1
40
5
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1080/19336950.2016.1185579" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/19336950.2016.1185579</a>
Pages
395–409
Issue
5
Volume
10
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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TRPA1 is functionally co-expressed with TRPV1 in cardiac muscle: Co-localization at z-discs, costameres and intercalated discs.
Publisher
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Channels (Austin, Tex.)
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-09
Subject
The topic of the resource
Animals; Ca2+; Calcium/physiology; Cardiac/*physiology; cardiomyocytes; Inbred C57BL; Male; Mice; Myocytes; Transient Receptor Potential Channels/genetics/*physiology; TRPA1; TRPA1 Cation Channel; TRPV Cation Channels/genetics/*physiology; TRPV1; Z-disc
Creator
An entity primarily responsible for making the resource
Andrei Spencer R; Sinharoy Pritam; Bratz Ian N; Damron Derek S
Description
An account of the resource
Transient receptor potential channels of the ankyrin subtype-1 (TRPA1) and vanilloid subtype-1 (TRPV1) are structurally related, non-selective cation channels that show a high permeability to calcium. Previous studies indicate that TRP channels play a prominent role in the regulation of cardiovascular dynamics and homeostasis, but also contribute to the pathophysiology of many diseases and disorders within the cardiovascular system. However, no studies to date have identified the functional expression and/or intracellular localization of TRPA1 in primary adult mouse ventricular cardiomyocytes (CMs). Although TRPV1 has been implicated in the regulation of cardiac function, there is a paucity of information regarding functional expression and localization of TRPV1 in adult CMs. Our current studies demonstrate that TRPA1 and TRPV1 ion channels are co-expressed at the protein level in CMs and both channels are expressed throughout the endocardium, myocardium and epicardium. Moreover, immunocytochemical localization demonstrates that both channels predominantly colocalize at the Z-discs, costameres and intercalated discs. Furthermore, specific TRPA1 and TRPV1 agonists elicit dose-dependent, transient rises in intracellular free calcium concentration ([Ca(2+)]i) that are abolished in CMs obtained from TRPA1(-/-) and TRPV1(-/-) mice. Similarly, we observed a dose-dependent attenuation of the TRPA1 and TRPV1 agonist-induced increase in [Ca(2+)]i when WT CMs were pretreated with increasing concentrations of selective TRPA1 or TRPV1 channel antagonists. In summary, these findings demonstrate functional expression and the precise ultrastructural localization of TRPA1 and TRPV1 ion channels in freshly isolated mouse CMs. Crosstalk between TRPA1 and TRPV1 may be important in mediating cellular signaling events in cardiac muscle.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1080/19336950.2016.1185579" target="_blank" rel="noreferrer noopener">10.1080/19336950.2016.1185579</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2016
Andrei Spencer R
Animals
Bratz Ian N
Ca2+
Calcium/physiology
Cardiac/*physiology
cardiomyocytes
Channels (Austin, Tex.)
Damron Derek S
Inbred C57BL
Male
Mice
Myocytes
Sinharoy Pritam
Transient Receptor Potential Channels/genetics/*physiology
TRPA1
TRPA1 Cation Channel
TRPV Cation Channels/genetics/*physiology
TRPV1
Z-disc
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1080/19336950.2017.1365206" target="_blank" rel="noreferrer noopener">http://doi.org/10.1080/19336950.2017.1365206</a>
Pages
587–603
Issue
6
Volume
11
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
TRPA1 ion channel stimulation enhances cardiomyocyte contractile function via a CaMKII-dependent pathway.
Publisher
An entity responsible for making the resource available
Channels (Austin, Tex.)
