1
40
1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1023/a:1023936503105" target="_blank" rel="noreferrer noopener">http://doi.org/10.1023/a:1023936503105</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
147-154
Issue
3
Volume
39
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Experiments With Osteoblasts Cultured Under Varying Orientations With Respect To The Gravity Vector
Publisher
An entity responsible for making the resource available
Cytotechnology
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002
Subject
The topic of the resource
attachment; averaged gravity; Biotechnology & Applied Microbiology; bone; Cell Biology; cell-culture; clinostat; gene-expression; growth; growth rate; in-vitro; inversion; microgravity; osteoblasts; proliferation; space; substrate
Creator
An entity primarily responsible for making the resource
Kacena M A; Todd P; Gerstenfeld L C; Landis W J
Description
An account of the resource
Substrate attachment is crucial for normal growth and differentiation of many cell types. To better understand the role of gravity in osteoblast attachment and growth in vitro, 17-day-old embryonic chick calvarial osteoblasts were subjected to directional variations with respect to gravity. Osteoblasts, grown in MEM or DME supplemented with 10% FBS and attached to type I collagen-coated coverslips, were loaded into cylindrical containers completely filled with medium and oriented so that cells were either atop or beneath, or coverslips continuously rotated (similar to2 rpm) in a clinostat, thereby continuously changing their orientation with respect to gravity. Cells in these three conditions were collected daily for up to 6 days, and cell viability, two osteoblast functions, and proliferation were assessed. Data suggest the number and function of attached osteoblasts is unaltered by inversion or clino-rotation in initially confluent cultures. In sparsely plated cultures, however, osteoblast viability was significantly decreased (similar to50%) in inverted and rotated cultures during the first 3 days of sampling, but from days 4 - 6 no significant difference was found in viable cell number for the three conditions. Decreases in viable cell number within the first days of the experiments could result from death followed by detachment, detachment followed by death, differences in proliferation rate, or lag-phase duration. To help distinguish among these, BrdU labeling for 2 or 24 hr was used to assess cell proliferation rate. Log-phase growth rates were calculated and were unchanged among the three conditions tested. These results point to an increase in lag-phase duration in inverted and rotated cultures. In summary, changing the cell-substrate attachment direction with respect to gravity causes an immediate response in the form of diminished viable osteoblast number in sparse, early cultures, but the effect disappears after 3 - 4 days and does not occur in mature, confluent cultures.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1023/a:1023936503105" target="_blank" rel="noreferrer noopener">10.1023/a:1023936503105</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2002
attachment
averaged gravity
Biotechnology & Applied Microbiology
Bone
Cell Biology
cell-culture
clinostat
Cytotechnology
gene-expression
Gerstenfeld L C
growth
growth rate
in-vitro
inversion
Journal Article or Conference Abstract Publication
Kacena M A
Landis W J
microgravity
Osteoblasts
proliferation
space
substrate
Todd P