human Cytochrome-b5 Gene
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Li X R; Yoo M; Giordano S J; Steggles A W
Faseb Journal
1993
1993-04
Journal Article or Conference Abstract Publication
n/a