1
40
4
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1369/jhc.4A6455.2004" target="_blank" rel="noreferrer noopener">http://doi.org/10.1369/jhc.4A6455.2004</a>
Pages
1665–1674
Issue
12
Volume
52
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Characterization of vascular endothelial growth factor (VEGF) in the uterine cervix over pregnancy: effects of denervation and implications for cervical ripening.
Publisher
An entity responsible for making the resource available
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-12
Subject
The topic of the resource
Female; Animals; Immunohistochemistry; Pregnancy; Rats; Microcirculation; Phosphorylation; Down-Regulation; Enzyme-Linked Immunosorbent Assay; Reverse Transcriptase Polymerase Chain Reaction; Cervical Ripening/*metabolism; Cervix Uteri/blood supply/innervation/*metabolism; Denervation; Nitric Oxide Synthase Type III; Nitric Oxide Synthase/biosynthesis; Protein Isoforms/biosynthesis/metabolism; Protein-Serine-Threonine Kinases/biosynthesis; Proto-Oncogene Proteins c-akt; Proto-Oncogene Proteins/biosynthesis; Vascular Endothelial Growth Factor A/biosynthesis/*metabolism; Vascular Endothelial Growth Factor Receptor-2/biosynthesis; Wistar; Animal/*metabolism
Creator
An entity primarily responsible for making the resource
Mowa C N; Jesmin S; Sakuma I; Usip S; Togashi H; Yoshioka M; Hattori Y; Papka R
Description
An account of the resource
Bilateral neurectomy of the pelvic nerve (BLPN) that carries uterine cervix-related sensory nerves induces dystocia, and administration of its vasoactive neuropeptides induces changes in the cervical microvasculature, resembling those that occur in the ripening cervix. This study was designed to test the hypothesis that (a) the cervix of pregnant rats expresses vascular endothelial growth factor (VEGF) and components of the angiogenic signaling pathway [VEGF receptors (Flt-1, KDR), activity of protein kinase B, Akt (phosphorylated Akt), and endothelial nitric oxide synthase (eNOS)] and von Willebrand Factor (vWF) and that these molecules undergo changes with pregnancy, and (b) bilateral pelvic neurectomy (BLPN) alters levels of VEGF concentration in the cervix. Using RT-PCR and sequencing, two VEGF isoforms, 120 and 164, were identified in the rat cervix. VEGF, VEGF receptor-1 (Flt-1), eNOS, and vWF immunoreactivities (ir) were localized in the microvasculature of cervical stroma. Their protein levels increased during pregnancy but decreased to control levels by 2 days postpartum. VEGF receptor-2 (KDR)-ir was confined to the epithelium of the endocervix. BLPN downregulated levels of VEGF by a third. Therefore, the components of the angiogenic signaling pathway are expressed in the cervix and change over pregnancy. Furthermore, angiogenic and sensory neuronal factors may be important in regulating the dynamic microvasculature in the ripening cervix and may subsequently play a role in cervical ripening and the birth process.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1369/jhc.4A6455.2004" target="_blank" rel="noreferrer noopener">10.1369/jhc.4A6455.2004</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2004
Animal/*metabolism
Animals
Cervical Ripening/*metabolism
Cervix Uteri/blood supply/innervation/*metabolism
Denervation
Department of Anatomy & Neurobiology
Down-Regulation
Enzyme-Linked Immunosorbent Assay
Female
Hattori Y
Immunohistochemistry
Jesmin S
Microcirculation
Mowa C N
NEOMED College of Medicine
Nitric Oxide Synthase Type III
Nitric Oxide Synthase/biosynthesis
Papka R
Phosphorylation
Pregnancy
Protein Isoforms/biosynthesis/metabolism
Protein-Serine-Threonine Kinases/biosynthesis
Proto-Oncogene Proteins c-akt
Proto-Oncogene Proteins/biosynthesis
Rats
Reverse Transcriptase Polymerase Chain Reaction
Sakuma I
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Togashi H
Usip S
Vascular Endothelial Growth Factor A/biosynthesis/*metabolism
Vascular Endothelial Growth Factor Receptor-2/biosynthesis
Wistar
Yoshioka M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1677/jme.101548" target="_blank" rel="noreferrer noopener">http://doi.org/10.1677/jme.101548</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
343-359
Issue
2
Volume
33
Search for Full-text
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Aromatase Is Abundantly Expressed By Neonatal Rat Penis But Downregulated In Adulthood
Publisher
An entity responsible for making the resource available
Journal of Molecular Endocrinology
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-10
Subject
The topic of the resource
androgen receptor; beta messenger-rnas; brain; cytochrome-p450 aromatase; Endocrinology & Metabolism; estrogen-receptor-alpha; female reproductive organ; in-situ hybridization; leydig-cells; ribonucleic-acid; tract
Creator
An entity primarily responsible for making the resource
Jesmin S; Mowa C N; Sakuma I; Matsuda N; Togashi H; Yoshioka M; Hattori Y; Kitabatake A
Description
An account of the resource
