1
40
4
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<table width="91" style="border-collapse:collapse;width:68pt;"><colgroup><col width="91" style="width:68pt;" /></colgroup><tbody><tr style="height:15pt;"><td width="91" height="20" class="xl18" style="width:68pt;height:15pt;"><a href="http://doi.org/10.1080/15548627.2020.1797280">http://doi.org/10.1080/15548627.2020.1797280</a></td>
</tr></tbody></table>
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Update Year & Number
Jan to Aug list 2021
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition).
Creator
An entity primarily responsible for making the resource
Multiple
Publisher
An entity responsible for making the resource available
Autophagy
Date
A point or period of time associated with an event in the lifecycle of the resource
2021
Description
An account of the resource
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
Identifier
An unambiguous reference to the resource within a given context
<table width="91" style="border-collapse:collapse;width:68pt;"><colgroup><col width="91" style="width:68pt;" /></colgroup><tbody><tr style="height:15pt;"><td width="91" height="20" class="xl18" style="width:68pt;height:15pt;"><a href="http://doi.org/10.1080/15548627.2020.1797280">http://doi.org/10.1080/15548627.2020.1797280</a></td>
</tr></tbody></table>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2021
autophagosome
Cancer
Flux
LC3
lysosome
macroautophagy
Neurodegeneration
phagophore
Stress
vacuole
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1080/15548627.2020.1797280" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/15548627.2020.1797280</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1-382
ISSN
1554-8635 1554-8627
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<a href="http://neomed.idm.oclc.org/login?url=http://doi.org/10.1080/15548627.2020.1797280" target="_blank" rel="noreferrer noopener">NEOMED Full-text Holding (if available) - Proxy DOI: 10.1080/15548627.2020.1797280</a>
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Update Year & Number
March 2021 List
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition).
Publisher
An entity responsible for making the resource available
Autophagy
Date
A point or period of time associated with an event in the lifecycle of the resource
2021
2021-02-08
Subject
The topic of the resource
Autophagosome; cancer; flux; LC3; lysosome; macroautophagy; neurodegeneration; phagophore; stress; vacuole
Description
An account of the resource
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1080/15548627.2020.1797280" target="_blank" rel="noreferrer noopener">10.1080/15548627.2020.1797280</a>
Format
The file format, physical medium, or dimensions of the resource
journalArticle
2021
autophagosome
Autophagy
Cancer
Department of Anatomy & Neurobiology
Flux
journalArticle
LC3
lysosome
macroautophagy
March 2021 List
NEOMED College of Medicine
Neurodegeneration
phagophore
Stress
vacuole
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jcp.27071" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jcp.27071</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Autophagy plays an essential role in bone homeostasis.
Publisher
An entity responsible for making the resource available
Journal of cellular physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2019
2019-02
Subject
The topic of the resource
osteoclast; autophagosome; autophagy; osteoblast; osteocytes
Creator
An entity primarily responsible for making the resource
Jaber Fatima A; Khan Nazir M; Ansari Mohammad Y; Al-Adlaan Asaad A; Hussein Nazar J; Safadi Fayez F
Description
An account of the resource
Autophagy is very critical for multiple cellular processes. Autophagy plays a critical role in bone cell differentiation and function.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/jcp.27071" target="_blank" rel="noreferrer noopener">10.1002/jcp.27071</a>
2019
Al-Adlaan Asaad A
Ansari Mohammad Y
autophagosome
Autophagy
Department of Anatomy & Neurobiology
Hussein Nazar J
Jaber Fatima A
Journal of cellular physiology
Khan Nazir M
NEOMED College of Medicine
OSTEOBLAST
osteoclast
osteocytes
Safadi Fayez F
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1167/iovs.14-16126" target="_blank" rel="noreferrer noopener">http://doi.org/10.1167/iovs.14-16126</a>
Pages
1437–1446
Issue
3
Volume
56
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Mitochondrial morphology differences and mitophagy deficit in murine glaucomatous optic nerve.
Publisher
An entity responsible for making the resource available
Investigative ophthalmology & visual science
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015-02
Subject
The topic of the resource
*Disease Models; Adenosine Triphosphate/metabolism; Age Factors; Animal; Animals; autophagosome; Axons/pathology; Electron; Inbred DBA; Mice; Microscopy; mitochondria; Mitochondria/*pathology; Mitochondrial Degradation/*physiology; mitophagy; Myelinated/pathology; Nerve Fibers; Optic Nerve/*pathology
Creator
An entity primarily responsible for making the resource
Coughlin Lucy; Morrison Richard S; Horner Philip J; Inman Denise M
Description
An account of the resource
PURPOSE: Decreased ATP correlates with intraocular pressure exposure in the optic nerves of mice with glaucoma. To understand what underlies this energy deficit, we examined mitochondria in the myelinated optic nerve axons of the DBA/2J mouse, a model of glaucoma secondary to iris pigment disease, and the DBA/2(wt-gpnmb) control strain. METHODS: Mitochondrial length, width, surface area, and health status were measured in 30 electron microscopic fields within the myelinated portion of optic nerves from DBA/2J and DBA/2(wt-gpnmb) mice at 3, 6, and 10 months of age. Protein was isolated from optic nerve for analysis of PINK1, Parkin, LC3-I and -II, and lysosome-associated membrane protein 1 (LAMP1) by Western blot. RESULTS: The number of mitochondria in DBA/2J optic nerve was increased, and they had significantly smaller surface area. Mitochondria in DBA/2J were closer to the axolemma, more spatially isolated, and their cristae were more disrupted at every age group as compared to DBA/2(wt-gpnmb). Autophagosomes were significantly increased in DBA/2J optic nerve at all ages. Protein analysis showed higher LC3-II to LC3-I ratio in aged DBA/2J optic nerve than in DBA/2(wt-gpnmb). PINK1 and Parkin levels were not statistically different across age groups. LAMP1 was significantly decreased in the aged DBA/2J optic nerve. CONCLUSIONS: Decreased surface area, combined with reduced oxidative capacity in mitochondria from the aged DBA/2J axon, indicate that mitochondrial pathology may contribute to the energy deficit in glaucomatous optic nerve. Though autophagosomes were increased in DBA/2J optic nerve, the increased mitochondria and decreased LAMP1 suggest deteriorating mitochondria are not being efficiently recycled by mitophagy.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1167/iovs.14-16126" target="_blank" rel="noreferrer noopener">10.1167/iovs.14-16126</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Disease Models
2015
Adenosine Triphosphate/metabolism
Age Factors
Animal
Animals
autophagosome
Axons/pathology
Coughlin Lucy
Department of Pharmaceutical Sciences
Electron
Horner Philip J
Inbred DBA
Inman Denise M
Investigative ophthalmology & visual science
Mice
Microscopy
Mitochondria
Mitochondria/*pathology
Mitochondrial Degradation/*physiology
mitophagy
Morrison Richard S
Myelinated/pathology
NEOMED College of Pharmacy
Nerve Fibers
Optic Nerve/*pathology