1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1002/jcp.22077" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jcp.22077</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Pages 519-529 Issue 2 Volume 223 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Differential Expression and Cellular Distribution of gamma-Tubulin and beta III-Tubulin in Medulloblastomas and Human Medulloblastoma Cell Lines Publisher An entity responsible for making the resource available Journal of Cellular Physiology Date A point or period of time associated with an event in the lifecycle of the resource 2010 2010-05 Subject The topic of the resource ring; Physiology; Cell Biology; complex; mammalian-cells; monoclonal-antibodies; candidate genes; cerebellar medulloblastomas; kinesin-like protein; microtubule nucleation; mitotic spindle; pale islands; posttranslational modification Creator An entity primarily responsible for making the resource Caracciolo V; D'Agostino L; Draberova E; Sladkova V; Crozier-Fitzgerald C; Agamanolis D P; De Chadarevian J P; Legido A; Giordano A; Draber P; Katsetos C D Description An account of the resource In previous studies, we have shown overexpression and ectopic subcellular distribution of gamma-tubulin and beta III-tubulin in human glioblastomas and glioblastoma cell lines (Katsetos et al., 2006,] Neuropathol Exp Neurol 65:455-467; Katsetos et al., 2007, Neurochem Res 32:1387-1398). Here we determined the expression of gamma-tubulin in surgically excised medulloblastomas (n = 20) and in the human medulloblastoma cell lines D283 Med and DAOY. In clinical tissue samples, the immunohistochemical distribution of gamma-tubulin labeling was pervasive and inversely related to neuritogenesis. Overexpression of gamma-tubulin was widespread in poorly differentiated, proliferating tumor cells but was significantly diminished in quiescent differentiating tumor cells undergoing neuritogenesis, highlighted by beta III-tubulin immunolabeling. By quantitative real-time PCR, gamma-tubulin transcripts for TUBG1, TUBG2, and TUBB3 genes were detected in both cell lines but expression was less prominent when compared with the human glioblastoma cell lines. Immunoblotting revealed comparable amounts of gamma-tubulin and beta III-tubulin in different phases of cell cycle; however, a larger amount of gamma-tubulin was detected in D283 Med when compared with DAOY cells. Interphase D283 Med cells exhibited predominantly diffuse cytoplasmic gamma-tubulin localization, in addition to the expected centrosome-associated distribution. Robust beta III-tubulin immunoreactivity was detected in mitotic spindles of DAOY cells. Our data indicate that overexpression of gamma-tubulin may be linked to phenotypic dedifferentiation (anaplasia) and tumor progression in medulloblastomas and may potentially serve as a promising tumor marker. J. Cell. Physiol. 223: 519-529,2010. (C) 2010 Wiley-Liss, Inc. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1002/jcp.22077" target="_blank" rel="noreferrer noopener">10.1002/jcp.22077</a> Format The file format, physical medium, or dimensions of the resource Journal Article or Conference Abstract Publication 2010 Agamanolis D P candidate genes Caracciolo V Cell Biology cerebellar medulloblastomas complex Crozier-Fitzgerald C D'Agostino L De Chadarevian J P Draber P Draberova E Giordano A Journal Article or Conference Abstract Publication Journal of cellular physiology Katsetos C D kinesin-like protein Legido A mammalian-cells microtubule nucleation mitotic spindle monoclonal-antibodies pale islands Physiology posttranslational modification ring Sladkova V