Although synthesis of estrogen by male gonads has been well documented for over half a century, it is only recently that the role of estrogen in male reproductive events has gained appreciation. We recently reported abundant expression of estrogen receptor (ER)-alpha and -beta in different cell types of the rat penis, whose levels diminished with advancing age. The present study, which builds on data from the ER study, was designed to determine whether the penis is capable of generating its own local estrogen by examining evidence of the expression of aromatase, a microsomal enzymatic complex which irreversibly converts androgens to estrogens, using immunohistochemistry, Western blotting, in situ hybridization and real-time PCR analyses. Secondly, the effects of sex steroid hormones on penile aromatase were examined. Discrete aromatase immunoreactive cells were localized in primordial corpus cavernosum, corpus spongiosus and os penis, blood vessels and sensory corpuscle of glans penis. In situ hybridization signals corresponded with immunohistochemical findings. Western blot, enzyme immunoassay and real-time PCR analyses of rat penile samples revealed an age-dependent expression of aromatase and estrogen, with levels at week 1 almost resembling those of the ovary, but they decreased sharply by week 8, and decreased further by week 35. This expression pattern was strikingly similar to that of ER-alpha reported previously. Testosterone and diethylstilbesterol administered prenatally upregulate levels of aromatase mRNA and protein, and estrogen postnatally. Dihydrotestosterone upregulated aromatase mRNA and protein, but not estrogen. We conclude that estrogen acts via ER in a paracrine and/or autocrine manner to regulate penile events, particularly during development, and that estrogen synthesis is regulated by estrogen and androgens.
Identifier
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<a href="http://doi.org/10.1677/jme.101548" target="_blank" rel="noreferrer noopener">10.1677/jme.101548</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2004
androgen receptor
beta messenger-rnas
Brain
cytochrome-p450 aromatase
Endocrinology & Metabolism
estrogen-receptor-alpha
female reproductive organ
Hattori Y
in-situ hybridization
Jesmin S
Journal Article or Conference Abstract Publication
Journal of Molecular Endocrinology
Kitabatake A
leydig-cells
Matsuda N
Mowa C N
ribonucleic-acid
Sakuma I
Togashi H
tract
Yoshioka M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.brainres.2004.10.004" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.brainres.2004.10.004</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
172-182
Issue
2
Volume
1030
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
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Dublin Core
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Title
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Characterization Of Regional Cerebral Blood Flow And Expression Of Angiogenic Growth Factors In The Frontal Cortex Of Juvenile Male Shrsp And Shr
Publisher
An entity responsible for making the resource available
Brain Research
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-12
Subject
The topic of the resource
abnormalities; AD/HD; angiogemic factor; animal-model; attention-deficit/hyperactivity; brain; children; deficit-hyperactivity-disorder; disorder; frontal cortex; Neurosciences & Neurology; nitric-oxide; NOS isoform; regional cerebral blood flow; spontaneously hypertensive-rats; stroke-prone; vegf
Creator
An entity primarily responsible for making the resource
Jesmin S; Togashi H; Mowa C N; Ueno K; Yamaguchi T; Shibayama A; Miyauchi T; Sakuma I; Yoshioka M
Description
An account of the resource
Attention-deficit/hyperactivity disorder (AD/HD) is a common pediatric behavioral disorder associated with male preponderance and reduction of regional cerebral blood flow (rCBF). However, lack of an appropriate animal model exhibiting appropriate AD/HD symptoms stands in the way of studying mechanism(s) underlying reduced rCBF and male preponderance. Our group has been investigating the suitability of juvenile male stroke-prone spontaneously hypertensive rats (SHRSP), a substrain of the commonly used AD/HD animal model SHR, as a model for AD/HD because, unlike SHR, SHRSP displays cognitive impairment and male preponderance. Our more recent studies revealed alterations in the synthesis of sex steroid hormones and angiogenic factors in the frontal cortex of male SHRSP compared to the genetic control WKY. Based on these observations, the present study utilizes laser-Doppler flowmetry, histochemistry, enzyme immunoassay, immunoblotting, and real-time PCR to characterize and compare the patterns of regional cerebral blood flow and synthesis of angiogenic molecules [basic fibroblast growth factor; nitric oxide synthase isoforms (endothelial, neuronal and inducible); vascular endothelial growth factor (VEGF) and its signaling molecules VEGF receptors, phosphorylated Akt, endothelial nitric oxide synthase eNOS] between male SHRSP and SHR. Overall, consistent with our previous data showing alteration in VEGF/Akt/NO signaling, there was a marked reduction in the profile of rCBF (35%) and angiogenic factors of SHRSP, compared to age-matched genetic control Wistar-Kyoto rats (WKY) and SHR. We conclude that, unlike SHR, the profiles of rCBF and angiogenic factors in SHRSP are altered in juvenile male. Thus, SHRSP appears to be a more suitable animal model for studying changes in rCBF in AD/HD. (C) 2004 Elsevier B.V. All rights reserved.
