1
40
8
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
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n/a
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A1342-A1343
Issue
5
Volume
17
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Suppression Of The Human Sterol 12 Alpha Hydroxylase (cyp8b1) Gene By Interleukin-1 Beta (il-1 Beta)
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-03
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Jahan A; Zhang M; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2003
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Faseb Journal
Jahan A
Journal Article or Conference Abstract Publication
Life Sciences & Biomedicine - Other
Topics
Zhang M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Volume
129
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Molecular mechanisms of nuclear receptor regulation of bile acid synthesis
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003
Subject
The topic of the resource
transactivation; feedback-regulation; identification; cholesterol 7-alpha-hydroxylase; transcription; heterodimer partner; repression; shp; factor 4-alpha; 7-alpha-hydroxylase gene cyp7a1
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Zhang M; Chen W; Norlin M; Owsley E
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Book/Monograph
2003
7-alpha-hydroxylase gene cyp7a1
Book/Monograph
Chen W
Chiang J Y L
cholesterol 7-alpha-hydroxylase
factor 4-alpha
feedback-regulation
heterodimer partner
identification
Norlin M
Owsley E
repression
SHP
transactivation
Transcription
Zhang M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A562-A563
Issue
4
Volume
16
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Title
A name given to the resource
On the mechanism of bile acid inhibition of rat sterol 12 alpha -hydroxylase gene (CYP8B1) transcription by nuclear receptors, alpha -fetoprotein transcription factor (FTF) and hepatocyte nuclear receptor 4 alpha (HNF4 alpha )
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-03
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Yang Y; Zhang M; Eggertsen G
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2002
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Eggertsen G
Faseb Journal
Journal Article or Conference Abstract Publication
Life Sciences & Biomedicine - Other
Topics
Yang Y
Zhang M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Volume
120
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Nuclear receptor regulation of the human cholesterol 7 alpha-hydroxylase, sterol 27-hydroxylase and sterol 12 alpha-hydroxylase genes in bile acid synthesis
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001
Subject
The topic of the resource
rat; biosynthesis; transcription factor; expression; hepatocytes; messenger-rna; pathway; activation; identification; cyp7a
Creator
An entity primarily responsible for making the resource
Chiang J Y L; Chen W; Zhang M; Cowsley E; Yang Y Z
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Book/Monograph
2001
activation
biosynthesis
Book/Monograph
Chen W
Chiang J Y L
Cowsley E
cyp7a
expression
hepatocytes
identification
messenger-rna
pathway
rat
transcription factor
Yang Y Z
Zhang M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A182-A182
Issue
4
Volume
15
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional regulation of human 12 alpha-hydroxylase gene (CYP8B1) by bile acids and nuclear receptors
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-03
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Zhang M; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2001
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Faseb Journal
Journal Article
Life Sciences & Biomedicine - Other
Topics
Zhang M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A1431-A1431
Issue
8
Volume
14
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Title
A name given to the resource
Characterization of human 12 alpha-hydroxylase gene (CYP8B1) promoter and regulation by bile acids
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-05
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
An entity primarily responsible for making the resource
Zhang M; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2000
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Faseb Journal
Journal Article
Life Sciences & Biomedicine - Other
Topics
Zhang M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s1388-1981(02)00186-5" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s1388-1981(02)00186-5</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
63-73
Issue
1
Volume
1583
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Title
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On the mechanism of bile acid inhibition of rat sterol 12 alpha-hydroxylase gene (CYP8B1) transcription: roles of alpha-fetoprotein transcription factor and hepatocyte nuclear factor 4 alpha
Publisher
An entity responsible for making the resource available
Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-06
Subject
The topic of the resource
activation; bile acid synthesise; Biochemistry & Molecular Biology; Biophysics; biosynthesis; Cell Biology; cholesterol 7-alpha-hydroxylase gene; Cyp7a1; cytochrome P450; expression; feedback-regulation; gene regulation; liver microsomal metabolism; nuclear receptor; promoter; receptor; repression
Creator
An entity primarily responsible for making the resource
Yang Y Z; Zhang M; Eggertsen G; Chiang J Y L
Description
An account of the resource