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-11
Subject
The topic of the resource
[Ca2+]i; *Myocardial Contraction; Animals; Calcium-Calmodulin-Dependent Protein Kinase Type 2/*metabolism; CaMKII; Cardiac/*metabolism; cardiomyocytes; contractility; Inbred C57BL; Knockout; Mice; Myocytes; TRPA1; TRPA1 Cation Channel/deficiency/*metabolism
Creator
An entity primarily responsible for making the resource
Andrei Spencer R; Ghosh Monica; Sinharoy Pritam; Dey Souvik; Bratz Ian N; Damron Derek S
Description
An account of the resource
RATIONALE: Transient receptor potential channels of the ankyrin subtype-1 (TRPA1) are non-selective cation channels that show high permeability to calcium. Previous studies from our laboratory have demonstrated that TRPA1 ion channels are expressed in adult mouse ventricular cardiomyocytes (CMs) and are localized at the z-disk, costamere and intercalated disk. The functional significance of TRPA1 ion channels in the modulation of CM contractile function have not been explored. OBJECTIVE: To identify the extent to which TRPA1 ion channels are involved in modulating CM contractile function and elucidate the cellular mechanism of action. METHODS AND RESULTS: Freshly isolated CMs were obtained from murine heart and loaded with Fura-2 AM. Simultaneous measurement of intracellular free Ca(2+) concentration ([Ca(2+)]i) and contractility was performed in individual CMs paced at 0.3 Hz. Our findings demonstrate that TRPA1 stimulation with AITC results in a dose-dependent increase in peak [Ca(2+)]i and a concomitant increase in CM fractional shortening. Further analysis revealed a dose-dependent acceleration in time to peak [Ca(2+)]i and velocity of shortening as well as an acceleration in [Ca(2+)]i decay and velocity of relengthening. These effects of TRPA1 stimulation were not observed in CMs pre-treated with the TRPA1 antagonist, HC-030031 (10 micromol/L) nor in CMs obtained from TRPA1(-/-) mice. Moreover, we observed no significant increase in cAMP levels or PKA activity in response to TRPA1 stimulation and the PKA inhibitor peptide (PKI
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1080/19336950.2017.1365206" target="_blank" rel="noreferrer noopener">10.1080/19336950.2017.1365206</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
[Ca2+]i
*Myocardial Contraction
2017
Andrei Spencer R
Animals
Bratz Ian N
Calcium-Calmodulin-Dependent Protein Kinase Type 2/*metabolism
CaMKII
Cardiac/*metabolism
cardiomyocytes
Channels (Austin, Tex.)
contractility
Damron Derek S
Dey Souvik
Ghosh Monica
Inbred C57BL
Knockout
Mice
Myocytes
Sinharoy Pritam
TRPA1
TRPA1 Cation Channel/deficiency/*metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1161/atvbaha.109.186445" target="_blank" rel="noreferrer noopener">http://doi.org/10.1161/atvbaha.109.186445</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1817-1822
Issue
11
Volume
29
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Stimulation of Coronary Collateral Growth by Granulocyte Stimulating Factor Role of Reactive Oxygen Species
Publisher
An entity responsible for making the resource available
Arteriosclerosis Thrombosis and Vascular Biology
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-11
Subject
The topic of the resource
ischemia; ROS; cardiomyocytes; receptor; Cardiovascular System & Cardiology; endothelial progenitor cells; expression; Hematology; activation; angiotensin-ii; neutrophils; neovascularization; bone-marrow; coronary collateral circulation; G-CSF; G-CSF
Creator
An entity primarily responsible for making the resource
Carrao A C R; Chilian W M; Yun J; Kolz C; Rocic P; Lehmann K; van den Wijngaard Jphm; van Horssen P; Spaan J A E; Ohanyan V; Pung Y F; Buschmann I
Description
An account of the resource
Objective-The purpose of this study was to determine whether G-CSF promotes coronary collateral growth (CCG) and decipher the mechanism for this stimulation. Methods and Results-In a rat model of repetitive episodic myocardial ischemia (RI, 40 seconds LAD occlusion every 20 minutes for 2 hours and 20 minutes, 3 times/d for 5 days) CCG was deduced from collateral-dependent flow (flow to LAD region during occlusion). After RI, G-CSF (100 mu g/kg/d) increased CCG (P < 0.01) (0.47 +/- 0.15) versus vehicle (0.14 +/- 0.06). Surprisingly, G-CSF treatment without RI increased CCG (0.57 +/- 0.18) equal to G-CSF + RI. We evaluated ROS by dihydroethidine (DHE) fluorescence (LV injection, 60 mu g/kg, during two episodes of ischemia). DHE fluorescence was double in G-CSF + RI versus vehicle + RI (P < 0.01), and even higher in G-CSF without RI (P < 0.01). Interestingly, the DHE signal did not colocalize with myeloperoxidase (immunostaining, neutrophil marker) but appeared in cardiac myocytes. The study of isolated cardiac myocytes revealed the cytokine stimulates ROS which elicit production of angiogenic factors. Apocynin inhibited G-CSF effects both in vivo and in vitro. Conclusions-G-CSF stimulates ROS production directly in cardiomyocytes, which plays a pivotal role in triggering adaptations of the heart to ischemia including growth of the coronary collaterals. (Arterioscler Thromb Vasc Biol. 2009; 29: 1817-1822.)