Identifier
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<a href="http://doi.org/10.1016/j.brainres.2004.10.004" target="_blank" rel="noreferrer noopener">10.1016/j.brainres.2004.10.004</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2004
Abnormalities
AD/HD
angiogemic factor
animal-model
attention-deficit/hyperactivity
Brain
Brain research
Children
deficit-hyperactivity-disorder
disorder
frontal cortex
Jesmin S
Journal Article or Conference Abstract Publication
Miyauchi T
Mowa C N
Neurosciences & Neurology
nitric-oxide
NOS isoform
regional cerebral blood flow
Sakuma I
Shibayama A
spontaneously hypertensive-rats
stroke-prone
Togashi H
Ueno K
vegf
Yamaguchi T
Yoshioka M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1210/en.2004-0487" target="_blank" rel="noreferrer noopener">http://doi.org/10.1210/en.2004-0487</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
4330-4343
Issue
9
Volume
145
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Gonadal Hormones And Frontocortical Expression Of Vascular Endothelial Growth Factor In Male Stroke-prone, Spontaneously Hypertensive Rats, A Model For Attention-deficit/hyperactivity Disorder
Publisher
An entity responsible for making the resource available
Endocrinology
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-09
Subject
The topic of the resource
aged female rats; animal-model; central-nervous-system; cerebral-blood-flow; computed tomography; deficit-hyperactivity-disorder; emission; Endocrinology & Metabolism; estrogen-receptor-alpha; in-situ hybridization; insulin-resistant; messenger-ribonucleic-acid; stage
Creator
An entity primarily responsible for making the resource
Jesmin S; Togashi H; Sakuma I; Mowa C N; Ueno K I; Yamaguchi T; Yoshioka M; Kitabatake A
Description
An account of the resource
Attention-deficit/hyperactivity disorder (AD/HD) is a common pediatric behavioral disorder associated, in part, with male preponderance and reduced regional cerebral blood flow (rCBF). However, mechanism(s) underlying male preponderance and reduced rCBF in AD/HD are unclear. The present study profiles the expression of angiogenic and hormonal factors likely to underlie these symptoms using a recently characterized AD/HD animal model, juvenile male stroke-prone spontaneously hypertensive rats (SHRSP). Because vascular endothelial growth factor (VEGF) signaling cascade and gonadal steroids are key regulators of angiogenesis and gender-based behavior, respectively, we profiled their patterns of expression in the frontal cortex of SHRSP to elucidate their roles in the genesis of AD/HD male preponderance and rCBF. Interestingly, levels of VEGF, VEGF receptors (KDR, Flt-1), endothelial nitric oxide synthase, phosphorylated. Akt (pAkt), estrogen receptor-alpha, aromatase, and capillary density in sham-operated SHRSP were remarkably down-regulated, whereas androgen receptor levels were up-regulated, compared with age-matched genetic control, Wistar-Kyoto rats. Castration, estrogen, and androgen receptor antagonist (flutamide) counteracted these effects. Dihydrotestosterone, but not testosterone, reversed the beneficiary effects of castration. Estrogen receptor-beta levels remained unchanged in all groups examined. We postulate that changes in androgen metabolism that tend to up-regulate local dihydrotestosterone concentration and diminish estrogen synthesis, in the frontal cortex of juvenile male SHRSP, may lower levels and/or activity of VEGF and its signaling cascade and, subsequently, reduce rCBF. These findings could, in part, help explain the pathogenesis of reduced rCBF and male preponderance in AD/HD.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1210/en.2004-0487" target="_blank" rel="noreferrer noopener">10.1210/en.2004-0487</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2004
aged female rats
animal-model
central-nervous-system
cerebral-blood-flow
computed tomography
deficit-hyperactivity-disorder
emission
Endocrinology
Endocrinology & Metabolism
estrogen-receptor-alpha
in-situ hybridization
insulin-resistant
Jesmin S
Journal Article or Conference Abstract Publication
Kitabatake A
messenger-ribonucleic-acid
Mowa C N
Sakuma I
stage
Togashi H
Ueno K I
Yamaguchi T
Yoshioka M