The sterol 12alpha-hydroxylase (CYP8B1) is a key enzyme of the bile acid biosynthetic pathway. It regulates the composition of bile acids in bile, i.e. ratio between cholic acid (CA) and chenodeoxycholic acid (CDCA). In similarity with cholesterol 7alpha-hydroxylase (CYP7A1), this enzyme is subjected to a negative feedback regulation by bile acids, It has been recently reported that bile acid-activated famesoid X receptor (FXR) induces the small heterodimer partner (SHP) that interacts with alpha-fetoprotein transcription factor (FTF) and down-regulates CYP7A1 transcription. We studied whether the same mechanism also regulated rat CYP8B1 gene transcription. Feeding rats with CDCA caused a 40-50% decrease of CYP8B1 and hepatocyte nuclear factor 4alpha (HNF4alpha) mRNA expression levels. This was associated with an increase in FTF mRNA expression, but SHP mRNA expression was not altered. Electrophoretic mobility shift assay (EMSA) and transient transfection assay of promoter/reporter genes coupled to mutagenesis analysis identified a putative bile acid response element (BARE) that has an HNF4alpha binding site embedded in two overlapping FTF binding sites. Mutation of the HNF4alpha binding site markedly reduced basal promoter activity and its repression by bile acids. Cotransfection with FTF strongly repressed CYP8B1 transcription. Interestingly, HNF4alpha could overcome the inhibitory effects of FTF and bile acids. We conclude that FTF and HNF4alpha not only play critical roles on CYP8B1 gene transcription, but also mediate bile acid feedback inhibition. This study reveals a novel mechanism by which bile acids inhibit rat CYP8B1 gene transcription by inducing FTF and inhibiting HNF4alpha expression. (C) 2002 Elsevier Science B.V. All rights reserved.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s1388-1981(02)00186-5" target="_blank" rel="noreferrer noopener">10.1016/s1388-1981(02)00186-5</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2002
activation
bile acid synthesise
Biochemistry & Molecular Biology
Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids
Biophysics
biosynthesis
Cell Biology
Chiang J Y L
cholesterol 7-alpha-hydroxylase gene
CYP7A1
cytochrome P450
Eggertsen G
expression
feedback-regulation
Gene Regulation
Journal Article
liver microsomal metabolism
Nuclear Receptor
promoter
Receptor
repression
Yang Y Z
Zhang M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M105117200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M105117200</a>
Pages
41690–41699
Issue
45
Volume
276
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional regulation of the human sterol 12alpha-hydroxylase gene (CYP8B1): roles of heaptocyte nuclear factor 4alpha in mediating bile acid repression.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-11
Subject
The topic of the resource
*DNA-Binding Proteins; *Transcription; Base Sequence; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Bile Acids and Salts/*pharmacology; Binding Sites; Cholesterol 7-alpha-Hydroxylase/genetics; Cultured; Cytochrome P-450 Enzyme System/*genetics; Cytoplasmic and Nuclear/physiology; Genetic; Genetic/physiology; Hepatocyte Nuclear Factor 4; Humans; Molecular Sequence Data; Phosphoproteins/*physiology; Promoter Regions; Receptors; Repressor Proteins/*pharmacology; Steroid 12-alpha-Hydroxylase; Steroid Hydroxylases/*genetics; Transcription Factors/*physiology; Tumor Cells
Creator
An entity primarily responsible for making the resource
Zhang M; Chiang J Y
Description
An account of the resource
Sterol 12alpha-hydroxylase catalyzes the synthesis of cholic acid and controls the ratio of cholic acid over chenodeoxycholic acid in the bile. Transcription of CYP8B1 is inhibited by bile acids, cholesterol, and insulin. To study the mechanism of CYP8B1 transcription by bile acids, we have cloned and determined 3389 base pairs of the 5'-upstream nucleotide sequences of the human CYP8B1. Deletion analysis of CYP8B1/luciferase reporter activity in HepG2 cells revealed that the sequences from -57 to +300 were important for basal and liver-specific promoter activities. Hepatocyte nuclear factor 4alpha (HNF4alpha) strongly activated human CYP8B1 promoter activities, whereas cholesterol 7alpha-hydroxylase promoter factor (CPF), an NR5A2 family of nuclear receptors, had much less effect. Electrophoretic mobility shift assay identified an overlapping HNF4alpha- and CPF-binding site in the +198/+227 region. The human CYP8B1 promoter activities were strongly repressed by bile acids, and the bile acid response element was localized between +137 and +220. Site-directed mutagenesis of the HNF4alpha-binding site markedly reduced promoter activity and its response to bile acid repression. On the other hand, mutation of the
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M105117200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M105117200</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*DNA-Binding Proteins
*Transcription
2001
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Bile Acids and Salts/*pharmacology
Binding Sites
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/genetics
Cultured
Cytochrome P-450 Enzyme System/*genetics
Cytoplasmic and Nuclear/physiology
Department of Integrative Medical Sciences
Genetic
Genetic/physiology
Hepatocyte Nuclear Factor 4
Humans
Molecular Sequence Data
NEOMED College of Medicine
Phosphoproteins/*physiology
Promoter Regions
Receptors
Repressor Proteins/*pharmacology
Steroid 12-alpha-Hydroxylase
Steroid Hydroxylases/*genetics
The Journal of biological chemistry
Transcription Factors/*physiology
Tumor Cells
Zhang M