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40
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.jneumeth.2017.08.029" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.jneumeth.2017.08.029</a>
Pages
227–237
Volume
291
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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An operant-based detection method for inferring tinnitus in mice.
Publisher
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Journal of neuroscience methods
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-11
Subject
The topic of the resource
*Conditioning; *Disease Models; *Inferior colliculus; *Mouse model; *Noise-induced hearing loss; *Operant conditioning; *Sodium salicylate; *Tinnitus; Acoustic Stimulation; Analysis of Variance; Animal; Animals; Auditory; Avoidance Learning; Brain Stem/physiology; Electroshock; Equipment Design; Evoked Potentials; Female; Inbred C57BL; Inferior Colliculi/physiopathology; Male; Mice; Motor Activity; Neurons/physiology; Operant; Otoacoustic Emissions; Sodium Salicylate; Spontaneous/physiology; Tinnitus/*diagnosis/physiopathology; Tissue Culture Techniques; Voltage-Sensitive Dye Imaging
Creator
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Zuo Hongyan; Lei Debin; Sivaramakrishnan Shobhana; Howie Benjamin; Mulvany Jessica; Bao Jianxin
Description
An account of the resource
BACKGROUND: Subjective tinnitus is a hearing disorder in which a person perceives sound when no external sound is present. It can be acute or chronic. Because our current understanding of its pathology is incomplete, no effective cures have yet been established. Mouse models are useful for studying the pathophysiology of tinnitus as well as for developing therapeutic treatments. NEW METHOD: We have developed a new method for determining acute and chronic tinnitus in mice, called sound-based avoidance detection (SBAD). The SBAD method utilizes one paradigm to detect tinnitus and another paradigm to monitor possible confounding factors, such as motor impairment, loss of motivation, and deficits in learning and memory. RESULTS: The SBAD method has succeeded in monitoring both acute and chronic tinnitus in mice. Its detection ability is further validated by functional studies demonstrating an abnormal increase in neuronal activity in the inferior colliculus of mice that had previously been identified as having tinnitus by the SBAD method. COMPARISON WITH EXISTING METHODS: The SBAD method provides a new means by which investigators can detect tinnitus in a single mouse accurately and with more control over potential confounding factors than existing methods. CONCLUSION: This work establishes a new behavioral method for detecting tinnitus in mice. The detection outcome is consistent with functional validation. One key advantage of mouse models is they provide researchers the opportunity to utilize an extensive array of genetic tools. This new method could lead to a deeper understanding of the molecular pathways underlying tinnitus pathology.
Identifier
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<a href="http://doi.org/10.1016/j.jneumeth.2017.08.029" target="_blank" rel="noreferrer noopener">10.1016/j.jneumeth.2017.08.029</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Conditioning
*Disease Models
*Inferior colliculus
*Mouse model
*Noise-induced hearing loss
*Operant conditioning
*Sodium salicylate
*Tinnitus
2017
Acoustic Stimulation
Analysis of Variance
Animal
Animals
Auditory
Avoidance Learning
Bao Jianxin
Brain Stem/physiology
Department of Anatomy & Neurobiology
Electroshock
Equipment Design
Evoked Potentials
Female
Howie Benjamin
Inbred C57BL
Inferior Colliculi/physiopathology
Journal of neuroscience methods
Lei Debin
Male
Mice
Motor Activity
Mulvany Jessica
NEOMED College of Medicine
Neurons/physiology
Operant
Otoacoustic Emissions
Sivaramakrishnan Shobhana
Sodium Salicylate
Spontaneous/physiology
Tinnitus/*diagnosis/physiopathology
Tissue Culture Techniques
Voltage-Sensitive Dye Imaging
Zuo Hongyan
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.heares.2018.07.008" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.heares.2018.07.008</a>
Pages
88–96
Volume
367
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Detection of single mRNAs in individual cells of the auditory system.
Publisher
An entity responsible for making the resource available
Hearing research
Date
A point or period of time associated with an event in the lifecycle of the resource
2018
2018-09
Subject
The topic of the resource
Cochlea; Immunohistochemistry; Inner hair cell; Outer hair cell; Single-molecule fluorescence in situ hybridization; Spiral ganglion neuron
Creator
An entity primarily responsible for making the resource
Salehi Pezhman; Nelson Charlie N; Chen Yingying; Lei Debin; Crish Samuel D; Nelson Jovitha; Zuo Hongyan; Bao Jianxin
Description
An account of the resource
Gene expression analysis is essential for understanding the rich repertoire of cellular functions. With the development of sensitive molecular tools such as single-cell RNA sequencing, extensive gene expression data can be obtained and analyzed from various tissues. Single-molecule fluorescence in situ hybridization (smFISH) has emerged as a powerful complementary tool for single-cell genomics studies because of its ability to map and quantify the spatial distributions of single mRNAs at the subcellular level in their native tissue. Here, we present a detailed method to study the copy numbers and spatial localizations of single mRNAs in the cochlea and inferior colliculus. First, we demonstrate that smFISH can be performed successfully in adult cochlear tissue after decalcification. Second, we show that the smFISH signals can be detected with high specificity. Third, we adapt an automated transcript analysis pipeline to quantify and identify single mRNAs in a cell-specific manner. Lastly, we show that our method can be used to study possible correlations between transcriptional and translational activities of single genes. Thus, we have developed a detailed smFISH protocol that can be used to study the expression of single mRNAs in specific cell types of the peripheral and central auditory systems.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.heares.2018.07.008" target="_blank" rel="noreferrer noopener">10.1016/j.heares.2018.07.008</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2018
Bao Jianxin
Chen Yingying
Cochlea
Crish Samuel D
Department of Anatomy & Neurobiology
Department of Pharmaceutical Sciences
Hearing research
Immunohistochemistry
Inner hair cell
Lei Debin
Nelson Charlie N
Nelson Jovitha
NEOMED College of Medicine
NEOMED College of Pharmacy
Outer hair cell
Salehi Pezhman
Single-molecule fluorescence in situ hybridization
Spiral ganglion neuron
Zuo Hongyan