Decompression syndrome and the evolution of deep diving physiology in the Cetacea
Cetacea; ocean; behavior; Science & Technology - Other Topics; blue; avascular osteonecrosis; beaked-whales; behaviorally induced paleopathology; dive; diving; gas-bubble lesions; mysticeti; odontoceti; Physiology; ziphiidae
Whales repetitively dive deep to feed and should be susceptible to decompression syndrome, though they are not known to suffer the associated pathologies. Avascular osteonecrosis has been recognized as an indicator of diving habits of extinct marine amniotes. Vertebrae of 331 individual modern and 996 fossil whales were subjected to macroscopic and radiographic examination. Avascular osteonecrosis was found in the Oligocene basal odontocetes (Xenorophoidea) and in geologically younger mysticetes, such as Aglaocetus [a sister taxon to Balaenopteridae+(Balaenidae+Eschrichtiidae) clade]. These are considered as early "experiments" in repetitive deep diving, indicating that they independently converged on their similar specialized diving physiologies.
Beatty B L; Rothschild B M
Naturwissenschaften
2008
2008-09
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1007/s00114-008-0385-9" target="_blank" rel="noreferrer noopener">10.1007/s00114-008-0385-9</a>
Simplified method for concentration of mitochondrial membrane protein complexes
Biochemistry & Molecular Biology; blue; Blue native PAGE; Chemistry; Complex I; Electrophoretic concentration; Mitochondria; native electrophoresis; oxidative-phosphorylation; polyacrylamide-gels; sds-page
Extracting and concentrating mitochondrial protein complexes from gel strips after blue native PAGE (BN-PAGE) can be daunting tasks using the traditional methods, such as electroelution, passive diffusion and centrifugal concentration. We present a simplified gel electrophoresis method to concentrate mitochondrial protein complexes with excellent recovery rate. Mitochondrial complex I present in a long gel strip from BN-PAGE can be easily concentrated into a 0.8 cm gel strip when a second BN-PAGE is performed with a Y-shaped gel and the addition of 0.01% n-dodecyl beta-D-maltoside and 0.001% SDS in the cathode buffer. Once completed, the concentrated protein complex in the gel strip is ready for SDS-PAGE or proteomic studies.
Yeh S T; Angelos M G; Chen Y R
Electrophoresis
2010
2010-06
Journal Article
<a href="http://doi.org/10.1002/elps.201000019" target="_blank" rel="noreferrer noopener">10.1002/elps.201000019</a>