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Text
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<a href="http://doi.org/10.1016/0167-0115(91)90061-k" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0167-0115(91)90061-k</a>
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Pages
263-270
Issue
2
Volume
36
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Title
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ALTERATION OF SOMATOSTATIN BUT NOT GROWTH HORMONE-RELEASING FACTOR PITUITARY BINDING-SITES IN OBESE ZUCKER RATS
Publisher
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Regulatory Peptides
Date
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1991
1991-10
Subject
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binding site; cells; defect; Endocrinology & Metabolism; growth hormone-releasing factor; hypothalamus; messenger-rna; Physiology; pituitary; secretion; somatostatin; tissue; zucker rat
Creator
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Abribat T; Finkelstein J A; Gaudreau P
Description
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The present study was designed to determine whether the diminution of growth hormone (GH) secretion that occurs in obese Zucker rats is related to alterations of GH-releasing factor (GRF) or somatostatin (SRIF) pituitary binding sites. Cold saturation studies were performed in pituitary homogenates of 4-month-old lean and obese rats, using [I-125-Tyr-10]hGRF(1-44)NH-2 as radioligand and [I-127-Tyr-10]hGRF-(1-44)NH-2 as competitor, and in pituitary membrane preparations, using [I-125-Tyr-0, D-Trp-8]SRIF14 as radioligand and [I-127-Tyr-0, D-Trp-8]SRIF14 as competitor. In lean rats, analysis of the curves by the Ligand program revealed the presence of two distinct classes of GRF binding sites, the first being of high affinity (0.74 +/- 0.11 nM) and low capacity (118 +/- 31 fmol/mg protein), the second being of lower affinity (880 +/- 240 nM) and higher capacity (140 +/- 35 pmol/mg protein), and of a single class of SRIF binding sites (affinity: 0.40 +/- 0.12 nM; capacity: 24 +/- 6 fmol/mg protein). In obese rats, no difference was observed in GRF binding parameters for both classes of sites, but the concentration of somatostatin binding sites was reduced by 67% when compared to their lean littermates. These findings suggest that the SRIF pituitary receptors are down-regulated in obese Zucker rats and indicate that no alteration of GRF pituitary binding sites contribute to the blunted GH secretion observed in this model of obesity.
Identifier
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<a href="http://doi.org/10.1016/0167-0115(91)90061-k" target="_blank" rel="noreferrer noopener">10.1016/0167-0115(91)90061-k</a>
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Journal Article or Conference Abstract Publication
1991
Abribat T
binding site
Cells
defect
Endocrinology & Metabolism
Finkelstein J A
Gaudreau P
growth hormone-releasing factor
Hypothalamus
Journal Article or Conference Abstract Publication
messenger-rna
Physiology
pituitary
Regulatory Peptides
secretion
somatostatin
tissue
zucker rat