Serotonin-activated alpha 2-macroglobulin inhibits neurite outgrowth and survival of embryonic sensory and cerebral cortical neurons.
Afferent/drug effects/ultrastructure; alpha-Macroglobulins/metabolism/*pharmacology; Animals; Bovine/pharmacology; Cell Survival/drug effects; Cells; Chick Embryo; Cultured; Frontal Lobe/*cytology/embryology; Ganglia; Humans; Nerve Growth Factors/*antagonists & inhibitors/pharmacology; Neurites/drug effects/ultrastructure; Neurons; Neurons/*drug effects/ultrastructure; Protein Binding; Rats; Serotonin/metabolism/*pharmacology; Serum Albumin; Spinal/*cytology/embryology
Methylamine-modified alpha-2-macroglobulin (MA-alpha 2M) has been recently shown to inhibit the biological activity of beta-nerve growth factor (NGF) in promoting neurite outgrowth by embryonic dorsal root ganglia in culture (Koo PH, Liebl DJ, J Neurosci Res 31:678-692, 1992). The objectives of this study are to determine whether alpha 2M can also be modified by larger aromatic biogenic amines such as
Liebl D J; Koo P H
Journal of neuroscience research
1993
1993-06
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1002/jnr.490350207" target="_blank" rel="noreferrer noopener">10.1002/jnr.490350207</a>
Inhibition of nerve growth factor-stimulated neurite outgrowth by methylamine-modified alpha 2-macroglobulin.
alpha-Macroglobulins/metabolism/*pharmacology; Animals; Cells; Chick Embryo; Cultured; Drug Interactions; Female; Ganglia; Humans; Male; Methylamines/*pharmacology; Mice; Nerve Growth Factors/antagonists & inhibitors/metabolism/*pharmacology; Neurites/drug effects/*physiology/ultrastructure; Neurons/cytology/physiology; Placenta; Pregnancy; Spinal/physiology; Submandibular Gland
alpha 2-Macroglobulin (alpha 2M) is a rather ubiquitous protein in extracellular spaces of mammals. It is an inhibitor of endopeptidases, can be modified by aliphatic amines, and combines with a number of hormones/cytokines such as beta-nerve growth factor (NGF) [Koo PH, Stach RW (1989): J Neurosci Res 22:247]. The objective of this study is to compare the NGF-binding properties of methylamine-modified human alpha 2M (MA-alpha 2M) versus normal alpha 2M and their effects on the biological activity of NGF and neurite extension by embryonic chicken dorsal root ganglia. As determined by gel filtration, polyacrylamide gel electrophoresis, and equilibrium binding studies, these two forms of alpha 2M are similar in their binding affinities, with MA-alpha 2M binding about twice as much NGF as normal alpha 2M. Both normal alpha 2M and
Koo P H; Liebl D J
Journal of neuroscience research
1992
1992-04
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1002/jnr.490310412" target="_blank" rel="noreferrer noopener">10.1002/jnr.490310412</a>