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1161/atvbaha.109.186445" target="_blank" rel="noreferrer noopener">10.1161/atvbaha.109.186445</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2009
activation
angiotensin-ii
Arteriosclerosis Thrombosis and Vascular Biology
bone-marrow
Buschmann I
cardiomyocytes
Cardiovascular System & Cardiology
Carrao A C R
Chilian W M
coronary collateral circulation
endothelial progenitor cells
expression
G-CSF
Hematology
ischemia
Journal Article or Conference Abstract Publication
Kolz C
Lehmann K
Neovascularization
Neutrophils
Ohanyan V
Pung Y F
Receptor
Rocic P
ROS
Spaan J A E
van den Wijngaard Jphm
van Horssen P
Yun J
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajpheart.00019.2011" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajpheart.00019.2011</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
H270-H277
Issue
1
Volume
302
Search for Full-text
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Optical mapping of cryoinjured rat myocardium grafted with mesenchymal stem cells
Publisher
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American Journal of Physiology-Heart and Circulatory Physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-01
Subject
The topic of the resource
cardiomyocytes; Myocardial infarction; therapy; Physiology; Cardiovascular System & Cardiology; in-vitro; repair; Transplantation; phenotype; regeneration; infarction; heart; stem cell; action potential; pacemakers
Creator
An entity primarily responsible for making the resource
Costa A R; Panda N C; Yong S; Mayorga M E; Pawlowski G P; Fan K K; Penn M S; Laurita K R
Description
An account of the resource
Costa AR, Panda NC, Yong S, Mayorga ME, Pawlowski GP, Fan K, Penn MS, Laurita KR. Optical mapping of cryoinjured rat myocardium grafted with mesenchymal stem cells. Am J Physiol Heart Circ Physiol 302: H270-H277, 2012. First published October 28, 2011; doi: 10.1152/ajpheart.00019.2011.-Mesenchymal stem cells (MSCs) have been shown to improve cardiac electrophysiology when administered in the setting of acute myocardial infarction. However, the electrophysiological phenotype of MSCs in situ is not clear. We hypothesize that MSCs delivered intramyocardially to cryoinjured myocardium can engraft, but will not actively generate, action potentials. Cryoinjury-induced scar was created on the left ventricular epicardial surface of adult rat hearts. Within 30 min, hearts were injected with saline (sham, n = 11) or bone marrow-derived MSCs (2 x 10(6)) labeled with 1,1'-dioctadecyl-3,3,3,3'-tetramethyl-indocarbocyanine percholate (DiI; n = 16). At 3 wk, optical mapping and cell isolation were used to measure optical action potentials and calcium transients, respectively. Histological analysis confirmed subepicardial scar thickness and the presence of DiI-positive cells that express connexin-43. Optical action potential amplitude within the scar at MSC-positive sites (53.8 +/- 14.3%) was larger compared with sites devoid of MSCs (35.3 +/- 14.2%, P < 0.05) and sites within the scar of shams (33.5 +/- 6.9%, P < 0.05). Evidence of simultaneous action potential upstroke, the loss of action potential activity following ablation of adjacent viable myocardium, and no rapid calcium transient response in isolated DiI + cells suggest that the electrophysiological influence of engrafted MSCs is electrotonic. MSCs can engraft when directly injected into a cryoinjury and are associated with evidence of action potential activity. However, our results suggest that this activity is not due to generation of action potentials, but rather passive influence coupled from neighboring viable myocardium.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/ajpheart.00019.2011" target="_blank" rel="noreferrer noopener">10.1152/ajpheart.00019.2011</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2012
action potential
American Journal of Physiology-Heart and Circulatory Physiology
cardiomyocytes
Cardiovascular System & Cardiology
Costa A R
Fan K K
heart
in-vitro
Infarction
Journal Article or Conference Abstract Publication
Laurita K R
Mayorga M E
myocardial infarction
pacemakers
Panda N C
Pawlowski G P
Penn M S
Phenotype
Physiology
Regeneration
repair
stem cell
therapy
Transplantation
Yong S
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.3109/14653249.2012.684380" target="_blank" rel="noreferrer noopener">http://doi.org/10.3109/14653249.2012.684380</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
983-993
Issue
8
Volume
14
Search for Full-text
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Cardiac Pressure Overload Initiates A Systemic Stem Cell Response
Publisher
An entity responsible for making the resource available
Cytotherapy
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-09
Subject
The topic of the resource
& Experimental Medicine; acute myocardial-infarction; Biotechnology & Applied Microbiology; bone marrow; bone marrow; cardiac stem cells; cardiomyocytes; Cell Biology; endogenous stem cells; endothelial; endothelial progenitor cells; heart; Hematology; hypertrophy; identification; murine; peripheral-blood; progenitor cells; regeneration; Research; spleen; SSEA-1; transaortic constriction; transplantation
Creator
An entity primarily responsible for making the resource
Finan A; Kiedrowski M; Turturice B A; Sopko N A; Penn M S
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.3109/14653249.2012.684380" target="_blank" rel="noreferrer noopener">10.3109/14653249.2012.684380</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
& Experimental Medicine
2012
acute myocardial-infarction
Biotechnology & Applied Microbiology
bone marrow
cardiac stem cells
cardiomyocytes
Cell Biology
Cytotherapy
endogenous stem cells
Endothelial
endothelial progenitor cells
Finan A
heart
Hematology
Hypertrophy
identification
Kiedrowski M
murine
Penn M S
peripheral-blood
progenitor cells
Regeneration
Research
Sopko N A
spleen
SSEA-1
transaortic constriction
Transplantation
Turturice B A